The present disclosure relates to compositions, methods and kits for labeling an internal sequence of a target nucleic acid molecule with molecular barcodes. In some embodiments, the methods comprise intramolecular circulation of a labeled target nucleic acid molecule. Further provided methods for generating sequencing libraries comprising overlapping fragments covering the full length of a target nucleic acid molecule, sequencing the libraries using the methods disclosed herein, and methods of analyzing sequencing results therefrom.

Patent
   10640763
Priority
May 31 2016
Filed
May 16 2017
Issued
May 05 2020
Expiry
Mar 20 2038
Extension
308 days
Assg.orig
Entity
Large
0
743
currently ok
1. A method of labeling a target nucleic acid sequence in a sample with a molecular barcode, comprising:
hybridizing an oligonucleotide comprising a molecular barcode with a first nucleic acid molecule comprising the target nucleic acid sequence;
extending the oligonucleotide to generate a second nucleic acid molecule comprising the molecular barcode and the target nucleic acid sequence;
circularizing the second nucleic acid molecule or complement thereof to generate a circularized nucleic acid molecule comprising the molecular barcode in close proximity to the target nucleic acid sequence; and
amplifying the circularized nucleic acid molecule to generate a plurality of amplicons comprising the molecular barcode in close proximity to the target nucleic acid sequence, wherein one or more amplification reactions use at least one primer comprising a binding site for a sequencing primer, wherein the sequencing primer does not comprise a sequence homologous to the sequence of the first nucleic acid molecule.
2. The method of claim 1, further comprising synthesizing a complementary strand of the second nucleic acid molecule to generate a double-stranded nucleic acid molecule.
3. The method of claim 2, wherein the circularizing comprises circularizing the double-stranded nucleic acid molecule.
4. The method of claim 1, further comprising amplifying the second nucleic acid molecule or complement thereof to generate a copy of the second nucleic acid molecule or complement thereof.
5. The method of claim 4, wherein the circularizing comprises circularizing a copy of the second nucleic acid molecule or complement thereof.
6. The method of claim 1, further comprising sequencing the plurality of amplicons.
7. The method of claim 1, wherein the first nucleic acid is an mRNA.
8. The method of claim 1, wherein the oligonucleotide specifically binds to a binding site on the first nucleic acid molecule.
9. The method of claim 8, wherein the binding site is a gene-specific sequence.
10. The method of claim 8, wherein the binding site is a poly-A sequence.
11. The method of claim 1, wherein the target nucleic acid sequence comprises 20 nt to 30 nt.
12. The method of claim 1, wherein the target nucleic acid sequence comprises 30 nt to 40 nt.
13. The method of claim 1, wherein the target nucleic acid sequence comprises 40 nt to 50 nt.
14. The method of claim 8, wherein the binding site is at least 200 nt away from the target nucleic acid sequence on the first nucleic acid molecule.
15. The method of claim 8, wherein the binding site is at least 500 nt away from the target nucleic acid sequence on the first nucleic acid molecule.
16. The method of claim 8, wherein the binding site is at least 1,000 nt away from the target nucleic acid sequence on the first nucleic acid molecule.
17. The method of claim 8, wherein the binding site is at least 2,000 nt away from the target nucleic acid sequence on the first nucleic acid molecule.
18. The method of claim 1, wherein the molecular barcode comprises a sample label, a cellular label, a molecular label, or a combination thereof.
19. The method of claim 1, wherein the molecular barcode comprises a binding site for a primer.
20. The method of claim 19, wherein the primer is a universal primer.
21. The method of claim 1, wherein the sequencing primer is a sequence associated with a high-throughput sequencing platform.

The present application claims priority under 35 U.S.C. §119(e) to U.S. Provisional Application No. 62/343,574, filed on May 31, 2016, which is herein expressly incorporated by reference in its entirety.

The present application is being filed along with a Sequence Listing in electronic format. The Sequence Listing is provided as a file entitled BDCRI-021PR_Sequence_Listing.TXT, created May 31, 2016, which is 2 Kb in size. The information in the electronic format of the Sequence Listing is incorporated herein by reference in its entirety.

Current methods of molecular barcoding and sequence analysis are typically limited to the 3′end of the target transcript, because molecular barcodes were attached to the 3′ end, and Illumina sequencing length is short. Molecular barcoding of sequences upstream of transcript 3′end can be performed using gene-specific reverse transcription (RT) primers, the scalability is limited because RT primer with large amounts of molecular barcodes are designed against each gene, making it expensive to manufacture barcoded primers in a large gene pool. Methods for long read sequencing are limited by high sequencing error rates, low read throughput, and absence of molecular barcoding, which are aspects that prevent accurate sequence analysis, quantification, and lack of scalability.

Some embodiments disclosed herein provide methods of labeling a target nucleic acid in a sample with a molecular barcode, comprising: hybridizing an oligonucleotide comprising a molecular barcode with a first nucleic acid molecule comprising the target nucleic acid; extending the oligonucleotide to generate a second nucleic acid molecule comprising the molecular barcode and the target nucleic acid; circularizing the second nucleic acid molecule or complement thereof to generate a circularized nucleic acid molecule comprising the molecular barcode in close proximity to the target nucleic acid; and amplifying the circularized nucleic acid molecule to generate a plurality of amplicons comprising the molecular barcode in close proximity to the target nucleic acid. In some embodiments, the methods further comprise synthesizing a complementary strand of the second nucleic acid molecule to generate a double-stranded nucleic acid molecule. In some embodiments, the circularizing comprises circularizing the double-stranded nucleic acid molecule. In some embodiments, the methods further comprise amplifying the second nucleic acid molecule or complement thereof to generate a copy of the second nucleic acid molecule or complement thereof. In some embodiments, the circularizing comprises circularizing a copy of the second nucleic acid molecule or complement thereof. In some embodiments, the methods further comprise sequencing the plurality of amplicons. In some embodiments, the first nucleic acid is an mRNA. In some embodiments, the oligonucleotide specifically binds to a binding site on the first nucleic acid molecule. In some embodiments, the binding site is a gene-specific sequence. In some embodiments, the binding site is a poly-A sequence. In some embodiments, the target nucleic acid comprises about 20 nt. In some embodiments, the target nucleic acid comprises about 30 nt. In some embodiments, the target nucleic acid comprises about 40 nt. In some embodiments, the binding site is at least 200 nt away from the target nucleic acid on the first nucleic acid molecule. In some embodiments, the binding site is at least 500 nt away from the target nucleic acid on the first nucleic acid molecule. In some embodiments, the binding site is at least 1,000 nt away from the target nucleic acid on the first nucleic acid molecule. In some embodiments, the binding site is at least 2,000 nt away from the target nucleic acid on the first nucleic acid molecule. In some embodiments, the molecular barcode comprises a sample label, a cellular label, a molecular label, or a combination thereof. In some embodiments, the molecular barcode comprises a binding site for a primer. In some embodiments, the primer is a universal primer. In some embodiments, the amplifying the circularized nucleic acid molecule comprises PCR amplification using a target-specific primer that specifically binds to the target nucleic acid or complement thereof. In some embodiments, the methods further comprise ligating an adaptor to the second nucleic acid molecule or complement thereof before the circularizing step. In some embodiments, the adaptor comprises a binding site for a second universal primer. In some embodiments, the amplifying the second nucleic acid molecule or complement thereof comprises PCR amplification using a second universal primer. In some embodiments, the target nucleic acid is a complementarity determining region (CDR) coding region of a T cell receptor gene. In some embodiments, the target nucleic acid is a complementarity determining region (CDR) coding region of an immunoglobulin gene. In some embodiments, the sample comprises a single cell. In some embodiments, the sample comprises a plurality of cells. In some embodiments, the oligonucleotide is immobilized on a solid support. In some embodiments, the solid support is a bead.

Some embodiments disclosed herein provide methods of labeling a target nucleic acid in a sample with a molecular barcode, comprising: hybridizing an oligonucleotide comprising a molecular barcode with a first nucleic acid molecule comprising the target nucleic acid; extending the oligonucleotide to generate a second nucleic acid molecule comprising the molecular barcode and the target nucleic acid; amplifying the second nucleic acid molecule or complement thereof to generate a first plurality of amplicons comprising the molecular barcode and the target nucleic acid; circularizing the first plurality of amplicons to generate a circularized nucleic acid molecule comprising the molecular barcode in close proximity to the target nucleic acid; and amplifying the circularized nucleic acid molecule to generate a second plurality of amplicons comprising the molecular barcode in close proximity to the target nucleic acid. In some embodiments, the methods further comprise sequencing the second plurality of amplicons. In some embodiments, the first nucleic acid is an mRNA. In some embodiments, the oligonucleotide specifically binds to a binding site on the first nucleic acid molecule. In some embodiments, the binding site is a gene-specific sequence. In some embodiments, the binding site is a poly-A sequence. In some embodiments, target nucleic acid comprises about 20 nt. In some embodiments, the target nucleic acid comprises about 30 nt. In some embodiments, the target nucleic acid comprises about 40 nt. In some embodiments, the binding site is at least 200 nt away from the target nucleic acid on the first nucleic acid molecule. In some embodiments, the binding site is at least 500 nt away from the target nucleic acid on the first nucleic acid molecule. In some embodiments, the binding site is at least 1,000 nt away from the target nucleic acid on the first nucleic acid molecule. In some embodiments, the binding site is at least 2,000 nt away from the target nucleic acid on the first nucleic acid molecule. In some embodiments, the molecular barcode comprises a sample label, a cellular label, a molecular label, or a combination thereof. In some embodiments, the molecular barcode comprises a binding site for a primer. In some embodiments, the primer is a universal primer. In some embodiments, the amplifying the circularized nucleic acid molecule comprises PCR amplification using a target-specific primer that specifically binds to the target nucleic acid or complement thereof. In some embodiments, the methods further comprise ligating an adaptor to the second nucleic acid molecule or complement thereof before the amplifying the second nucleic acid molecule or complement thereof step. In some embodiments, the adaptor comprises a binding site for a second universal primer. In some embodiments, the amplifying the second nucleic acid molecule or complement thereof comprises PCR amplification using a second universal primer. In some embodiments, the target nucleic acid is a complementarity determining region (CDR) coding region of a T cell receptor gene. In some embodiments, the target nucleic acid is a complementarity determining region (CDR) coding region of an immunoglobulin gene. In some embodiments, the sample comprises a single cell. In some embodiments, the sample comprises a plurality of cells. In some embodiments, the oligonucleotide is immobilized on a solid support. In some embodiments, the solid support is a bead.

Some embodiments disclosed herein provide methods of generating a sequencing library for a target nucleic acid molecule from a sample, comprising: hybridizing the target nucleic acid molecule with an oligonucleotide comprising a molecular barcode; extending the oligonucleotide to generate a second nucleic acid molecule comprising the molecular barcode and the target nucleic acid molecule; amplifying the second nucleic acid molecule or complement thereof to generate a first plurality of amplicons comprising the molecular barcode and the target nucleic acid molecule; fragmenting the first plurality of amplicons to generate a plurality of nucleic acid fragments comprising the molecular barcode and fragments of the target nucleic acid molecule, wherein at least two of the fragments of the target nucleic acid molecule have different length; circularizing the plurality of nucleic acid fragments to generate a plurality of circularized nucleic acid molecules, wherein at least two of the plurality of circularized nucleic acid molecules comprise the molecular barcode in close proximity to different positions of the target nucleic acid molecule; and amplifying the plurality of circularized nucleic acid molecules to generate a second plurality of amplicons, wherein at least two of the second plurality of amplicons comprises the molecular barcode in close proximity to different positions of the target nucleic acid molecule. In some embodiments, each of the plurality of nucleic acid fragments has a length from 50 nt to 10,000 nt. In some embodiments, the plurality of nucleic acid fragments comprises at least 2 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 10 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 100 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 1,000 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 10,000 nucleic acid fragments. In some embodiments, the fragmenting comprises sonication of the first plurality of amplicons. In some embodiments, the fragmenting comprises restriction digestion of the first plurality of amplicons. In some embodiments, at least 50% of the plurality of nucleic acid fragments comprises different length. In some embodiments, at least 80% of the plurality of nucleic acid fragments comprises different length. In some embodiments, at least 90% of the plurality of nucleic acid fragments comprises different length. In some embodiments, the target nucleic acid is a DNA. In some embodiments, the target nucleic acid is an mRNA. In some embodiments, the oligonucleotide specifically binds to a binding site on the target nucleic acid molecule. In some embodiments, the binding site is a gene-specific sequence. In some embodiments, the binding site is a poly-A sequence. In some embodiments, the molecular barcode comprises a sample label, a cellular label, a molecular label, or a combination thereof. In some embodiments, the molecular barcode comprises a binding site for a primer. In some embodiments, the primer is a universal primer. In some embodiments, the amplifying the plurality of circularized nucleic acid molecules comprises PCR amplification using the universal primer. In some embodiments, the methods further comprise ligating an adaptor to the second nucleic acid molecule or complement thereof before the amplifying step. In some embodiments, the adaptor comprises a binding site for a second universal primer. In some embodiments, the amplifying the second nucleic acid molecule or complement thereof comprises PCR amplification using a second universal primer. In some embodiments, the methods further comprise amplifying the second plurality of amplicons to generate a third plurality of amplicons. In some embodiments, the amplifying the second plurality of amplicons comprises PCR amplification using a random primer. In some embodiments, the random primer comprises a binding site for a sequencing primer. In some embodiments, at least two of the third plurality of amplicons overlap with each other. In some embodiments, the at least two of the third plurality of amplicons overlap with each other by at least 8 nt. In some embodiments, the at least two of the third plurality of amplicons overlap with each other by at least 10 nt. In some embodiments, the at least two of the third plurality of amplicons overlap with each other by at least 12 nt. In some embodiments, the at least two of the third plurality of amplicons overlap with each other by at least 14 nt. In some embodiments, the third plurality of amplicons covers the entire length of the nucleic acid molecule. In some embodiments, the third plurality of amplicons has an average size of about 250 nt. In some embodiments, the sample comprises a single cell. In some embodiments, the sample comprises a plurality of cells. In some embodiments, the oligonucleotide is immobilized on a solid support. In some embodiments, the solid support is a bead. In some embodiments, the methods further comprise generating a sequencing library for a plurality of target nucleic acid molecules from the sample.

Some embodiments disclosed herein provide compositions for generating a sequencing library for a plurality of nucleic acid molecules of a sample, comprising a plurality of oligonucleotides, wherein each of the plurality of oligonucleotides comprises from 5′ to 3′: a molecular label, a sample label, a binding site for a sequencing primer and a target-specific region that specifically binds to a nucleic acid molecule, wherein each of the plurality of oligonucleotides comprises the same sample label, and wherein at least 100 of the plurality of oligonucleotides comprise different molecular labels. In some embodiments, the binding site for the sequencing primer is oriented in the opposite direction of the oligonucleotide. In some embodiments, the target-specific region binds to each of the plurality of nucleic acid molecules. In some embodiments, the target-specific region comprises a random sequence. In some embodiments, the target-specific region comprises an oligo-dT sequence. In some embodiments, each of the plurality of oligonucleotides comprises a restriction enzyme recognition site 5′ to the molecular label. In some embodiments, each of the plurality of oligonucleotides comprises a binding site for a universal primer 5′ to the molecular label. In some embodiments, the binding site for a universal primer is 5′ to the restriction enzyme recognition site. In some embodiments, the sample comprises a single cell. In some embodiments, the sample comprises a plurality of cells. In some embodiments, the oligonucleotide is immobilized on a solid support. In some embodiments, the solid support is a bead.

Some embodiments disclosed herein provide kits for generating a sequencing library for a plurality of nucleic acid molecules of a sample, comprising a plurality of oligonucleotides and an enzyme, wherein each of the plurality of oligonucleotides comprises from 5′ to 3′: a molecular label, a sample label, a binding site for a sequencing primer and a target-specific region that specifically binds to a nucleic acid molecule, wherein each of the plurality of oligonucleotides comprises the same sample label, and wherein at least 100 of the plurality of oligonucleotides comprise different molecular labels. In some embodiments, the binding site for a sequencing primer is oriented in the opposite direction of the oligonucleotide. In some embodiments, the target-specific region binds to each of the plurality of nucleic acid molecules. In some embodiments, the target-specific region comprises a random sequence. In some embodiments, the target-specific region comprises an oligo-dT sequence. In some embodiments, each of the plurality of oligonucleotides comprises a restriction enzyme recognition site 5′ to the molecular label. In some embodiments, each of the plurality of oligonucleotides comprises a binding site for a universal primer 5′ to the molecular label. In some embodiments, the binding site for a universal primer is 5′ to the restriction enzyme recognition site. In some embodiments, the enzyme is selected from the group consisting of a ligase, a restriction enzyme, a DNA polymerase, a reverse transcriptase, an RNase, or any combination thereof. In some embodiments, the kits further comprise an adaptor. In some embodiments, the adaptor comprises a binding site for a second universal primer. In some embodiments, the kits further comprise a random primer. In some embodiments, the random primer comprises a binding site for a second sequencing primer. In some embodiments, the sample comprises a single cell. In some embodiments, the sample comprises a plurality of cells. In some embodiments, the oligonucleotide is immobilized on a solid support. In some embodiments, the solid support comprises a bead.

Some embodiments disclosed herein provide sequencing libraries for a nucleic acid molecule from a sample comprising a plurality of amplicons, wherein each of the plurality of amplicons comprises from 5′ to 3′: a binding site for a first sequencing primer, a molecular label, a fragment of the nucleic acid molecule and a binding site for a second sequencing primer, wherein each of the plurality of amplicons comprises the same molecular label, and wherein the fragments of the nucleic acid molecule of the plurality of amplicons cover the entire length of the nucleic acid molecule. In some embodiments, each of the plurality of amplicons comprises a sample label. In some embodiments, each of the plurality of amplicons comprises the same sample label. In some embodiments, the plurality of amplicons comprises an average size of 250 nt. In some embodiments, the plurality of amplicons comprises an average size of 500 nt. In some embodiments, the nucleic acid molecule is an mRNA. In some embodiments, the nucleic acid molecule has a length of at least 1,500 nt. In some embodiments, the nucleic acid molecule has a length of at least 3,000 nt. In some embodiments, the nucleic acid molecule has a length of at least 5,000 nt. In some embodiments, the sample comprises a single cell. In some embodiments, the sequencing libraries comprise at least 10 amplicons. In some embodiments, the sequencing libraries comprise at least 20 amplicons. In some embodiments, the sequencing libraries comprise at least 50 amplicons. In some embodiments, the sequencing libraries comprise at least 100 amplicons. In some embodiments, the sequencing libraries comprise at least 200 amplicons. In some embodiments, the sequencing libraries comprise at least 500 amplicons. In some embodiments, at least two of the fragments of the nucleic acid molecule overlap with each other. In some embodiments, the at least two of the fragments of the nucleic acid molecule overlap with each other by at least 8 nt. In some embodiments, the at least two of the fragments of the nucleic acid molecule overlap with each other by at least 10 nt. In some embodiments, the at least two of the fragments of the nucleic acid molecule overlap with each other by at least 12 nt. In some embodiments, the at least two of the fragments of the nucleic acid molecule overlap with each other by at least 14 nt.

FIG. 1 shows a schematic illustration of an exemplary method of labeling a target nucleic acid with a molecular barcode.

FIG. 2 shows a schematic illustration of an exemplary method of generating a sequencing library for a target nucleic acid molecule.

FIG. 3 shows a schematic illustration of an exemplary method of generating a sequencing library for a target nucleic acid molecule.

FIG. 4 shows sequencing results from 1 ng T cell RNA using the method disclosed herein.

Definitions

Unless otherwise defined, all technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art in the field to which this disclosure belongs. As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural references unless the context clearly dictates otherwise. Any reference to “or” herein is intended to encompass “and/or” unless otherwise stated.

As used herein the term “associated” or “associated with” can mean that two or more species are identifiable as being co-located at a point in time. An association can mean that two or more species are or were within a similar container. An association can be an informatics association, where for example digital information regarding two or more species is stored and can be used to determine that one or more of the species were co-located at a point in time. An association can also be a physical association. In some instances two or more associated species are “tethered”, “attached”, or “immobilized” to one another or to a common solid or semisolid surface. An association may refer to covalent or non-covalent means for attaching labels to solid or semi-solid supports such as beads. An association may comprise hybridization between a target and a label.

As used herein, the term “complementary” can refer to the capacity for precise pairing between two nucleotides. For example, if a nucleotide at a given position of a nucleic acid is capable of hydrogen bonding with a nucleotide of another nucleic acid, then the two nucleic acids are considered to be complementary to one another at that position. Complementarity between two single-stranded nucleic acid molecules may be “partial,” in which only some of the nucleotides bind, or it may be complete when total complementarity exists between the single-stranded molecules. A first nucleotide sequence can be said to be the “complement” of a second sequence if the first nucleotide sequence is complementary to the second nucleotide sequence. A first nucleotide sequence can be said to be the “reverse complement” of a second sequence, if the first nucleotide sequence is complementary to a sequence that is the reverse (i.e., the order of the nucleotides is reversed) of the second sequence. As used herein, the terms “complement”, “complementary”, and “reverse complement” can be used interchangeably. It is understood from the disclosure that if a molecule can hybridize to another molecule it may be the complement of the molecule that is hybridizing.

As used herein, the term “digital counting” can refer to a method for estimating a number of target molecules in a sample. Digital counting can include the step of determining a number of unique labels that have been associated with targets in a sample. This stochastic methodology transforms the problem of counting molecules from one of locating and identifying identical molecules to a series of yes/no digital questions regarding detection of a set of predefined labels.

As used herein, the term “label” or “labels” can refer to nucleic acid codes associated with a target within a sample. A label can be, for example, a nucleic acid label. A label can be an entirely or partially amplifiable label. A label can be entirely or partially sequencable label. A label can be a portion of a native nucleic acid that is identifiable as distinct. A label can be a known sequence. A label can comprise a junction of nucleic acid sequences, for example a junction of a native and non-native sequence. As used herein, the term “label” can be used interchangeably with the terms, “index”, “tag,” or “label-tag.” Labels can convey information. For example, in various embodiments, labels can be used to determine an identity of a sample, a source of a sample, an identity of a cell, and/or a target.

As used herein, a “nucleic acid” can generally refer to a polynucleotide sequence, or fragment thereof. A nucleic acid can comprise nucleotides. A nucleic acid can be exogenous or endogenous to a cell. A nucleic acid can exist in a cell-free environment. A nucleic acid can be a gene or fragment thereof. A nucleic acid can be DNA. A nucleic acid can be RNA. A nucleic acid can comprise one or more analogs (e.g. altered backgone, sugar, or nucleobase). Some non-limiting examples of analogs include: 5-bromouracil, peptide nucleic acid, xeno nucleic acid, morpholinos, locked nucleic acids, glycol nucleic acids, threose nucleic acids, dideoxynucleotides, cordycepin, 7-deaza-GTP, florophores (e.g. rhodamine or flurescein linked to the sugar), thiol containing nucleotides, biotin linked nucleotides, fluorescent base analogs, CpG islands, methyl-7-guanosine, methylated nucleotides, inosine, thiouridine, pseudourdine, dihydrouridine, queuosine, and wyosine. “Nucleic acid”, “polynucleotide, “target polynucleotide”, and “target nucleic acid” can be used interchangeably.

A nucleic acid can comprise one or more modifications (e.g., a base modification, a backbone modification), to provide the nucleic acid with a new or enhanced feature (e.g., improved stability). A nucleic acid can comprise a nucleic acid affinity tag. A nucleoside can be a base-sugar combination. The base portion of the nucleoside can be a heterocyclic base. The two most common classes of such heterocyclic bases are the purines and the pyrimidines. Nucleotides can be nucleosides that further include a phosphate group covalently linked to the sugar portion of the nucleoside. For those nucleosides that include a pentofuranosyl sugar, the phosphate group can be linked to the 2′, the 3′, or the 5′ hydroxyl moiety of the sugar. In forming nucleic acids, the phosphate groups can covalently link adjacent nucleosides to one another to form a linear polymeric compound. In turn, the respective ends of this linear polymeric compound can be further joined to form a circular compound; however, linear compounds are generally suitable. In addition, linear compounds may have internal nucleotide base complementarity and may therefore fold in a manner as to produce a fully or partially double-stranded compound. Within nucleic acids, the phosphate groups can commonly be referred to as forming the internucleoside backbone of the nucleic acid. The linkage or backbone of the nucleic acid can be a 3′ to 5′ phosphodiester linkage.

A nucleic acid can comprise a modified backbone and/or modified internucleoside linkages. Modified backbones can include those that retain a phosphorus atom in the backbone and those that do not have a phosphorus atom in the backbone. Suitable modified nucleic acid backbones containing a phosphorus atom therein can include, for example, phosphorothioates, chiral phosphorothioates, phosphorodithioates, phosphotriesters, aminoalkylphosphotriesters, methyl and other alkyl phosphonates such as 3′-alkylene phosphonates, 5′-alkylene phosphonates, chiral phosphonates, phosphinates, phosphoramidates including 3′-amino phosphoramidate and aminoalkylphosphoramidates, phosphorodiamidates, thionophosphoramidates, thionoalkylphosphonates, thionoalkylphosphotriesters, selenophosphates, and boranophosphates having normal 3′-5′ linkages, 2′-5′ linked analogs, and those having inverted polarity wherein one or more internucleotide linkages is a 3′ to 3′, a 5′ to 5′ or a 2′ to 2′ linkage.

A nucleic acid can comprise polynucleotide backbones that are formed by short chain alkyl or cycloalkyl internucleoside linkages, mixed heteroatom and alkyl or cycloalkyl internucleoside linkages, or one or more short chain heteroatomic or heterocyclic internucleoside linkages. These can include those having morpholino linkages (formed in part from the sugar portion of a nucleoside); siloxane backbones; sulfide, sulfoxide and sulfone backbones; formacetyl and thioformacetyl backbones; methylene formacetyl and thioformacetyl backbones; riboacetyl backbones; alkene containing backbones; sulfamate backbones; methyleneimino and methylenehydrazino backbones; sulfonate and sulfonamide backbones; amide backbones; and others having mixed N, O, S and CH2 component parts.

A nucleic acid can comprise a nucleic acid mimetic. The term “mimetic” can be intended to include polynucleotides wherein only the furanose ring or both the furanose ring and the internucleotide linkage are replaced with non-furanose groups, replacement of only the furanose ring can also be referred as being a sugar surrogate. The heterocyclic base moiety or a modified heterocyclic base moiety can be maintained for hybridization with an appropriate target nucleic acid. One such nucleic acid can be a peptide nucleic acid (PNA). In a PNA, the sugar-backbone of a polynucleotide can be replaced with an amide containing backbone, in particular an aminoethylglycine backbone. The nucleotides can be retained and are bound directly or indirectly to aza nitrogen atoms of the amide portion of the backbone. The backbone in PNA compounds can comprise two or more linked aminoethylglycine units which gives PNA an amide containing backbone. The heterocyclic base moieties can be bound directly or indirectly to aza nitrogen atoms of the amide portion of the backbone.

A nucleic acid can comprise a morpholino backbone structure. For example, a nucleic acid can comprise a 6-membered morpholino ring in place of a ribose ring. In some of these embodiments, a phosphorodiamidate or other non-phosphodiester internucleoside linkage can replace a phosphodiester linkage.

A nucleic acid can comprise linked morpholino units (i.e. morpholino nucleic acid) having heterocyclic bases attached to the morpholino ring. Linking groups can link the morpholino monomeric units in a morpholino nucleic acid. Non-ionic morpholino-based oligomeric compounds can have less undesired interactions with cellular proteins. Morpholino-based polynucleotides can be nonionic mimics of nucleic acids. A variety of compounds within the morpholino class can be joined using different linking groups. A further class of polynucleotide mimetic can be referred to as cyclohexenyl nucleic acids (CeNA). The furanose ring normally present in a nucleic acid molecule can be replaced with a cyclohexenyl ring. CeNA DMT protected phosphoramidite monomers can be prepared and used for oligomeric compound synthesis using phosphoramidite chemistry. The incorporation of CeNA monomers into a nucleic acid chain can increase the stability of a DNA/RNA hybrid. CeNA oligoadenylates can form complexes with nucleic acid complements with similar stability to the native complexes. A further modification can include Locked Nucleic Acids (LNAs) in which the 2′-hydroxyl group is linked to the 4′ carbon atom of the sugar ring thereby forming a 2′-C,4′-C-oxymethylene linkage thereby forming a bicyclic sugar moiety. The linkage can be a methylene (—CH2-), group bridging the 2′ oxygen atom and the 4′ carbon atom wherein n is 1 or 2. LNA and LNA analogs can display very high duplex thermal stabilities with complementary nucleic acid (Tm=+3 to +10° C.), stability towards 3′-exonucleolytic degradation and good solubility properties.

A nucleic acid may also include nucleobase (often referred to simply as “base”) modifications or substitutions. As used herein, “unmodified” or “natural” nucleobases can include the purine bases, (e.g. adenine (A) and guanine (G)), and the pyrimidine bases, (e.g. thymine (T), cytosine (C) and uracil (U)). Modified nucleobases can include other synthetic and natural nucleobases such as 5-methylcytosine (5-me-C), 5-hydroxymethyl cytosine, xanthine, hypoxanthine, 2-aminoadenine, 6-methyl and other alkyl derivatives of adenine and guanine, 2-propyl and other alkyl derivatives of adenine and guanine, 2-thiouracil, 2-thiothymine and 2-thiocytosine, 5-halouracil and cytosine, 5-propynyl (—C═C—CH3) uracil and cytosine and other alkynyl derivatives of pyrimidine bases, 6-azo uracil, cytosine and thymine, 5-uracil (pseudouracil), 4-thiouracil, 8-halo, 8-amino, 8-thiol, 8-thioalkyl, 8-hydroxyl and other 8-substituted adenines and guanines, 5-halo particularly 5-bromo, 5-trifluoromethyl and other 5-substituted uracils and cytosines, 7-methylguanine and 7-methyladenine, 2-F-adenine, 2-aminoadenine, 8-azaguanine and 8-azaadenine, 7-deazaguanine and 7-deazaadenine and 3-deazaguanine and 3-deazaadenine. Modified nucleobases can include tricyclic pyrimidines such as phenoxazine cytidine(1H-pyrimido(5,4-b)(1,4)benzoxazin-2(3H)-one), phenothiazine cytidine (1H-pyrimido(5,4-b)(1,4)benzothiazin-2(3H)-one), G-clamps such as a substituted phenoxazine cytidine (e.g. 9-(2-aminoethoxy)-H-pyrimido(5,4-(b) (1,4)benzoxazin-2(3H)-one), carbazole cytidine (2H-pyrimido(4,5-b)indol-2-one), pyridoindole cytidine (Hpyrido(3′,′:4,5)pyrrolo[2,3-d]pyrimidin-2-one).

As used herein, the term “sample” can refer to a composition comprising targets. Suitable samples for analysis by the disclosed methods, devices, and systems include cells, single cells, tissues, organs, or organisms.

As used herein, the term “sampling device” or “device” can refer to a device which may take a section of a sample and/or place the section on a substrate. A sample device can refer to, for example, a fluorescence activated cell sorting (FACS) machine, a cell sorter machine, a biopsy needle, a biopsy device, a tissue sectioning device, a microfluidic device, a blade grid, and/or a microtome.

As used herein, the term “solid support” can refer to discrete solid or semi-solid surfaces to which a plurality of stochastic barcodes may be attached. A solid support may encompass any type of solid, porous, or hollow sphere, ball, bearing, cylinder, or other similar configuration composed of plastic, ceramic, metal, or polymeric material (e.g., hydrogel) onto which a nucleic acid may be immobilized (e.g., covalently or non-covalently). A solid support may comprise a discrete particle that may be spherical (e.g., microspheres) or have a non-spherical or irregular shape, such as cubic, cuboid, pyramidal, cylindrical, conical, oblong, or disc-shaped, and the like. A plurality of solid supports spaced in an array may not comprise a substrate. A solid support may be used interchangeably with the term “bead.” As used herein, “solid support” and “substrate” can be used interchangeably.

As used here, the term “target” can refer to a composition which can be associated with a stochastic barcode. Exemplary suitable targets for analysis by the disclosed methods, devices, and systems include oligonucleotides, DNA, RNA, mRNA, microRNA, tRNA, and the like. Targets can be single or double stranded. In some embodiments targets can be proteins. In some embodiments targets are lipids. As used herein, “target” can be used interchangeably with “species”.

The term “reverse transcriptases” can refer to a group of enzymes having reverse transcriptase activity (i.e., that catalyze synthesis of DNA from an RNA template). In general, such enzymes include, but are not limited to, retroviral reverse transcriptase, retrotransposon reverse transcriptase, retroplasmid reverse transcriptases, retron reverse transcriptases, bacterial reverse transcriptases, group II intron-derived reverse transcriptase, and mutants, variants or derivatives thereof. Non-retroviral reverse transcriptases include non-LTR retrotransposon reverse transcriptases, retroplasmid reverse transcriptases, retron reverse transciptases, and group II intron reverse transcriptases. Examples of group II intron reverse transcriptases include the Lactococc s lactis Ll.LtrB intron reverse transcriptase, the Thermosynechococcus elongatus TeI4c intron reverse transcriptase, or the Geobacillus stearothermophilus GsI-IIC intron reverse transcriptase. Other classes of reverse transcriptases can include many classes of non-retroviral reverse transcriptases (i.e., retrons, group II introns, and diversity-generating retroelements among others).

Methods of Labeling a Target Nucleic Acid

This disclosure provides methods that allow for labeling a target nucleic acid with one or more molecular barcodes, for example, through intramolecular ligation. The end products of these methods are suitable for, for example, sequence identification, transcript counting, alternative splicing analysis, mutation screening, and full length mRNA sequencing in a high throughput manner without the use of long read sequencing. The methods disclosed herein can be used for associating a molecular barcode with a target nucleic acid, wherein the target nucleic acid is located at the terminus or internally in a nucleic acid molecule, e.g., an mRNA molecule or a cDNA molecule. For example, the target nucleic acid can be located at least 200 nucleotides (nt), at least 300 nt, at least 400 nt, at least 500 nt, at least 600 nt, at least 700 nt, at least 800 nt, at least 900 nt, at least 1,000 nt, at least 2,000 nt, at least 3,000 nt, at least 4,000 nt, at least 5,000 nt, or more, from either the 5′ end or the 3′ end of the nucleic acid molecule.

Without being bound by any particular theory, the target nucleic acid can be a variety of sequences that are suitable for molecular barcoding, for example, a coding sequence for a functional domain, a mutation site, a splicing junction, a coding region, an untranslated region, etc. In some embodiments, the target nucleic acid can be any part of a nucleic acid molecule. In some embodiments, the nucleic acid molecule can comprise a T cell receptor gene, an immunoglobulin gene, an MHC gene, a tumor suppressor gene, an oncogene, a transcription factor gene, a cell-surface gene, etc. In some embodiments, the target nucleic acid can comprise about 20 nt, about 30 nt, about 40 nt, about 50 nt, about 60 nt, about 70 nt, about 80 nt, about 90 nt, about 100 nt, about 200 nt, about 300 nt, about 400 nt, about 500 nt, or a range between any two of the above values.

Hybridizing an Oligonucleotide with a Nucleic Acid Molecule

In some embodiments, a nucleic acid molecule comprising a target nucleic acid is hybridized to an oligonucleotide comprising a molecular barcode. The oligonucleotide can be a variety of lengths, such as about 20 nt, about 30 nt, about 40 nt, about 50 nt, about 60 nt, about 70 nt, about 80 nt, about 90 nt, about 100 nt, about 200 nt, or more, or a range between any two of the above values. The molecular barcode can comprise, or be, a molecular label, a sample label, a cellular label, a universal label, or any combination thereof.

In some embodiments, the oligonucleotide can comprise a binding region that specifically binds to a binding site on the nucleic acid molecule. The binding site can be, or comprise, for example, a gene-specific sequence, a poly-A sequence, a 5′ sequence, a 3′ sequence, or a combination thereof. It would be appreciated that the binding site can be located at various distances from the target nucleic acid on the nucleic acid molecule. For example, on the nucleic acid molecule, the binding site can be located at least 20 nt, at least 50 nt, at least 100 nt, at least 200 nt, at least 300 nt, at least 400 nt, at least 500 nt, at least 600 nt, at least 700 nt, at least 800 nt, at least 900 nt, at least 1,000 nt, at least 2,000 nt, at least 3,000 nt, at least 4,000 nt, at least 5,000 nt, or more, from the target nucleic acid. In some embodiments, the binding site is located 20 nt, 50 nt, 100 nt, 200 nt, 300 nt, 400 nt, 500 nt, 600 nt, 700 nt, 800 nt, 900 nt, 1,000 nt, 2,000 nt, 3,000 nt, 4,000 nt, 5,000 nt, or a range between any two of these values, from the target nucleic acid on the nucleic acid molecule.

Extension

In some embodiments, the hybridized oligonucleotides can be extended using the nucleic acid molecule as a template to generate a new oligonucleotide comprising a molecular barcode. In some embodiments where the nucleic acid molecule is an RNA molecule, the oligonucleotide can be extended using reverse transcription. Reverse transcription of the associated RNA molecule may occur by the addition of a reverse transcriptase And cDNA molecules can be generated by the reverse transcription reactions. In some embodiments, a second strand DNA is generated using the cDNA molecules as a template. Second strand synthesis can be performed using a primer that is specific for the nucleic acid molecule. It would be appreciated that the primer preferably binds to the cDNA molecule at a location that is away from the target nucleic acid. In some embodiments, the primer can bind to the cDNA molecule at a location that is about 10 nt, about 20 nt, about 30 nt, about 40 nt, about 50 nt, about 100 nt, a range between any two of these values, or more away from the target nucleic acid.

Amplification of Extension Products

In some embodiments, the extension product, the cDNA from the reverse transcription step or the double-stranded DNA can be used as a template for amplification. One or more nucleic acid amplification reactions can be performed to create multiple copies of the molecular labeled nucleic acid molecules. In some embodiments, the amplification can be performed using a universal primer that binds to a binding site on the oligonucleotide.

Amplification can be performed using a primer that is specific for the nucleic acid molecule. It would be appreciated that the primer preferably binds to the extension product, the cDNA from the reverse transcription step or the double-stranded DNA at a location that is away from the target nucleic acid. In some embodiments, the primer can bind to the extension product (for example, the cDNA from the reverse transcription step or the double-stranded DNA) at a location that is about 10 nt, about 20 nt, about 30 nt, about 40 nt, about 50 nt, about 100 nt, a range between any two of these values, or more away from the target nucleic acid. In some embodiments, the extension product (for example, the cDNA from the reverse transcription step or the double-stranded DNA) can be ligated with an adaptor. In some embodiments, the adaptor can comprise a binding site for a second universal primer. The second universal primer can be used, for example, for the amplification of the extension product (e.g., the cDNA from the reverse transcription step or the double-stranded DNA).

The term “adaptor” can refer to a single stranded, partially double-stranded, or double-stranded, oligonucleotide of at least 5, 10, 15, 20 or 25 bases that can be attached to the end of a nucleic acid. Adaptor sequences can comprise, for example, priming sites, the complement of a priming site, and recognition sites for endonucleases, common sequences and promoters. The adaptor can be entirely or substantially double stranded. A double stranded adaptor can comprise two oligonucleotides that are at least partially complementary. The adaptor can be phosphorylated or unphosphorylated on one or both strands. The adaptor can have a double-stranded section and a single-stranded overhang section that is completely or partially complementary to an overhang (e.g., generated by a restriction enzyme, or a polymerase enzyme). The overhang in the adaptor can be, for example, 4 to 8 bases. For example, when DNA is digested with the restriction enzyme EcoRI, the resulting double stranded fragments are flanked at either end by the single stranded overhang 5′-AATT-3′, an adaptor that carries a single stranded overhang 5′-AATT-3′ can hybridize to the fragment through complementarity between the overhanging regions. This “sticky end” hybridization of the adaptor to the fragment facilitates ligation of the adaptor to the fragment; however, blunt ended ligation is also possible. Blunt ends can be converted to sticky ends using, for example, the exonuclease activity of the Klenow fragment. For example when DNA is digested with PvuII, the blunt ends can be converted to a two base pair overhang by incubating the fragments with Klenow in the presence of dTTP and dCTP. Overhangs can also be converted to blunt ends by filling in an overhang or removing an overhang.

Amplification may be performed in a multiplexed manner, wherein multiple nucleic acid sequences are amplified simultaneously. The amplification reactions may comprise amplifying at least a portion of a sample label, if present. The amplification reactions may comprise amplifying at least a portion of the cellular and/or molecular label. The amplification reactions may comprise amplifying at least a portion of a sample tag, a cellular label, a spatial label, a molecular label, a target nucleic acid, or a combination thereof. The amplification reactions may comprise amplifying at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, or 100% of the plurality of nucleic acids. The amplification reactions may comprise amplifying 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, 100%, or a range of any two of these values, of the plurality of nucleic acids.

In some embodiments, amplification is performed using a polymerase chain reaction (PCR). As used herein, PCR refers to a reaction for the in vitro amplification of specific DNA sequences by the simultaneous primer extension of complementary strands of DNA. As used herein, PCR encompasses derivative forms of the reaction, including but not limited to, RT-PCR, real-time PCR, nested PCR, quantitative PCR, multiplexed PCR, digital PCR, and assembly PCR.

Amplification of the labeled nucleic acids can comprise non-PCR based methods. Examples of non-PCR based methods include, but are not limited to, multiple displacement amplification (MDA), transcription-mediated amplification (TMA), whole transcriptome amplification (WTA), whole genome amplification (WGA), nucleic acid sequence-based amplification (NASBA), strand displacement amplification (SDA), real-time SDA, rolling circle amplification, or circle-to-circle amplification. Other non-PCR-based amplification methods include multiple cycles of DNA-dependent RNA polymerase-driven RNA transcription amplification or RNA-directed DNA synthesis and transcription to amplify DNA or RNA targets, a ligase chain reaction (LCR), and a Qβ replicase (Qβ) method, use of palindromic probes, strand displacement amplification, oligonucleotide-driven amplification using a restriction endonuclease, an amplification method in which a primer is hybridized to a nucleic acid sequence and the resulting duplex is cleaved prior to the extension reaction and amplification, strand displacement amplification using a nucleic acid polymerase lacking 5′ exonuclease activity, rolling circle amplification, and ramification extension amplification (RAM). In some instances, the amplification may not produce circularized transcripts.

Amplification may comprise use of one or more non-natural nucleotides. Non-natural nucleotides may comprise photolabile or triggerable nucleotides. Examples of non-natural nucleotides can include, but are not limited to, peptide nucleic acid (PNA), morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Non-natural nucleotides may be added to one or more cycles of an amplification reaction. The addition of the non-natural nucleotides may be used to identify products as specific cycles or time points in the amplification reaction.

Conducting the one or more amplification reactions may comprise the use of one or more primers. The one or more primers may comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 or more nucleotides. The one or more primers may comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 or more nucleotides. The one or more primers may comprise less than 12-15 nucleotides. The one or more primers may anneal to at least a portion of the molecular barcoded nucleic acid molecules. The one or more primers may anneal to the 3′ end or 5′ end of the molecular barcoded nucleic acid molecules. The one or more primers may anneal to an internal region of the molecular barcoded nucleic acid molecules. The internal region may be at least about 50, 100, 150, 200, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 650, 700, 750, 800, 850, 900 or 1000 nucleotides from the 3′ ends of the molecular labeled reference gene(s) and/or spike-in RNA. The one or more primers may comprise a fixed panel of primers. The one or more primers may comprise at least one or more customized primers. The one or more primers may comprise at least one or more control primers. The one or more primers may comprise at least one or more gene-specific primers.

The one or more primers may comprise any universal primer of the disclosure. The universal primer may anneal to a universal primer binding site. The one or more customized primers may anneal to a sample label, a spatial label, a cellular label, a molecular label, a target, or any combination thereof. The one or more primers can, in some embodiments, comprise one or more of a universal primer and a customized primer. The customized primer may be designed to specifically amplify the molecular barcoded nucleic acid molecules. The one or more primers may comprise at least 96 or more customized primers. The one or more primers may comprise at least 960 or more customized primers. The one or more primers may comprise at least 9600 or more customized primers. The one or more customized primers may anneal to two or more different barcoded nucleic acid molecules. The two or more different barcoded nucleic acid molecules may correspond to one or more genes.

Circularization of Extension/Amplification Products

In the methods described herein, he extension product (e.g., the cDNA from the reverse transcription step, the double-stranded DNA, etc.) or the amplification product thereof can be circularized through, e.g., intramolecular ligation. In some embodiments, the intramolecular ligation can be performed on a single-stranded DNA. In some embodiments, the intramolecular ligation can be performed on a double-stranded DNA. In some embodiments, the intramolecular ligation is performed on the cDNA obtained from the reverse transcription step, or complement thereof. In some embodiments, the intramolecular ligation is performed on amplicons of the cDNA or complement thereof. In some embodiments, the intramolecular ligation is performed on one of the strands of the amplicons of the cDNA.

As a result of the intramolecular ligation, a circularized nucleic acid molecule (single-stranded or double-stranded) is produced. Without being bound by any particular theory, in the circularized nucleic acid molecule, the molecular barcode, or part thereof, can be in close proximity to the target nucleic acid. For example, in the circularized nucleic acid molecule, the molecular barcode, or part thereof, can be at most 500 nt, at most 400 nt, at most 300 nt, at most 200 nt, at most 100 nt, at most 90 nt, at most 80 nt, at most 70 nt, at most 60 nt, at most 50 nt, at most 40 nt, at most 30 nt, at most 20 nt, at most 10 nt, or less, away from the target nucleic acid. In some embodiments, in the circularized nucleic acid molecule, the molecular barcode, or part thereof, is, or is about, 1000 nt, 750 nt, 500 nt, 400 nt, 300 nt, 200 nt, 100 nt, 90 nt, 80 nt, 70 nt, 60 nt, 50 nt, 40 nt, 30 nt, 20 nt, 10 nt, or a range between any two of these values, away from the target nucleic acid.

Amplifying the Circularization Product

As described herein, the circularization product can be amplified to produce a plurality of amplicons comprising the molecular barcode in close proximity to the target nucleic acid. In some embodiments, the plurality amplicons comprises, or are, linear amplicons.

Amplification can be performed, for example, using a primer that is specific for the nucleic acid molecule. It would be appreciated that the primer preferably binds to the circularization product at a location that is away from the target nucleic acid. In some embodiments, the primer can bind to the circularization product at a location that is about 10 nt, about 20 nt, about 30 nt, about 40 nt, about 50 nt, about 100 nt, or a range between any two of these values, away from the target nucleic acid. In some embodiments, the primer can bind to the circularization product at a location that is at least, or at least about, 10 nt, 20 nt, 30 nt, 40 nt, 50 nt, 100 nt, or more, away from the target nucleic acid. In some embodiments, the primer can bind to the circularization product at a location that is at most, or at most about, 10 nt, 20 nt, 30 nt, 40 nt, 50 nt, 100 nt, or more, away from the target nucleic acid.

Amplification may be performed in a multiplexed manner, wherein multiple nucleic acid sequences are amplified simultaneously. The amplification reactions may comprise amplifying at least a portion of a sample label, if present. The amplification reactions may comprise amplifying at least a portion of the cellular and/or molecular label. The amplification reactions may comprise amplifying at least a portion of a sample tag, a cellular label, a spatial label, a molecular label, a target nucleic acid, or a combination thereof. The amplification reactions may comprise amplifying at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, or 100% of the circularization products. The amplification reactions may comprise amplifying 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, 100%, or a range between any two of these values, of the circularization products.

In some embodiments, the amplification is performed using a polymerase chain reaction (PCR). As used herein, PCR may refer to a reaction for the in vitro amplification of specific DNA sequences by the simultaneous primer extension of complementary strands of DNA. As used herein, PCR may encompass derivative forms of the reaction, including but not limited to, RT-PCR, real-time PCR, nested PCR, quantitative PCR, multiplexed PCR, digital PCR, and assembly PCR.

Amplification of the labeled nucleic acids can comprise non-PCR based methods. Examples of non-PCR based methods include, but are not limited to, multiple displacement amplification (MDA), transcription-mediated amplification (TMA), whole transcriptome amplification (WTA), whole genome amplification (WGA), nucleic acid sequence-based amplification (NASBA), strand displacement amplification (SDA), real-time SDA, rolling circle amplification, or circle-to-circle amplification. Other non-PCR-based amplification methods include multiple cycles of DNA-dependent RNA polymerase-driven RNA transcription amplification or RNA-directed DNA synthesis and transcription to amplify DNA or RNA targets, a ligase chain reaction (LCR), and a Qβ replicase (Qβ) method, use of palindromic probes, strand displacement amplification, oligonucleotide-driven amplification using a restriction endonuclease, an amplification method in which a primer is hybridized to a nucleic acid sequence and the resulting duplex is cleaved prior to the extension reaction and amplification, strand displacement amplification using a nucleic acid polymerase lacking 5′ exonuclease activity, rolling circle amplification, and ramification extension amplification (RAM). In some instances, the amplification may not produce circularized transcripts.

Amplification may comprise use of one or more non-natural nucleotides. Non-natural nucleotides may comprise photolabile or triggerable nucleotides. Examples of non-natural nucleotides can include, but are not limited to, peptide nucleic acid (PNA), morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Non-natural nucleotides may be added to one or more cycles of an amplification reaction. The addition of the non-natural nucleotides may be used to identify products as specific cycles or time points in the amplification reaction.

Conducting the one or more amplification reactions may comprise the use of one or more primers. The one or more primers may comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 or more nucleotides. The one or more primers may comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 or more nucleotides. The one or more primers may comprise less than 12-15 nucleotides. The one or more primers may anneal to at least a portion of the molecular barcoded nucleic acid molecules. The one or more primers may anneal to an internal region of the circularization product. The one or more primers may comprise at least one or more customized primers. The one or more primers may comprise at least one or more control primers. The one or more primers may comprise at least one or more gene-specific primers.

The one or more primers may comprise any universal primer of the disclosure. The universal primer may anneal to a universal primer binding site. The one or more customized primers may anneal to a first sample label, a second sample label, a spatial label, a cellular label, a molecular label, a target, or any combination thereof. The one or more primers may comprise a universal primer and a customized primer. The customized primer may be designed to amplify the circularization product. The one or more primers may comprise at least 96 or more customized primers. The one or more primers may comprise at least 960 or more customized primers. The one or more primers may comprise at least 9600 or more customized primers. The one or more customized primers may anneal to two or more different circularization product. The two or more different circularization product may correspond to one or more genes.

Any amplification scheme can be used in the methods of the present disclosure. For example, in one embodiments, the first round PCR can amplify molecules (e.g., attached to the bead) using a gene specific primer and a primer against the universal Illumina sequencing primer 1 sequence. The second round of PCR can amplify the first PCR products using a nested gene specific primer flanked by Illumina sequencing primer 2 sequence, and a primer against the universal Illumina sequencing primer 1 sequence. The third round of PCR adds P5 and P7 and sample index to turn PCR products into an Illumina sequencing library. Sequencing using 150 bp×2 sequencing can reveal the cell label and molecular index on read 1, the gene on read 2, and the sample index on index 1 read.

Amplification can be performed in one or more rounds. In some instances there are multiple rounds of amplification. Amplification can comprise two or more rounds of amplification. The first amplification can be an extension to generate the gene specific region. The second amplification can occur when a sample nucleic hybridizes to the newly generated strand.

Sequencing

The amplicons comprising the molecular barcode in close proximity to the target nucleic acid (for example, the linear amplicons) can be subject to sequencing reactions to determine the target nucleic acid sequence, the molecular barcode or part thereof, or both. Any suitable sequencing method known in the art can be used, preferably high-throughput approaches. For example, cyclic array sequencing using platforms such as Roche 454, Illumina Solexa, ABI-SOLiD, ION Torrent, Complete Genomics, Pacific Bioscience, Helicos, or the Polonator platform, may also be utilized. Sequencing may comprise MiSeq sequencing. Sequencing may comprise HiSeq sequencing.

In some embodiments, sequencing can comprise sequencing at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 or more nucleotides or base pairs of the labeled nucleic acid and/or molecular barcode. In some embodiments, sequencing can comprise sequencing at most about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 or more nucleotides or base pairs of the labeled nucleic acid and/or molecular barcode. In some embodiments, sequencing can comprise sequencing at least about 200, 300, 400, 500, 600, 700, 800, 900, 1,000 or more nucleotides or base pairs of the labeled nucleic acid and/or molecular barcode. In some embodiments, sequencing can comprise sequencing at most about 200, 300, 400, 500, 600, 700, 800, 900, 1,000 or more nucleotides or base pairs of the labeled nucleic acid and/or stochastic barcode. In some embodiments, sequencing can comprise sequencing at least about 1,500; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; 8,000; 9,000; or 10,000 or more nucleotides or base pairs of the labeled nucleic acid and/or stochastic barcode. In some embodiments, sequencing can comprise sequencing at most about 1,500; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; 8,000; 9,000; or 10,000 or more nucleotides or base pairs of the labeled nucleic acid and/or molecular barcode.

In some embodiments, sequencing can comprise at least about 200, 300, 400, 500, 600, 700, 800, 900, 1,000 or more sequencing reads per run. In some embodiments, sequencing can comprise at most about 200, 300, 400, 500, 600, 700, 800, 900, 1,000 or more sequencing reads per run. In some embodiments, sequencing comprises sequencing at least about 1,500; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; 8,000; 9,000; or 10,000 or more sequencing reads per run. In some embodiments, sequencing comprises sequencing at most about 1,500; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; 8,000; 9,000; or 10,000 or more sequencing reads per run. In some embodiments, sequencing can comprise sequencing at least 10, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950 or 1000 or more millions of sequencing reads per run. In some embodiments, sequencing can comprise sequencing at most 10, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950 or 1000 or more millions of sequencing reads per run. In some embodiments, sequencing can comprise sequencing at least 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 2000, 3000, 4000, or 5000 or more millions of sequencing reads in total. In some embodiments, sequencing can comprise sequencing at most 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 2000, 3000, 4000, or 5000 or more millions of sequencing reads in total. In some embodiments, sequencing can comprise less than or equal to about 1,600,000,000 sequencing reads per run. In some embodiments, sequencing can comprise less than or equal to about 200,000,000 reads per run.

An exemplary method for labeling a target nucleic acid is illustrated in FIG. 1. As shown, an mRNA molecule 130 can be hybridized to an oligonucleotide 100, which can comprise a binding site for a universal primer 105, a sample label 110, a molecular label 115, a binding site for a second universal primer 120, and an oligo-dT 125. After a reverse transcription step to generate a cDNA 150, a primer 140 that specifically binds to the cDNA at a location beyond the target nucleic acid 145 and a universal primer that binds to the binding site 105 are used to amplify the cDNA 150. The amplification product is denatured to single-stranded DNA molecules which are circularized by intramolecular ligation to produce a circularized DNA molecule 160. Another PCR amplification reaction is conducted using a second universal primer 165 and a primer 170 that binds to the circularized DNA molecule 160 at a location beyond the target nucleic acid 145, to produce a linearized amplicon 180. The linearized amplicon 180 can be amplified using primers 190 and 195 which comprise binding sites for sequencing primers. In some embodiments, the target nucleic acid 145 can be a CDR3 sequence in a T cell receptor gene.

Methods of Generating Sequencing Library

Some embodiments disclosed herein provide methods of generating a sequencing library for a target nucleic acid molecule from a sample. In some embodiments, the target nucleic acid molecule is a DNA, a cDNA, a genomic DNA, an mRNA, or a combination thereof. In some embodiments, the target nucleic acid molecule can comprise unknown sequences. In some embodiments, the target nucleic acid molecule can be at least 1,000 nt, at least 2,000 nt, at least 3,000 nt, at least 4,000 nt, at least 5,000 nt, at least 6,000 nt, at least 7,000 nt, at least 8,000 nt, at least 9,000 nt, at least 10,000 nt, at least 20,000 nt, at least 50,000 nt, at least 100,000 nt, or more, in length.

Hybridizing an Oligonucleotide with a Target Nucleic Acid Molecule

In some embodiments, a target nucleic acid molecule comprising a target nucleic acid is hybridized to an oligonucleotide comprising a molecular barcode. The oligonucleotide can be a variety of lengths, such as about 20 nt, about 30 nt, about 40 nt, about 50 nt, about 60 nt, about 70 nt, about 80 nt, about 90 nt, about 100 nt, about 200 nt, or more, or a range between any two of the above values. The molecular barcode can comprise a molecular label, a sample label, a cellular label, a universal label, or any combination thereof. In some embodiments, the oligonucleotide can comprise a restriction site.

In some embodiments, the oligonucleotide can comprise a binding region that specifically binds to a binding site on the target nucleic acid molecule. The binding site can be, or comprise, a gene-specific sequence, a poly-A sequence, a 5′ sequence, a 3′ sequence, or a combination thereof.

Extension

In some embodiments, the hybridized oligonucleotides can be extended using the target nucleic acid molecule as a template. In some embodiments where the nucleic acid molecule is an RNA molecule, the oligonucleotide can be extended using reverse transcription. Reverse transcription of the associated RNA molecule may occur by the addition of a reverse transcriptase. cDNA molecules are generated by the reverse transcription reactions. In some embodiments, a second strand DNA is generated using the cDNA molecules as a template. Second strand synthesis can be performed using a primer that is specific for the target nucleic acid molecule.

Amplification of Extension Products

In some embodiments, the extension product, the cDNA from the reverse transcription step or the double-stranded DNA can be used as a template for amplification. One or more nucleic acid amplification reactions may be performed to create multiple copies of the molecular labeled target nucleic acid molecules. In some embodiments, the amplification can be performed using a universal primer that binds to a binding site on the oligonucleotide.

Amplification can be performed using a primer that is specific for the target nucleic acid molecule. In some embodiments, the extension product, the cDNA from the reverse transcription step or the double-stranded DNA can be ligated with an adaptor. In some embodiments, the adaptor can comprise a binding site for a second universal primer. The second universal primer can be used for the amplification of the extension product, the cDNA from the reverse transcription step or the double-stranded DNA.

Adaptor sequences can be synthesized using for example, priming sites, the complement of a priming site, and recognition sites for endonucleases, common sequences and promoters. The adaptor can be entirely or substantially double stranded. A double stranded adaptor can comprise two oligonucleotides that are at least partially complementary. The adaptor can be phosphorylated or unphosphorylated on one or both strands. The adaptor can have a double stranded section and a single stranded overhang section that is completely or partially complementary to an overhang (e.g., generated by a restriction enzyme, or a polymerase enzyme). The overhang in the adaptor can be, for example, 4 to 8 bases. For example, when DNA is digested with the restriction enzyme EcoRI, the resulting double stranded fragments are flanked at either end by the single stranded overhang 5′-AATT-3′, an adaptor that carries a single stranded overhang 5′-AATT-3′ can hybridize to the fragment through complementarity between the overhanging regions. This “sticky end” hybridization of the adaptor to the fragment facilitates ligation of the adaptor to the fragment; however, blunt ended ligation is also possible. Blunt ends can be converted to sticky ends using, for example, the exonuclease activity of the Klenow fragment. For example when DNA is digested with PvuII the blunt ends can be converted to a two base pair overhang by incubating the fragments with Klenow in the presence of dTTP and dCTP. Overhangs can also be converted to blunt ends by filling in an overhang or removing an overhang.

Amplification may be performed in a multiplexed manner, wherein multiple nucleic acid sequences are amplified simultaneously. The amplification reactions may comprise amplifying at least a portion of a sample label, if present. The amplification reactions may comprise amplifying at least a portion of the cellular and/or molecular label. The amplification reactions may comprise amplifying at least a portion of a sample tag, a cellular label, a spatial label, a molecular label, a target nucleic acid, or a combination thereof. The amplification reactions may comprise amplifying at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, or 100% of the plurality of nucleic acids.

In some embodiments, amplification may be performed using a polymerase chain reaction (PCR). As used herein, PCR may refer to a reaction for the in vitro amplification of specific DNA sequences by the simultaneous primer extension of complementary strands of DNA. As used herein, PCR may encompass derivative forms of the reaction, including but not limited to, RT-PCR, real-time PCR, nested PCR, quantitative PCR, multiplexed PCR, digital PCR, and assembly PCR.

Amplification of the labeled nucleic acids can comprise non-PCR based methods. Examples of non-PCR based methods include, but are not limited to, multiple displacement amplification (MDA), transcription-mediated amplification (TMA), whole transcriptome amplification (WTA), whole genome amplification (WGA), nucleic acid sequence-based amplification (NASBA), strand displacement amplification (SDA), real-time SDA, rolling circle amplification, or circle-to-circle amplification. Other non-PCR-based amplification methods include multiple cycles of DNA-dependent RNA polymerase-driven RNA transcription amplification or RNA-directed DNA synthesis and transcription to amplify DNA or RNA targets, a ligase chain reaction (LCR), and a Qβ replicase (Qβ) method, use of palindromic probes, strand displacement amplification, oligonucleotide-driven amplification using a restriction endonuclease, an amplification method in which a primer is hybridized to a nucleic acid sequence and the resulting duplex is cleaved prior to the extension reaction and amplification, strand displacement amplification using a nucleic acid polymerase lacking 5′ exonuclease activity, rolling circle amplification, and ramification extension amplification (RAM). In some instances, the amplification may not produce circularized transcripts.

Amplification may comprise use of one or more non-natural nucleotides. Non-natural nucleotides may comprise photolabile or triggerable nucleotides. Examples of non-natural nucleotides can include, but are not limited to, peptide nucleic acid (PNA), morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Non-natural nucleotides may be added to one or more cycles of an amplification reaction. The addition of the non-natural nucleotides may be used to identify products as specific cycles or time points in the amplification reaction.

Conducting the one or more amplification reactions may comprise the use of one or more primers. The one or more primers may comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 or more nucleotides. The one or more primers may comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 or more nucleotides. The one or more primers may comprise less than 12-15 nucleotides. The one or more primers may anneal to at least a portion of the molecular barcoded nucleic acid molecules. The one or more primers may anneal to the 3′ end or 5′ end of the molecular barcoded nucleic acid molecules. The one or more primers may anneal to an internal region of the molecular barcoded nucleic acid molecules. The internal region may be at least about 50, 100, 150, 200, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 650, 700, 750, 800, 850, 900 or 1000 nucleotides from the 3′ ends of the molecular labeled reference gene(s) and/or spike-in RNA. The one or more primers may comprise a fixed panel of primers. The one or more primers may comprise at least one or more customized primers. The one or more primers may comprise at least one or more control primers. The one or more primers may comprise at least one or more gene-specific primers.

The one or more primers may comprise any universal primer of the disclosure. The universal primer may anneal to a universal primer binding site. The one or more customized primers may anneal to a first sample label, a second sample label, a spatial label, a cellular label, a molecular label, a target, or any combination thereof. The one or more primers may comprise a universal primer and a customized primer. The customized primer may be designed to amplify the molecular barcoded nucleic acid molecules. The one or more primers may comprise at least 96 or more customized primers. The one or more primers may comprise at least 960 or more customized primers. The one or more primers may comprise at least 9600 or more customized primers. The one or more customized primers may anneal to two or more different barcoded nucleic acid molecules. The two or more different barcoded nucleic acid molecules may correspond to one or more genes.

Fragmentation of Amplification Products

The amplification products can be fragmented to produce a plurality of nucleic acid fragments. In some embodiments, the fragmentation can be partial fragmentation, so that fragments of the target nucleic acid molecule can have different lengths. Fragmentation can be conducted by, for example, sonication, restriction enzyme digestion, or any other suitable methods. In some embodiments, two or more of the plurality of nucleic acid fragments have the same 5′ terminus but different 3′ terminus. In some embodiments, two or more of the plurality of nucleic acid fragments have the same 3′ terminus but different 5′ terminus. In some embodiments, each of the plurality of nucleic acid fragments has a length between 50 nt to 10,000 nt. In some embodiments, the plurality of nucleic acid fragments comprises at least 2 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 10 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 100 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 1,000 nucleic acid fragments. In some embodiments, the plurality of nucleic acid fragments comprises at least 10,000 nucleic acid fragments. In some embodiments, the fragmenting comprises restriction digestion of the first plurality of amplicons. In some embodiments, at least 50% of the plurality of nucleic acid fragments comprises different length. In some embodiments, at least 80% of the plurality of nucleic acid fragments comprises different length. In some embodiments, at least 90% of the plurality of nucleic acid fragments comprises different length.

In some embodiments, the fragments can be subject to purification (e.g., washing) to remove fragments that do not comprise the molecular barcode. For example, the fragments can be immobilized to a solid support through the oligonucleotide, and unbound fragments can be removed.

Circularization of Fragments

In some embodiments, the fragments of the target nucleic acid molecule can be circularized through, e.g., intramolecular ligation. In some embodiments, the intramolecular ligation can be performed on a single-stranded DNA. In some embodiments, the intramolecular ligation can be performed on a double-stranded DNA.

As a result of the intramolecular ligation, circularized nucleic acid molecules having various sizes are produced.

Amplifying the Circularization Product

As described herein, the circularization product can be amplified to produce a plurality of amplicons comprising the molecular barcode associated with fragments of the target nucleic acid molecule. In some embodiments, the plurality amplicons comprises, or is, linear amplicons.

Amplification can be performed, for example, using a universal primer that binds to a binding site on the oligonucleotide. In some embodiments, two universal primers that bind to the oligonucleotide in opposite directions can be used to linearize the circulated product.

Amplification may be performed in a multiplexed manner, wherein multiple nucleic acid sequences are amplified simultaneously. The amplification reactions may comprise amplifying at least a portion of a sample label, if present. The amplification reactions may comprise amplifying at least a portion of the cellular and/or molecular label. The amplification reactions may comprise amplifying at least a portion of a sample tag, a cellular label, a spatial label, a molecular label, a target nucleic acid, or a combination thereof. The amplification reactions may comprise amplifying at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, or 100% of the circularization products. The amplification reactions may comprise amplifying 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, 100%, or a range between any two of these values, of the circularization products.

In some embodiments, the amplification is performed using a polymerase chain reaction (PCR). As used herein, PCR may refer to a reaction for the in vitro amplification of specific DNA sequences by the simultaneous primer extension of complementary strands of DNA. As used herein, PCR may encompass derivative forms of the reaction, including but not limited to, RT-PCR, real-time PCR, nested PCR, quantitative PCR, multiplexed PCR, digital PCR, and assembly PCR.

Amplification of the labeled nucleic acids can comprise non-PCR based methods. Examples of non-PCR based methods include, but are not limited to, multiple displacement amplification (MDA), transcription-mediated amplification (TMA), whole transcriptome amplification (WTA), whole genome amplification (WGA), nucleic acid sequence-based amplification (NASBA), strand displacement amplification (SDA), real-time SDA, rolling circle amplification, or circle-to-circle amplification. Other non-PCR-based amplification methods include multiple cycles of DNA-dependent RNA polymerase-driven RNA transcription amplification or RNA-directed DNA synthesis and transcription to amplify DNA or RNA targets, a ligase chain reaction (LCR), and a Qβ replicase (Qβ) method, use of palindromic probes, strand displacement amplification, oligonucleotide-driven amplification using a restriction endonuclease, an amplification method in which a primer is hybridized to a nucleic acid sequence and the resulting duplex is cleaved prior to the extension reaction and amplification, strand displacement amplification using a nucleic acid polymerase lacking 5′ exonuclease activity, rolling circle amplification, and ramification extension amplification (RAM). In some instances, the amplification may not produce circularized transcripts.

Amplification may comprise use of one or more non-natural nucleotides. Non-natural nucleotides may comprise photolabile or triggerable nucleotides. Examples of non-natural nucleotides can include, but are not limited to, peptide nucleic acid (PNA), morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Non-natural nucleotides may be added to one or more cycles of an amplification reaction. The addition of the non-natural nucleotides may be used to identify products as specific cycles or time points in the amplification reaction.

Conducting the one or more amplification reactions may comprise the use of one or more primers. The one or more primers may comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 or more nucleotides. The one or more primers may comprise at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 or more nucleotides. The one or more primers may comprise less than 12-15 nucleotides. The one or more primers may anneal to at least a portion of the molecular barcoded nucleic acid molecules. The one or more primers may anneal to an internal region of the circularization product. The one or more primers may comprise at least one or more customized primers. The one or more primers may comprise at least one or more control primers. The one or more primers may comprise at least one or more gene-specific primers.

The one or more primers may comprise any universal primer of the disclosure. The universal primer may anneal to a universal primer binding site. The one or more customized primers may anneal to a first sample label, a second sample label, a spatial label, a cellular label, a molecular label, a target, or any combination thereof. The one or more primers may comprise a universal primer and a customized primer. The customized primer may be designed to amplify the circularization product. The one or more primers may comprise at least 96 or more customized primers. The one or more primers may comprise at least 960 or more customized primers. The one or more primers may comprise at least 9600 or more customized primers. The one or more customized primers may anneal to two or more different circularization product. The two or more different circularization product may correspond to one or more genes.

Any amplification scheme can be used in the methods of the present disclosure. For example, in one scheme, the first round PCR can amplify molecules (e.g., attached to the bead) using a gene specific primer and a primer against the universal Illumina sequencing primer 1 sequence. The second round of PCR can amplify the first PCR products using a nested gene specific primer flanked by Illumina sequencing primer 2 sequence, and a primer against the universal Illumina sequencing primer 1 sequence. The third round of PCR adds P5 and P7 and sample index to turn PCR products into an Illumina sequencing library. Sequencing using 150 bp×2 sequencing can reveal the cell label and molecular index on read 1, the gene on read 2, and the sample index on index 1 read.

Amplification can be performed in one or more rounds. In some instances there are multiple rounds of amplification. Amplification can comprise two or more rounds of amplification. The first amplification can be an extension to generate the gene specific region. The second amplification can occur when a sample nucleic hybridizes to the newly generated strand.

Sequencing

The amplicons comprising the molecular barcode associated with fragments of the target nucleic acid molecule (for example, the linear amplicons) can be subject to sequencing reactions to determine the target nucleic acid sequence, the molecular barcode or part thereof, or both. Any suitable sequencing method known in the art can be used, preferably high-throughput approaches. For example, cyclic array sequencing using platforms such as Roche 454, Illumina Solexa, ABI-SOLiD, ION Torrent, Complete Genomics, Pacific Bioscience, Helicos, or the Polonator platform, may also be utilized. Sequencing may comprise MiSeq sequencing. Sequencing may comprise HiSeq sequencing.

In some embodiments, sequencing can comprise sequencing at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 or more nucleotides or base pairs of the labeled nucleic acid and/or molecular barcode. In some embodiments, sequencing can comprise sequencing at most about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 or more nucleotides or base pairs of the labeled nucleic acid and/or molecular barcode. In some embodiments, sequencing can comprise sequencing at least about 200, 300, 400, 500, 600, 700, 800, 900, 1,000 or more nucleotides or base pairs of the labeled nucleic acid and/or molecular barcode. In some embodiments, sequencing can comprise sequencing at most about 200, 300, 400, 500, 600, 700, 800, 900, 1,000 or more nucleotides or base pairs of the labeled nucleic acid and/or stochastic barcode. In some embodiments, sequencing can comprise sequencing at least about 1,500; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; 8,000; 9,000; or 10,000 or more nucleotides or base pairs of the labeled nucleic acid and/or stochastic barcode. In some embodiments, sequencing can comprise sequencing at most about 1,500; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; 8,000; 9,000; or 10,000 or more nucleotides or base pairs of the labeled nucleic acid and/or molecular barcode.

In some embodiments, sequencing can comprise at least about 200, 300, 400, 500, 600, 700, 800, 900, 1,000 or more sequencing reads per run. In some embodiments, sequencing can comprise at most about 200, 300, 400, 500, 600, 700, 800, 900, 1,000 or more sequencing reads per run. In some embodiments, sequencing comprises sequencing at least about 1,500; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; 8,000; 9,000; or 10,000 or more sequencing reads per run. In some embodiments, sequencing comprises sequencing at most about 1,500; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; 8,000; 9,000; or 10,000 or more sequencing reads per run. In some embodiments, sequencing can comprise sequencing at least 10, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950 or 1000 or more millions of sequencing reads per run. In some embodiments, sequencing can comprise sequencing at most 10, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950 or 1000 or more millions of sequencing reads per run. In some embodiments, sequencing can comprise sequencing at least 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 2000, 3000, 4000, or 5000 or more millions of sequencing reads in total. In some embodiments, sequencing can comprise sequencing at most 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 2000, 3000, 4000, or 5000 or more millions of sequencing reads in total. In some embodiments, sequencing can comprise less than or equal to about 1,600,000,000 sequencing reads per run. In some embodiments, sequencing can comprise less than or equal to about 200,000,000 reads per run.

FIG. 2 shows a schematic illustration of an exemplary method to produce a sequencing library of a target nucleic acid molecule. In a first step, a cDNA is produced by reverse transcription of an mRNA molecule using an oligonucleotide 205 comprising a restriction site, a binding site for a universal primer 215, a sample label, a molecular label, a binding site for a second universal primer 235, a binding site for a third universal primer 240, and a poly dT. A adaptor 210 having a binding site for primer 220 is ligated to the cDNA. Universal primers 215 and 220 are used to amplify the cDNA to produce a plurality of amplicons. The plurality of amplicons is partially digested to produce fragments of varying sizes, which are ligated to produce a plurality of circularized products 230 having different sizes. Inverse PCR using universal primers 235 and 240 is used to produce a plurality of linearized amplicons 245. PCR reaction using universal primer 235 and random primer 250 is used to generate a second plurality of amplicons 260. Universal primers 265 and 270 are used to amplify the second plurality of amplicons 260 to generate a sequencing library.

Compositions for Generating Sequencing Library

Some embodiments disclosed herein provide composition for generating a sequencing library for a plurality of nucleic acid molecules of a sample. In some embodiments, the compositions can comprise a plurality of oligonucleotides, wherein each of the plurality of oligonucleotides comprises from 5′ to 3′: a molecular label, a sample label, a binding site for a sequencing primer and a target-specific region that specifically binds to a nucleic acid molecule. In some embodiments, the binding site for the sequencing primer is oriented in the opposite direction of the oligonucleotide. In some embodiments, each of the plurality of oligonucleotides comprises a restriction enzyme recognition site 5′ to the molecular label. In some embodiments, each of the plurality of oligonucleotides comprises a binding site for a universal primer 5′ to the molecular label. In some embodiments, the oligonucleotide can further comprise a binding site for a second universal primer 3′ to the binding site for the sequencing primer, and oriented in the opposite direction of the binding site for the sequencing primer. An exemplary oligonucleotide has the following sequence: 3′ VTTTTTTTTTTTTTTTTTTGCTGCGAGAAGGCTAGASSSSSSSSMMMMMMMMCGCTAGCGGTTACAGGAGGTCTGGAGGACATTGGCGAT 5′ (SEQ ID NO:1), wherein the “V” represents A, G or C, the “SSSSSSSS” represents the sequence for the sample label, the “MMMMMMMM” represents the sequence for the molecular label, the “CGCTAGCG” is an AsiSI restriction site. Another exemplary oligonucleotide has the following sequence: 3′ TTTTTTTTTTTTTTTTTTTGCTGCGAGAAGGCTAGASSSSSSSSMMMMMMMMCGCTAGCGGTTACAGGAGGTCTGGAGGACATTGGCGAT 5′ (SEQ ID NO:2), wherein the “SSSSSSSS” represents the sequence for the sample label, the “MMMMMMMM” represents the sequence for the molecular label, the “CGCTAGCG” is an AsiSI restriction site.

Kits

Some embodiments disclosed herein provide kits for generating a sequencing library for a plurality of nucleic acid molecules of a sample, comprising a plurality of oligonucleotides as disclosed herein, and an enzyme. In some embodiments, each of the plurality of oligonucleotides comprises from 5′ to 3′: a molecular label, a sample label, a binding site for a sequencing primer and a target-specific region that specifically binds to a nucleic acid molecule. In some embodiments, the binding site for the sequencing primer is oriented in the opposite direction of the oligonucleotide. In some embodiments, each of the plurality of oligonucleotides comprises a restriction enzyme recognition site 5′ to the molecular label. In some embodiments, each of the plurality of oligonucleotides comprises a binding site for a universal primer 5′ to the molecular label. In some embodiments, the oligonucleotide can further comprise a binding site for a second universal primer 3′ to the binding site for the sequencing primer, and oriented in the opposite direction of the binding site for the sequencing primer. In some embodiments, enzyme is selected from the group consisting of a ligase, a restriction enzyme, a DNA polymerase, a reverse transcriptase, an RNase, or any combination thereof.

The kit can, in some embodiments, comprise one or more substrates (e.g., microwell array, Pixel device), either as a free-standing substrate (or chip) comprising one or more microwell arrays, or packaged within one or more flow-cells or cartridges. The kits can comprise one or more solid support suspensions, wherein the individual solid supports within a suspension comprise a plurality of attached stochastic barcodes of the disclosure. The kits can comprise stochastic barcodes that may not be attached to a solid support. In some embodiments, the kit may further comprise a mechanical fixture for mounting a free-standing substrate in order to create reaction wells that facilitate the pipetting of samples and reagents into the substrate. The kit may further comprise reagents, e.g. lysis buffers, rinse buffers, or hybridization buffers, for performing the stochastic barcoding assay. The kit may further comprise reagents (e.g. enzymes, primers, dNTPs, NTPs, RNAse inhibitors, or buffers) for performing nucleic acid extension reactions, for example, reverse transcription reactions and primer extension reactions. The kit may further comprise reagents (e.g. enzymes, universal primers, sequencing primers, target-specific primers, or buffers) for performing amplification reactions to prepare sequencing libraries.

The kit can, in some embodiments, comprise sequencing library amplification primers of the disclosure. The kit may comprise a second strand synthesis primer of the disclosure. The kit can comprise any primers of the disclosure (e.g., gene-specific primers, random multimers, sequencing primers, and universal primers).

The kit can, in some embodiments, comprise one or more molds, for example, molds comprising an array of micropillars, for casting substrates (e.g., microwell arrays), and one or more solid supports (e.g., bead), wherein the individual beads within a suspension comprise a plurality of attached stochastic barcodes of the disclosure. The kit may further comprise a material for use in casting substrates (e.g. agarose, a hydrogel, PDMS, optical adhesive. and the like).

The kit can, in some embodiments, comprise one or more substrates that are pre-loaded with solid supports comprising a plurality of attached stochastic barcodes of the disclosure. In some instances, there can be one solid support per microwell of the substrate. In some embodiments, the plurality of stochastic barcodes may be attached directly to a surface of the substrate, rather than to a solid support. In any of these embodiments, the one or more microwell arrays can be provided in the form of free-standing substrates (or chips), or they may be packed in flow-cells or cartridges.

In some embodiments, the kit can comprise one or more cartridges that incorporate one or more substrates. In some embodiments, the one or more cartridges further comprises one or more pre-loaded solid supports, wherein the individual solid supports within a suspension comprise a plurality of attached stochastic barcodes of the disclosure. In some embodiments, the beads can be pre-distributed into the one or more microwell arrays of the cartridge. In some embodiments, the beads, in the form of suspensions, can be pre-loaded and stored within reagent wells of the cartridge. In some embodiments, the one or more cartridges may further comprise other assay reagents that are pre-loaded and stored within reagent reservoirs of the cartridges.

Kits can generally include instructions for carrying out one or more of the methods described herein. Instructions included in kits can be affixed to packaging material or can be included as a package insert. While the instructions are typically written or printed materials they are not limited to such. Any medium capable of storing such instructions and communicating them to an end user is contemplated by the disclosure. Such media can include, but are not limited to, electronic storage media (e.g., magnetic discs, tapes, cartridges, chips), optical media (e.g., CD ROM), RF tags, and the like. As used herein, the term “instructions” can include the address of an internet site that provides the instructions.

The kit can comprise the device as described in U.S. application Ser. No. 14/508,911 is herein incorporated by reference in its entirety.

The kit can comprise one or more of the rFit User Guide, SDS for kit reagents, rFit RT Primer Mix, 10 mM Tris HCl, pH 8.0, rFit 2× RT Reaction Mix, rFit RT Enzyme Mix, Spike-in RNA control 1 ng/μL, rFit 2× PCR Master Mix, rFit PCR Primer Mix, Spike-In PCR Primer Mix, Hybridization Buffer Mix, A 16-well detector cartridge with adhesive cover, or any combination thereof.

The kit may require the user to provide certain reagents. For example, the user may need to provide reagents such as: RNase-free water (Ambion, cat no. AM9932), Wash A (Affymetrix, cat no. 900721), Wash B (Affymetrix, cat no. 900722), and Lens Paper (Tiffen, cat no. 154 6027T), or any combination thereof.

The user may need to provide consumables such as RNase-free filter pipette tips (Rainin), 0.2 mL PCR tubes, and 1.5 mL microcentrifuge tubes, or any combination thereof.

The user may need to provide equipment such as: Pipettes (1 μL-1000 μL volume capability), Microcentrifuge for 1.5-2.0 mL tubes, Microcentrifuge for 0.2 mL reaction tubes, Vortexer, Thermal cycler with heated lid, Microplate Incubator/Hybridization Oven (MiuLab, cat no. MT70-2, joyfay.com), and CR Imager (Cellular Research), or any combination thereof.

Sequencing Libraries

Some embodiments disclosed herein provide sequencing libraries for a nucleic acid molecule from a sample comprising a plurality of amplicons, wherein each of the plurality of amplicons comprises from 5′ to 3′: a binding site for a first sequencing primer, a molecular label, a fragment of the nucleic acid molecule and a binding site for a second sequencing primer. In some embodiments, each of the plurality of amplicons comprises the same molecular label. In some embodiments, the fragments of the nucleic acid molecule of the plurality of amplicons cover the entire length of the nucleic acid molecule.

In some embodiments, each of the plurality of amplicons comprises a sample label. In some embodiments, each of the plurality of amplicons comprises the same sample label. In some embodiments, the plurality of amplicons comprises an average size of 250 nt. In some embodiments, the plurality of amplicons comprises an average size of 500 nt. In some embodiments, the nucleic acid molecule has a length of at least 1,500 nt. In some embodiments, the nucleic acid molecule has a length of at least 3,000 nt. In some embodiments, the nucleic acid molecule has a length of at least 5,000 nt. In some embodiments, the sample comprises a single cell. In some embodiments, the sequencing libraries comprise at least 10 amplicons. In some embodiments, the sequencing libraries comprise at least 20 amplicons. In some embodiments, the sequencing libraries comprise at least 50 amplicons. In some embodiments, the sequencing libraries comprise at least 100 amplicons. In some embodiments, the sequencing libraries comprise at least 200 amplicons. In some embodiments, the sequencing libraries comprise at least 500 amplicons. In some embodiments, at least two of the fragments of the nucleic acid molecule overlap with each other. In some embodiments, the at least two of the fragments of the nucleic acid molecule overlap with each other by at least 8 nt. In some embodiments, the at least two of the fragments of the nucleic acid molecule overlap with each other by at least 10 nt. In some embodiments, the at least two of the fragments of the nucleic acid molecule overlap with each other by at least 12 nt. In some embodiments, the at least two of the fragments of the nucleic acid molecule overlap with each other by at least 14 nt.

FIG. 3 shows a schematic illustration of an exemplary sequencing library. An oligonucleotide is used to generate a cDNA 305. An adaptor 310 is ligated to the cDNA 305. Following amplification, fragmentation, circularization and amplification steps as shown in FIG. 2, a sequencing library comprising overlapping fragments of the cDNA 305 and flanked by sequencing primer binding sites 315 and 320 is generated. Non-limiting Exemplary sequencing primers are: 5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′ (SEQ ID NO:3) and 5′-CAAGCAGAAGACGGCATACGAGAT|GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3′ (SEQ ID NO:4).

Methods of Analyzing Sequencing Reads

Some embodiments disclosed herein provide methods for analyzing the sequencing reads of the sequencing libraries disclosed herein. In some embodiments, sequencing reads that contain proper sample labels are sorted into corresponding ‘bins’ to sort sequencings reads from the same sample origin. Within each sample, sequencing reads with the same molecular label are mapped either to the whole transcriptome or a set of expected target sequences. Sequencing reads that map to the same gene or target with the same molecular label are likely from the same original target nucleic acid molecule, such as an mRNA, hence a computational analysis can be performed to 1) count the number of molecular labels found per gene for gene expression profiling; and/or 2) assemble small fragment reads that map to the same gene/transcript and molecular label to determine the full length sequence, partial sequence, and/or splice variants.

Samples

Cells

A sample for use in the method, compositions, systems, and kits of the disclosure can comprise one or more cells. In some embodiments, the cells are cancer cells excised from a cancerous tissue, for example, breast cancer, lung cancer, colon cancer, prostate cancer, ovarian cancer, pancreatic cancer, brain cancer, melanoma and non-melanoma skin cancers, and the like. In some instances, the cells are derived from a cancer but collected from a bodily fluid (e.g. circulating tumor cells). Non-limiting examples of cancers can include, adenoma, adenocarcinoma, squamous cell carcinoma, basal cell carcinoma, small cell carcinoma, large cell undifferentiated carcinoma, chondrosarcoma, and fibrosarcoma.

In some embodiments, the cells are cells that have been infected with virus and contain viral oligonucleotides. In some embodiments, the viral infection can be caused by a virus selected from the group consisting of double-stranded DNA viruses (e.g. adenoviruses, herpes viruses, pox viruses), single-stranded (+ strand or “sense”) DNA viruses (e.g. parvoviruses), double-stranded RNA viruses (e.g. reoviruses), single-stranded (+ strand or sense) RNA viruses (e.g. picornaviruses, togaviruses), single-stranded (− strand or antisense) RNA viruses (e.g. orthomyxoviruses, rhabdoviruses), single-stranded ((+ strand or sense) RNA viruses with a DNA intermediate in their life-cycle) RNA-RT viruses (e.g. retroviruses), and double-stranded DNA-RT viruses (e.g. hepadnaviruses). Exemplary viruses can include, but are not limited to, SARS, HIV, coronaviruses, Ebola, Malaria, Dengue, Hepatitis C, Hepatitis B, and Influenza.

In some embodiments, the cells are bacterial cells. These can include cells from gram-positive bacterial and/or gram-negative bacteria. Examples of bacteria that may be analyzed using the disclosed methods, devices, and systems include, but are not limited to, Actinomedurae, Actinomyces israelii, Bacillus anthracia, Bacillus cereus, Clostridium botulinum, Clostridium difficile, Clostridium perfringens, Clostridium tetani, Corynebacterium, Enterococcus faecalis, Listeria monocytogenes, Nocardia, Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epiderm, Streptococcus mutans, Streptococcus pneumoniae and the like. Gram negative bacteria include, but are not limited to, Afipia felis, Bacteriodes, Bartonella bacilliformis, Bortadella pertussis, Borrelia burgdorferi, Borrelia recurrentis, Brucella, Calymmatobacterium granulomatis, Campylobacter, Escherichia coli, Francisella tularensis, Gardnerella vaginalis, Haemophilius aegyptius, Haemophilius ducreyi, Haemophilius influenziae, Heliobacter pylori, Legionella pneumophila, Leptospira interrogans, Neisseria meningitidia, Porphyromonas gingivalis, Providencia sturti, Pseudomonas aeruginosa, Salmonella enteridis, Salmonella typhi, Serratia marcescens, Shigella boydii, Streptobacillus moniliformis, Streptococcus pyogenes, Treponema pallidum, Vibrio cholerae, Yersinia enterocolitica, Yersinia pestis and the like. Other bacteria may include Myobacterium avium, Myobacterium leprae, Myobacterium tuberculosis, Bartonella henseiae, Chlamydia psittaci, Chlamydia trachomatis, Coxiella burnetii, Mycoplasma pneumoniae, Rickettsia akari, Rickettsia prowazekii, Rickettsia rickettsii, Rickettsia tsutsugamushi, Rickettsia typhi, Ureaplasma urealyticum, Diplococcus pneumoniae, Ehrlichia chafensis, Enterococcus faecium, Meningococci and the like.

In some embodiments, the cells are cells from fungi. Non-limiting examples of fungi that may be analyzed using the disclosed methods, devices, and systems include, but are not limited to, Aspergilli, Candidae, Candida albicans, Coccidioides immitis, Cryptococci, and combinations thereof.

In some embodiments, the cells are cells from protozoans or other parasites. Examples of parasites to be analyzed using the methods, devices, and systems of the present disclosure include, but are not limited to, Balantidium coli, Cryptosporidium parvum, Cyclospora cayatanensis, Encephalitozoa, Entamoeba histolytica, Enterocytozoon bieneusi, Giardia lamblia, Leishmaniae, Plasmodii, Toxoplasma gondii, Trypanosomae, trapezoidal amoeba, worms (e.g., helminthes), particularly parasitic worms including, but not limited to, Nematoda (roundworms, e.g., whipworms, hookworms, pinworms, ascarids, filarids and the like), Cestoda (e.g., tapeworms).

As used herein, the term “cell” can refer to one or more cells. In some embodiments, the cells are normal cells, for example, human cells in different stages of development, or human cells from different organs or tissue types (e.g. white blood cells, red blood cells, platelets, epithelial cells, endothelial cells, neurons, glial cells, fibroblasts, skeletal muscle cells, smooth muscle cells, gametes, or cells from the heart, lungs, brain, liver, kidney, spleen, pancreas, thymus, bladder, stomach, colon, small intestine). In some embodiments, the cells can be undifferentiated human stem cells, or human stem cells that have been induced to differentiate. In some embodiments, the cells can be fetal human cells. The fetal human cells can be obtained from a mother pregnant with the fetus. In some embodiments, the cells are rare cells. A rare cell can be, for example, a circulating tumor cell (CTC), circulating epithelial cell, circulating endothelial cell, circulating endometrial cell, circulating stem cell, stem cell, undifferentiated stem cell, cancer stem cell, bone marrow cell, progenitor cell, foam cell, mesenchymal cell, trophoblast, immune system cell (host or graft), cellular fragment, cellular organelle (e.g. mitochondria or nuclei), pathogen infected cell, and the like.

In some embodiments, the cells are non-human cells, for example, other types of mammalian cells (e.g. mouse, rat, pig, dog, cow, or horse). In some embodiments, the cells are other types of animal or plant cells. In some embodiments, the cells can be any prokaryotic or eukaryotic cells.

In some embodiments, a first cell sample is obtained from a person not having a disease or condition, and a second cell sample is obtained from a person having the disease or condition. In some embodiments, the persons are different. In some embodiments, the persons are the same but cell samples are taken at different time points. In some embodiments, the persons are patients, and the cell samples are patient samples. The disease or condition can be a cancer, a bacterial infection, a viral infection, an inflammatory disease, a neurodegenerative disease, a fungal disease, a parasitic disease, a genetic disorder, or any combination thereof.

In some embodiments, cells suitable for use in the presently disclosed methods can range in size, for example ranging from about 2 micrometers to about 100 micrometers in diameter. In some embodiments, the cells can have diameters of at least 2 micrometers, at least 5 micrometers, at least 10 micrometers, at least 15 micrometers, at least 20 micrometers, at least 30 micrometers, at least 40 micrometers, at least 50 micrometers, at least 60 micrometers, at least 70 micrometers, at least 80 micrometers, at least 90 micrometers, or at least 100 micrometers. In some embodiments, the cells can have diameters of at most 100 micrometers, at most 90 micrometers, at most 80 micrometers, at most 70 micrometers, at most 60 micrometers, at most 50 micrometers, at most 40 micrometers, at most 30 micrometers, at most 20 micrometers, at most 15 micrometers, at most 10 micrometers, at most 5 micrometers, or at most 2 micrometers. The cells can have a diameter of any value within a range, for example from about 5 micrometers to about 85 micrometers. In some embodiments, the cells have diameters of about 10 micrometers.

In some embodiments, the cells are sorted prior to associating one or more of the cells with a bead and/or in a microwell. For example the cells can be sorted by fluorescence-activated cell sorting or magnetic-activated cell sorting, or e.g., by flow cytometry. The cells can be filtered by size. In some instances a retentate contains the cells to be associated with the bead. In some instances the flow through contains the cells to be associated with the bead.

Molecular Barcodes

A molecular barcode can refer to a polynucleotide sequence that may be used to stochastically label (e.g., barcode, tag) a target. A molecular barcode can comprise one or more labels. Exemplary labels include, but are not limited to, a universal label, a cellular label, a molecular label, a sample label, a plate label, a spatial label, and/or a pre-spatial label. A molecular barcode can comprise a 5′amine that may link the molecular barcode to a solid support. The molecular barcode can comprise one or more of a universal label, a cellular label, and a molecular label. The universal label may be 5′-most label. The molecular label may be the 3′-most label. In some instances, the universal label, the cellular label, and the molecular label are in any order. The molecular barcode can comprise a target-binding region. The target-binding region can interact with a target (e.g., target nucleic acid, RNA, mRNA, DNA) in a sample. For example, a target-binding region can comprise an oligo dT sequence which can interact with poly-A tails of mRNAs. In some instances, the labels of the molecular barcode (e.g., universal label, dimension label, spatial label, cellular label, and molecular label) may be separated by 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 or more nucleotides.

A molecular barcode can, in some embodiments, comprise one or more universal labels. The one or more universal labels may be the same for all stochastic barcodes in the set of stochastic barcodes (e.g., attached to a given solid support). In some embodiments, the one or more universal labels may be the same for all molecular barcodes attached to a plurality of beads. In some embodiments, a universal label may comprise a nucleic acid sequence that is capable of hybridizing to a sequencing primer. Sequencing primers may be used for sequencing molecular barcodes comprising a universal label. Sequencing primers (e.g., universal sequencing primers) may comprise sequencing primers associated with high-throughput sequencing platforms. In some embodiments, a universal label may comprise a nucleic acid sequence that is capable of hybridizing to a PCR primer. In some embodiments, the universal label may comprise a nucleic acid sequence that is capable of hybridizing to a sequencing primer and a PCR primer. The nucleic acid sequence of the universal label that is capable of hybridizing to a sequencing or PCR primer may be referred to as a primer binding site. A universal label may comprise a sequence that may be used to initiate transcription of the stochastic barcode. A universal label may comprise a sequence that may be used for extension of the stochastic barcode or a region within the stochastic barcode. A universal label may be at least about 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. A universal label may comprise at least about 10 nucleotides. A universal label may be at most about 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. In some embodiments, a cleavable linker or modified nucleotide may be part of the universal label sequence to enable the molecular barcode to be cleaved off from the support. As used herein, a universal label can be used interchangeably with “universal PCR primer.”

A molecular barcode can comprise a dimension label. A dimension label can comprise a nucleic acid sequence that provides information about a dimension in which the stochastic labeling occurred. For example, a dimension label can provide information about the time at which a target was stochastically barcoded. A dimension label can be associated with a time of stochastic barcoding in a sample. A dimension label can activated at the time of molecular labeling. Different dimension labels can be activated at different times. The dimension label provides information about the order in which targets, groups of targets, and/or samples were stochastically barcoded. For example, a population of cells can be stochastically barcoded at the G0 phase of the cell cycle. The cells can be pulsed again with stochastic barcodes at the G1 phase of the cell cycle. The cells can be pulsed again with stochastic barcodes at the S phase of the cell cycle, and so on. Stochastic barcodes at each pulse (e.g., each phase of the cell cycle), can comprise different dimension labels. In this way, the dimension label provides information about which targets were labelled at which phase of the cell cycle. Dimension labels can interrogate many different biological times. Exemplary biological times can include, but are not limited to, the cell cycle, transcription (e.g., transcription initiation), and transcript degradation. In another example, a sample (e.g., a cell, a population of cells) can be stochastically labeled before and/or after treatment with a drug and/or therapy. The changes in the number of copies of distinct targets can be indicative of the sample's response to the drug and/or therapy.

A dimension label can be activatable. An activatable dimension label can be activated at a specific timepoint. The activatable dimension label may be constitutively activated (e.g., not turned off). The activatable dimension label can be reversibly activated (e.g., the activatable dimension label can be turned on and turned off). The dimension label can be reversibly activatable at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 or more times. The dimension label can be reversibly activatable at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 or more times. The dimension label can be activated with fluorescence, light, a chemical event (e.g., cleavage, ligation of another molecule, addition of modifications (e.g., pegylated, sumoylated, acetylated, methylated, deacetylated, demethylated), a photochemical event (e.g., photocaging, photocleavage), and introduction of a non-natural nucleotide.

The dimension label can be identical for all molecular barcodes attached to a given solid support (e.g., bead), but different for different solid supports (e.g., beads). In some embodiments, at least 60%, 70%, 80%, 85%, 90%, 95%, 97%, 99% or 100% of molecular barcodes on the same solid support may comprise the same dimension label. In some embodiments, at least 60% of molecular barcodes on the same solid support may comprise the same dimension label. In some embodiments, at least 95% of molecular barcodes on the same solid support may comprise the same dimension label.

There may be as many as 106 or more unique dimension label sequences represented in a plurality of solid supports (e.g., beads). A dimension label may be at least about 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. A dimension label may be at most about 300, 200, 100, 90, 80, 70, 60, 50, 40, 30, 20, 15, 12, 10, 9, 8, 7, 6, 5, 4 or fewer or more nucleotides in length. A dimension label may comprise from about 5 to about 200 nucleotides. A dimension label may comprise from about 10 to about 150 nucleotides. A dimension label may comprise from about 20 to about 125 nucleotides in length.

A molecular barcode can comprise a spatial label. A spatial label can comprise a nucleic acid sequence that provides information about the spatial orientation of a target molecule which is associated with the stochastic barcode. A spatial label can be associated with a coordinate in a sample. The coordinate can be a fixed coordinate. For example a coordinate can be fixed in reference to a substrate. A spatial label can be in reference to a two or three-dimensional grid. A coordinate can be fixed in reference to a landmark. The landmark can be identifiable in space. A landmark can a structure which can be imaged. A landmark can be a biological structure, for example an anatomical landmark. A landmark can be a cellular landmark, for instance an organelle. A landmark can be a non-natural landmark such as a structure with an identifiable identifier such as a color code, bar code, magnetic property, fluorescents, radioactivity, or a unique size or shape. A spatial label can be associated with a physical partition (e.g. a well, a container, or a droplet). In some instances, multiple spatial labels are used together to encode one or more positions in space.

The spatial label can be identical for all stochastic barcodes attached to a given solid support (e.g., bead), but different for different solid supports (e.g., beads). In some embodiments, at least 60%, 70%, 80%, 85%, 90%, 95%, 97%, 99% or 100% of molecular barcodes on the same solid support may comprise the same spatial label. In some embodiments, at least 60% of stochastic barcodes on the same solid support may comprise the same spatial label. In some embodiments, at least 95% of molecular barcodes on the same solid support may comprise the same spatial label.

There may be as many as 106 or more unique spatial label sequences represented in a plurality of solid supports (e.g., beads). A spatial label may be at least about 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. A spatial label may be at most about 300, 200, 100, 90, 80, 70, 60, 50, 40, 30, 20, 15, 12, 10, 9, 8, 7, 6, 5, 4 or fewer or more nucleotides in length. A spatial label may comprise from about 5 to about 200 nucleotides. A spatial label may comprise from about 10 to about 150 nucleotides. A spatial label may comprise from about 20 to about 125 nucleotides in length.

Molecular barcodes may comprise a cellular label. A cellular label may comprise a nucleic acid sequence that provides information for determining which target nucleic acid originated from which cell. In some embodiments, the cellular label is identical for all stochastic barcodes attached to a given solid support (e.g., bead), but different for different solid supports (e.g., beads). In some embodiments, at least 60%, 70%, 80%, 85%, 90%, 95%, 97%, 99% or 100% of stochastic barcodes on the same solid support may comprise the same cellular label. In some embodiments, at least 60% of stochastic barcodes on the same solid support may comprise the same cellular label. In some embodiment, at least 95% of molecular barcodes on the same solid support may comprise the same cellular label.

There may be as many as 106 or more unique cellular label sequences represented in a plurality of solid supports (e.g., beads). A cellular label may be at least about 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. A cellular label may be at most about 300, 200, 100, 90, 80, 70, 60, 50, 40, 30, 20, 15, 12, 10, 9, 8, 7, 6, 5, 4 or fewer or more nucleotides in length. A cellular label may comprise from about 5 to about 200 nucleotides. A cellular label may comprise from about 10 to about 150 nucleotides. A cellular label may comprise from about 20 to about 125 nucleotides in length.

Molecular barcodes may comprise a molecular label. A molecular label may comprise a nucleic acid sequence that provides identifying information for the specific type of target nucleic acid species hybridized to the stochastic barcode. A molecular label may comprise a nucleic acid sequence that provides a counter for the specific occurrence of the target nucleic acid species hybridized to the stochastic barcode (e.g., target-binding region). In some embodiments, a diverse set of molecular labels are attached to a given solid support (e.g., bead). In some embodiments, there may be as many as 106 or more unique molecular label sequences attached to a given solid support (e.g., bead). In some embodiments, there may be as many as 105 or more unique molecular label sequences attached to a given solid support (e.g., bead). In some embodiments, there may be as many as 104 or more unique molecular label sequences attached to a given solid support (e.g., bead). In some embodiments, there may be as many as 103 or more unique molecular label sequences attached to a given solid support (e.g., bead). In some embodiments, there may be as many as 102 or more unique molecular label sequences attached to a given solid support (e.g., bead). A molecular label may be at least about 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. A molecular label may be at most about 300, 200, 100, 90, 80, 70, 60, 50, 40, 30, 20, 15, 12, 10, 9, 8, 7, 6, 5, 4 or fewer nucleotides in length.

Molecular barcodes may comprise a target binding region. In some embodiments, the target binding regions may comprise a nucleic acid sequence that hybridizes specifically to a target (e.g., target nucleic acid, target molecule, e.g., a cellular nucleic acid to be analyzed), for example to a specific gene sequence. In some embodiments, a target binding region may comprise a nucleic acid sequence that may attach (e.g., hybridize) to a specific location of a specific target nucleic acid. In some embodiments, the target binding region may comprise a nucleic acid sequence that is capable of specific hybridization to a restriction site overhang (e.g. an EcoRI sticky-end overhang). The molecular barcode may then ligate to any nucleic acid molecule comprising a sequence complementary to the restriction site overhang.

A molecular barcode can comprise a target-binding region. A target-binding region can hybridize with a target of interest. For example, a target-binding region can comprise an oligo dT which can hybridize with mRNAs comprising poly-adenylated ends. A target-binding region can be gene-specific. For example, a target-binding region can be configured to hybridize to a specific region of a target. A target-binding region can be at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 27, 28, 29, or 30 or more nucleotides in length. A target-binding region can be at most 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 27, 28, 29, or 30 or more nucleotides in length. A target-binding region can be from 5-30 nucleotides in length. When a stochastic barcode comprises a gene-specific target-binding region, the stochastic barcode can be referred to as a gene-specific stochastic barcode.

A target binding region may comprise a non-specific target nucleic acid sequence. A non-specific target nucleic acid sequence may refer to a sequence that may bind to multiple target nucleic acids, independent of the specific sequence of the target nucleic acid. For example, target binding region may comprise a random multimer sequence, or an oligo-dT sequence that hybridizes to the poly-A tail on mRNA molecules. A random multimer sequence can be, for example, a random dimer, trimer, quatramer, pentamer, hexamer, septamer, octamer, nonamer, decamer, or higher multimer sequence of any length. In some embodiments, the target binding region is the same for all stochastic barcodes attached to a given bead. In some embodiments, the target binding regions for the plurality of stochastic barcodes attached to a given bead may comprise two or more different target binding sequences. A target binding region may be at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. A target binding region may be at most about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length.

A molecular barcode can comprise an orientation property which can be used to orient (e.g., align) the stochastic barcodes. A molecular barcode can comprise a moiety for isoelectric focusing. Different molecular barcodes can comprise different isoelectric focusing points. When these molecular barcodes are introduced to a sample, the sample can undergo isoelectric focusing in order to orient the stochastic barcodes into a known way. In this way, the orientation property can be used to develop a known map of stochastic barcodes in a sample. Exemplary orientation properties can include, electrophoretic mobility (e.g., based on size of the stochastic barcode), isoelectric point, spin, conductivity, and/or self-assembly. For example, molecular barcodes can comprise an orientation property of self-assembly, can self-assemble into a specific orientation (e.g., nucleic acid nanostructure) upon activation.

A molecular barcode can comprise an affinity property. A spatial label can comprise an affinity property. An affinity property can be include a chemical and/or biological moiety that can facilitate binding of the stochastic barcode to another entity (e.g., cell receptor).

The cellular label and/or any label of the disclosure may further comprise a unique set of nucleic acid sub-sequences of defined length, e.g. 7 nucleotides each (equivalent to the number of bits used in some Hamming error correction codes), which are designed to provide error correction capability. The set of error correction sub-sequences comprise 7 nucleotide sequences can be designed such that any pairwise combination of sequences in the set exhibits a defined “genetic distance” (or number of mismatched bases), for example, a set of error correction sub-sequences may be designed to exhibit a genetic distance of 3 nucleotides. In some embodiments, the length of the nucleic acid sub-sequences used for creating error correction codes may vary, for example, they may be at least 3 nucleotides, at least 7 nucleotides, at least 15 nucleotides, or at least 31 nucleotides in length. In some embodiments, nucleic acid sub-sequences of other lengths may be used for creating error correction codes.

Molecular barcodes of the disclosure can comprise error-correcting sequences (e.g., Hamming codes) in them for error-correction. A Hamming code can refer an arithmetic process that identifies unique binary codes based upon inherent redundancy that are capable of correcting single bit errors. For example, a Hamming code can be matched with a nucleic acid barcode in order to screen for single nucleotide errors occurring during nucleic acid amplification. The identification of a single nucleotide error by using a Hamming code, thereby can allow for the correction of the nucleic acid barcode.

When a molecular barcode comprises more than one of a type of label (e.g., more than one cellular label or more than one molecular label), the labels may be interspersed with a linker label sequence. A linker label sequence may be at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. A linker label sequence may be at most about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more nucleotides in length. In some instances, a linker label sequence is 12 nucleotides in length. A linker label sequence may be used to facilitate the synthesis of the molecular barcode. The linker label can comprise an error-correcting (e.g., Hamming) code.

Solid Supports and Substrates

The stochastic barcodes disclosed herein can be attached to a solid support (e.g., bead, substrate). As used herein, the terms “tethered”, “attached”, and “immobilized” are used interchangeably, and may refer to covalent or non-covalent means for attaching stochastic barcodes to a solid support. Any of a variety of different solid supports may be used as solid supports for attaching pre-synthesized stochastic barcodes or for in situ solid-phase synthesis of stochastic barcode.

In some instances, a solid support is a bead. A bead may encompass any type of solid, porous, or hollow sphere, ball, bearing, cylinder, or other similar configuration composed of plastic, ceramic, metal, or polymeric material onto which a nucleic acid may be immobilized (e.g., covalently or non-covalently). A bead can, in some embodiments, comprise a discrete particle that may be spherical (e.g., microspheres) or have a non-spherical or irregular shape, such as cubic, cuboid, pyramidal, cylindrical, conical, oblong, or disc-shaped, and the like. A bead may be non-spherical in shape.

Beads can comprise a variety of materials including, but not limited to, paramagnetic materials (e.g. magnesium, molybdenum, lithium, and tantalum), superparamagnetic materials (e.g. ferrite (Fe3O4; magnetite) nanoparticles), ferromagnetic materials (e.g. iron, nickel, cobalt, some alloys thereof, and some rare earth metal compounds), ceramic, plastic, glass, polystyrene, silica, methylstyrene, acrylic polymers, titanium, latex, sepharose, agarose, hydrogel, polymer, cellulose, nylon, and any combination thereof.

The diameter of the beads can, in some embodiments, be at least about 5 μm, 10 μm, 20 μm, 25 μm, 30 μm, 35 μm, 40 μm, 45 μm or 50 μm. The diameter of the beads can, in some embodiments, be at most about 5 μm, 10 μm, 20 μm, 25 μm, 30 μm, 35 μm, 40 μm, 45 μm or 50 μm. The diameter of the bead may be related to the diameter of the wells of the substrate. For example, the diameter of the bead may be at least 10, 20, 30, 40, 50, 60, 70, 80, 90 or 100% longer or shorter than the diameter of the well. The diameter of the bead can, in some embodiments, be at most 10, 20, 30, 40, 50, 60, 70, 80, 90 or 100% longer or shorter than the diameter of the well. The diameter of the bead may be related to the diameter of a cell (e.g., a single cell entrapped by the a well of the substrate). The diameter of the bead may be at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200, 250, or 300% or more longer or shorter than the diameter of the cell. The diameter of the bead may be at most 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200, 250, or 300% or more longer or shorter than the diameter of the cell.

A bead can, in some embodiments, be attached to and/or embedded in a substrate of the disclosure. A bead may be attached to and/or embedded in a gel, hydrogel, polymer and/or matrix. The spatial position of a bead within a substrate (e.g., gel, matrix, scaffold, or polymer) may be identified using the spatial label present on the stochastic barcode on the bead which can serve as a location address.

Examples of beads can include, but are not limited to, streptavidin beads, agarose beads, magnetic beads, Dynabeads®, MACS® microbeads, antibody conjugated beads (e.g., anti-immunoglobulin microbead), protein A conjugated beads, protein G conjugated beads, protein A/G conjugated beads, protein L conjugated beads, oligodT conjugated beads, silica beads, silica-like beads, anti-biotin microbead, anti-fluorochrome microbead, and BcMag™ Carboxy-Terminated Magnetic Beads.

A bead can, in some embodiments, be associated with (e.g. impregnated with) quantum dots or fluorescent dyes to make it fluorescent in one fluorescence optical channel or multiple optical channels. A bead may be associated with iron oxide or chromium oxide to make it paramagnetic or ferromagnetic. Beads can be identifiable. A bead can be imaged using a camera. A bead can have a detectable code associated with the bead. For example, a bead can comprise an RFID tag. A bead can comprise any detectable tag (e.g., UPC code, electronic barcode, etched identifier). A bead can change size, for example due to swelling in an organic or inorganic solution. A bead can be hydrophobic. A bead can be hydrophilic. A bead can be biocompatible.

A solid support (e.g., bead) can be visualized. The solid support can comprise a visualizing tag (e.g., fluorescent dye). A solid support (e.g., bead) can be etched with an identifier (e.g., a number). The identifier can be visualized through imaging the solid supports (e.g., beads).

A solid support may refer to an insoluble, semi-soluble, or insoluble material. A solid support may be referred to as “functionalized” when it includes a linker, a scaffold, a building block, or other reactive moiety attached thereto, whereas a solid support may be “nonfunctionalized” when it lack such a reactive moiety attached thereto. The solid support may be employed free in solution, such as in a microtiter well format; in a flow-through format, such as in a column; or in a dipstick.

The solid support can, in some embodiments, comprise a membrane, paper, plastic, coated surface, flat surface, glass, slide, chip, or any combination thereof. A solid support may take the form of resins, gels, microspheres, or other geometric configurations. A solid support can comprise silica chips, microparticles, nanoparticles, plates, arrays, capillaries, flat supports such as glass fiber filters, glass surfaces, metal surfaces (steel, gold silver, aluminum, silicon and copper), glass supports, plastic supports, silicon supports, chips, filters, membranes, microwell plates, slides, plastic materials including multiwell plates or membranes (e.g., formed of polyethylene, polypropylene, polyamide, polyvinylidenedifluoride), and/or wafers, combs, pins or needles (e.g., arrays of pins suitable for combinatorial synthesis or analysis) or beads in an array of pits or nanoliter wells of flat surfaces such as wafers (e.g., silicon wafers), wafers with pits with or without filter bottoms.

The solid support can comprise a polymer matrix (e.g., gel, hydrogel). The polymer matrix may be able to permeate intracellular space (e.g., around organelles). The polymer matrix may able to be pumped throughout the circulatory system.

A solid support can be a biological molecule. For example a solid support can be a nucleic acid, a protein, an antibody, a histone, a cellular compartment, a lipid, a carbohydrate, and the like. Solid supports that are biological molecules can be amplified, translated, transcribed, degraded, and/or modified (e.g., pegylated, sumoylated, acetylated, methylated). A solid support that is a biological molecule can provide spatial and time information in addition to the spatial label that is attached to the biological molecule. For example, a biological molecule can comprise a first confirmation when unmodified, but can change to a second confirmation when modified. The different conformations can expose stochastic barcodes of the disclosure to targets. For example, a biological molecule can comprise stochastic barcodes that are unaccessible due to folding of the biological molecule. Upon modification of the biological molecule (e.g., acetylation), the biological molecule can change conformation to expose the stochastic labels. The timing of the modification can provide another time dimension to the method of stochastic barcoding of the disclosure.

In another example, the biological molecule comprising stochastic barcodes of the disclosure can be located in the cytoplasm of a cell. Upon activation, the biological molecule can move to the nucleus, whereupon stochastic barcoding can take place. In this way, modification of the biological molecule can encode additional space-time information for the targets identified by the stochastic barcodes.

A dimension label can provide information about space-time of a biological event (e.g., cell division). For example, a dimension label can be added to a first cell, the first cell can divide generating a second daughter cell, the second daughter cell can comprise all, some or none of the dimension labels. The dimension labels can be activated in the original cell and the daughter cell. In this way, the dimension label can provide information about time of stochastic barcoded in distinct spaces.

Some aspects of the embodiments discussed above are disclosed in further detail in the following examples, which are not in any way intended to limit the scope of the present disclosure.

1 ng RNA of T cell with Kan/Dap/Phe spike-in RNA was reverse transcribed using an oligonucleotide that was phosphorylated at 5′ and circularization friendly comprising, from 5′ to 3′: a binding site for a universal primer, a sample label, a molecular label, a binding site for a P5 primer in the opposite orientation, and oligo-dT. The cDNA produced were purified using AMPure® and PCR amplified for 30 cycles using the universal primer and gene-specific primers (TCR, KDP F, Anchor R (ES29)). The PCR products were cleaned up with AMPure® and circularized using CircLigase™ I at 60° C. for 1 hr followed by 80° C. for 10 min. The circularized DNA was treated with ExoI at 37° C., 30 min, and at 80° C., 20 min. 1 μL of the treated product was PCR amplified using N1R and R primers and OneTaq® DNA polymerase. 1 μL of the PCR product was PCR amplified using P5 and P7 primers and OneTaq® DNA polymerase. The sequencing library from the last PCR step was sequenced using Illumina MiSeq v3 for 2×75 bp reads. FIG. 4 shows the results from sequencing and data analysis of the raw sequencing reads. Modified F primer mapping algorithm was used to exclude duplicated reads that map to 1+ primers.

While various aspects and embodiments have been disclosed herein, other aspects and embodiments will be apparent to those skilled in the art. The various aspects and embodiments disclosed herein are for purposes of illustration and are not intended to be limiting, with the true scope and spirit being indicated by the following claims.

One skilled in the art will appreciate that, for this and other processes and methods disclosed herein, the functions performed in the processes and methods can be implemented in differing order. Furthermore, the outlined steps and operations are only provided as examples, and some of the steps and operations can be optional, combined into fewer steps and operations, or expanded into additional steps and operations without detracting from the essence of the disclosed embodiments.

With respect to the use of substantially any plural and/or singular terms herein, those having skill in the art can translate from the plural to the singular and/or from the singular to the plural as is appropriate to the context and/or application. The various singular/plural permutations may be expressly set forth herein for sake of clarity.

It will be understood by those within the art that, in general, terms used herein, and especially in the appended claims (e.g., bodies of the appended claims) are generally intended as “open” terms (e.g., the term “including” should be interpreted as “including but not limited to,” the term “having” should be interpreted as “having at least,” the term “includes” should be interpreted as “includes but is not limited to,” etc.). It will be further understood by those within the art that if a specific number of an introduced claim recitation is intended, such an intent will be explicitly recited in the claim, and in the absence of such recitation no such intent is present. For example, as an aid to understanding, the following appended claims may contain usage of the introductory phrases “at least one” and “one or more” to introduce claim recitations. However, the use of such phrases should not be construed to imply that the introduction of a claim recitation by the indefinite articles “a” or “an” limits any particular claim containing such introduced claim recitation to embodiments containing only one such recitation, even when the same claim includes the introductory phrases “one or more” or “at least one” and indefinite articles such as “a” or “an” (e.g., “a” and/or “an” should be interpreted to mean “at least one” or “one or more”); the same holds true for the use of definite articles used to introduce claim recitations. In addition, even if a specific number of an introduced claim recitation is explicitly recited, those skilled in the art will recognize that such recitation should be interpreted to mean at least the recited number (e.g., the bare recitation of “two recitations,” without other modifiers, means at least two recitations, or two or more recitations). Furthermore, in those instances where a convention analogous to “at least one of A, B, and C, etc.” is used, in general such a construction is intended in the sense one having skill in the art would understand the convention (e.g., “a system having at least one of A, B, and C” would include but not be limited to systems that have A alone, B alone, C alone, A and B together, A and C together, B and C together, and/or A, B, and C together, etc.). In those instances where a convention analogous to “at least one of A, B, or C, etc.” is used, in general such a construction is intended in the sense one having skill in the art would understand the convention (e.g., “a system having at least one of A, B, or C” would include but not be limited to systems that have A alone, B alone, C alone, A and B together, A and C together, B and C together, and/or A, B, and C together, etc.). It will be further understood by those within the art that virtually any disjunctive word and/or phrase presenting two or more alternative terms, whether in the description, claims, or drawings, should be understood to contemplate the possibilities of including one of the terms, either of the terms, or both terms. For example, the phrase “A or B” will be understood to include the possibilities of “A” or “B” or “A and B.”

In addition, where features or aspects of the disclosure are described in terms of Markush groups, those skilled in the art will recognize that the disclosure is also thereby described in terms of any individual member or subgroup of members of the Markush group.

As will be understood by one skilled in the art, for any and all purposes, such as in terms of providing a written description, all ranges disclosed herein also encompass any and all possible subranges and combinations of subranges thereof. Any listed range can be easily recognized as sufficiently describing and enabling the same range being broken down into at least equal halves, thirds, quarters, fifths, tenths, etc. As a non-limiting example, each range discussed herein can be readily broken down into a lower third, middle third and upper third, etc. As will also be understood by one skilled in the art all language such as “up to,” “at least,” and the like include the number recited and refer to ranges which can be subsequently broken down into subranges as discussed above. Finally, as will be understood by one skilled in the art, a range includes each individual member. Thus, for example, a group having 1-3 cells refers to groups having 1, 2, or 3 cells. Similarly, a group having 1-5 cells refers to groups having 1, 2, 3, 4, or 5 cells, and so forth.

From the foregoing, it will be appreciated that various embodiments of the present disclosure have been described herein for purposes of illustration, and that various modifications may be made without departing from the scope and spirit of the present disclosure. Accordingly, the various embodiments disclosed herein are not intended to be limiting, with the true scope and spirit being indicated by the following claims.

Shum, Eleen, Fu, Glenn

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/////
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