A sample fluid (14) is pumped through a first cavity (38) associated with a first piezoelectric resonator (20.1) and pumped through a second cavity (40) associated with a second piezoelectric resonator (20.2). An electrode (26) of the first piezoelectric resonator (20.1) exposed to the sample fluid (14) in the first cavity (38) is coated with an adsorption layer (34.1) that provides for adsorbing a substance (12) to be detected in the sample fluid (14). The adsorbed substance (12) changes the resonant frequency of the first piezoelectric resonator (20.1) relative to that of the second piezoelectric resonator (20.2), wherein a change in the frequency difference therebetween relative to an initial frequency difference is responsive to and provides a measure of the mass of adsorbed substance (12). The adsorption layer (34.1) of the first piezoelectric resonator (20.1) is automatically refreshed when a change in the frequency difference crosses a threshold (ΔFEOR).
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1. A method of sensing a substance in a sample fluid, comprising:
a. automatically resonating a first piezoelectric resonator associated with a first cavity, wherein said first piezoelectric resonator comprises:
i. a first portion of piezoelectric material, wherein said first portion of piezoelectric material has opposing first and second surfaces spanning a thickness of said first portion of piezoelectric material, and a lateral extent of said first portion of piezoelectric material underlying said first and second surfaces is substantially greater than said thickness of said first portion of piezoelectric material;
ii. a first electrode on said first surface of said first portion of piezoelectric material, wherein a first side of said first electrode is attached to said first surface of said first portion of piezoelectric material, a second side of said first electrode is exposed to a first fluid in said first cavity, and said second side of said first electrode incorporates a coating that provides for preferentially adsorbing the substance if said substance is contained in said first fluid; and
iii. a second electrode on said second surface of said first portion of piezoelectric material, wherein a first side of said second electrode is attached to said second surface of said first portion of piezoelectric material, and the operation of automatically resonating said first piezoelectric resonator comprises applying a first time-varying voltage across said first and second electrodes at a first frequency sufficient to cause said first portion of piezoelectric material to mechanically resonate;
b. automatically resonating a second piezoelectric resonator associated with a second cavity, wherein said second cavity is distinct from said first cavity, and said second piezoelectric resonator comprises:
i. a second portion of piezoelectric material, wherein said piezoelectric material of said second portion of piezoelectric material is either distinct from, or continuous with, said piezoelectric material of said first portion of piezoelectric material, said second portion of piezoelectric material has opposing third and fourth surfaces spanning a thickness of said second portion of piezoelectric material, and a lateral extent of said second portion of piezoelectric material underlying said third and fourth surfaces is substantially greater than said thickness of said second portion of piezoelectric material;
ii. a third electrode on said third surface of said second portion of piezoelectric material, wherein a first side of said third electrode is attached to said third surface of said second portion of piezoelectric material, and a second side of said third electrode is exposed to a second fluid in said second cavity; and
iii. a fourth electrode on said fourth surface of said second portion of piezoelectric material, wherein a first side of said fourth electrode is attached to said fourth surface of said second portion of piezoelectric material, and the operation of automatically resonating said second piezoelectric resonator comprises applying a second time-varying voltage across said third and fourth electrodes at a second frequency sufficient to cause said second portion of piezoelectric material to mechanically resonate, wherein a frequency difference by which said second frequency differs from said first frequency is responsive to an amount of said substance adsorbed by said coating on said second side of said first electrode of said first portion of piezoelectric material of said first piezoelectric resonator;
c. automatically commencing or continuing a sampling process, wherein said sampling process comprises:
i. automatically pumping the sample fluid as said first fluid through said first cavity and detecting said first frequency responsive thereto; and
ii. automatically pumping said sample fluid as said second fluid through said second cavity and detecting said second frequency responsive thereto;
d. continuing with said sampling process until a change in said frequency difference, by which said second frequency differs from said first frequency during said sampling process, relative to an initial frequency difference, crosses a first threshold, then automatically interrupting said sampling process and automatically commencing a refresh process to provide for refreshing said coating incorporated on said second side of said first electrode, wherein said refresh process comprises:
i. automatically pumping a regeneration fluid as said first fluid through said first cavity, and detecting said first frequency responsive thereto;
ii. automatically pumping a neutral fluid as said second fluid through said second cavity, and detecting said second frequency responsive thereto;
e. continuing said refresh process until said change in said frequency difference, by which said second frequency differs from said first frequency during said refresh process, crosses a second threshold; and
f. automatically terminating said refresh process, and automatically resuming said sampling process at step c.
47. A method of providing for sensing a substance in a sample fluid, comprising:
a. providing for automatically resonating a first piezoelectric resonator associated with a first cavity, wherein said first piezoelectric resonator comprises:
i. a first portion of piezoelectric material, wherein said first portion of piezoelectric material has opposing first and second surfaces spanning a thickness of said first portion of piezoelectric material, and a lateral extent of said first portion of piezoelectric material underlying said first and second surfaces is substantially greater than said thickness of said first portion of piezoelectric material;
ii. a first electrode on said first surface of said first portion of piezoelectric material, wherein a first side of said first electrode is attached to said first surface of said first portion of piezoelectric material, a second side of said first electrode is exposed to a first fluid in said first cavity, and said second side of said first electrode incorporates a coating that provides for preferentially adsorbing the substance if said substance is contained in said first fluid; and
iii. a second electrode on said second surface of said first portion of piezoelectric material, wherein a first side of said second electrode is attached to said second surface of said first portion of piezoelectric material, and the operation of providing for automatically resonating said first piezoelectric resonator comprises providing for applying a first time-varying voltage across said first and second electrodes at a first frequency sufficient to cause said first portion of piezoelectric material to mechanically resonate;
b. providing for automatically resonating a second piezoelectric resonator associated with a second cavity, wherein said second cavity is distinct from said first cavity, and said second piezoelectric resonator comprises:
i. a second portion of piezoelectric material, wherein said piezoelectric material of said second portion of piezoelectric material is either distinct from, or continuous with, said piezoelectric material of said first portion of piezoelectric material, said second portion of piezoelectric material has opposing third and fourth surfaces spanning a thickness of said second portion of piezoelectric material, and a lateral extent of said second portion of piezoelectric material underlying said third and fourth surfaces is substantially greater than said thickness of said second portion of piezoelectric material;
ii. a third electrode on said third surface of said second portion of piezoelectric material, wherein a first side of said third electrode is attached to said third surface of said second portion of piezoelectric material, and a second side of said third electrode is exposed to a second fluid in said second cavity; and
iii. a fourth electrode on said fourth surface of said second portion of piezoelectric material, wherein a first side of said fourth electrode is attached to said fourth surface of said second portion of piezoelectric material, and the operation of providing for automatically resonating said second piezoelectric resonator comprises providing for applying a second time-varying voltage across said third and fourth electrodes at a second frequency sufficient to cause said second portion of piezoelectric material to mechanically resonate, wherein a frequency difference by which said second frequency differs from said first frequency is responsive to an amount of said substance adsorbed by said coating on said second side of said first electrode of said first portion of piezoelectric material of said first piezoelectric resonator;
c. providing for automatically commencing or continuing a sampling process, wherein said sampling process comprises:
i. automatically pumping the sample fluid as said first fluid through said first cavity and detecting said first frequency responsive thereto; and
ii. automatically pumping said sample fluid as said second fluid through said second cavity and detecting said second frequency responsive thereto;
d. providing for continuing with said sampling process until a change in said frequency difference, by which said second frequency differs from said first frequency during said sampling process, relative to an initial frequency difference, crosses a first threshold, then providing for automatically interrupting said sampling process and providing for automatically commencing a refresh process to provide for refreshing said coating incorporated on said second side of said first electrode, wherein said refresh process comprises:
i. automatically pumping a regeneration fluid as said first fluid through said first cavity, and detecting said first frequency responsive thereto;
ii. automatically pumping a neutral fluid as said second fluid through said second cavity, and detecting said second frequency responsive thereto;
e. providing for continuing said refresh process until said change in said frequency difference, by which said second frequency differs from said first frequency during said refresh process, crosses a second threshold; and
f. providing for automatically terminating said refresh process, and providing for automatically resuming said sampling process at step c.
24. A system for sensing a substance in a sample fluid, comprising:
a. a first sensor, wherein said first sensor comprises a first piezoelectric resonator, and said first piezoelectric resonator comprises:
i. a first portion of piezoelectric material, wherein said first portion of piezoelectric material has opposing first and second surfaces spanning a thickness of said first portion of piezoelectric material, and a lateral extent of said first portion of piezoelectric material underlying said first and second surfaces is substantially greater than said thickness of said first portion of piezoelectric material;
ii. a first electrode on said first surface of said first portion of piezoelectric material, wherein a first side of said first electrode is attached to said first surface of said first portion of piezoelectric material;
iii. a second electrode on said second surface of said first portion of piezoelectric material, wherein a first side of said second electrode is attached to said second surface of said first portion of piezoelectric material; and
iv. a first cavity, wherein said first cavity cooperates with a first inlet and a first outlet, said first cavity is closed except at said first inlet and said first outlet, a second side of said first electrode is exposed to a first fluid in said first cavity, and said second side of said first electrode incorporates a coating that provides for preferentially adsorbing the substance to be detected if said substance is contained in said first fluid, and said first cavity is bounded in part by said first surface of said first portion of piezoelectric material or by the coated first electrode thereon;
b. a second sensor, wherein said second sensor comprises a second piezoelectric resonator, and said second piezoelectric resonator comprises:
i. a second portion of piezoelectric material, wherein said piezoelectric material of said second portion of piezoelectric material is either distinct from, or continuous with, said piezoelectric material of said first portion of piezoelectric material, said second portion of piezoelectric material has opposing third and fourth surfaces spanning a thickness of said second portion of piezoelectric material, and a lateral extent of said second portion of piezoelectric material underlying said third and fourth surfaces is substantially greater than said thickness of said second portion of piezoelectric material;
ii. a third electrode on said third surface of said second portion of piezoelectric material, wherein a first side of said third electrode is attached to said third surface of said second portion of piezoelectric material;
iii. a fourth electrode on said fourth surface of said second portion of piezoelectric material, wherein a first side of said fourth electrode is attached to said fourth surface of said second portion of piezoelectric material; and
iv. a second cavity, wherein said second cavity cooperates with a second inlet and a second outlet, said second cavity is closed except at said second inlet and said second outlet, a second side of said third electrode is exposed to a second fluid in said second cavity, and said second cavity is bounded in part by said third surface of said second portion of piezoelectric material or by said third electrode thereon;
c. at least one piezoelectric resonator drive circuit, wherein each piezoelectric resonator drive circuit of said at least one piezoelectric resonator drive circuit comprises:
i. an oscillator driver to which at least one of said first and second piezoelectric resonators is connected, wherein at any given time, one of said first and second piezoelectric resonators provides for controlling a frequency of oscillation of said oscillator driver so as to oscillate at a resonant frequency of said one of said first and second piezoelectric resonators; and
ii. a frequency detector for determining a magnitude of said frequency of oscillation;
d. a controller operatively coupled to said at least one piezoelectric resonator drive circuit, wherein said controller provides for determining at least one measure of, or responsive to, a mass of said substance adsorbed on said second side of said first electrode, responsive to a change in frequency difference relative to an initial frequency difference, wherein said change in said frequency difference is responsive to an amount by which a second frequency corresponding to said frequency of oscillation of said second piezoelectric resonator differs from a first frequency corresponding to said frequency of oscillation of said first piezoelectric resonator;
e. a source of neutral fluid, wherein said neutral fluid, when in said first cavity, has substantially no effect on said substance if said substance has been adsorbed by said coating on said second side of said first electrode;
f. a first controllable valve under control of said controller, wherein said first controllable valve can operate in either of two mutually-exclusive states under control of said controller, and said first controllable valve comprises:
i. first and second inlets, and
ii. an outlet, wherein in a first operating state, said outlet of said first controllable valve is in fluid communication with said second inlet which is in fluid communication with a source of the sample fluid, in a second operating state, said outlet of said first controllable valve is in fluid communication with said first inlet which is in fluid communication with said source of neutral fluid;
g. a first pump, wherein said first pump is a positive displacement pump under control of said controller, an inlet of said first pump is in fluid communication with said outlet of said first controllable valve, and an outlet of said first pump is operatively coupled to said first inlet of said first cavity, and said outlet of said first pump is operatively coupled to said second inlet of said second cavity;
h. a source of regeneration fluid, wherein said regeneration fluid provides for removing said substance adsorbed by said coating on said second side of said first electrode when said regeneration fluid is pumped into said first cavity;
i. a second pump, wherein said second pump is under control of said controller, an inlet of said second pump is in fluid communication with said source of regeneration fluid, and an outlet of said second pump is operatively coupled to said first inlet of said first cavity;
j. at least one second valve, wherein said at least one second valve provides for selectively and mutually-exclusively coupling said outlet of said first pump and said outlet of said second pump to said first inlet of said first cavity;
k. wherein in a first mode of operation, said controller provides for automatically operating said first controllable valve in said first operating state, and provides for automatically operating said first pump, so as to provide for pumping said sample fluid through said first cavity and through said second cavity, either at the same time or during alternate time periods; during said first mode of operation, said controller provides for comparing said change in said frequency difference with a first threshold, and if said change in said frequency difference crosses said first threshold, said controller provides for automatically switching to a second mode of operation;
l. in said second mode of operation, said controller provides for automatically operating said first controllable valve in said second operating state, provides for automatically operating said first pump, and provides for automatically operating said second pump, so as to provide for pumping said regeneration fluid through said first cavity, and so as to provide for pumping said neutral fluid through said second cavity; during said second mode of operation, said controller provides for comparing said change in said frequency difference with a second threshold, and if said change in said frequency difference does not cross said second threshold, said controller provides for automatically terminating said second mode of operation; and
m. said controller provides for automatically resuming said first mode of operation after terminating said second mode of operation.
2. A method of sensing a substance in a sample fluid as recited in
a. automatically commencing a rinse process after completion of step e of
i. automatically pumping said neutral fluid as said first fluid through said first cavity, and detecting said first frequency responsive thereto;
ii. automatically pumping said neutral fluid as said second fluid through said second cavity, and detecting said second frequency responsive thereto; and
b. continuing said rinse process until a change, over a period of time, of said change in said frequency difference, by which said second frequency differs from said first frequency during said rinse process, crosses a third threshold, then automatically terminating said rinse and refresh processes.
3. A method of sensing a substance in a sample fluid as recited in
4. A method of sensing a substance in a sample fluid as recited in
5. A method of sensing a substance in a sample fluid as recited in
6. A method of sensing a substance in a sample fluid as recited in
7. A method of sensing a substance in a sample fluid as recited in
8. A method of sensing a substance in a sample fluid as recited in
9. A method of sensing a substance in a sample fluid as recited in
10. A method of sensing a substance in a sample fluid as recited in
11. A method of sensing a substance in a sample fluid as recited in
12. A method of sensing a substance in a sample fluid as recited in
13. A method of sensing a substance in a sample fluid as recited in
14. A method of sensing a substance in a sample fluid as recited in
a. calculating an amount of said substance adsorbed on said second side of said first electrode responsive to said change in said frequency difference and responsive to stored calibration data; and
b. calculating a total amount or volume of said sample fluid responsive to a duration of time over which said sample fluid was pumped through said first cavity.
15. A method of sensing a substance in a sample fluid as recited in
16. A method of sensing a substance in a sample fluid as recited in
a. filtering at least one anion from said sample fluid with an anion exchanger in chloride form prior to the operation of pumping said sample fluid through said first cavity; or
b. filtering at least one cation from said sample fluid with a cation exchanger in sodium form prior to the operation of pumping said sample fluid through said first cavity.
17. A method of sensing a substance in a sample fluid as recited in
a. monitoring said sample fluid following at least of the operation of filtering at least one anion or the operation of filtering at least one cation to detect either a breakthrough of said anion exchanger or a breakthrough of said cation exchanger;
b. if said breakthrough of said anion exchanger is detected, then pumping a first regenerating fluid through said anion exchanger so as to provide for regenerating said anion exchanger; and
c. if said breakthrough of said cation exchanger is detected, then pumping a second regenerating fluid through said cation exchanger so as to provide for regenerating said cation exchanger.
18. A method of sensing a substance in a sample fluid as recited in
a. if said pH of said sample fluid is greater than a first threshold, then pumping a first generating fluid through a cation exchanger and mixing an output therefrom with said sample fluid, wherein said cation exchanger is in H+ form; or
b. if said pH of said sample fluid crosses a second threshold, then pumping a second generating fluid through an anion exchanger and mixing an output therefrom with said sample fluid, wherein said anion exchanger is in OH− form, wherein said first and second generating fluids are either the same type of fluid or are different types of fluids.
19. A method of sensing a substance in a sample fluid as recited in
20. A method of sensing a substance in a sample fluid as recited in
21. A method of sensing a substance in a sample fluid as recited in
22. A method of sensing a substance in a sample fluid as recited in
a. measuring a temperature of said sample fluid; and
b. either adjusting a flow rate of said positive displacement pump so as to provide for normalizing the flow rate of said sample fluid with respect to said temperature or accounting for an effect of said temperature on a calculation of a flow of said sample fluid through said first cavity.
23. A method of sensing a substance in a sample fluid as recited in
a. prior to the operation of pumping said regeneration fluid, automatically pumping said neutral fluid through said second cavity and measuring a first flow rate of said neutral fluid pumped through said second cavity; and
b. performing the operation of automatically pumping said regeneration fluid through said first cavity at a second flow rate that is substantially the same as said first flow rate.
25. A system for sensing a substance in a sample fluid as recited in
26. A system for sensing a substance in a sample fluid as recited in
27. A system for sensing a substance in a sample fluid as recited in
28. A system for sensing a substance in a sample fluid as recited in
29. A system for sensing a substance in a sample fluid as recited in
30. A system for sensing a substance in a sample fluid as recited in
31. A system for sensing a substance in a sample fluid as recited in
32. A system for sensing a substance in a sample fluid as recited in
33. A system for sensing a substance in a sample fluid as recited in
34. A system for sensing a substance in a sample fluid as recited in
35. A system for sensing a substance in a sample fluid as recited in
36. A system for sensing a substance in a sample fluid as recited in
37. A system for sensing a substance in a sample fluid as recited in
a. calculating an amount of said substance adsorbed on said second side of said first electrode responsive to said change in said frequency difference and responsive to stored calibration data; and
b. calculating a total amount or volume of said sample fluid responsive to a duration of time over which said sample fluid was pumped through said first cavity.
38. A system for sensing a substance in a sample fluid as recited in
39. A system for sensing a substance in a sample fluid as recited in
a. an anion exchange filter in chloride form upstream of said first pump; or
b. a cation exchange filter in sodium form upstream of said first pump.
40. A system for sensing a substance in a sample fluid as recited in
a at least one chemical monitor downstream of a corresponding at least one of said anion exchange filter or said cation exchange filter, wherein said at least one chemical monitor provides for detecting a breakthrough of a corresponding at least one of said anion exchange filter or said cation exchange filter;
b. at least one source of regeneration fluid;
c. at least one controllable valve operatively coupling said at least one source of regeneration fluid to a corresponding at least one of said anion exchange filter or said cation exchange filter; and
d. at least one pump operatively coupling said at least one source of regeneration fluid to a corresponding said at least one controllable valve, wherein said controller or a separate controller operatively coupling said at least one chemical monitor to said at least one controllable valve, provides for controlling said at least one pump and said at least one controllable valve responsive to a corresponding output of said at least one chemical monitor.
41. A system for sensing a substance in a sample fluid as recited in
a. at least one of a cation exchanger and an anion exchanger;
b. at least one source of generating fluid;
c. at least one pump operatively coupling said at least one source of generating fluid to a corresponding at least one of an input port of said cation exchanger or an input port of said anion exchanger, wherein at least one output port of said cation exchanger or said anion exchanger is in fluid communication with said inlet of said first pump.
42. A system for sensing a substance in a sample fluid as recited in
43. A system for sensing a substance in a sample fluid as recited in
44. A system for sensing a substance in a sample fluid as recited in
45. A system for sensing a substance in a sample fluid as recited in
46. A system for sensing a substance in a sample fluid as recited in
a. a first flow sensor for sensing a first rate of flow of said neutral fluid into said second cavity; and
b. a second flow sensor for sensing a second rate of flow of said regeneration fluid into said first cavity, wherein said controller provides for controlling at least one of said first and second pumps so that said second rate of flow is substantially equal to said first rate of flow during at least a portion of said second mode of operation.
48. A method of providing for sensing a substance in a sample fluid as recited in
a. providing for automatically commencing a rinse process after completion of step e of
i. automatically pumping said neutral fluid as said first fluid through said first cavity, and detecting said first frequency responsive thereto;
ii. automatically pumping said neutral fluid as said second fluid through said second cavity, and detecting said second frequency responsive thereto; and
b. providing for continuing said rinse process until a change, over a period of time, of said change in said frequency difference, by which said second frequency differs from said first frequency during said rinse process, crosses a third threshold, then providing for automatically terminating said rinse and refresh processes.
49. A method of providing for sensing a substance in a sample fluid as recited in
50. A method of providing for sensing a substance in a sample fluid as recited in
51. A method of providing for sensing a substance in a sample fluid as recited in
52. A method of providing for sensing a substance in a sample fluid as recited in
53. A method of providing for sensing a substance in a sample fluid as recited in
54. A method of providing for sensing a substance in a sample fluid as recited in
55. A method of providing for sensing a substance in a sample fluid as recited in
56. A method of providing for sensing a substance in a sample fluid as recited in
57. A method of providing for sensing a substance in a sample fluid as recited in
58. A method of providing for sensing a substance in a sample fluid as recited in
59. A method of providing for sensing a substance in a sample fluid as recited in
60. A method of providing for sensing a substance in a sample fluid as recited in
a. providing for calculating an amount of said substance adsorbed on said second side of said first electrode responsive to said change in said frequency difference and responsive to stored calibration data; and
b. providing for calculating a total amount or volume of said sample fluid responsive to a duration of time over which said sample fluid was pumped through said first cavity.
61. A method of providing for sensing a substance in a sample fluid as recited in
62. A method of providing for sensing a substance in a sample fluid as recited in
a. providing for filtering at least one anion from said sample fluid with an anion exchanger in chloride form prior to the operation of pumping said sample fluid through said first cavity; or
b. providing for filtering at least one cation from said sample fluid with a cation exchanger in sodium form prior to the operation of pumping said sample fluid through said first cavity.
63. A method of providing for sensing a substance in a sample fluid as recited in
a. providing for monitoring said sample fluid following at least of the operation of filtering at least one anion or the operation of filtering at least one cation to detect either a breakthrough of said anion exchanger or a breakthrough of said cation exchanger;
b. if said breakthrough of said anion exchanger is detected, then providing for pumping a first regenerating fluid through said anion exchanger so as to provide for regenerating said anion exchanger; and
c. if said breakthrough of said cation exchanger is detected, then providing for pumping a second regenerating fluid through said cation exchanger so as to provide for regenerating said cation exchanger.
64. A method of providing for sensing a substance in a sample fluid as recited in
a. if said pH of said sample fluid is greater than a first threshold, then providing for pumping a first generating fluid through a cation exchanger and mixing an output therefrom with said sample fluid, wherein said cation exchanger is in H+ form; or
b. if said pH of said sample fluid crosses a second threshold, then providing for pumping a second generating fluid through an anion exchanger and mixing an output therefrom with said sample fluid, wherein said anion exchanger is in OH− form, wherein said first and second generating fluids are either the same type of fluid or are different types of fluids.
65. A method of providing for sensing a substance in a sample fluid as recited in
66. A method of providing for sensing a substance in a sample fluid as recited in
67. A method of providing for sensing a substance in a sample fluid as recited in
68. A method of providing for sensing a substance in a sample fluid as recited in
a. providing for measuring a temperature of said sample fluid; and
b. providing for either adjusting a flow rate of said positive displacement pump so as to provide for normalizing the flow rate of said sample fluid with respect to said temperature or accounting for an effect of said temperature on a calculation of a flow of said sample fluid through said first cavity.
69. A method of providing for sensing a substance in a sample fluid as recited in
a. prior to the operation of pumping said regeneration fluid, providing for automatically pumping said neutral fluid through said second cavity and measuring a first flow rate of said neutral fluid pumped through said second cavity; and
b. providing for performing the operation of automatically pumping said regeneration fluid through said first cavity at a second flow rate that is substantially the same as said first flow rate.
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The instant application claims the benefit of prior U.S. Provisional Application Ser. No. 62/241,196 filed on 14 Oct. 2015.
In the accompanying drawings:
Referring to
The first electrode 26 of the active sensor 16 and the third electrode 30 of the reference sensor 18 are each respectively coated with an adsorption layer 34.1, 34.2 of adsorption material 34′ that—absent any further modification thereof—provides for preferentially adsorbing the adsorbable substance 12 when the adsorbable substance 12 comes into contact with the adsorption material 34′. The adsorption layer 34.2 of the reference sensor 18 is either further coated with a blocking layer 36 of an associated blocking substance 36′, or treated with the blocking substance 36′, wherein the blocking substance 36′ binds to the adsorption binding sites of the associated adsorption material 34′ of the associated adsorption layer 34.2 of the reference sensor 18 so as to prevent any subsequent adsorption of an adsorbable substance 12 that might be contained in the sample fluid 14 when exposed thereto.
The active sensor 16 incorporates a first cavity 38 adjacent to and bounded in part by the adsorption layer 34.1 of the first electrode 26. Similarly, the reference sensor 18 incorporates a second cavity 40 adjacent to and bounded in part by the blocking layer 36 of the third electrode 30. The sample fluid 14 is pumped through both the first 38 and second 40 cavities at approximately the same rates, wherein the flow rate through the first cavity 38 is either predetermined (for example, by using an associated positive displacement pump) or measured, so as to provide for determining the total volume of sample fluid 14 that flows through the first cavity 38 and is subject to sensing by the active sensor 16. The flow rate of the sample fluid 14 may be corrected for temperature by also measuring the temperature thereof.
The active 20.1 and reference 20.2 piezoelectric resonators are resonated by at least one associated resonator drive circuit 42, each comprising an associated oscillator, the frequency of which is inherently controlled by the associated active 20.1 or reference 20.2 piezoelectric resonator to which the oscillator is connected. In one embodiment, as resonator drive circuit 42 is shared between the active 20.1 and reference 20.2 piezoelectric resonators, and is alternately repetitively switched therebetween, so as to drive each of the active 20.1 and reference 20.2 piezoelectric resonators at alternate, mutually-exclusive intervals of time. In another embodiment, a separate, distinct resonator drive circuit 42.1, 42.2 is associated with each of the active 20.1 and reference 20.2 piezoelectric resonators, wherein the resonator drive circuits 42.1, 42.2 are either alternately activated or coupled, and deactivated or decoupled, during alternate period of times, particularly for embodiments for which the active 20.1 and reference 20.2 piezoelectric resonators comprise different portions of a single continuous piece of associated piezoelectric material 24; or simultaneously activated and coupled to the active 20.1 and reference 20.2 piezoelectric resonators, respectively, for example, for embodiments for which the active 20.1 and reference 20.2 piezoelectric resonators comprise different distinct pieces of piezoelectric material 24.
The resonant frequency of the each of the active 20.1 and reference 20.2 piezoelectric resonators is dependent primarily upon the respective masses thereof, but can also be affected by the temperature, density and viscosity of the associated sample fluid 14 within the first 38 and second 40 cavities. Following initial manufacture, the masses of the underlying associated portions of either the associated continuous piezoelectric wafer 22, or the corresponding separate active 22.1 and reference 22.1 piezoelectric wafers, and the corresponding pairs of first 26 and second 28 electrodes and third 30 and fourth 32 electrodes, together with the associated adsorption layers 34.1, 34.2, would typically be substantially the same, so as to provide for the active 20.1 and reference 20.2 piezoelectric resonators to have substantially the same nominal resonant frequency prior to introduction of the blocking layer 36 to the reference piezoelectric resonator 20.2, the latter of which acts to increase the mass thereof and lower the corresponding resonant frequency relative to that of the virgin active piezoelectric resonator 20.1. As the sample fluid 14 containing detectable amounts of the adsorbable substance 12 is pumped through the first 38 and second 40 cavities, the flow through the first cavity 38 so as to provide sufficient residence time therewithin for the adsorbable substance 12 therewithin to become adsorbed by the adsorption layer 34.1 on the first electrode 26 of the active sensor 16, thereby increasing the mass thereof, and as a result, decreasing the associated resonant frequency of the active piezoelectric resonator 20.1. If either the active 16 or reference 18 sensor was constructed as a corresponding quartz-crystal resonator 20′, this would be commonly referred to as a quartz-crystal microbalance (QCM), which provides for measuring the moles, mass or weight of a substance adsorbed on the quart-crystal resonator 20′ thereof, responsive to a change in the resonant frequency thereof.
The change in resonant frequency of an AT-cut quartz-crystal resonator 20′ as a result of an accumulation of mass thereon can be characterized by what is known as the Sauerbrey equation, which treats the mass that is adsorbed by or deposited on the quartz-crystal resonator 20′ as an extension of the thickness of the underlying quartz-crystal material 24′, and which, for a quartz-crystal resonator 20′ exposed to air, is given by:
assuming that the adsorbed or deposited mass is rigid and evenly-distributed, and assuming that the relative change of frequency, i.e. Δf/f0, is less than 2%, wherein Δf is the change in resonant frequency (Hz) of the quartz-crystal resonator 20′ as a result of a Δm change of mass (g) thereof f0 is the nominal resonant frequency (Hz) of the quartz-crystal resonator 20′ prior to the change of mass thereof; A is pizoelectrically active area (cm2) of the quartz-crystal material 24′ of the quartz-crystal resonator 20′ between the first 26 and second 28 electrodes, or between the third 30 and fourth 32 electrodes; ρq is the density of the quartz-crystal material 24′ (ρq=2.648 g/cm3); and μq is the shear modulus of the quartz-crystal material 24′ for an AT-cut crystal (μq=2.947×10−11 g·cm−1·s−2). The change of frequency Δf given by equation (1) is substantially independent of the associated electrode geometry.
If the relative change of frequency is greater than 2%, the change in mass Δm of the quartz-crystal material 24′ of the quartz-crystal resonator 20′ can be determined by what is known as the Z-match method using the following equation
wherein fL is the frequency (Hz) of the mass-loaded quartz-crystal resonator 20′; Nq is a frequency constant for an AT-cut crystal (Ng=1.668×10−13 Hz·Å), and
wherein ρf is the density (g/cm3) of the adsorbed or deposited mass, and μl is the shear modulus (g·cm−1·s−2) of the adsorbed or deposited mass.
For operation of the quartz-crystal resonator 20′ in a liquid—as is the case for the active 20.1 and reference 20.2 piezoelectric resonators —, the change in resonant frequency of the quartz-crystal resonator 20′ as a result of the viscosity of the liquid in contact with the quartz-crystal resonator 20′, i.e. the sample fluid 14, is given by:
wherein ρl is the density of the liquid in contact with the quartz-crystal resonator 20′, and ηl is the viscosity of the liquid in contact with the quartz-crystal resonator 20′. This viscosity- and density-dependent change in frequency, as well an associated temperature-dependent change in frequency—in addition to the effect of the mass of the adsorbable substance 12 or target analyte 12 on the change Δf in resonant frequency—is inherently compensated for by simultaneously measuring the resonant frequencies of the active 20.1 and reference 20.2 piezoelectric resonators with each exposed to a different sample of the same sample fluid 14, and using the frequency difference therebetween to provide a measure of the change of mass of the active piezoelectric resonator 20.1, because the resonant frequencies of both the active 20.1 and reference 20.2 piezoelectric resonators would be similarly perturbed by the effect of temperature, density and viscosity, and the effect of the associated perturbation of each would be cancelled by the differencing of the associated separate resonant frequencies. Similarly, the resonant frequencies of each of the active 20.1 and reference 20.2 piezoelectric resonators can each be affected—and jointly affected—by other artifacts such as vibrations; chemical properties, including ionic strength of, and pH changes in, the sample fluid 14 can elicit or affect an associated frequency response in both the active 20.1 and reference 20.2 piezoelectric resonators that would be inherently compensated for by frequency differencing. Furthermore, the ionic strength of, and pH changes in, the sample fluid 14 can affect the mass of the associated adsorption layer 34.1, 34.2 in both the active 20.1 and reference 20.2 piezoelectric resonators, which in turn elicits associated frequency responses therein that would be inherently compensated for by frequency differencing.
With the sample fluid 14 split and pumped at substantially the same flow rate through both the first 38 and second 40 cavities, both the active 20.1 and reference 20.2 piezoelectric resonators will experience substantially the same effects of temperature, density and viscosity of the sample fluid 14 on the associated resonant frequencies of thereof, so that the difference in resonant frequencies of the active 20.1 and reference 20.2 piezoelectric resonators—referred to as the frequency difference of the active 20.1 and reference 20.2 piezoelectric resonators—will be substantially responsive to the difference in mass thereof. The active 20.1 and reference 20.2 piezoelectric resonators are calibrated to provide a relationship—either tabular or functional—between frequency difference and the corresponding mass or moles of adsorbable substance 12 adsorbed on the adsorption layer 34.1 of the active piezoelectric resonator 20.1, which together with the separately determined total moles, volume or mass of sample fluid 14, can then be used to continuously determine a concentration of the adsorbable substance 12 in the sample fluid 14.
For example,
A controller 46 operatively coupled to the at least one associated resonator drive circuit 42 determines the frequency difference—i.e. the amount by which the resonant frequency of the reference piezoelectric resonator 20.2 exceeds that of the active piezoelectric resonator 20.1—between the active 20.1 and reference 20.2 piezoelectric resonator, and determines the concentration of the adsorbable substance 12 in the sample fluid 14 for display on, or transfer to, an associated display 48 either incorporated with, or remotely located with respect to, the fluid contaminant sensing system 10.
Referring to
For example, for the refresh process having commenced with the fluid contaminant sensing system 10 in the state illustrated in
Referring to
Referring to
For example, for each of the first 26 and third 30 electrodes on the first surface 22′ of the piezoelectric wafer 22, corresponding associated respective conductive paths 26.1 and 30.1 are also deposited on the first surface 22′ of the piezoelectric wafer 22, each extending from the associated electrode 26, 30 to the periphery 62 of the piezoelectric wafer 22. Similarly, for each of the second 28 and fourth 32 electrodes on the second surface 22″ of the piezoelectric wafer 22, corresponding associated respective conductive paths 28.1 and 32.1 are also deposited on the second surface 22″ of the piezoelectric wafer 22, each extending from the associated electrode 28, 32 to the periphery 62 of the piezoelectric wafer 22.
The conductive paths 26.1, 28.1 from the first 26 and second 28 electrodes on opposing surfaces 22′, 22″ of the piezoelectric wafer 22 are misaligned with respect to one another, for example having a relative included angle of approximately 90 degrees, so as to not act to resonate the portion of the piezoelectric wafer 22 therebetween, and to thereby limit the piezoelectric action of the first 26 and second 28 electrodes to the region of the piezoelectric wafer 22 directly therebetween. Similarly, the conductive paths 30.1, 32.1 from the third 30 and fourth 32 electrodes on opposing surfaces 22′, 22″ of the piezoelectric wafer 22 are misaligned with respect to one another, for example having a relative included angle of approximately 90 degrees, so as to not act to resonate the portion of the piezoelectric wafer 22 therebetween, and to thereby limit the piezoelectric action of the third 30 and fourth 32 electrodes to the region of the piezoelectric wafer 22 directly therebetween, wherein conductive paths 26.1, 28.1 are diametrically opposed with respect to conductive paths 30.1, 32.1 with respect to a plan view of the piezoelectric wafer 22.
For example, in one set of embodiments, the piezoelectric wafer 22 piezoelectric wafer 22 is and AT-cut quartz crystal having a nominal diameter of 0.538 inches (13.67 mm), from International Crystal Manufacturing, and configured to resonate at about 10 MHz—or more generally, in the range of 3 to 50 MHz. The particular diameter is not limiting, nor is the particular resonant frequency, although the resolution of the associated mass or mole measurements is finer (i.e. increases) with increasing resonant frequency.
The electrodes 26, 28, 30 and 32 and the associated conductive paths 26.1, 28.1, 30.1 and 32.1 are formed by depositing associated layers of titanium—for example, each having a thickness of about 100 Å—on the first 22′ and second 22″ surfaces of the piezoelectric wafer 22, and then depositing associated layers of gold—for example, having a thickness of about 1000 Å—on the first 22′ and second 22″ surfaces of the piezoelectric wafer 22 over the associated titanium layers. For example, the various layers may be deposited by vapor deposition of with heating or sputtering of the material to be deposited.
The conductive paths 26.1, 28.1, 30.1 and 32.1 are interconnected to the associated resonator drive circuit 42, 42.1, 42.2 using spring-loaded contact-forming Pogo-pin-style connectors 64—illustrated in
When assembled in the fluid contaminant sensor cell 54, the first 26 and third 30 electrodes of the active 20.1 and reference 20.2 piezoelectric resonators are fluid-exposed electrodes during use thereof, whereas the remaining second 28 and fourth 32 electrodes are fluid-isolated electrodes that, for example, are exposed to air. Referring again to
In accordance with one set of embodiments, in step (206), the fluid-exposed—i.e. top or outer—surface of the third electrode 30 of the reference piezoelectric resonator 20.2 is coated—for example, by spin coating—with an adsorption material 34′ so as to form the corresponding associated adsorption layer 34.2 on the fluid-exposed surface of the third electrode 30 of the reference piezoelectric resonator 20.2.
The adsorption material 34′ comprises one or more types of chemical receptors that can be configured to bind to various types of target analytes 12, for example, an inorganic chemical, compound or element; an organic chemical; or a micro-organism. The adsorption material 34′ may be configured either to selectively bind to an individual target analyte 12, for example, copper; or to non-selectively bind to a group of target analytes 12, for example, heavy-metal cations.
In accordance with a first aspect, the adsorption material 34′ utilized in steps (204) and (206) of process (200) of
For example, in one set of embodiments to non-selectively bind either lead (Pb2+), cadmium (Cd2+), or mercuric ion (Hg2+) in the sample fluid 14, the polyamine adsorption material 34′—e.g. PAH—of each of the adsorption layers 34.1, 34.2 on the first 26 and third 30 electrodes is reacted with EDTA dianhydride. The associated adsorption layer 34.1 on the first electrode 26, —i.e. of the active piezoelectric resonator 20.1—can be refreshed with a solution of 100 mM HCl as the associated regeneration fluid 50—used in a refresh process described more fully hereinbelow—applied thereto in order to cleanse the adsorption layer 34.1 on the first electrode 26 of associated metal ions, so as to restore the active piezoelectric resonator 20.1 to, or near, its initial, virgin resonant frequency.
Following the formation of the adsorption layer 34.2 on the third 30 electrode of the reference piezoelectric resonator 20.2, in step (208), the chemical receptors thereof are blocked, or immobilized, so as to prevent any target analyte 12 in the sample fluid 14 from being adsorbed thereby during operation of the fluid contaminant sensing system 10. For example, if the target analyte 12 is lead (Pb2), then the adsorption layer 34.2 on the third electrode 30 of the reference piezoelectric resonator 20.2 may be blocked, or immobilized, by saturating the chemical receptors of the associated EDTA groups with (Pb2+) ions. As another example, if the target analyte 12 is cadmium (Cd2+), then the adsorption layer 34.2 on the third electrode 30 of the reference piezoelectric resonator 20.2 may be blocked, or immobilized, by saturating the chemical receptors of the associated EDTA groups with Cd2+ ions. As yet another example, if the target analyte 12 is mercuric ion (Hg2+), then the adsorption layer 34.2 on the third electrode 30 of the reference piezoelectric resonator 20.2 may be blocked, or immobilized, by saturating the chemical receptors of the associated EDTA groups with Hg2+ ions. In any of these cases, any one of the associated blocking substances 36′, i.e. Pb2+, Cd2+ or Hg2+ would be effective against blocking the adsorption of any of the remaining ions because the EDTA groups do not act as exchanger.
In accordance with an alternative, second aspect, the adsorption layer 34.1 on the first electrode 26 of the active piezoelectric resonator 20.1 is the same as described hereinabove for the first aspect but the adsorption layer 34.2 on the third electrode 30 of the reference piezoelectric resonator 20.2 is formed without reacting the polyamine adsorption material 34′ with EDTA dianhydride. More particularly, the adsorption material 34′ utilized in step (206) of process (200) of
In accordance with an alternative third aspect to non-selectively bind arsenite, As(III), in the sample fluid 14, in steps (204) and (206) of process (200) of
In accordance with an alternative fourth aspect to selectively bind arsenite, As(III), in the sample fluid 14, in steps (204) and (206) of process (200) of
The candidate bis-thiol-containing compounds are unique by comparison to other thiol-containing compounds in that they do not oxidize to disulfide (—S—S—) under atmospheric or aqueous conditions. In addition to complete As removal in pH neutral water, BDET has been shown to bind As in the presence of competing elements such as Zn, Cd, and Pb, in gold mining effluent, and for the binding of As in soil. BDET is ideally suited for use as the recognition component of an As sensor. In use, BDET derivatized with a carboxylate group, BDET-COOH, will be used as the surface coating on a QCM sensor. Under ambient pH conditions BDET-COOH will bind selectively to As (III) The resonant frequency by the active piezoelectric resonator 20.1 will directly correspond to the As bound on the surface BDET layer. Thus, the concentration of As present in the water being monitored is quantitatively determined.
The associated adsorption layer 34.1 on the first electrode 26, —i.e. of the active piezoelectric resonator 20.1—can be refreshed with a solution of HCL having a pH less than or equal to 4 as the associated regeneration fluid 50—used in a regeneration process described more fully hereinbelow—applied thereto in order to remove the bound arsenic and regenerate the active form of BDET adsorption layer 34.1 on the first electrode 26, so as to restore the active piezoelectric resonator 20.1 to, or near, its initial, virgin resonant frequency. The acid solution is then neutralized and the As(III) ions are filtered in a small As sorbent column before the solution is discharged.
Following the formation of the adsorption layer 34.2 on the third 30 electrode of the reference piezoelectric resonator 20.2, in step (208), the chemical receptors thereof are blocked, or immobilized, by saturating the chemical receptors thereof with arsenite, As(III) ions.
When using a PAH/BDET adsorption material 34′ to selectively bind arsenite, As(III), the fluid contaminant sensing system 10 would utilize a cation filter—for example, comprising a cation exchange column in sodium (Na) form, as described more fully hereinbelow—upstream of the active sensor 16 so as to provide for removing cations from the sample fluid 14 that might otherwise interfere with the measurement by becoming adsorbed on the adsorption layer 34.1 of the active piezoelectric resonator 20.1.
The bonding of BDET to soft metals and metalloids takes place through covalent bond formation between the elements and the two sulfur atoms of the ligand. Thus, there will be no interferences from the binding of anions such as arsenate (As(V)), phosphate, nitrate or others. However, 95% of selenite, H2SeO3 is bound by BDET at pH 5.5. However, selenite can be easily reduced to elemental selenium (E0=+0.74 V) prior to the active sensor 16 with a reduction column by which As(V) is reduced to As(III) by passing the As(V) through a column of zero valence iron(ZVI).
In waters that contain As(V), a column with a reducing agent such as particulate Fe can be inserted upstream in the sample flow. The As(V) (E0=+0.56 V) is reduced to As(III) which is detected by the sensor. When desired, there can be two sample streams and two sensors, with one having the reducing column and one not. The difference between the two will indicate the presence of As(V).
The only interferences possible with BDET are with soft metals such as Cd, Hg, or Pb. In waters that contain cations, a cation exchange resin column in the sodium form is inserted upstream in the sample flow (for example, using ⅛″ (3 mm) tubing at a flow rate of about 0.1 mL/min). The cation resin exchanges any Cd, Hg or Pb with Na that will not bind to the sensor. BDET does not bind Na or other cations from Groups 1 and 2.
In accordance with an alternative fifth aspect to selectively bind hexachlorobenzene in the sample fluid 14, in steps (204) and (206) of process (200) of
Following the formation of the adsorption layer 34.2 on the third 30 electrode of the reference piezoelectric resonator 20.2, in step (208), the above-described template is not removed from the adsorption layer 34.2 on the third 30 electrode so as to provide for the template to act as the blocking substance 36′ of the associated blocking layer 36.
In accordance with an alternative sixth aspect to detect biological substances. Antibodies can easily be produced that are specific for a wide variety of substances both large and small, and accordingly may be used as an associated adsorption material 34′ of the adsorption layer 34.1 on the first electrode 26 of the active piezoelectric resonator 20.1, and of the adsorption layer 34.2 (if used) on the third electrode 30 of the reference piezoelectric resonator 20.2. For example, if monitoring for bacterial contamination, an antibody against any of the proteins on the bacterial surface can be made and applied as the adsorption material 34′ of the associated adsorption layers 34.1, 34.2.
For example, to create a sensor for E. coli, in steps (204) and (206) of process (200) of
The reagent used as the regeneration fluid 50 to refresh the associated adsorption layer 34.1 on the first electrode 26, —i.e. of the active piezoelectric resonator 20.1 —, so as to restore the active piezoelectric resonator 20.1 to, or near, its initial, virgin resonant frequency, will be different for different antibodies depending on the stability range thereof, which would generally be in the range of pH 4 to pH 10. The stability range is supplied by the laboratories that create the antibodies. Mild acid or mild base reagents are the usual choices.
Following the formation of the adsorption layer 34.2 on the third 30 electrode of the reference piezoelectric resonator 20.2, in step (208), the chemical receptors thereof are blocked, or immobilized, using an antigen—for example, and E. coli ghost—as the associated blocking substance 36′.
In accordance with a further alternative aspect, the adsorption layers 34.1, 34.2 may be formed in accordance with the teachings of U.S. Patent Application Publication No. US 2005/0196532 A1 to Waldrop, III et al. that was published on 8 Sep. 2005, which is incorporated by reference herein in its entirety.
Referring to
More particularly, a lower side of a first O-ring 78.1 provides for sealing against the first surface 22′ of the piezoelectric wafer 22 proximate to, and concentric with, the first surface portion 20.1′ of the active piezoelectric resonator 20.1, and therefore concentric with the associated first electrode 26, and an upper side of the first O-ring 78.1 provides for sealing against a corresponding first O-ring groove 82.1 in the top block 74, so as to provide for defining a first cavity 38 of the active sensor 16, wherein the first cavity 38 is bounded by the coated fluid-exposed first electrode 26, the first O-ring 78.1, and a corresponding bottom surface portion 74.1 of the top block 74. Furthermore, the first O-ring 78.1 is shaped, e.g. circular, and the inside dimension, e.g. diameter, thereof is sized, so that when the fluid contaminant sensor cell 54 is assembled, the first O-ring 78.1 substantially adjoins the periphery of the first electrode 26.
An upper side of a second O-ring 78.2 provides for sealing against the second surface 22′ of the piezoelectric wafer 22 proximate to, and concentric with, the second surface portion 20.1″ of the active piezoelectric resonator 20.1, and therefore concentric with the associated second electrode 28, and a lower side of the second O-ring 78.2 provides for sealing against a corresponding second O-ring groove 82.2 in the bottom block 76, so as to provide for opposing and balancing the forces applied to the piezoelectric wafer 22 by the first O-ring 78.1 as necessary to seal the periphery of the first cavity 38, which therefore provides for enabling the piezoelectric wafer 22 to float between the first 78.1 and second 78.2 O-rings. Furthermore, the second O-ring 78.2 is shaped, e.g. circular, and the inside dimension, e.g. diameter, thereof is sized, so that when the fluid contaminant sensor cell 54 is assembled, the second O-ring 78.2 substantially adjoins the periphery of the second electrode 28.
A lower side of a third O-ring 80.1 provides for sealing against the first surface 22′ of the piezoelectric wafer 22 proximate to, and concentric with, the third surface portion 20.2′ of the reference piezoelectric resonator 20.2, and therefore concentric with the associated third electrode 30, and an upper second side of the third O-ring 80.1 provides for sealing against a corresponding third O-ring groove 82.3 in the top block 74, so as to provide for defining a second cavity 40 of the reference sensor 18, wherein the second cavity 40 is bounded by the coated fluid-exposed third electrode 30, the third O-ring 80.1, and a corresponding bottom surface portion 74.2 of the top block 74. Furthermore, the third O-ring 80.1 is shaped, e.g. circular, and the inside dimension, e.g. diameter, thereof is sized, so that when the fluid contaminant sensor cell 54 is assembled, the third O-ring 80.1 substantially adjoins the periphery of the third electrode 30.
An upper side of a fourth O-ring 80.2 provides for sealing against the second surface 22′ of the piezoelectric wafer 22 proximate to, and concentric with, the fourth surface portion 20.2″ of the reference piezoelectric resonator 20.2, and therefore concentric with the associated fourth electrode 32, and a lower second side of the fourth O-ring 80.2 provides for sealing against a corresponding fourth O-ring groove 82.4 in the bottom block 76, so as to provide for opposing and balancing the forces applied to the piezoelectric wafer 22 by the third O-ring 80.1 as necessary to seal the periphery of the second cavity 40, which therefore provides for enabling the piezoelectric wafer 22 to float between the third 80.1 and fourth 80.2 O-rings. Furthermore, the fourth O-ring 80.2 is shaped, e.g. circular, and the inside dimension, e.g. diameter, thereof is sized, so that when the fluid contaminant sensor cell 54 is assembled, the fourth O-ring 80.2 substantially adjoins the periphery of the fourth electrode 32.
The top 74 and bottom 76 blocks are keyed to one another, with a key protrusion 84 from the top block 74 that mates with a key recess 86 in the bottom block 76, so as to provide for maintaining the concentricity of the first 82.1 and second 82.2 O-ring grooves with respect to one another, and so as to provide for maintaining the concentricity of the third 82.3 and fourth 82.4 O-ring grooves with respect to one another, and which prevents the top block 84 from either translating or rotating relative to the bottom block 76.
The first cavity 38 incorporates a first inlet 38.1 and a first outlet 38.2, wherein the first inlet 38.1 is in fluid communication with a first inlet port 88 on a first side 90 of the top block 74, and the first outlet 38.2 is in fluid communication with a first outlet port 92 on a second side 94 of the top block 74, wherein the first 90 and second 94 sides of the top block 74 are adjacent to one another, and during operation of the fluid contaminant sensor cell 54, a fluid pumped into the first inlet port 88 flows into the first cavity 38 through the first inlet 38.1 thereof, through the first cavity 38, and, following interaction with the active piezoelectric resonator 20.1, is then discharged from the first cavity 38 via the first outlet 38.2 of the first cavity 38, to, and then out of, the first outlet port 92 of the top block 74. Similarly, the second cavity 40 incorporates a second inlet 40.1 and a second outlet 40.2, wherein the second inlet 40.1 is in fluid communication with a second inlet port 96 on a third side 98 of the top block 74, and the second outlet 40.2 is in fluid communication with a second outlet port 100 on a fourth side 102 of the top block 74, wherein the third 98 and fourth 102 sides of the top block 74 are adjacent to one another, and during operation of the fluid contaminant sensor cell 54, a fluid pumped into the second inlet port 96 flows into the second cavity 40 via the second inlet 40.1 thereof, through the first cavity 38, and, following interaction with the reference piezoelectric resonator 20.2, is then discharged from the second cavity 40 via the second outlet 40.2 of second cavity 40, to, and then out of, the second outlet port 100 of the top block 74. For example, in one set of embodiments, the first inlet 88 and outlet 92 ports, and the second inlet 96 and outlet 100 ports are tapped with female pipe threads so as to provide for connection thereto.
Referring to
The piezoelectric wafer 22 is located within a cylindrical well 114 in the top side 76.1 of the bottom block 76, and the piezoelectric wafer 22 is azimuthally located within the cylindrical well 112 by aligning a registration mark on the piezoelectric wafer 22 (not illustrated), with a corresponding registration mark on the periphery of the cylindrical well 112, which together provides for concentrically aligning the active 20.1 and reference 20.2 piezoelectric resonators with the corresponding O-ring groove 82.1, 82.2, 82.3, 82.4.
Referring to
The top 74 and bottom 76 blocks are held together with fasteners (not illustrated) through two sets of holes 120 in the top 74 and bottom 76 blocks, for example, counter-bored holes 120′ in each of the top 74 and bottom 76 blocks that each cooperate with an associated machine screw and nut so that are each recessed below the top and bottom outer surfaces of the fluid contaminant sensor cell 54 when the fluid contaminant sensor cell 54 is assembled. Alternatively, one set of holes 118 in either the top 74 or bottom 76 blocks could be tapped, or the top 74 and bottom 76 blocks could be held together by some other means, for example, by clamping. Upon assembly of the fluid contaminant sensor cell 54, the pairs 78, 80 of O-rings 78.1, 78.2 and 80.1, 80.2 that sandwich the piezoelectric wafer 22 are compressed between the top 74 and bottom 76 blocks and the piezoelectric wafer 22 so as to seal the peripheries of the first 38 and second 40 cavities, so as to provide for any subsequently-contained fluid to interact primarily with the fluid-exposed-electrodes 26, 30 of the active 20.1 and reference 20.2 piezoelectric resonators, and not other portions of the associated piezoelectric wafer 22. Furthermore, upon assembly of the fluid contaminant sensor cell 54, the contact-forming Pogo-pin-style connectors 64 contact the corresponding conductive paths 26.1, 28.1, 30.1, 32.1 of the of the first 26 and second 28 electrodes and the third 30 and fourth 32 electrodes, so as to provide for external connection to the associated resonator drive circuit 42, 42.1, 42.2.
Although the above-described fluid contaminant sensor cell 54 incorporates a single piezoelectric wafer 22 incorporating active 20.1 and reference 20.2 piezoelectric resonators, it should be understood that separate piezoelectric wafers 22.1, 22.2 incorporating separate active 22.1 and reference 22.1 piezoelectric wafers could alternatively be incorporated in the fluid contaminant sensor cell 54, or separated fluid contaminant sensor cells 54—each constructed similarly to the above-described fluid contaminant sensor cell 54—could be used with separate piezoelectric wafers 22.1, 22.2, with one fluid contaminant sensor cell 54 incorporating the active piezoelectric resonator 20.1, and the other fluid contaminant sensor cell 54 incorporating the reference piezoelectric resonator 20.2.
Referring again to
The first inlet port 88 of the fluid contaminant sensor cell 54, associated with the active sensor 16, is operatively coupled through a second flow sensor 134 to an outlet port 136.3 of a second controllable three-way valve 136 having first 136.1 and second 136.2 inlet ports, wherein the first inlet port 136.1 of the second controllable three-way valve 136 is operatively coupled to the output of the first pump 124, and the second inlet port 136.2 of the second controllable three-way valve 136 is operatively coupled to the output of a second pump 138—for example, a controllable-flow-rate positive displacement pump —, the input of which is operatively coupled to a source of regeneration fluid 50 that is used to refresh the adsorption layer 34.1 of the active piezoelectric resonator 20.1, as described more fully hereinbelow. The first outlet port 92 of the fluid contaminant sensor cell 54, associated with the active sensor 16, is operatively coupled to an inlet port 142.3 of a third controllable three-way valve 142 having first 142.1 and second 142.2 outlet ports, wherein a first outlet port 142.1 of the third controllable three-way valve 142 is operatively coupled to the sample discharge line or tank 132, and the second outlet port 142.2 of the third controllable three-way valve 142 is operatively coupled to a separate waste tank 144 for subsequent disposal.
The first 126, second 136 and third 142 controllable three-way valves are under control of a controller 46, the latter which together provide for controlling the operating states thereof. The controller 46, in cooperation with an associated memory 46.1, provides for controlling the operation of the associated active 16 and reference 18 sensors and for processing the outputs therefrom. In a first operating state 126′ of the first controllable three-way valve 126, the first inlet port 126.1 thereof is operatively coupled to the outlet port 126.3 thereof, so as to provide for the first pump 124—under control of the controller 46—to receive and pump the fluid to be sensed 128 both to the first inlet port 136.1 of the second controllable three-way valve 136, and through the first flow sensor 122 to the reference sensor 18. In a second operating state 126″ of the first controllable three-way valve 126, the second inlet port 126.2 thereof is operatively coupled to the outlet port 126.3 thereof, so as to provide for the first pump 124 to receive and pump the neutral fluid 52, 52′ both to the first inlet port 136.1 of the second controllable three-way valve 136, and through the first flow sensor 122 to the reference sensor 18.
In a first operating state 136′ of the second controllable three-way valve 136, the first inlet port 136.1 thereof is operatively coupled to the outlet port 136.3 thereof, so as to provide for the either the fluid to be sensed 128 or the neutral fluid 52, 52′—depending upon the operating state 126′, 126″ of the first controllable three-way valve 126—to be pumped by the first pump 124 through the second flow sensor 134 and into the first inlet port 88 of the fluid contaminant sensor cell 54, associated with the active sensor 16. In a second operating state 136″ of the second controllable three-way valve 136, the second inlet port 136.2 thereof is operatively coupled to the outlet port 136.3 thereof, so as to provide for the regeneration fluid 50 to be pumped by the second pump 138—under control of the controller 46—through the second flow sensor 134 and into the first inlet port 88 of the fluid contaminant sensor cell 54, associated with the active sensor 16.
In a first operating state 142′ of the third controllable three-way valve 142, the first outlet port 142.1 thereof is operatively coupled to the inlet port 142.3 thereof, so as to provide for discharging the fluid from the first outlet port 92 of the fluid contaminant sensor cell 54 to the sample discharge line or tank 132. In a second operating state 142″ of the third controllable three-way valve 142, the second outlet port 142.2 thereof is operatively coupled to the inlet port 142.3 thereof, so as to provide for discharging the fluid from the first outlet port 92 of the fluid contaminant sensor cell 54 to the waste tank 144 for subsequent disposal, for example, so as to provide for safely disposing a potentially hazardous regeneration fluid 50.
The actuation and flow rate of the second pump 138, and at least the actuation of the first pump 124, are under control of the controller 46. For example, in one set of embodiments, the first 124 and second 138 pumps are each implemented as an adjustable-flow-rate diaphragm pump, for example, an MP-6 micro pump manufactured by Bartels Mikrotechnik.
The first pump 124 provides for a known and controllable flow rate of the sample fluid 14 through the active sensor 16 so as to provide for determining therefrom the total amount of sample fluid 14 pumped therethrough within a given period of time. The concentration of target analyte 12 in the sample fluid 14 can then be calculated from the ratio of the mass or moles of target analyte 12 measured by the active sensor 16, divided by the total amount of sample fluid 14 inferred from the period of time over which the sample fluid 14 was pumped through the active sensor 16 given the known flow rate of the first pump 124.
The second pump 138 provides for pumping the regeneration fluid 50 through the active sensor 16 at a known and controllable flow rate, wherein the flow rate can be adjusted so that the flow rate of the regeneration fluid 50 through the active sensor 16 is the same as the flow rate of the neutral fluid 52 through the reference sensor 18 during a below-described process to refresh the adsorption layer 34.1 of the active sensor 16.
The fluid contaminant sensing system 10 further incorporates a temperature sensor 146 that provides for sensing the temperature of the fluid pumped by the first pump 124—for example, discharged therefrom—to the active 16 or reference 18 sensors, and which is operatively coupled to the controller 46 so as to communicate a signal responsive to that temperature to the controller 46. For example, in one set of embodiments, the temperature sensor 146 comprises a Dallas Temperature sensor DS18B20. The first 122 and second 134 flow sensors are also operatively coupled to the controller 46 so as to provide for communication associated flow rate signals QR, QA thereto, wherein the first flow sensor 122 communicates a measure of the flow rate QR through the reference sensor 18 to the controller 46, and second flow sensor 134 communicates a measure of the flow rate QA through the active sensor 16.
The active 20.1 and reference 20.2 piezoelectric resonators are used in an associated at least one resonator drive circuit 42, 42.1, 42.2 to inherently control the resonant frequency of an associated at least one oscillator 148, 148.1, 148.2 to be the resonant frequency of the associated active 20.1 and reference 20.2 piezoelectric resonator. Referring also to
For example, as illustrated in
Alternatively, the second 28 and fourth 30 electrodes of the active 20.1 and reference 20.2 piezoelectric resonators may be interconnected, or utilize a common electrode—i.e. where the second 28 and fourth 32 electrodes are different portions of an underlying continuous electrode, —either within the fluid contaminant sensor cell 54, or external thereto, with the junction therebetween, or the common electrode, connected to the XIN terminal of the SN74LVC1404 154, and the remaining first 26 and third 30 electrodes connected to first single-pole-double-throw (SPST) switch 150 as described hereinabove, the latter of which is used exclusively to alternate between the active 20.1 and reference 20.2 piezoelectric resonators under control of the controller 46, as described hereinabove.
Yet further alternatively, either single-pole-double-throw (SPDT) switch 150, 152 could be implemented with a corresponding pair of single-pole-single-throw (SPST) switches.
Yet further alternatively, referring to
Unless in cooperation with active 26 and reference 18 sensors implemented with separate active 22.1 and reference 22.1 piezoelectric wafers that are mechanically isolated from one another, the resonator drive circuit 42, 42.1, 42.2 and associated first 150 and second 152 single-pole-double-throw (SPDT) switches, or single-pole-single-throw (SPST) switches 158.1, 158.2, are operated in one of two operating states 42′, 42″. In the first operating state 42′ of the resonator drive circuit 42, 42.1, 42.2, the pole 150.3 of the first single-pole-double-throw (SPDT) switch 150 is operatively coupled to the first switch contact 150.1 thereof, and the pole 152.3 of the second single-pole-double-throw (SPDT) switch 152 is operatively coupled to the first switch contact 152.1 thereof; or the first single-pole-single-throw (SPST) switch 158.1 is closed (or the associated resonator drive circuit 42.1 is activated), and the second single-pole-single-throw (SPST) switch 158.2 is open (or the associated resonator drive circuit 42.2 is deactivated); depending upon the configuration of the resonator drive circuit 42, 42.1, 42.2, so as to provide for the resonator drive circuit 42, 42.1, 42.2 to drive and oscillate the active piezoelectric resonator 20.1 at its resonant frequency. Similarly, in the second operating state 42″ of the resonator drive circuit 42, 42.1, 42.2, the pole 150.3 of the first single-pole-double-throw (SPDT) switch 150 is operatively coupled to the second switch contact 150.2 thereof, and the pole 152.3 of the second single-pole-double-throw (SPDT) switch 152 is operatively coupled to the second switch contact 152.2 thereof; or the first single-pole-single-throw (SPST) switch 158.1 is open (or the associated resonator drive circuit 42.1 is deactivated), and the second single-pole-single-throw (SPST) switch 158.2 is closed (or the associated resonator drive circuit 42.2 is activated); depending upon the configuration of the resonator drive circuit 42, 42.1, 42.2, so as to provide for the resonator drive circuit 42, 42.1, 42.2 to drive and oscillate the reference piezoelectric resonator 20.2 at its resonant frequency.
Yet further alternatively, the resonator drive circuit(s) 42, 42.1, 42.2, associated switches 150, 152, 158.1, 158.2, and an associated frequency counter can be implemented with an associated application specific integrated circuit (ASIC).
Yet further alternatively, the active 20.1 and reference 20.2 piezoelectric resonators could each be driven in accordance with the teachings of U.S. Pat. No. 6,169,459 for an active-bridge oscillator, which is incorporated by reference herein in its entirety.
In accordance with one set of embodiments, the fluid contaminant sensing system 10 is implemented as what is referred to as a monitor comprising a monitor base unit that cooperates with one or more fluid contaminant sensor cells 54, with each of a plurality of fluid contaminant sensor cells 54 configured to sense a different target analyte 12. The monitor base unit comprises at least one plug-in receptacle for an associated fluid contaminant sensor cells 54; a power supply (not illustrated); and the associated hardware illustrated in
For example, in one set of embodiments, the controller 46 comprises a single-board computer, for example, including, but not limited to, an Arduino or Raspberry Pi micro-computer. For example, in one embodiment, the controller 46 is implemented with a Teensy 3.2 USB Development Board.
The controller 46 may incorporate one or more associated communication buses or ports operating in accordance with one or more associated communication interfaces, for example, inter alia, USB, i2c, SPI, RS232 or RS485, for communicating with the other electronic or electronically-controlled components of the fluid contaminant sensing system 10, so as to provide for the associated monitor to incorporate a modular architecture. In one set of embodiments, the controller 46 comprises a flash-based microcontroller containing routines for reading from the sensor interface and sensor buses, either storing the associated sensor measurement(s) for local processing or display, or transmitting the associated sensor measurement(s) to a remote display, storage or control system 160 for subsequent use. For example, the associated sensor measurement(s) may be transmitted to the remote display, storage or control system 160 using either a cabled (e.g. wired or fiber optic) or wireless connection, either directly or via a network interface, for example, via an Ethernet connection or the Internet; or using a portable or removable storage device, for example, that might incorporate a USB interface to communication with the controller 46.
Referring to
In step (902) of the monitor control process (900), the controller 46 sets the toggle interval, i.e. the period of time over which one of the active 20.1 and reference 20.2 piezoelectric resonators, whichever is activated, remains activated before the other of the active 20.1 and reference 20.2 piezoelectric resonators is activated, in an alternating fashion. For example, in one embodiment, the toggle interval is about 4 seconds. The resonant frequencies can be measured by counting the cycles of oscillation of the associated oscillator 148, in which case, a relatively longer toggle interval would provide for counting more cycles, which provides for a relatively higher resolution. For example, at least a one second toggle interval would provide for a 1 Hz resolution of a 10 MHz signal, with some additional time needed in the toggle interval to allow for stabilization. A lower frequency would require a greater amount of time for the same resolution, and vice versa. In accordance with a first embodiment, the toggling between the active 20.1 and reference 20.2 piezoelectric resonators is under direct, i.e. software, control of the controller 46, using a digital output signal therefrom. In accordance with a second embodiment, the toggling between the active 20.1 and reference 20.2 piezoelectric resonators may alternatively be facilitated with an Analog Devices ADG752 analog switch In accordance with a third embodiment, the toggling between the active 20.1 and reference 20.2 piezoelectric resonators is controlled by the above-described application specific integrated circuit (ASIC).
In step (904) of the monitor control process (900), the controller 46 sets the associated control state of the associated first 126, second 136 and third 142 controllable three-way valves, the first 124 and second 138 pumps, and the first 150 and second 152 single-pole-double-throw (SPDT) switches, or single-pole-single-throw (SPST) switches 158.1, 158.2 depending upon the embodiment, and also controls the associated sequencing of the control states of, and depending upon, the associated calibration, sensing, refresh, preconditioning or pH control process. For example,
In step (906) of the monitor control process (900), the first 126, second 136 and third 142 controllable three-way valves are controlled, i.e. set to the associated operating state, in accordance with the associated control state, as described more fully hereinbelow.
In step (908) of the monitor control process (900), the activations, and possibly the flow rates, of the first 124 and second 138 pumps are controlled in accordance with the associated control state, as described more fully hereinbelow.
In step (910) of the monitor control process (900), depending upon the toggling state of the active 20.1 and reference 20.2 piezoelectric resonators, either the active 20.1 of reference 20.2 piezoelectric resonators—mutually exclusively if using a shared, single piezoelectric wafer 22—are activated so as to resonate the associated active 20.1 or reference 20.2 piezoelectric resonator. For example, in one set of embodiments, for a shared resonator drive circuit 42, the above described first 150 and second 152 single-pole-double-throw (SPDT) switches are switched to provide for switching either the first 26 and second 28 electrodes, or the third 30 and fourth 32 electrodes, to the resonator drive circuit 42 responsive to a toggle signal from the ARM processor of the Teensy 3.2 USB Development Board, depending upon the state—i.e. 0 or 1 of an associated I/O pin—of the toggle signal, wherein the toggle signal is held at a particular state for the above-describe toggle interval before switching to the other state for the same toggle interval, and then repeating the toggling process to repetitively alternate between activation of the active 20.1 and reference 20.2 piezoelectric resonators, wherein the associated toggle interval of the toggle signal from the ARM is programmable or controllable.
Then, in step (912) of the monitor control process (900), the resulting resonant frequency of the activated active 20.1 or reference 20.2 piezoelectric resonator is measured. For example, in one set of embodiments, the output from the oscillator 148, 148.1, 148.2 is operatively coupled to the ARM chip embedded on the Teensy 3.2 USB Development Board, which in turn measures the associated frequency, for example, by measuring the period of time associated with a predetermined number of cycles of the oscillator output signal (for example, using associated Arduino software installed on the Teensy 3.2 USB Development Board). For example, for Frequency counting there is an in-built function in the software “FreqCount.read” which automatically counts the number of peaks of the signal in one second (Counts/sec). This number (Frequency) is placed in a variable and displayed. The frequency counter determines the frequency of each of the active 20.1 and reference 20.2 piezoelectric resonators. Alternatively, the resonant frequencies of the active 20.1 and reference 20.2 piezoelectric resonators may be measured with a commercially-available frequency counter, for example, a Hewlett Packard HP 5386A Frequency Counter.
In step (914) of the monitor control process (900), the frequency difference between the resonant frequencies of the active 20.1 and reference 20.2 piezoelectric resonators, so as to provide for determining the mass or moles of the target analyte 12 with inherent compensation for the effects of the temperature, density and viscosity of the sample fluid 14.
In step (916) of the monitor control process (900), the controller 46 provides for measuring the temperature of the sample fluid 14 is measured with an associated temperature sensor 146, for use in normalizing the associated liquid volume of the sample fluid 14 that has flowed through the active sensor 16, when calculating the concentration of the target analyte 12 in the sample fluid 14. For example, in one set of embodiments, a Dallas Temperature sensor DS18B20 inputs the temperature signal to the ARM chip embedded on the Teensy 3.2 USB Development Board, or a similar microcontroller, for example, that would be associated with each fluid contaminant sensor cell 54. For example, the fluid contaminant sensing system 10 would also incorporate a relatively more powerful single board computer, for example, a Raspberry pi, provides for communicating with the individual microcomputers associated with each fluid contaminant sensor cell 54, and provides for handling associated data processing for the control and reporting functions, external communications, and the user interface. As used herein, the term controller 46 is not limited to a single computer or processor, but is intended to represent one or more computers or processors as necessary to carry out the associated functionality, regardless of the particular data processing, control and communications architecture.
In step (918) of the monitor control process (900), the mass or moles of the target analyte 12 adsorbed by the adsorption layer 34.1 of the first electrode 26 of the active sensor 16 is determined responsive to the frequency difference from step (916), and responsive to associated calibration data, for example, either stored in the memory 46.1 of the controller 46, or stored on an associated EEPROM 162 that is operatively coupled to the controller 46. In accordance with one set of embodiments, the EEPROM 162 is incorporated in or with the fluid contaminant sensor cell 54, so as to provide for the inherently linking the associated calibration data with the fluid contaminant sensor cell 54 to which that calibration applies. For example, the calibration data includes a table or parameters of a mathematical model that provides for defining a functional relationship of the mass or moles of the target analyte 12 adsorbed by the adsorption layer 34.1 of the first electrode 26 of the active sensor 16 as a function of either the associated resulting resonant frequency, or the associated frequency difference with respect to the corresponding resonant frequency of the associated reference sensor 18 when exposed to the same calibration fluid, as described more fully hereinbelow. The calibration data includes an identification of the target analyte 12 to which the calibration table or mathematical-model parameters is associated.
In step (920) of the monitor control process (900), the controller 46 provides for determining the total amount, i.e. moles, mass or volume, of the sensed sample fluid 14 associated with the mass of the target analyte 12 adsorbed by the adsorption layer 34.1 of the first electrode 26 of the active sensor 16, responsive to either the known flow rate of the first pump 124, and the total period of time over which the sample fluid 14 flowed at that flow rate through the first cavity 38 of the active sensor 16, or responsive to a direct measurement of the flow rate using the second flow sensor 134, wherein in either case, the flow rate is corrected to a standard temperature using the temperature measurement from the temperature sensor 146 that was measured in step (916). For example, the concentration might be expressed as parts-per-billion, parts-per-million, or as weight-per-volume, for example, micrograms or nanograms per liter.
In step (922) of the monitor control process (900), the controller 46 provides for determining the concentration of the target analyte 12 as a ratio of the total mass or moles of the target analyte 12 from step (918), divided by the total amount, i.e. moles, mass or volume, of the sensed sample fluid 14 from step (920).
In step (924) of the monitor control process (900), the controller 46 provides for communicating with a remote display, storage or control system 160, so as to provide for transmitting data from the fluid contaminant sensing system 10—for example, measurements and/or associated calculated values, or warning or error messages—thereto, and so as to provide for receiving commands therefrom, for example, to enter a specific control state. For example, the individual microcomputers—which provide for relatively low-level functions, including managing sensor data, pump control, sensor calibration data, switch and valve control, and resonant drive control, preconditioning control, pH measurement, pH control, and provide a generic interface such as Ethernet or USB—would transfer sensor data to the associated single board computer for relatively higher level processing including data aggregation and relatively long-term analyses.
Referring again to
Referring to
Then, referring to
Then, in step (1008), a frequency difference ΔF is determined as the difference between the resonant frequencies FR and FA of the active 20.1 and reference 20.2 piezoelectric resonators, respectively. If, in step (1010), the magnitude of the difference between the frequency difference ΔF and a corresponding initial frequency difference ΔF0—determined prior to commencement of the calibration process (1000)—does not exceed a frequency difference threshold ΔFTHRESHOLD, and if, in step (1012), an iteration count does not exceed a limit, then the calibration process (1000) repeats, beginning with step (1002). For example, in one set of embodiments, the frequency difference threshold ΔFTHRESHOLD is set so that the adsorption layer 34.1 on the first electrode 26 of the active sensor 16 becomes about ⅔ saturated with target analyte 12—as reflected by a corresponding saturation frequency difference ΔFSATURATION, so as to provide for a corresponding effective operating range (EOR) of the active sensor 16. Otherwise, from step (1012), if the iteration limit is exceeded, then, then the calibration process (1000) terminates with an error in step (1014).
For example,
Otherwise, from step (1010), if the difference between the frequency difference ΔF and the corresponding initial frequency difference ΔF0 exceeds the frequency difference threshold ΔFTHRESHOLD, then, in step (1016), in accordance with one aspect, the remaining calibration fluid 164 that was not pumped by the first pump 124 is analyzed to determine the amount of target analyte 12 remaining therein, so as to provide for determining therefrom the amount of target analyte 12 that had been pumped by the first pump 124, which can then be used to determine the amount of target analyte 12 that had been pumped through the active sensor 16 given the above-described measure of the amount of calibration fluid 164 that had been pumped through the active sensor 16. For example, the amount of target analyte 12 remaining in the source container of calibration fluid 164 when commencing step (1016) can be measured using ICP-OES (Inductively Coupled Plasma Optical Emission Spectroscopy) or a similar apparatus.
Alternatively, or additionally, the volume of the calibration fluid 164 (albeit, sans target analyte 12) discharged from the active sensor 16 can be measured, so as to provide for determining the amount of target analyte 12 that had been adsorbed by the adsorption layer 34.1 on the first electrode 26 of the active sensor 16, given the known concentration of the target analyte 12 in the calibration fluid 164 and assuming a particular capture ratio.
Then, in step (1018), given either an associated measurement of the volume of calibration fluid 164 that had been pumped through the active sensor 16, for each corresponding measurement of frequency difference ΔF during the calibration process (1000); or assuming a linear relationship between the frequency difference ΔF and the associated amount of target analyte 12 adsorbed by the adsorption layer 34.1 on the first electrode 26 of the active sensor 16, together with the total mass of target analyte 12 adsorbed during the calibration process (1000); the amount of adsorbed target analyte 12—in molar and/or mass units—is determined for each corresponding value of frequency difference Δf′, and the resulting data is stored as an associated calibration table in the EEPROM 162, over the a range of frequency differences ΔF covering at least the effective operating range (EOR).
Referring again to
Otherwise, from step (1314), if the value of the flow rate difference ΔQ has stabilized over time, so that the flow rate difference ΔQ does not vary by more than an associated threshold from one iteration to the next, then, in step (1320), if the absolute magnitude of the flow rate difference ΔQ is greater than a threshold ΔQMAX, then the first phase refresh process (1300a) terminates in step (1318) with an error as a result of the difference between the flow rates QA, QR through the active 16 and reference 18 sensors being greater than a correctable amount. Otherwise, from step (1320), in step (1322), the value of the flow rate QR through the reference sensor 18 is stored as QR_REF for subsequent use.
Then, in step (1332), a frequency difference ΔF is determined as amount by which the resonant frequency FR of the reference piezoelectric resonator 20.2 exceeds the resonant frequency FA of the active piezoelectric resonator 20.1. Then, in step (1334), if the absolute magnitude of the frequency difference 1F is not less than a corresponding threshold frequency difference ΔF0, then, in step (1336), if an iteration count is less than an associated limit, then the second phase refresh process (1300b) repeats, beginning with step (1324). Otherwise, from step (1336), if the associated iteration count limit has been exceeded, the second phase refresh process (1300b) terminates in step (1338) with an error as a result of an inability to sufficiently cleanse the active sensor 16 of target analyte 12.
Otherwise, from step (1334), if the absolute magnitude of the frequency difference ΔF is less than the corresponding threshold frequency difference ΔF0, —indicating that the active sensor 16 has been sufficiently cleansed of target analyte 12, —then, in step (1340), referring to
If, in step (1346), the value of the frequency difference ΔF has not stabilized over time, or if more than two iterations of steps (1340) through (1344) have not yet been completed, then, in step (1348), if an iteration count is less than an associated limit, then the second phase refresh process (1300b) repeats, beginning with step (1340). Otherwise, from step (1348), if the associated iteration count limit has been exceeded, the second phase refresh process (1300b) terminates in step (1338) with an error because the active sensor 16 is not sufficiently stable. Otherwise, from step (1346), if the value of the frequency difference ΔF has stabilized over time, so that the frequency difference ΔF does not vary by more than an associated threshold from one iteration to the next, then, in step (1350), the second phase refresh process (1300b) terminates without error, with the active sensor 16 refreshed.
Referring to
More particularly, following step (1322) of the first phase refresh process (1300a), in step (1802), referring to
Then, in step (1806), a frequency difference ΔF is determined as amount by which the stored resonant frequency FR_REF of the reference piezoelectric resonator 20.2—stored in step (1322)—exceeds the resonant frequency FA of the active piezoelectric resonator 20.1.
Then, in step (1808), if the absolute magnitude of the frequency difference ΔF is not less than a corresponding threshold frequency difference ΔF0, then, in step (1810), if an iteration count is less than an associated limit, then the alternative second phase refresh process (1800) repeats, beginning with step (1802). Otherwise, from step (1810), if an iteration count is less than an associated limit, the second phase refresh process (1300b) terminates in step (1812) with an error as a result of an inability to sufficiently cleanse the active sensor 16 of target analyte 12.
Otherwise, from step (1808), if the absolute magnitude of the frequency difference ΔF is less than the corresponding threshold frequency difference ΔF0, —indicating that the active sensor 16 has been sufficiently cleansed of target analyte 12, —then, in step (1814), referring to
If, in step (1818), the value of the frequency difference ΔF has not stabilized over time, or if more than two iterations of steps (1814) and (1816) have not yet been completed, then, in step (1820), if an iteration count is less than an associated limit, then the alternative second phase refresh process (1800) repeats, beginning with step (1814). Otherwise, from step (1820), if the associated iteration count limit has been exceeded, the alternative second phase refresh process (1800) terminates in step (1812) with an error because the active sensor 16 is not sufficiently stable. Otherwise, from step (1818), if the value of the frequency difference ΔF has stabilized over time, so that the frequency difference ΔF does not vary by more than an associated threshold from one iteration to the next, then, in step (1822), the alternative second phase refresh process (1800) terminates without error, with the active sensor 16 refreshed.
Referring again to
Then, in step (1906), the sample fluid 14 is optionally preconditioned—for example, by a below-describe preconditioning process (2400)—to mechanically filter the sample fluid 14, and to possibly remove either cations or anions therefrom depending upon the nature of the associated adsorption material 34′ of the adsorption layer 34.1 on the first electrode 26 of the active sensor 16, for example, so as to provide for removing gross contamination, biofouling, organic molecules, cations or anions that might otherwise interfere with the active 16 or reference 18 sensors. Then, in step (1908), the pH of the raw or optionally preconditioned sample fluid 14 is optionally monitored and controlled—for example, by a below-describe pH control process (2700)—for example, so as to provide for adsorption of the target analyte 12 by the adsorption material 34′ of the adsorption layer 34.1 on the first electrode 26 of the active sensor 16, depending upon the particular target analyte 12.
Then, referring to
Then, referring to
Then, in step (1914), a frequency difference ΔF is determined as the difference between the resonant frequencies FR and FA of the active 20.1 and reference 20.2 piezoelectric resonators, respectively. Then, in step (1916), the mass of the target analyte 12 adsorbed by the adsorption layer 34.1 on the first electrode 26 of the active sensor 16 is determined responsive to the difference between the frequency difference ΔF of step (1914) and a corresponding initial frequency difference ΔF0 that is stored after refreshing the active sensor 16, using the calibration data stored on the EEPROM 162.
Then, in step (1918), the total flow of the sample fluid 14 is determined either from 1) a direct measurement from the second flow sensor 134 of the flow rate through the first cavity 38 over an associated period of time; 2) an inference of the flow rate through the first cavity 38 through the first cavity 38 given a predetermined rate of flow of the first pump 124 assuming a given distribution of flow from the first pump 124 to the first 38 and second 40 cavities or from a determination of the distribution of flow using flow rate measurements from the first 122 and second 134 flow sensors; or 3) of the flow rate through the first cavity 38 through the first cavity 38 given a predetermined rate of flow of the first pump 124 if the fluid contaminant sensing system 10 is configured in accordance with alternative topologies of
The, in step (1920), the total volume of sample ΔVSAMPLE that has been pumped through the active sensor 16 since commencement of the most recent sensing cycle is determined responsive to the flow that was measured or determined in step (1918), which is either explicitly or implicitly responsive to the duration of time (t−t0) of the most recent sensing cycle.
Then, in step (1922), the current measurements form the fluid contaminant sensing system 10 are either stored, displayed on a local display 48, or transmitted to a remote display, storage or control system 160.
Then, in step (1924), if the magnitude of the difference between frequency difference ΔF from step (1914) and a corresponding initial frequency difference ΔF0 does not exceed a threshold frequency difference ΔFEOR associated with the effective operating range (EOR) of the active sensor 16, then the particular sensing cycle continues by repeating the continuous sensing process (1900) beginning with step (1906).
Otherwise, from step (1924), in step (1926), the active sensor 16 is regenerated in accordance with the associated refresh processes (1300a) and (1300b or 1800), after which, in step (1926), the values of the total volume VSAMPLE of the sample fluid 14 that had been pumped through the active sensor 16, and the total mass MTARGET of the target analyte 12 in that total volume of the sample fluid 14, are updated to include the corresponding amounts ΔVSAMPLE, ΔMTARGET had been sensed in the most recent sensing cycle, after which a new sensing cycle commences by repeating the continuous sensing process (1900) beginning with step (1904).
Referring to
More particularly, referring to
Referring to
Referring to
Referring also to
Accordingly, following step (2402), in step (2404), if anions are to be filtered, then, in step (2406), the discharge from the mechanical filter 172 is passed through a first controllable three-way valve 174 (controlled by the controller 46) in a first operating state 174′ to an anion filter 176, for example, an anion exchange filter 176′ (also referred to as an anion column) in chloride form, the output of which is discharged through a first chemical monitor 178, and through a second controllable three-way valve 180 (controlled also by the controller 46) in a first operating state 180′, and then discharged from the preconditioner 2500 as the associated preconditioned sample fluid 14′.
In step (2408), the first chemical monitor 178 provides for detecting if there has been a breakthrough from the anion filter 176 of anions from the sample fluid 14 that have been captured by the anion filter 176. More particularly, in one embodiment, the first chemical monitor 180 comprises a quartz-crystal microbalance (QCM) 56 configured as an active piezoelectric resonator 20.1, with the associated adsorption material 34′ of the adsorption layer 34.1 configured as an anion exchanger that is doped, or saturated, with an anion of lower selectivity than the anion to be removed by the anion filter 176, 176′. For example, if As(III) is the cation that may interfere, then the associated adsorption material 34′ of the adsorption layer 34.1 could be doped, or saturated, with chloride, Cl. If As(III) breaks through the anion filter 176, 176′, then the As(III) will displace the Cl on the adsorption layer 34.1 of the associated first electrode 26 of the active piezoelectric resonator 20.1, and the resultant frequency response, i.e. lowered resonant frequency, will indicate the breakthrough.
If, in step (2410), a break-through has been detected by the first chemical monitor 178, then in step (2412), referring also to
Similarly, following step (2402), in step (2404), if cations are to be filtered, then, in step (2414), the discharge from the mechanical filter 172 is passed through a third controllable three-way valve 186 (controlled by the controller 46) in a first operating state 186′ to an anion filter 176, for example, an cation exchange filter 188′ (also referred to as an cation column) in sodium form, the output of which is discharged through a second chemical monitor 190, and through a second controllable three-way valve 192 (controlled also by the controller 46) in a first operating state 192′, and then discharged from the preconditioner 2500 as the associated preconditioned sample fluid 14′.
In step (2416), the second chemical monitor 190 provides for detecting if there has been a breakthrough from the cation filter 188 of anions from the sample fluid 14 that have been captured by the cation filter 188. More particularly, in one embodiment, the first chemical monitor 192 comprises a quartz-crystal microbalance (QCM) 56 configured as an active piezoelectric resonator 20.1, with the associated adsorption material 34′ of the adsorption layer 34.1 configured as a cation exchanger that is doped, or saturated, with an cation of lower selectivity than the cation to be removed by the cation filter 188, 188′, for example, in accordance with the following table of selectivity:
Species
Cation
Selectivity Coefficient
hydrogen
H+
1.0
sodium
Na+
2.0
iron
Fe2+
2.9
zinc
Zn2+
3.0
cadmium
Cd2+
3.95
calcium
Ca2+
5.8
strontium
Sr2+
8.1
copper
Cu2+
14.5
mercury
Hg2+
14.0
lead
Pb2+
14.5
For example, if Hg is the cation that may interfere, then the associated adsorption material 34′ of the adsorption layer 34.1 could be doped, or saturated, with, for example, sodium, Na. If Hg breaks through the cation filter 188, 188′, then the Hg will displace the Na on the adsorption layer 34.1 of the associated first electrode 26 of the active piezoelectric resonator 20.1, and the resultant frequency response, i.e. lowered resonant frequency, will indicate the breakthrough.
If, in step (2418), a break-through has been detected by the second chemical monitor 190, then in step (2420), referring also to
A relatively high concentration of NaCl will displace Hg or As(III) despite the fact that Na is far below Hg on the selectivity sequence. If Hg is >8× more preferred than Na, they will reach chemical equilibrium when the concentration of Na is 8× that of Hg.
Otherwise, from step (2404), if neither anions or cations are to be filtered, then, in step (2422), the mechanically filtered sample fluid 14 is discharged from the preconditioner 2500 as the associated preconditioned sample fluid 14′.
The preconditioner 2500 may also include a granular activated carbon column—for example, in silver form to inhibit growth of bacteria—to remove organic molecules, upstream of the anion 176, 176′ or cation filters.
In one set of embodiments, the fluid contaminant sensing system 10 is configured to operate in a default pH range of 6.5-8.5, which is consistent with US tap water. Configuration in this sense means that the receptors will are designed to capture the targets in that range, and the separations will perform in that range. In monitor applications such as some industrial applications with pH's of the sample flow outside the default range, then the receptors and separations are designed accordingly.
In the event that the pH of the sample flow changes and moves out of the targeted range, then the associated pH control system adjust the pH of the sample flow and restore it to the target range.
As shown in
A continuous flow pH measurement device is employed upstream from the monitor. For pH measurement, from Cole Parmer, a Honeywell Directline pH Transmitter Electronics Module, EW56611-60 using a pH electrode EW-56611-62 can be employed.
In one embodiment, pH adjustment columns are employed to either raise or lower pH. The columns are connected to tubing that feeds into the sensor sample flow line. In one embodiment, the adjustment columns are a cation exchanger in the H+ form and an anion exchanger in the OH− form.
The pH measurements readings are recorded in the CCM. When the pH readings are outside the sensor range, the CCM will trigger microvalves preceding the upstream cation or anion columns as described below.
When the pH measurement device indicates the pH is higher than desired for a particular sensor, a solution of NaCl is passed through the cation column, releasing H+ ions into the sample flow, lowering the pH. The cation column is regenerated by passing HCl through the column and directing the effluent to a disposal container.
When the upstream pH measurement device indicates the pH is lower than desired for a particular sensor, a solution of NaCl is passed through the anion column, releasing OH− ions into the sample flow, increasing the pH. The anion exchanger is regenerated by passing NaOH through the column and directing the effluent to a disposal container.
Accordingly, depending upon the configuration, and the particular type of adsorption material 34′ used on the third electrode 30 of the associated reference piezoelectric resonator 20.2, the fluid contaminant sensing system 10 can provide for sensing target ions, molecules, inorganic or organic chemicals or microorganisms, in environments ranging from ultra-pure water systems to befouled waters. The fluid contaminant sensing system 10 provides for substantially unattended continuous flow sensing, or for use as a portable sampler and sensor, The results of the fluid contaminant sensing system 10 can be displayed locally, or transmitted to a location that is remote in relation to the associated sample fluid 14 and the fluid contaminant sensor cell 54 and associated physical hardware.
Notwithstanding that the above-illustrated embodiments incorporate quartz-crystal piezoelectric resonators 20, it should be understood that other types of piezoelectric material may also be used, including, but not limited to lithium tantalate, lithium niobate, lithium borate, berlinite, gallium arsenide, lithium tetraborate, aluminium phosphate, bismuth germanium oxide, polycrystalline zirconium titanate ceramics, high-alumina ceramics, silicon-zinc oxide composite, dipotassium tartrate, or PZT (lead zirconate titanate). An oscillator crystal can be also manufactured by depositing the resonator material on the silicon chip surface. Crystals of gallium phosphate, langasite, langanite and langanate are about 10 times more pullable than the corresponding quartz crystals, and are used in some VCXO (voltage controlled crystal oscillators) oscillators. Other VCXO materials that could be used include AIPO4, GaPO4, langasite La3Ga5NbO14 Lanthanum gallium niobate and langanite and langatate. In addition to its piezoelectric effect and the ability to operate as a controlled frequency resonator based upon its physical dimensions, the piezoelectric resonators 20 also provide sufficient strength to contain the fluid within the associated first 38 or second 40 cavity of the fluid contaminant sensor cell 54.
Notwithstanding that the third electrode 30 of the reference piezoelectric resonator 20.2 in the above-illustrated embodiments incorporate an adsorption layer 34.2 of adsorption material 34′ for which the associated chemical receptors are blocked with a blocking substance 36′ in an overlaying blocking layer 36—provide for the third electrode 30 of the reference piezoelectric resonator 20.2 to be configured as close as possible to the first electrode 26 of the active piezoelectric resonator 20.1, which provides for minimizing the effects of ionic strength, viscosity and other common-mode effects, —it should be understood that, depending upon the nature of the sample fluid 14 and the nature of the target analyte 12, that a blocked adsorption layer 34.2 is not essential, and that in some cases, the third electrode 30 of the reference piezoelectric resonator 20.2 could be either uncoated, or coated with a different substance, for example, TEFLON® or a TEFLON®-like substance.
While specific embodiments have been described in detail in the foregoing detailed description and illustrated in the accompanying drawings, those with ordinary skill in the art will appreciate that various modifications and alternatives to those details could be developed in light of the overall teachings of the disclosure. It should be understood, that any reference herein to the term “or” is intended to mean an “inclusive or” or what is also known as a “logical OR”, wherein when used as a logic statement, the expression “A or B” is true if either A or B is true, or if both A and B are true, and when used as a list of elements, the expression “A, B or C” is intended to include all combinations of the elements recited in the expression, for example, any of the elements selected from the group consisting of A, B, C, (A, B), (A, C), (B, C), and (A, B, C); and so on if additional elements are listed. Furthermore, it should also be understood that the indefinite articles “a” or “an”, and the corresponding associated definite articles “the’ or “said”, are each intended to mean one or more unless otherwise stated, implied, or physically impossible. Yet further, it should be understood that the expressions “at least one of A and B, etc.”, “at least one of A or B, etc.”, “selected from A and B, etc.” and “selected from A or B, etc.” are each intended to mean either any recited element individually or any combination of two or more elements, for example, any of the elements from the group consisting of “A”, “B”, and “A AND B together”, etc. Yet further, it should be understood that the expressions “one of A and B, etc.” and “one of A or B, etc.” are each intended to mean any of the recited elements individually alone, for example, either A alone or B alone, etc., but not A AND B together. Furthermore, it should also be understood that unless indicated otherwise or unless physically impossible, that the above-described embodiments and aspects can be used in combination with one another and are not mutually exclusive. Accordingly, the particular arrangements disclosed are meant to be illustrative only and not limiting as to the scope of the invention, which is to be given the full breadth of the appended claims, and any and all equivalents thereof.
Merrill, John H., Waldrop, III, Alex A., Becks, Eric R.
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