Tricyclic compounds of the general formula I: ##STR1## as defined herein which exhibit antagonist activity at V1 and/or V2 receptors and exhibit in vivo vasopressin antagonist activity, methods for using such compounds in treating diseases characterized by excess renal reabsorption of water, and processes for preparing such compounds.
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3. A compound of the formula: ##STR232## wherein; Y is a bond;
A--B is ##STR233## R1 is H, halogen (Cl, F, Br, I), OH, --S-lower alkyl (C1 -C3), --SH, --SO lower alkyl (C1 -C3), --SO2 lower alkyl (C1 -C3), --CO lower alkyl (C1 -C3), --CF3, lower alkyl (C1 -C3), --O lower alkyl (C1 -C3), --NO2, --NH2, --NHCO lower alkyl (C1 -C3), --N-[lower alkyl (C1 -C3)]2, SO2 NH2, --SO2 NH lower alkyl (C1 -C3), or --SO2 N [lower alkyl (C1 -C3)]2 ; R2 is selected from H, Cl, Br, I, F, --OH, lower alkyl (C1 -C3), or --O lower alkyl (C1 -C3); or R1 and R2 taken together are methylenedioxy or ethylenedioxy; R3 is the moiety ##STR234## wherein Ar is a moiety selected from the group ##STR235## R5 is H, --CH3, --C2 H5, Cl, Br, F, --O-CH3, or --O-C2 H5 ; R6 is selected from: ##STR236## wherein cycloalkyl is defined as C3 -C6 cycloalkyl, cyclohexenyl or cyclopentenyl; n is 0-2; and wherein Ar' is selected from the moieties: ##STR237## wherein Ra and Rb are independently selected from H, --CH3, or --C2 H5 ; R7 is H, --CH3, --C2 H5, Cl, Br, F, --O--CH3, --O--C2 H5 or --CF3 ; R8 and R9 are independently selected from hydrogen, lower alkyl (C1 -C3), O-lower alkyl (C1 -C3), S-lower alkyl (C1 -C3), --CF3, --CN, --OH, --SCF3, --OCF3, halogen, NO2, amino, or --NH-lower alkyl (C1 -C3); W' is selected from O, S, NH, N-lower alkyl (C1 -C3), --NCO-lower alkyl (C1 -C3), or NSO2 -lower alkyl (C1 -C3); the moiety ##STR238## represents a fused pyrazole ring or fused substituted pyrazole [4,3-d] ring optionally substituted by one or two substituents selected from (C1 -C3) lower alkyl, halogen, formyl, (C1 -C3) lower alkoxy, --CO2 H, (C1 -C3) lower alkoxycarbonyl, ##STR239## or a moiety of the formula: ##STR240## or a pharmaceutically acceptable salt or ester thereof. 4. A compound selected from those of the formula: ##STR241## wherein R1 is hydrogen, chlorine, bromine, fluorine, iodine, OH, S-lower alkyl (C1 -C3), --SH, --SO-lower alkyl(C1 -C3), --SO2 -lower alkyl(C1 -C3), --CO-lower alkyl(C1 -C3), --CF3, lower alkyl(C1 -C3), O-lower alkyl(C1 -C3), --NO2, --NH2, --NHCO lower alkyl(C1 -C3), --N-[lower alkyl (C1 -C3)]2, --SO2 NH2 ; --SO2 NH lower alkyl(C1 -C3), or --SO2 N[lower alkyl(C1 -C3)]2 ; R2 is hydrogen, Cl, Br, F, I, --OH, lower alkyl(C1 -C3), O-lower alkyl(C1 -C3), or R1 and R2 taken together are methylenedioxy or ethylenedioxy;
R3 is the moiety: ##STR242## wherein Ar is selected from moieties of the formula: ##STR243## and X is O, S, --NCH3 or --N-COCH3 ; R is independently selected from hydrogen, lower alkyl (C1 -C3), ##STR244## (C1 -C3)lower alkoxycarbonyl, --CO2 H, ##STR245## --(CH2)q --OH, --(CH2)q --O-alkyl(C1 -C3); q is one or two; R4 is selected from hydrogen, lower alkyl(C1 -C3), --CO-lower alkyl(C1 -C3); SO2 lower alkyl(C1 -C3); or moieties of the formulae: ##STR246## R5 is hydrogen, --CH3, --C2 H5, Cl, Br, F, --O--CH3 or --O--C2 H5 ; R6 is selected from (a) moieties of the formula: ##STR247## wherein cycloalkyl is defined as C3 -C6 cycloalkyl, cyclohexenyl or cyclopentenyl; Ra is hydrogen, CH3, C2 H5, moieties of the formuale: ##STR248## --(CH2)2 --O-lower alkyl(C1 -C3) or --CH2 CH2 OH; q is one or two; Rb is hydrogen, --CH3 or --C2 H5 ; or R6 is (b) a moiety of the formula: ##STR249## where R2 is as hereinbefore defined; or R6 is (c) a moiety of the formula: ##STR250## wherein J is Ra, lower alkyl (C1 -C8) branched or unbranched, lower alkenyl (C2 -C8) branched or unbranched, --O-lower alkyl (C1 -C8) branched or unbranched, --O-lower alkenyl (C2 -C8) branched or unbranched, tetrahydrofuran, tetrahydrothiophene, or --CH2 --K wherein K is halogen, --OH, tetrahydrofuran, tetrahydrothiophene or the heterocyclic ring moiety: ##STR251## wherein D, E, F and G are selected from carbon or nitrogen and wherein the carbon atoms may be optionally substituted with halogen, (C1 -C3)lower alkyl, hydroxy, --CO-lower alkyl (C1 -C3), CHO, (C1 -C3)lower alkoxy, or --CO2 -lower alkyl (C1 -C3), and Ra and Rb are as hereinbefore defined; (d) a moiety selected from those of the formulae: ##STR252## wherein Rc is selected from halogen, (C1 -C3) lower alkyl, --O-lower alkyl (C1 -C3) or OH; Rb is as hereinbefore defined; Ar' is selected from the group: ##STR253## R7 is hydrogen, --CH3, --CH2 H5, Cl, Br, F, --OCH3, --OC2 H5, or --CF3 ; R8 and R9 are independently hydrogen, lower alkyl (C1 -C3), O-lower alkyl (C1 -C3), S-lower alkyl (C1 -C3), --CF3, --CN, --OH, --SCF3, --OCF3, halogen, NO2, amino, or --NH-lower alkyl (C1 -C3); R10 is selected from the group of halogen, hydrogen, or lower alkyl (C1 -C3); W' is selected from O, S, NH, N-lower alkyl (C1 -C3), --NCO-lower alkyl (C1 -C3), or NSO2 -lower alkyl (C1 -C3); the moiety ##STR254## represents a fused pyrazole ring or fused substituted pyrazole ring optionally substituted by one or two substituents selected from (C1 -C3) lower alkyl, halogen, formyl, (C1 -C3) lower alkoxy, (C1 -C3) lower alkoxycarbonyl, --CO2 H, ##STR255## or a moiety of the formula: ##STR256## or a pharmaceutically acceptable salt, or ester thereof. 1. A compound selected from formula I: ##STR213## wherein; Y is a bond;
A--B is ##STR214## R1 is H, chlorine, fluorine, bromine, iodine, OH, --S-lower alkyl (C1 -C3), --SH, --SO lower alkyl (C1 -C3), --SO2 lower alkyl (C1 -C3), --CO lower alkyl (C1 -C3), --CF3, lower alkyl (C1 -C3), --O lower alkyl (C1 -C3), --NO2, --NH2, --NHCO lower alkyl (C1 -C3), --N--[lower alkyl (C1 -C3)]2, SO2 NH2, --SO2 NH lower alkyl (C1 -C3), or --SO2 N [lower alkyl (C1 -C3)]2 ; R2 is H, Cl, Br, I, F, -OH, lower alkyl (C1 -C3), --O lower alkyl (C1 -C3); or R1 and R2 taken together are methylenedioxy or ethylenedioxy; R3 is the moiety ##STR215## wherein Ar is a moiety selected from the group ##STR216## and X is selected from O, S, --NCH3, or --N--COCH3 ; R4 is selected from H, lower alkyl (C1 -C3), --CO-lower alkyl (C1 -C3), SO2 lower alkyl (C1 -C3), and the moieties of the formulae: ##STR217## R5 is H, --CH3, --C2 H5, Cl, Br, F, --O--CH3, or --O--C2 H5 ; R6 is selected from: (a) moieties of the formula: ##STR218## wherein cycloalkyl is defined as C3 -C6 cycloalkyl, cyclohexenyl or cyclopentenyl; R2 is as hereinbefore defined; n is 0-2; R7 is H, --CH3, --CH2 H5, Cl, Br, F, --OCH3, --OC2 H5, or --CF3 ; Ra is hydrogen, CH3, C2 H5, moieties of the formulae: ##STR219## --(CH2)2 --O-lower alkyl (C1 -C3) or --CH2 CH2 OH; q is one or two; Rb is hydrogen, --CH3 or --C2 H5 ; Ar' is selected from the group: ##STR220## wherein R4, R5 are as hereinbefore defined; R8 and R9 are independently hydrogen, lower alkyl (C1 -C3), O-lower alkyl (C1 -C3), S-lower alkyl (C1 -C3), --CF3, --CN, --OH, --SCF3, --OCF3, halogen, NO2, amino, or --NH-lower alkyl (C1 -C3); R10 is selected from halogen, hydrogen, or lower alkyl (C1 -C3); W' is selected from O, S, NH, N-lower alkyl (C1 -C3), --NCO-lower alkyl (C1 -C3), or NSO2 -lower alkyl (C1 -C3); or R6 is: (b) a moiety of the formula: ##STR221## where R2 is as hereinbefore defined or R6 is; (c) a moiety of the formula: ##STR222## wherein J is Ra, lower alkyl (C1 -C8) branched or unbranched, lower alkenyl (C2 -C8) branched or unbranched, --O-lower alkyl (C1 -C8) branched or unbranched, --O-lower alkenyl (C2 -C8) branched or unbranched, tetrahydrofuran, tetrahydrothiophene, or --CH2 --K wherein K is halogen, --OH, tetrahydrofuran, tetrahydrothiophene or the heterocyclic ring moiety: ##STR223## wherein D, E, F and G are selected from carbon or nitrogen and wherein the carbon atoms may be optionally substituted with halogen, (C1 -C3) lower alkyl, hydroxy, --CO-lower alkyl (C1 -C3), CHO, (C1 -C3) lower alkoxy, or --CO2 -lower alkyl (C1 -C3), and Ra and Rb are as hereinbefore defined or R6 is; (d) a moiety selected from those of the formulae: ##STR224## wherein Rc is selected from halogen, (C1 -C3) lower alkyl, --O-lower alkyl (C1 -C3) or OH; Rb is as hereinbefore defined; R7 is hydrogen, --CH3, --C2 H5, Cl, Br, F, --OCH3, --OC2 H5, or --CF3 ; R8 and R9 are independently hydrogen, lower alkyl (C1 -C3), O-lower alkyl (C1 -C3), S-lower alkyl (C1 -C3), --CF3, --CN, --OH, --SCF3, --OCF3, halogen, NO2, amino, or --NH-lower alkyl (C1 -C3); R10 is selected from the group of halogen, hydrogen, or lower alkyl (C1 -C3); W' is selected from O, S, NH, N-lower alkyl (C1 -C3), --NCO-lower alkyl (C1 -C3), or NSO2 -lower alkyl (C1 -C3); the moiety ##STR225## represents a fused pyrazole [4,3-d] ring or fused substituted pyrazole ring optionally substituted by one or two substituents selected from (C1 -C3) lower alkyl, halogen, formyl, (C1 -C3) lower alkoxy, (C1 -C3) lower alkoxycarbonyl, --CO2 H, ##STR226## or a moiety of the formula: ##STR227## or a pharmaceutically acceptable salt or ester thereof. 2. A compound according to
R3 is the moiety ##STR228## wherein Ar is a moiety selected from the group ##STR229## R6 is selected from the group ##STR230## or --CH2 COAr'; wherein n is 0-2; Ar' is ##STR231## W' is O or S; A--B, Ra, Rb, R1, R2, R4, R5, R7, R8, R9, and cycloalkyl are as defined in claim 1; or a pharmaceutically acceptable salt or ester thereof.
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55. A pharmaceutical composition useful for treating disease in a mammal characterized by excess renal reabsorption of water, the pharmaceutical composition comprising an effective amount of a compound of
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57. A method for treating disease in a mammal characterized by excess renal reabsorption of water, the method comprising administering to a mammal in need thereof an effective amount of a compound of
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This application is a continuation of application Ser. No. 08/663,400, filed Jun. 13, 1996, now abandoned, which is a continuation-in-part application Ser. No. 08/639,014, filed Apr. 24, 1996, which is a continuation-in-part of application Ser. No. 08/254,823, filed Jun. 13, 1994, now U.S. Pat. No. 5,512,563, which is a continuation-in-part of application Ser. No. 08/100,003, filed Jul. 29, 1993, now abandoned.
This invention relates to new tricyclic non-peptide vasopressin antagonists which are useful in treating conditions where decreased vasopressin levels are desired, such as in congestive heart failure, in disease conditions with excess renal water reabsorption and in conditions with increased vascular resistance and coronary vasoconstriction.
Vasopressin is released from the posterior pituitary either in response to increased plasma osmolarity detected by brain osmoreceptors or decreased blood volume and blood pressure sensed by low-pressure volume receptors and arterial baroreceptors. The hormone exerts its action through two well defined receptor subtypes: vascular V1 and renal epithelial V2 receptors. Vasopressin-induced antidiuresis, mediated by renal epithelial V2 receptors, helps to maintain normal plasma osmolarity, blood volume and blood pressure.
Vasopressin is involved in some cases of congestive heart failure where peripheral resistance is increased. V1 antagonists may decrease systemic vascular resistance, increase cardiac output and prevent vasopressin induced coronary vasoconstriction. Thus, in conditions with vasopressin induce increases in total peripheral resistance and altered local blood flow, V1 -antagonists may be therapeutic agents. V1 antagonists may decrease blood pressure, induce hypotensive effects and thus be therapeutically useful in treatment of some types of hypertension.
The blockade of V2 receptors is useful in treating diseases characterized by excess renal reabsorption of free water. Antidiuresis is regulated by the hypothalamic release of vasopressin (antidiuretic hormone) which binds to specific receptors on renal collecting tubule cells. This binding stimulates adenylyl cyclase and promotes the cAMP-mediated incorporation of water pores into the luminal surface of these cells. V2 antagonists may correct the fluid retention in congestive heart failure, liver cirrhosis, nephrotic syndrome, central nervous system injuries, lung disease and hyponatremia.
Elevated vasopressin levels occur in congestive heart failure which is more common in older patients with chronic heart failure. In patients with hyponatremic congestive heart failure and elevated vasopressin levels, a V2 antagonist may be beneficial in promoting free water excretion by antagonizing the action of antiduretic hormone. On the basis of biochemical and pharmacological effects of the hormone, antagonists of vasopressin are expected to be therapeutically useful in the treatment and/or prevention of hypertension, cardiac insufficiency, coronary vasospasm, cardiac ischemia, renal vasospasm, liver cirrhosis, congestive heart failure, nephrotic syndrome, brain edema, cerebral ischemia, cerebral hemorrhage-stroke, thrombosis-bleeding and abnormal states of water rentention.
The following prior art references describe peptide vasopressin antagonists: M. Manning et al., J. Med. Chem., 35, 382(1992); M. Manning et al., J. Med. Chem., 35, 3895(1992); H. Gavras and B. Lammek, U.S. Pat. No. 5,070,187 (1991); M. Manning and W. H. Sawyer, U.S. Pat. No. 5,055,448(1991); F. E. Ali, U.S. Pat. No. 4,766,108(1988); R. R. Ruffolo et al., Drug News and Perspective, 4(4), 217, (May)(1991). P. D. Williams et al., have reported on potent hexapeptide oxytocin antagonists [J. Med. Chem., 35, 3905(1992)] which also exhibit weak vasopressin antagonist activity in binding to V1 and V2 receptors. Peptide vasopressin antagonists suffer from a lack of oral activity and many of these peptides are not selective antagonists since they also exhibit partial agonist activity.
Non-peptide vasopressin antagonists have recently been disclosed, Y. Yamamura et al., Science, 252, 579(1991); Y. Yamamura et al., Br. J. Pharmacol, 105, 787(1992); Ogawa et al., (Otsuka Pharm Co., LTD.) EP 0514667-A1; JP 04154765-A; EPO 382185-A2; and WO09105549. Ogawa et al, (Otsuka Pharm. Co.) EP 470514A disclose carbostyril derivatives and pharmaceutical compositions containing the same. Non-peptide oxytocin and vasopressin antagonist have been disclosed by Merck and Co.; M. G. Bock and P. D. Williams, EP 0533242A; M. G. Bock et al., EP 0533244A; J. M. Erb, D. F. Verber, P. D. Williams, EP 0533240A; K. Gilbert et al., EP 0533243A.
Premature birth can cause infant health problems and mortality and a key mediator in the mechanism of labor is the peptide hormone oxytocin. On the basis of the pharmacological action of oxytocin, antagonists of this hormone are useful in the prevention of preterm labor, B. E. Evans et al., J. Med. Chem. 35, 3919 (1992), J. Med. Chem., 36, 3993 (1993) and references therein. The compounds of this invention are antagonists of the peptide hormone oxytocin and are useful in the control of premature birth.
The present invention relates to novel tricyclic derivatives which exhibit antagonist activity at V1 and/or V2 receptors and exhibit in vivo vasopressin antagonist activity. The compounds also exhibit antagonist activity at oxytocin receptors.
This invention relates to new compounds selected from those of the general formula I: ##STR2## wherein Y is (CH2)n, O, S, NH, NCOCH3, N-lower alkyl (C1 -C3), CH-lower alkyl(C1 -C3), CHNH-lower alkyl
(C1 -C3), CHNH2, CHN[lower alkyl (C1 -C3)]2, CHO-lower alkyl(C1 -C3), CHS-lower alkyl(C1 -C3), or the moiety: ##STR3## wherein n is an integer from 0-2; A--B is ##STR4## wherein m is an integer from 1-2, provided that when Y is --(CH2)n -- and n=2, m may also be zero and when n is zero, m may also be three, provided also that when Y is --(CH2)n -- and n is 2, m may not also be two.
R1 is hydrogen, halogen (chlorine, bromine, fluorine, iodine), OH, --S-lower alkyl(C1 -C3), --SH, --SO lower alkyl(C1 -C3), --SO2 -lower alkyl(C1 -C3), --CO-lower alkyl(C1 -C3), --CF3 ; lower alkyl(C1 -C3); O-lower alkyl(C1 -C3), --NO2, --NH2, --NHCO lower alkyl(C1 -C3), --N--[lower alkyl(C1 -C3)]2, --SO2 NH2 ; --SO2 NH lower alkyl (C1 -C3) or --SO2 N[lower alkyl (C1 -C3)]2 ;
R2 is hydrogen, Cl, Br, F, I, --OH, lower alkyl(C1 -C3), O-lower alkyl(C1 -C3), or R1 and R2 taken together are methylenedioxy or ethylenedioxy;
R3 is the moiety: ##STR5## wherein Ar is a moiety selected from the group ##STR6## and X is O, S, --NCH3, or --N--COCH3 ; R4 is hydrogen, lower alkyl(C1 -C3); --CO-lower alkyl (C1 -C3); phenylCO, phenylSO2 ; tolylSO2 ; SO2 lower alkyl (C1 -C3); or moieties of the formulae: ##STR7## R5 is hydrogen, --CH3, --C2 H5, Cl, Br, F, --O--CH3, or --O--C2 H5 ;
R6 is selected from (a) moieties of the formula: ##STR8## alkyl(C3 -C8) straight or branched, ##STR9## alkyl(C3 -C8) straight or branched, --NHSO2 -lower alkyl(C3 -C8) straight or branched, ##STR10## alkanyl(C3 -C8) straight or branched, ##STR11## C-lower alkenyl(C3 -C8) straight or branched, --NHSO2 -lower alkenyl(C3 -C8) straight or branched,
wherein cycloalkyl is defined as C3 to C6 cycloalkyl, cyclohexenyl or cyclopentenyl; Ra is hydrogen, CH3, C2 H5, moieties of the formulae: ##STR12## --(CH2)2 O-lower alkyl (C1 -C3) or --CH2 CH2 OH; q is one or two; Rb is hydrogen, CH3 or --C2 H5 ;
(b) a moiety of the formula: ##STR13## where R2 is as hereinbefore defined; (c) a moiety of the formula: ##STR14## wherein J is Ra, lower alkyl(C1 -C8) branched or unbranched, lower alkenyl(C2 -C8) branched or unbranched, O-lower alkyl(C1 -C8) branched or unbranched, --O-lower alkenyl(C2 -C8) branched or unbranched, tetrahydrofuran, tetrahydrothiophene, or --CH2 --K wherein K is halogen, --OH, tetrahydrofuran, tetrahydrothiophene or the heterocyclic ring moiety: ##STR15## wherein D, E, F and G are selected from carbon or nitrogen and wherein the carbon atoms may be optionally substituted with halogen, (C1 -C3)lower alkyl, hydroxy, --CO-lower alkyl(C1 -C3), CHO, (C1 -C3)lower alkoxy, --CO2 -lower alkyl(C1 -C3), and Ra and Rb are as hereinbefore defined;
(d) a moiety selected from those of the formulae: ##STR16## wherein Rc is selected from halogen, (C1 -C3)lower alkyl, --O-lower alkyl(C1 -C3) and OH, Rb is as hereinbefore defined;
Ar' is a moiety selected from the group ##STR17## R7 is hydrogen, --CH3, --C2 H5, Cl, Br, F, --OCH3, --OC2 H5, or --CF3 ;
R8 and R9 are independently hydrogen, lower alkyl (C1 -C3); O-lower alkyl(C1 -C3); S-lower alkyl (C1 -C3), --CF3, --CN, --OH, --SCF3, --OCF3, halogen, NO2, amino or NH lower alkyl(C1 -C3);
R10 is halogen, hydrogen or lower alkyl(C1 -C3); W' is O, S, NH, N-lower alkyl(C1 -C3), NCO-lower alkyl(C1 -C3) or NSO2 -lower alkyl(C1 -C3). ##STR18## represents: (1) fused phenyl or fused substituted phenyl optionally substituted by one or two substituents selected from (C1 -C3) lower alkyl, halogen, amino, (C1 C3) lower alkoxy, or (C1 -C3) lower alkylamino; (2) a 5-membered aromatic (unsaturated) heterocyclic ring having one heteroatom selected from O, N or S; (3) a 6-membered aromatic (unsaturated) heterocyclic ring having one nitrogen atom; (4) a 5 or 6-membered aromatic (unsaturated) heterocyclic ring having two nitrogen atoms; (5) a 5-membered aromatic (unsaturated) heterocyclic ring having one nitrogen atom together with either one oxygen or one sulfur atom; wherein the 5 or 6-membered heterocyclic rings are optionally substituted by (C1 -C3) lower alkyl, formyl, (C1 -C3) lower alkoxycarbonyl, CO2 H, ##STR19## a moiety of the formula: ##STR20## halogen or (C1 -C3) lower alkoxy. For example, the fused heterocyclic ring may be represented by furan, pyrrole, pyrazole, thiophene, thiazole, oxazole, imidazole, pyrimidine or pyridine ring which may be substituted or unsubstituted.
Within the group of the compounds defined by Formula I, certain subgroups of compounds are broadly preferred. Broadly preferred are those compounds wherein R3 is a moiety: ##STR21## and Ar is selected from the moiety: ##STR22## wherein R5, R6 and R7 are as hereinbefore defined.
Especially preferred are compounds wherein R3 is the moiety: ##STR23## and Ar is selected from the moiety: ##STR24## R6 is NHCOAr' and Ar' is ##STR25## wherein R8, R9 and W' are as hereinbefore defined.
Also especially broadly preferred are compounds wherein Y in Formula I is --(CH2)n -- and n is zero or one; A--B is ##STR26## and R4, R5, R6, R7, R8, R9 and R10 are as hereinbefore defined; and m is an integer from 1-2.
The most broadly preferred of the compounds of Formula I are those wherein Y is --(CH2)n -- and n is one; A--B is ##STR27## R3 is the moiety: ##STR28## Ar is ##STR29## R6 is ##STR30## and Ar' is a moiety: ##STR31## Cycloalkyl and W' are as previously defined and R8 and R9 are preferably ortho CF3, Cl, OCH3, CH3, SCH3 or OCF3 substituents or Ar' is a disubstituted derivative wherein R8 and R9 are independently Cl, OCH3, CH3.
The most highly broadly preferred of the compounds of Formula I are those wherein Y is --(CH2)n --, n is zero or one and ##STR32## represents a fused phenyl, substituted phenyl, thiophene, furan, pyrrole or pyridine ring;
A--B is ##STR33## m is one when n is one and m is two when n is zero; R3 is the moiety: ##STR34## wherein Ar is ##STR35## and R6 is selected from the group ##STR36## where Ar' is selected from the group ##STR37## and Ra, Rb, R1, R2, R4, R5, R6, R7, R8, R9 and W' are as previously defined.
Most particularly preferred are compounds of the formulae: ##STR38## wherein m is an integer one or two; R1 and R2 are as previously defined;
R3 is the moiety: ##STR39## wherein Ar is selected from moieties of the formulae: ##STR40## R6 is ##STR41## wherein cycloalkyl is defined as C3 -C6 cycloalkyl, cyclohexenyl or cyclopentenyl and wherein Ar' is selected from the moieties: ##STR42## Ra is independently selected from hydrogen, CH3 or --C2 H5 ; and R5, R7, R8, R9, R10 and W' are as hereinbefore defined.
Compounds of this invention may be prepared as shown in Scheme I by reaction of tricyclic derivatives of Formula 3a and 3b with a substituted or unsubstituted 4-nitrobenzoyl chloride 4 to give the intermediates 5a and 5b. Reduction of the nitro group in intermediates 5a and 5b gives the 4-aminobenzoyl derivatives 6a and 6b. The reduction of the nitro group in intermediates 5a and 5b may be carried out under catalytic reduction conditions (hydrogen-Pd/C; Pd/C- hydrazine-ethanol) or under chemical reduction conditions (SnCl2 -ethanol; Zn-acetic acid; TiCl3) and related reduction conditions known in the art for converting a nitro group to an amino group. The conditions for conversion of the nitro group to the amino group are chosen on the basis of compatibility with the preservation of other functional groups in the molecule.
Reaction of compounds of Formula 6a and 6b with aroyl chloride or related activated aryl carboxylic acids in solvents such as chloroform, dichloromethane, dioxane, tetrahydrofuran, toluene and the like in the presence of a tertiary base such as triethylamine and diisopropylethylamine or pyridine and the like, affords the compounds 8a and 8b which are vasopressin antagonists. ##STR43##
Reaction of tricyclic derivatives of Formula 6a and 6b with either a carbamoyl derivative 9 or a isocyanate derivative 10 gives compounds (Scheme 2) of Formula 11a and 11b which are vasopressin antagonists of Formula I wherein R6 is ##STR44## and Rb is H, CH3 or C2 H5. ##STR45##
Reaction of tricyclic derivatives of Formula 6a and 6b with arylacetic acids, activated as the acid chlorides 12, anhydrides, mixed anhydrides or activated with known activating reagents, gives compounds 13a and 13b (Scheme 3). ##STR46##
The compounds of Formula I wherein Y, A--B, Z, R1, R2 and R3 are as defined and the aryl of R3 (--COAr) is ##STR47## may be prepared, as shown in Scheme 4, by reacting an activated ester of the indole-5-carboxylic acids 14 with tricyclic derivatives 3a and 3b. The indole-5-carboxylic acids 14 may be activated by preparing the anhydride, a mixed anhydride or reacting with diethyl cyanophosphonate, N,N-carbonyldiimidazole or related peptide coupling reagents. As an example, the derivative 15 may be prepared by the reaction of acid 14 and N,N-carbonyldiimidazole in tetrahydrofuran; the solvent is removed and the derivative reacted with 3a or 3b at 100°C to 120°C without a solvent. Alternatively, 3a and 3b may be reacted with 15 in a solvent such as toluene or xylene at reflux temperatures. The activating reagent for the indole acids 14 is chosen on the basis of its compatibility with the R4 group and its reactivity with the tricyclic derivatives 3a and 3b to give the vasopressin antagonists 16a and 16b. ##STR48##
The compounds of Formula I wherein Y, A--B, Z, R1, R2 and R3 are as defined and the R3 (--COAr) aryl group is ##STR49## wherein R6 is ##STR50## may be prepared as shown in Scheme 5 by first reacting the derivatives 8a and 8b with sodium hydride or similar reagents to form the amide anion and then reacting the anion with a dialkoxyphosphoryl chloride to give the intermediates 17a and 17b. Reaction of these intermediates with sodium or lithium azide gives the products 18a and 18b. ##STR51##
Alternatively, the products 18a and 18b may be prepared by coupling tetrazole derivatives of the Formula 19 with tricyclic derivatives 3a and 3b (Scheme 6). The tetrazole carboxylic acids are activated for coupling to the tricyclic compounds 3a and 3b by reaction with peptide coupling reagents, by conversion to the acid chlorides, anhydrides or mixed anhydrides. ##STR52##
As an alternative method for synthesis of compounds of this invention as depicted in Formula I wherein Y, A--B, R1, R2 and Z are as previously defined and R3 is ##STR53## is the coupling of aryl carboxylic acids 20a with the tricyclic derivatives 3a and 3b as shown in Scheme 7.
The aryl carboxylic acids are activated for coupling by conversion to an acid chloride, bromide or anhydride or by first reacting with an activating reagent such as N,N-dicyclocarbodiimide, diethyl cyanophosphonate and related "peptide type" activating reagents. The method of activating the acids 20a for coupling to the tricyclic derivatives 3a and 3b is chosen on the basis of compatibility with other substituent groups in the molecule. The method of choice is the conversion of the aryl carboxylic acids 20a to the corresponding aroyl chloride. The aryl acid chlorides 20 may be prepared by standard procedures known in the art, such as reaction with thionyl chloride, oxalyl chloride and the like. The coupling reaction is carried out in solvents such as halogenated hydrocarbons, toluene, xylene, tetrahydrofuran dioxane in the presence of pyridine or tertiary bases such as triethylamine and the like. Alternatively, the aroyl chlorides, prepared from the aryl carboxylic acids 20, may be reacted with derivatives 3a and 3b in pyridine with or without 4-(dimethylamino)pyridine to give derivatives 21a and 21b.
In general, when the aryl carboxylic acids are activated with N,N-carbonyldiimidazole and other "peptide type" activating reagents, higher temperatures are required than when the aroyl chlorides are used. The reaction may be carried out in a higher boiling solvent xylene or without a solvent (100°C to 150°C). ##STR54##
The starting materials 3a and 3b in Scheme 1 can be made by literature methods. For example, intermediate 6,11-dihydro-5H-dibenz[b,e]azepines and substituted derivatives are prepared according to literature procedures: L. H. Werner, et al., J. Med. Chem., 8, 74-80 (1965); A. W. H. Wardrop et al., J. Chem. Soc. Perkins Trans I, 1279-1285 (1976).
Substituted 5,11-dihydrodibenz[b,e]azepin-6-one are prepared by literature procedures: J. Schmutz et al., Helv. Chim. Acta., 48, 336 (1965); and reduced to substituted 6,11-dihydro-5H-dibenz[b,e]azepines with lithium aluminum hydride, diborane, diborane-dimethyl-sulfide and agents known to reduce an amide carbonyl to a methylene group. Intermediate 10,11-dihydrodibenz[b, f][1,4]thiazepines are prepared by literature procedures--for example, see K. Brewster et al., J. Chem. Soc. Perkin I, 1286 (1976). Reduction of either dibenz[b,f][1,4]oxazepines [A. W. H. Wardrop et al., J. Chem. Soc. Perkin Trans. I, 1279 (1976)] and dibenz[b,f][1,4]oxazepin-11(10H)-ones and dibenz[b,f][1,4]thiazepin-11(10H)-ones--J. Schmutz et al., Helv. Chim. Acta, 48, 336 (1965); may be carried out with lithium aluminum hydride in inert solvents such as dioxane and the like. The tricyclic 6,7-dihydro-5H-dibenz[b,d]azepine intermediates of Formula 30 may be prepared by the literature procedures: T. Ohta et al., Tetrahedron Lett., 26, 5811 (1985); Wiesner et al., J. Amer. Chem. Soc., 77, 675 (1955); or derivatives may be prepared by coupling procedures illustrated in Scheme 8. The reduction of nitro compounds of structure type 31 followed by ring closure, affords lactams 32 which are reduced to give tricyclic azepines of Formula 33.
5,11-Dihydro-6H-pyrido[3,2-c][1]benzazepines are prepared by literature procedures--J. Firl et. al., Liebigs Ann. Chem. 469, (1989). Tricyclic 1,2,3,4-tetrahydropyrazolo[4,3-c][1]benzazepines are synthesized as described in the literature--G.
Palazpino et. al., J. Heterocyclic Chem., 26, 71 (1989). ##STR55##
Tricyclic intermediates 42 for the synthesis of selected vasopressin antagonists of this invention wherein Y in Formula I is --CH2 -- and m is one, may be prepared as shown in Scheme 9. Suitable 1-nitro-2-chloro or 1-nitro-2-bromo heterocycles 35 undergo halogen exchange when reacted with an alkyllithium reagent such as t-butyllithium, s-butyllithium or n-butyllithium to give intermediates 37 which react with anhydrides of Formula 38. R12 is tert-butyl, secondary butyl, n-butyl, 2,6-dimethylpiperidine or a hindered non-nucleophilic dialkylamine. The nitro products 39 are reduced with hydrogen and a suitable catalyst or chemically reduced (Zn-acetic acid, TiCl3 etc.) to the amino intermediates 40. Ring closure to the cyclic lactams 41 is conveniently carried out by heating in xylene or an inert solvent at 100°C to 200°C The cyclic lactams of structure type 41 are readily reduced by diborane in tetrahydrofuran, diborane-dimethylsulfide in tetrahydrofuran or lithium aluminum hydride in a suitable solvent such as dioxane to give the tricyclic compounds 42. ##STR56##
Alternatively, as shown in Scheme 10, some of the tricyclic derivatives of structural type 42 may be prepared by "palladium" type coupling or "copper" induced coupling of halogenated derivatives 43 to give tricyclic lactams 44. Reduction of the lactam carbonyl group gives the intermediates 42. Coupling of halogen derivatives 45 to effect ring closure with activated copper or "palladium" type reagents which induce aryl coupling gives lactams 46. Diborane reduction of lactams 46 gives derivatives 47. Ullmann cross-couplings of halogenated heterocycles and 2-bromo-nitrobenzenes and related cross-couplings by low valent palladium species such as [Pd(PPh3)4 ] and PdCl2 (PPh3)2 are known synthetic procedures; N. Shimizu et al., Tetrahedron Lett. 34, 3421 (1993) and references therein; N. M. Ali et al., Tetrahedron, 37, 8117 (1992) and references therein; J. Stavenuiter et al., Heterocycles, 26, 2711 (1987) and references therein. ##STR57##
Tetrahydro-1H-1-benzazepin-5-ones 51 and the tetrahydro-1H-1-benzazepin-2,5-diones 52 are useful compounds for the synthesis of intermediate tricyclic heterocyclic structures 53 and 54 (Scheme 11). The tetrahydrobenzazepin-5-ones 51 and 52 may be formylated to give hydroxymethylene derivatives or reacted with either the Vilsmeier reagent or the N,N-dimethylformamide dimethyl acetal to give the dimethylaminomethylene derivatives. The construction of heterocyclic rings from α-hydroxymethyleneketones by reactions with hydrazine N-methylhydrazine, hydroxylamine or formamidine to give pyrazoles, N-methylpyrazoles, oxazoles or pyrimidines respectively, is a standard literature procedure. See Vilsmeier formylation--Tetrahedron, 49, 4015-4034 (1993) and references therein and ring formations--J. Heterocyclic Chem., 29, 1214 (1992) and references therein.
Substituted and unsubstituted tetrahydrobenzazepin-2-ones are known compounds which are prepared by reaction of α-tetralones with sodium azide under acidic conditions. [J. Chem. Soc. 456 (1937); Tetrahedron 49, 1807 (1993)] (Schmidt reaction). Reduction of tetrahydro-1H-benzazepin-2-ones gives the tetrahydro-1H-benzazepines 48 which on acylation gives compounds 49. Oxidation of N-acyl tetrahydro-1H-benzapines of type 49 to give the 5-one derivatives is a known oxidative procedure; R. L. Augustine and W. G. Pierson, J. Org. Chem., 34, 1070 (1969).
The synthesis of 3,4-dihydro-1H-1-benzazepine-2,5-diones (52:R15 =H) has been reported as well as the conversion of 3,4-dihydro-1H-1-benzazepine-2,5-diones to 4-[(dimethylamino)methylene]-3,4-dihydro-1H-1-benzazepine-2,5-diones with N,N-dimethylformamide, dimethylacetal:[W. Y. Chen and N. W. Gilman, J. Heterocyclic Chem., 20, 663 (1983)]. The preceding reference describes the synthesis of 2-methyl-5,7-dihydropyrimido [5,4-d][1]benzazepin-6(6H)-ones which may be reduced to remove the lactam carbonyl group to give tricyclic derivatives of structural type 54 wherein Z is a pyrimidine ring. ##STR58##
The synthesis of compounds of Formula I wherein R3 is ##STR59## the Ar group is ##STR60## R6 is ##STR61## and where Ar' is as previously defined is carried out according to Scheme 12. The tricyclic compounds 3a and 3b are reacted with mono-methyl terephythalyl chloride 55 (prepared from mono-methyl terephthalate and thionyl chloride) in the presence of a tertiary base such as triethylamine in solvents such as dichloromethane, tetrahydrofuran, dioxane, toluene and the like to give derivatives 56a and 56b. These ester intermediates (56a and 56b) are hydrolyzed with two to ten equivalents of an alkaline hydroxide such as potassium or sodium hydroxide in aqueous methanol or ethanol to give the corresponding acids after acidification and workup. The free acids are converted to the acid chlorides with thionyl chloride and these acid chloride intermediates, 57a and 57b, reacted with aminoaryl derivatives of formula: ##STR62## wherein Ar' is as previously defined to give compounds 59a and 59b. ##STR63##
As described in reaction Scheme 1, the following specific tricyclic ring systems of the generic formula 3a and 3b are illustrated to show one of the synthetic methods for the synthesis of the compounds of this invention. These derivatives 60, 63, 66, 69, 72, 75, 78, 81, 84, 87, 90, and 93 when subjected to the reaction conditions in Scheme 1 which is the acylation of the tricyclic compounds (R16 ═H) with 4-nitrobenzoyl chloride or a substituted 4-nitrobenzoyl chloride in the presence of a trialkylamine such as triethylamine in solvents such as chloroform, dichloromethane, dioxane, tetrahydrofuran, toluene and the like give the intermediates 61, 64, 67, 70, 73, 76, 79, 82, 85, 88, 91, 94. These 4-nitrobenzoyl and substituted 4-nitro-benzoyl derivatives are reduced with hydrogen in the presence of a catalyst such as Pd/C in solvents such as ethanol, ethanol-ethyl acetate, acetic acid or N,N-dimethylformamide to give the 4-aminobenzyl or substituted 4-aminobenzoyl derivatives 62, 65, 68, 71, 74, 77, 80, 83, 86, 89, 92, 95. Alternatively, the 4-nitrobenzoyl and substituted 4-nitrobenzoyl derivatives 61, 64, 67, 70, 73, 76, 79, 82, 85, 88, 91, 94 are reduced with Pd/C and hydrazine in refluxing ethanol.
The 4-aminobenzoyl and substituted 4-aminobenzoyl derivatives 62, 65, 68, 71, 74, 77, 80, 83, 86, 89, 92, 95 are reacted with acid chlorides of the formula:
Ar' COCl, ##STR64## cycloalkyl(CH2) COCl, Ar'CH2 COCl, to give products as shown in Scheme 1 wherein R6 is as defined.
| ______________________________________ |
| ##STR65## |
| 60 (R16 = H) 5,6-dihydrophenanthridine |
| 61 |
| ##STR66## |
| 62 |
| ##STR67## |
| ##STR68## |
| 63 (R16 = H) 6,7-dihydro-5H-dibenz[b,d]azepine |
| 64 |
| ##STR69## |
| 65 |
| ##STR70## |
| ##STR71## |
| 66 (R16 = H) 6,11-dihydro-5H-dibenz[b,e]azepine |
| 67 |
| ##STR72## |
| 68 |
| ##STR73## |
| ##STR74## |
| 69 (R16 = H) 10,11-dihydrodibenz[b,f][1,4]oxazepine |
| 70 |
| ##STR75## |
| 71 |
| ##STR76## |
| ##STR77## |
| 72 (R16 = H) 10,11-dihydrodibenz[b,f][1,4]thiazepine |
| 73 |
| ##STR78## |
| 74 |
| ##STR79## |
| ##STR80## |
| 75 (R16 = H) 5,11-dihydro-10H-dibenz[b,e][1,4]diazepine |
| 76 |
| ##STR81## |
| 77 |
| ##STR82## |
| ##STR83## |
| 78 (R16 = H) 5,11-dihydro-6H-pyrido[3,2-c][1]benzazepine |
| 79 |
| ##STR84## |
| 80 |
| ##STR85## |
| ##STR86## |
| 81 (R16 = H) 5,6-dihydropyride[2,3-b][1,4]benzothlazepine |
| 82 |
| ##STR87## |
| 83 |
| ##STR88## |
| ##STR89## |
| 84 (R16 = H) 6,7-dihydro-5H-pyrimido[5,4-d][1]benzazepine |
| 85 |
| ##STR90## |
| 86 |
| ##STR91## |
| ##STR92## |
| 87 (R16 = H) 4,10-dihydro-5H-thieno[3,2-c][1]benzazepine |
| 88 |
| ##STR93## |
| 89 |
| ##STR94## |
| ##STR95## |
| 90 (R16 = H) 4,10-dihydro-9H-thiene[2,3-c][1]benzazepine |
| 91 |
| ##STR96## |
| 92 |
| ##STR97## |
| ##STR98## |
| 93 (R16 = H) 5,6,11,12-tetrahydrodibenz[b,f]azocine |
| 94 |
| ##STR99## |
| 95 |
| ##STR100## |
| ______________________________________ |
As described in Scheme 2, the tricyclic 4-aminobenzoyl or substituted 4-aminobenzoyl derivatives 62, 65, 68, 71, 74, 77, 80, 83, 86, 89, 92, and 95 are reacted with carbamoyl derivatives ##STR101## or arylisocyanates
Ar'--N═C═O
to give specific derivatives wherein R6 is ##STR102## and Rb is independently selected from H, CH3 or --C2 H5.
As described in Scheme 7, the tricyclic derivatives 60, 63, 66, 69, 72, 75, 78, 81, 84, 87, 90, and 93 are reacted in one step with a previously synthesized ArCOCl compound wherein Ar is as previously defined. For example, reaction of the specific tricyclic derivatives 60, 63, 66, 69, 72, 75, 78, 81, 84, 87, 92, 93 with aroyl chlorides of the following structural types: ##STR103## gives in one step derivatives of these delineated tri-cyclic compounds wherein the Ar group of the moiety: ##STR104## in formula I is ##STR105## and R6 is ##STR106## wherein cycloalkyl, Ra, Rb, R1, R2, R5, R7 and Ar' are as hereinbefore described.
Among the more preferred compounds of this invention are those selected from Formula I: ##STR107## wherein; Y is a bond;
A--B is ##STR108## R1 is H, halogen (chlorine, fluorine, bromine, iodine), OH, --S-lower alkyl (C1 -C3), --SH, --SO lower alkyl (C1 -C3), --SO2 lower alkyl (C1 -C3), --CO lower alkyl (C1 -C3), --CF3, lower alkyl (C1 -C3), --O lower alkyl (C1 -C3), --NO2, --NH2, --NHCO lower alkyl (C1 -C3), --N-[lower alkyl (C1 -C3)]2, SO2 NH2, --SO2 NH lower alkyl (C1 -C3), or --SO2 N [lower alkyl (C1 -C3)]2 ;
R2 is H, Cl, Br, I, F, --OH, lower alkyl (C1 -C3), --O lower alkyl (C1 -C3); or
R1 and R2 taken together are methylenedioxy or ethylenedioxy;
R3 is the moiety ##STR109## wherein Ar is a moiety selected from the group ##STR110## and X is selected from O, S, --NCH3, or --N--COCH3 ; R4 is selected from H, lower alkyl (C1 -C3), --CO-lower alkyl (C1 -C3), SO2 lower alkyl (C1 -C3), and the moieties of the formulae: ##STR111## R5 is H, --CH3, --C2 H5, Cl, Br, F, --O--CH3, or --O--C2 H5 ;
R6 is selected from:
(a) moieties of the formula: ##STR112## wherein cycloalkyl is defined as C3 -C6 cycloalkyl, cyclohexenyl or cyclopentenyl;
R2 is as hereinbefore defined;
n is 0-2;
R7 is H, --CH3, --CH2 H5, Cl, Br, F, --OCH3, --OC2 H5, or --CF3 ;
Ra is hydrogen, CH3, C2 H5, moieties of the formulae: ##STR113## --(CH2)2 --O-lower alkyl (C1 -C3) or --CH2 CH2 OH;
q is one or two;
Rb is hydrogen, --CH3 or --C2 H5 ;
Ar' is selected from the group: ##STR114## wherein R4, R5 are as hereinbefore defined;
R8 and R9 are independently hydrogen, lower alkyl (C1 -C3), O-lower alkyl (C1 -C3), S-lower alkyl (C1 -C3), --CF3, --CN, --OH, --SCF3, --OCF3, halogen, NO2, amino, or --NH-lower alkyl (C1 -C3);
R10 is selected from halogen, hydrogen, or lower alkyl (C1 -C3);
W' is selected from O, S, NH, N-lower alkyl (C1 -C3), --NCO-lower alkyl (C1 -C3), or NSO2 -lower alkyl (C1 -C3);
and
(b) a moiety of the formula: ##STR115## where R2 is as hereinbefore defined; (c) a moiety of the formula: ##STR116## wherein J is Ra, lower alkyl (C1 -C8) branched or unbranched, lower alkenyl (C2 -C8) branched or unbranched, --O-lower alkyl (C1 -C8) branched or unbranched, --O-lower alkenyl (C2 -C8) branched or unbranched, tetrahydrofuran, tetrahydrothiophene, or --CH2 --K wherein K is halogen, --OH, tetrahydrofuran, tetrahydrothiophene or the heterocyclic ring moiety: ##STR117## wherein D, E, F and G are selected from carbon or nitrogen and wherein the carbon atoms may be optionally substituted with halogen, (C1 -C3)lower alkyl, hydroxy, --CO-lower alkyl (C1 -C3), CHO, (C1 -C3)lower alkoxy, or --CO2 -lower alkyl (C1 -C3), and Ra and Rb are as hereinbefore defined;
(d) a moiety selected from those of the formulae: ##STR118## wherein Rc is selected from halogen, (C1 -C3) lower alkyl, --O-lower alkyl (C1 -C3) or OH;
Rb is as hereinbefore defined;
q is 1 or 2;
wherein Ar' is selected from the group: ##STR119## R7 is hydrogen, --CH3, --C2 H5, Cl, Br, F, --OCH3, --OC2 H5, or --CF3 ;
R8 and R9 are independently hydrogen, lower alkyl (C1 -C3), O-lower alkyl (C1 -C3), S-lower alkyl (C1 -C3), --CF3, --CN, --OH, --SCF3, --OCF3, halogen, NO2, amino, or --NH-lower alkyl (C1 -C3); R10 is selected from the group of halogen, hydrogen, or lower alkyl (C1 -C3);
W' is selected from O, S, NH, N-lower alkyl (C1 -C3), --NCO-lower alkyl (C1 -C3), or NSO2 -lower alkyl (C1 -C3);
the moiety ##STR120## represents a fused pyrazole ring or fused substituted pyrazole ring optionally substituted by one or two substituents selected from (C1 -C3) lower alkyl, halogen, formyl, (C1 -C3) lower alkoxy, --CO2 H, (C1 -C3) lower alkoxycarbonyl, ##STR121## a moiety of the formula: ##STR122## or a pharmaceutically acceptable salt, ester or prodrug thereof.
Within the group above are the following preferred sub-groups 1 and 2 of compounds:
1. wherein R3 is the moiety ##STR123## wherein Ar is a moiety selected from the group ##STR124## R6 is selected from the group ##STR125## or --CH2 COAr'; wherein
n is 0-2;
Ar' is ##STR126## ; W' is O or S;
A--B, Ra, Rb, R1, R2, R4, R5, R7, R8, R9, and cycloalkyl are as defined in Claim 1;
or a pharmaceutically acceptable salt, ester or prodrug thereof.
2. compounds of the formula: ##STR127## wherein; Y is a bond;
A--B is ##STR128## R1 is H, halogen (Cl, F, Br, I), OH, --S-lower alkyl (C1 -C3), --SH, --SO lower alkyl (C1 -C3), --SO2 lower alkyl (C1 -C3), --CO lower alkyl (C1 -C3), --CF3, lower alkyl (C1 -C3), --O lower alkyl (C1 -C3), --NO2, --NH2, --NHCO lower alkyl (C1 -C3), --N-[lower alkyl (C1 -C3)]2, SO2 NH2, --SO2 NH lower alkyl (C1 -C3), or --SO2 N [lower alkyl (C1 -C3)]2 ;
R2 is selected from H, Cl, Br, I, F, --OH, lower alkyl (C1 -C3), or --O lower alkyl (C1 -C3); or
R1 and R2 taken together are methylenedioxy or ethylenedioxy;
R3 is the moiety ##STR129## wherein Ar is a moiety selected from the group ##STR130## R5 is H, --CH3, --C2 H5, Cl, Br, F, --O--CH3, or --O--C2 H5 ;
R6 is selected from: ##STR131## wherein cycloalkyl is defined as C3 -C6 cycloalkyl, cyclohexenyl or cyclopentenyl;
n is 0-2;
and wherein Ar' is selected from the moieties: ##STR132## wherein Ra and Rb are independently selected from H, --CH3, or --C2 H5 ;
R7 is H, --CH3, --C2 H5, Cl, Br, F, --O--CH3, --O--C2 H5 or --CF3 ;
R8 and R9 are independently selected from hydrogen, lower alkyl (C1 -C3), O-lower alkyl (C1 -C3), S-lower alkyl (C1 -C3), --CF3, --CN, --OH, --SCF3, --OCF3, halogen, NO2, amino, or --NH-lower alkyl (C1 -C3);
W' is selected from O, S, NH, N-lower alkyl (C1 -C3), --NCO-lower alkyl (C1 -C3), or NSO2 -lower alkyl (C1 -C3);
the moiety ##STR133## represents a fused pyrazole ring or fused substituted pyrazole ring optionally substituted by one or two substituents selected from (C1 -C3) lower alkyl, halogen, formyl, (C1 -C3) lower alkoxy, --CO2 H, (C1 -C3) lower alkoxycarbonyl, ##STR134## a moiety of the formula: ##STR135## or a pharmaceutically acceptable salt, ester or prodrug thereof.
Among the more preferred compounds of this invention are those selected from:
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-methylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-chlorobenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2,4-dichlorobenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2,3-dichlorobenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-methyl-4-chlorobenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2,3-dimethylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-methoxybenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-(trifluoromethoxy)benzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2,4-dimethoxy)benzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-methoxy-4-chlorobenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d[1]benzazepin-6(2H)-yl)carbonyl]ph enyl]-2-(trifluoromethyl)benzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-3-(trifluoromethyl)benzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-(methylthio)benzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-4-fluoro-2-(trifluoromethyl)benzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d]1]benzazepin-6(2H)-yl)carbonyl]ph enyl]-2-fluoro-3-(trifluoromethyl)benzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-chloro-4-fluorobenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-3-fluoro-2-methylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-5-fluoro-2-methylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-3-fluoro-5-(trifluoromethyl)benzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-chloro-5-(methylthio)benzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-methyl-3-thiophenecarboxamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-methyl-3-furanecarboxamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-chlorobenzeneacetamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]p henyl]-2-methylbenzeneacetamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-chlorophenyl]-2-methylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-chlorophenyl]-2,3-dimethylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-chlorophenyl]-2,3-dichlorobenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-chlorophenyl]-2,4-dichlorobenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-chlorophenyl]-3-fluoro-2-methylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-chlorophenyl]-5-fluoro-2-methylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-chlorophenyl]-2-chloro-4-fluorobenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-methylphenyl]-2-methylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-methylphenyl]-2,3-dimethylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-methylphenyl]-2-chloro-4-fluorobenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-methylphenyl]-3-fluoro-2-methylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-methylphenyl]-5-fluoro-2-methylbenzamide.
N-[4-[(4,5-dihydro-2-methylpyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]- 3-methylphenyl]-2,4-dichlorobenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-chlorop henyl]-2-methylbenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-chlorop henyl]-2,3-dimethylbenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-chlorop henyl]-2,3-dichlorobenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-chlorop henyl]-2,4-dichlorobenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-chlorop henyl]-3-fluoro-2-methylbenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-chlorop henyl]-5-fluoro-2-methylbenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-chlorop henyl]-2-chloro-4-fluorobenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-methylp henyl]-2-methylbenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-methylp henyl]-2,3-dimethylbenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-methylp henyl]-2-chloro-4-fluorobenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-methylp henyl]-3-fluoro-2-methylbenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-methylp henyl]-5-fluoro-2-methylbenzamide.
N-[4-[(4,5-dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl]-3-methylp henyl]-2,4-dichlorobenzamide.
The subject compounds of the present invention are tested for biological activity as follows:
Rat liver plasma membranes expressing the vasopressin V1 receptor subtypes are isolated by sucrose density gradient according to the method described by Lesko et al., (1973). These membranes are quickly suspended in 50.0 mM Tris.HCl buffer, pH 7.4, containing 0.2% bovine serum albumin (BSA) and 0.1 mM phenylmethylsulfonylfluoride (PMSF) and kept frozen at -70°C until used in subsequent binding experiments. For binding experiments, the following is added to the wells of a ninety-six well format microtiter plate: 100 μl of 100.0 mM Tris.HCl buffer containing 10.0 mM MgCl2, 0.2% heat inactivated BSA and a mixture of protease inhibitors: leupeptin, 1.0 mg %; aprotinin, 1.0 mg %; 1,10-phenanthroline, 2.0 mg %; trypsin inhibitor, 10.0 mg % and 0.1 mM PMSF, 20.0 μl of [phenylalanyl-3,4,5,-3 H] vasopressin (S.A. 45.1 Ci/mmole) at 0.8 nM, and the reaction initiated by the addition of 80 μl of tissue membranes containing 20 μg of tissue protein. The plates are kept undisturbed on the bench top at room temperature for 120 min. to reach equilibrium. Non-specific samples are assayed in the presence of 0.1 μM of the unlabeled antagonist phenylalanylvasopressin, added in 20.0 μl volume.
For test compounds, these are solubilized in 50% dimethylsulfoxide (DMSO) and added in 20.0 μl volume to a final incubation volume of 200 μl. Upon completion of binding, the content of each well is filtered off, using a Brandel® cell Harvester (Gaithersburg, Md.). The radioactivity trapped on the filter disk by the ligand-receptor complex is assessed by liquid scintillation counting in a Packard LS Counter, with an efficiency of 65% for tritium. The data are analyzed for IC50 values by the LUNDON-2 program for competition (LUNDON SOFTWARE, OH) and displayed in Table I.
Medullary tissues from rat kidneys are dissected out, cut into small pieces and soaked in a 0.154 mM sodium chloride solution containing 1.0 mM EDTA with many changes of the liquid phase, until the solution is clear of blood. The tissue is homogenized in a 0.25M sucrose solution containing 1.0 mM EDTA and 0.1 mM PMSF using a Potter-Elvehjem homogenizer with a teflon pestle. The homogenate is filtered through several layers (4 layers) of cheese cloth. The filtrate is rehomogenized using a dounce homogenizer, with a tight fitting pestle. The final homogenate is centrifuged at 1500×g for 15 min. The nuclear pellet is discarded and the supernatant fluid recentrifuged at 40,000×g for 30 min. The resulting pellet formed contains a dark inner part with the exterior, slightly pink. The pink outer part is suspended in a small amount of 50.0 mM Tris.HCl buffer, pH 7.4. The protein content is determined by the Lowry's method (Lowry et al., J. Biol. Chem., 1953). The membrane suspension is stored at -70°C, in 50.0 mM Tris.HCl, containing 0.2% inactivated BSA and 0.1 mM PMSF in aliquots of 1.0 ml containing 10.0 mg protein per ml of suspension until use in subsequent binding experiments.
For binding experiments, the following is added in μl volume to wells of a 96 well format of a microtiter plate: 100.0 μl of 100.0 mM Tris.HCl buffer containing 0.2% heat inactivated BSA, 10.0 mM MgCl2 and a mixture of protease inhibitors: leupeptin, 1.0 mg %; aprotinin, 1.0 mg %; 1,10-phenanthroline, 2.0 mg %; trypsin inhibitor, 10.0 mg % and 0.1 mM PMSF, 20.0 μl of [3 H] Arginine8, vasopressin (S.A. 75.0 Ci/mmole) at 0.8 nM and the reaction initiated by the addition of 80.0 μl of tissue membranes (200.0 μg tissue protein). The plates are left undisturbed on the bench top for 120 min to reach equilibrium. Non-specific binding is assessed in the presence of 1.0 μM of unlabeled ligand, added in 20 μl volume. For test compounds, these are solubilized in 50% dimethylsulfoxide (DMSO) and added in 20.0 μl volume to a final incubation volume of 200 μl. Upon completion of binding, the content of each well is filtered off, using a Brandel® cell Harvester (Gaithersburg, Md.). The radioactivity trapped on the filter disk by the ligand-receptor complex is assessed by liquid scintillation counting in a Packard LS Counter, with an efficiency of 65% for tritium. The data are analyzed for IC50 values by the LUNDON-2 program for competition (LUNDON SOFTWARE, OH) and displayed in Table I.
(a) Platelet Membrane Preparation:
Frozen platelet rich plasma (PRP), received from the Hudson Valley Blood Services, are thawed to room temperature. (Platelet Source: Hudson Valley Blood Services, Westchester Medical Center, Valhalla, N.Y.). The tubes containing the PRP are centrifuged at 16,000×g for 10 min. at 4°C and the supernatant fluid discarded. The platelets resuspended in an equal volume of 50.0 mM Tris.HCl, pH 7.5 containing 120 mM NaCl and 20.0 mM EDTA. The suspension is recentrifuged at 16,000×g for 10 min. This washing step is repeated one more time. The wash discarded and the lysed pellets homogenized in low ionic strength buffer of Tris.HCl, 5.0 mM, pH 7.5 containing 5.0 mM EDTA. The homogenate is centrifuged at 39,000×g for 10 min. The resulting pellet is resuspended in Tris.HCl buffer, 70.0 mM, pH 7.5 and recentrifuged at 39,000×g for 10 min. The final pellet is resuspended in 50.0 mM Tris.HCl buffer pH 7.4 containing 120 mM NaCl and 5.0 mM KCl to give 1.0-2.0 mg protein per ml of suspension.
(b) Binding to Vasopressin V1 Receptor Subtype in Human Platelet Membranes:
In wells of a 96 well format microtiter plate, add 100 μl of 50.0 mM Tris.HCl buffer containing 0.2% BSA and a mixture of protease inhibitors (aprotinin, leupeptin etc.). Then add 20 μl of [3 H]Ligand (Manning or Arg8 Vasopressin), to give final concentrations ranging from 0.01 to 10.0 nM. Initiate the binding by adding 80.0 μl of platelet suspension (approx. 100 μg protein). Mix all reagents by pipetting the mixture up and down a few times. Non specific binding is measured in the presence of 1.0 μM of unlabeled ligand (Manning or Arg8 Vasopressin). Let the mixture stand undisturbed at room temperature for ninety (90) min. Upon this time, rapidly filter off the incubate under vacuum suction over GF/B filters, using a Brandel Harvester. The radioactivity caught on the filter disks is determined by the addition of liquid scintillant and counting in a liquid scintillator.
(a) Membrane Preparation
Flasks of 175 ml capacity, containing attached cells grown to confluence, are cleared of culture medium by aspiration. The flasks containing the attached cells are rinsed with 2×5 ml of phosphate buffered saline (PBS) and the liquid aspirated off each time. Finally, 5 ml of an enzyme free dissociation Hank's based solution (Specialty Media, Inc., Lafayette, N.J.) is added and the flasks are left undisturbed for 2 min. The content of all flasks is poured into a centrifuge tube and the cells pelleted at 300×g for 15 min. The Hank's based solution is aspirated off and the cells homogenized with a polytron at setting #6 for 10 sec in 10.0 mM Tris.HCl buffer, pH 7.4 containing 0.25M sucrose and 1.0 mM EDTA. The homogenate is centrifuged at 1500×g for 10 min to remove ghost membranes. The supernatant fluid is centrifuged at 100,000×g for 60 min to pellet the receptor protein. Upon completion, the pellet is resuspended in a small volume of 50.0 mM Tris.HCl buffer, pH 7.4. The protein content is determined by the Lowry method and the receptor membranes are suspended in 50.0 mM Tris.HCl buffer containing 0.1 mM phenylmethylsulfonylfluoride (PMSF) and 0.2% bovine serum albumin (BSA) to give 2.5 mg receptor protein per ml of suspension.
(b) Receptor Binding
For binding experiments, the following is added in μl volume to wells of a 96 well format of a microtiter plate: 100.0 μl of 100.0 mM Tris.HCl buffer containing 0.2% heat inactivated BSA, 10.0 mM MgCl2 and a mixture of protease inhibitors: leupeptin, 1.0 mg %; aprotinin, 1.0 mg %; 1,10-phenanthroline, 2.0 mg %; trypsin inhibitor, 10.0 mg % and 0.1 mM PMSF., 20.0 μl of [3 H] Arginine8, vasopressin (S.A. 75.0 Ci/mmole) at 0.8 nM and the reaction initiated by the addition of 80.0 μl of tissue membranes (200.0 pg tissue protein).
The plates are left undisturbed on the bench top for 120 min to reach equilibrium. Non specific binding is assessed in the presence of 1.0 μM of unlabeled ligand, added in 20 μl volume. For test compounds, these are solubilized in 50% dimethylsulfoxide (DMSO) and added in 20.0 μl volume to a final incubation volume of 200 μl. Upon completion of binding, the content of each well is filtered off, using a Brandel® cell Harvester (Gaithersburg, Md.). The radioactivity trapped on the filter disk by the ligand-receptor complex is assessed by liquid scintillation counting in a Packard LS Counter, with an efficiency of 65% for tritium. The data are analyzed for IC50 values by the LUNDON-2 program for competition (LUNDON SOFTWARE, OH) and the data is displayed in Table I.
| TABLE I |
| __________________________________________________________________________ |
| Binding Assey is Rat Hepatic V1 Receptors and Rat Kidney Medullary |
| V2 Receptors or *Binding to V1 |
| Receptor Subtype in Human Platelet and **Binding to Membranes of Mouse |
| Fibroblast Cell Line (LV-2) |
| Transfected with the cDNA Expressing the Human V2 Receptor |
| V1 |
| V2 |
| Ex. No. |
| Structure IC50 μM |
| IC50 μM |
| __________________________________________________________________________ |
| 1 |
| ##STR136## 2.5 0.86 |
| 2 |
| ##STR137## 0.15 0.068 |
| 3 |
| ##STR138## 0.17 0.42 |
| 4 |
| ##STR139## 1.5 1.7 |
| 5 |
| ##STR140## 0.056 0.029 |
| 6 |
| ##STR141## 60% (50 μM) |
| 80% (50 μM) |
| 7 |
| ##STR142## 0.63 0.077 |
| 8 |
| ##STR143## 2.8 1.5 |
| 9 |
| ##STR144## 1.1 0.28 |
| 10 |
| ##STR145## 0.17 0.064 |
| 11 |
| ##STR146## 0.16 0.033 |
| 12 |
| ##STR147## 0.39 0.18 |
| 13 |
| ##STR148## 0.34 0.30 |
| 14 |
| ##STR149## 0.057 0.066 |
| 16 |
| ##STR150## 0.053 0.065 |
| 17 |
| ##STR151## 65% (50 μM) |
| 52% (50 μM) |
| 18 |
| ##STR152## 0.084 0.52 |
| 19 |
| ##STR153## 1.3 1.2 |
| 20 |
| ##STR154## 17% (10 μM) |
| 48% (10 μM) |
| 21 |
| ##STR155## 0.28 0.44 |
| 22 |
| ##STR156## 0.32 0.14 |
| 23 |
| ##STR157## 19% (50 μM) |
| 47% (50 μM) |
| 24 |
| ##STR158## 0.17 0.41 |
| 25 |
| ##STR159## 0.052 0.56 |
| 61 |
| ##STR160## 0.052 0.068 |
| 62 |
| ##STR161## 0.404 0.858 |
| 92 |
| ##STR162## 0.098 0.025 |
| 119 |
| ##STR163## 0.039 1.1 |
| 121 |
| ##STR164## 72% (1 μM) |
| 80% (1 μM) |
| 122 |
| ##STR165## *95% (10 μM) |
| *92% (10 μM) |
| 123 |
| ##STR166## 0.043 0.19 |
| 124 |
| ##STR167## 1.2 0.21 |
| 136 |
| ##STR168## 0.016 0.027 |
| 137 |
| ##STR169## 0.076 0.064 |
| 138 |
| ##STR170## 0.046 0.051 |
| 167 |
| ##STR171## 0.51 0.43 |
| 165 |
| ##STR172## 0.49 0.37 |
| 166 |
| ##STR173## 0.083 0.245 |
| 249 |
| ##STR174## 61% (10 μM) |
| 88% (10 μM) |
| 250 |
| ##STR175## 62% (10 μM) |
| 77% (10 μM) |
| 251 |
| ##STR176## 1.5 0.14 |
| 252 |
| ##STR177## 0.26 0.26 |
| 253 |
| ##STR178## 0.31 0.19 |
| 254 |
| ##STR179## 0.10 0.037 |
| 255 |
| ##STR180## 0.027 0.039 |
| 256 |
| ##STR181## 0.10 0.054 |
| 257 |
| ##STR182## *0.016 **0.020 |
| 258 |
| ##STR183## 75% (10 μM) |
| 87% (10 μM) |
| 259 |
| ##STR184## 4.0 13% (10 μM) |
| 260 |
| ##STR185## 0.12 0.065 |
| 261 |
| ##STR186## *0.021 **0.016 |
| 262 |
| ##STR187## *0.62 **0.25 |
| 263 |
| ##STR188## *0.43 **0.047 |
| 264 |
| ##STR189## *0.98 **0.028 |
| 267 |
| ##STR190## 0.026 0.082 |
| 268 |
| ##STR191## 0.019 0.042 |
| 345 |
| ##STR192## 99% (10 μM) |
| 81% (10 μM) |
| 379 |
| ##STR193## 0.25 0.053 |
| 416 |
| ##STR194## 34.5 1.1 |
| 448 |
| ##STR195## 0.073 0.19 |
| 449 |
| ##STR196## 0.36 0.25 |
| 453 |
| ##STR197## *14% (10 μM) |
| 26% (10 μM) |
| 456 |
| ##STR198## *0.036 **0.009 |
| 457 |
| ##STR199## *0.039 **0.012 |
| 458 |
| ##STR200## *0.0018 |
| **0.00071 |
| 454 |
| ##STR201## 64% (10 μM) |
| 33% (10 μM) |
| 454 |
| ##STR202## 64% (10 μM) |
| 37% (10 μM) |
| 451 |
| ##STR203## *35% (1 μM) |
| **69% (1 μM) |
| 450 |
| ##STR204## 87% (10 μM) |
| 29% (10 μM) |
| 452 |
| ##STR205## *0.47 **0.01 |
| 455 |
| ##STR206## *0.25 **0.10 |
| 534 |
| ##STR207## *0.09 **0.073 |
| 581 |
| ##STR208## *0.15 0.74 |
| **0.0022 0.0113 |
| 582 |
| ##STR209## 1.3 0.0053 |
| 583 |
| ##STR210## 0.082 0.040 |
| 584 |
| ##STR211## *0.028 0.039 |
| 0.011 |
| 585 |
| ##STR212## *0.012 0.032 |
| __________________________________________________________________________ |
Conscious hydrated rats are treated with compounds under study from 0.1 to 100 mg/kg orally or vehicle. Two to four rats are used for each compound. One hour later, arginine vasopressin (AVP, antidiuretic hormore, ADH) dissolved in peanut oil is administered at 0.4 μg/kg intraperitoneally. Two rats in each test would not receive arginine vasopressin but only the vehicle (peanut oil) to serve as water-loading control. Twenty minutes later each rat is given 30 mL/kg of deionized water orally by gavage and is placed individually in a metabolic cage equipped with a funnel and a graduated glass cylinder to collect urine for four hours. Urine volume is measured and osmolality analyzed by use of a Fiske One-Ten osmometer (Fiske Assoc., Norwood, Mass., USA). Urinary sodium, potassium, and chloride are analyzed by use of ion-specific electrodes in a Beckman E3 (Electrolyte 3) Analyzer. In the following results, decreased urine volume and decreased osmolality relative to AVP-control indicates activity. The results are displayed in Table II.
| TABLE II |
| ______________________________________ |
| Diuretic Effect of CL Compounds (V2 Antagonism) |
| in Normal Sprague-Dawley Rats |
| Dose Urine Volume |
| Osmolality |
| Ex. No. (mg/kg) N (ml/4 hrs.) |
| (mOsm/kg) |
| ______________________________________ |
| Water-Load |
| Control 78 13.3 ± 0.3 |
| 229 ± 6 |
| Water-Load |
| Control + |
| (10%) 6 12.1 ± 1 |
| 497 ± 53 |
| DMSO (20%) 4 12.4 ± 0.8 |
| 361 ± 30 |
| AVP-Control 76 2 ± 0.2 |
| 1226 ± 58 |
| 2 30 6 18.9 ± 1.7 |
| 401 ± 49 |
| 10 6 15.2 ± 2.4 |
| 408 ± 34 |
| 3 6 2.9 ± 0.5 |
| 1235 ± 162 |
| 5 10 2 2 1137 |
| 10 10 2 7.1 1023 |
| 11 30 4 9.7 ± 0.6 |
| 1114 ± 55 |
| 14 30 2 13.1 739 |
| 16 30 2 7.2 1257 |
| 22 30 2 10.2 738 |
| 61 10 2 9.3 975 |
| 62 10 2 7.7 1034 |
| 92 10 2 13.5 679 |
| 121 10 2 5 1064 |
| 123 10 2 5.8 1125 |
| 124 10 2 5.5 1172 |
| 136 30 2 19 306 |
| 137 30 2 15.8 358 |
| 138 30 2 13.8 380 |
| 167 30 2 2 1544 |
| 250 30 2 6.5 1378 |
| 251 10 2 7.5 835 |
| 252 10 2 7.5 790 |
| 253 10 2 6 837 |
| 255 30 2 2.3 1456 |
| 257 10 2 11.8 434 |
| 259 10 2 6 1080 |
| 260 10 4 13.8 ± 2.1 |
| 498 ± 25 |
| 261 10 2 3.8 1337 |
| 262 10 2 6.8 899 |
| 264 10 2 12 682 |
| 266 10 2 4.3 1186 |
| 267 10 2 7.5 517 |
| 268 30 2 7.4 831 |
| 379 20 2 7 483 |
| 416 10 2 4.3 1156 |
| 448 30 2 4.8 1084 |
| 451 10 2 5.3 989 |
| 452 10 2 7.8 584 |
| 453 10 2 9.4 776 |
| 454 10 2 4 1255 |
| 455 10 2 13.5 420 |
| 456 10 2 4.8 1209 |
| 457 10 2 5.8 1036 |
| 458 10 2 13.5 537 |
| 534 10 2 10.6 695 |
| ______________________________________ |
Conscious rats are restrained in a supine position with elastic tape. The area at the base of the tail is locally anesthetized by subcutaneous infiltration with 2% procaine (0.2 ml). Using aseptic technique the ventral caudal tail artery is isolated and a cannula made of PE 10 and 20 (heat-fused) tubing is passed into the lower abdominal aorta. The cannula is secured, heparinized (1000 i.u./cc), sealed and the wound closed with one or two stitches of Dexon 4-0. The caudal vein is also cannulated in the same manner for intravenous drug administration. The duration of the surgery is approximately 5 minutes. Additional local anesthesia (2% procaine or lidocaine) is provided as needed.
The animals are placed in plastic restraining cages in an upright position. The cannula is attached to a Statham P23Db pressure transducer and pulsatile blood pressure is recorded. Increase of systolic blood pressure responses to arginine vasopressin 0.01 and 0.2 international unit (I.U.)(350 I.U.=1 mg) injections are recorded prior to any drug (compound) administration, after which each rat is dosed orally with compounds under study 0.1-100 mg/kg (10 cc/kg) or intravenously 0.1-30 mg/kg (1 cc/kg). The vasopressin injections are repeated 30, 60, 90, 120, 180, 240 and 300 min. later. Percentage of antagonism by the compound is calculated using the pre-drug vasopressin vasopressor response as 100%.
The results of this test on representative compounds of this invention are shown in Table III.
The results of this test on representative compounds of this invention in which the dose, the maximum % inhibition and the time in minutes, are shown in Table IV.
| TABLE III |
| __________________________________________________________________________ |
| VASOPRESSIN (VAS) VASOPRESSOR RESPONSE |
| VAS Dose |
| Dose i.u./kg |
| Min Post |
| Control |
| Response |
| Average |
| (mg/kg) I.V. Dose Before VAS |
| After VAS |
| Change |
| Change |
| Inhibition |
| __________________________________________________________________________ |
| CONTROL 0.05 0 240 270 30 27.5 |
| 230 255 25 |
| 0.1 230 285 55 45 |
| 235 270 35 |
| Ex. No. 5 |
| 10 i.v. |
| 0.05 30 225 260 35 30 -9 |
| 235 260 25 |
| 0.1 230 265 35 35 22 |
| 225 260 35 |
| Ex. No. 5 |
| 20 i.v. |
| 0.05 60 245 250 5 10 64 |
| 225 240 15 |
| 0.1 245 260 15 17.5 61 |
| 235 255 20 |
| 0.05 90 225 240 15 10 64 |
| 220 225 5 |
| 0.1 230 250 20 56 |
| 225 245 20 |
| 0.05 120 225 245 20 15 45 |
| 225 235 10 |
| 0.1 230 245 15 17.5 61 |
| 225 245 20 |
| 0.05 180 225 245 20 12.5 55 |
| 230 235 5 |
| 0.1 225 255 30 20 56 |
| 240 250 10 |
| 0.05 240 215 235 20 17.5 36 |
| 220 235 15 |
| 0.1 215 250 35 25 44 |
| 230 245 15 |
| 0.05 300 210 245 35 22.5 18 |
| 245 255 10 |
| 0.1 200 235 35 30 33 |
| 235 260 25 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight (s): 400, 400 grams |
| CONTROL 0.01 0 130 170 40 30 |
| 159 179 20 |
| 0.02 136 192 56 51 |
| 164 210 46 |
| Ex. No. 10 |
| 10 i.v. |
| 0.01 30 136 184 48 37.5 -25 |
| 150 177 27 |
| 0.02 136 197 61 64.5 -26 |
| 150 218 68 |
| Ex. No. 10 |
| 20 i.v. |
| 0.01 60 141 156 15 14.5 52 |
| 150 164 14 |
| 0.02 138 161 23 22.5 56 |
| 152 174 22 |
| 0.01 90 137 154 17 16 ,47 |
| 151 166 15 |
| 0.02 135 161 26 23 55 |
| 153 173 20 |
| 0.01 120 138 155 17 13.5 55 |
| 150 160 10 |
| 0.02 138 161 23 22 57 |
| 157 178 21 |
| 0.01 180 132 154 22 25.5 15 |
| 145 174 29 |
| 0.02 140 174 34 29.5 42 |
| 157 182 25 |
| 0.01 240 131 156 25 22.5 25 |
| 149 169 20 |
| 0.02 140 174 34 34.5 32 |
| 157 192 35 |
| 0.01 300 144 166 22 24.5 18 |
| 151 178 27 |
| 0.02 135 170 35 34 33 |
| 145 178 33 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 520, 470 grams |
| CONTROL 0.01 0 225 245 20 20 |
| 205 225 20 |
| 0.02 225 255 30 32.5 |
| 205 240 35 |
| Vehicle |
| 10 cc/kg po. |
| 0.01 30 215 240 25 25 -25 |
| 2% starch 200 225 25 |
| 0.02 220 255 35 35 -8 |
| 210 245 35 |
| 0.01 60 220 240 20 25 -25 |
| 200 230 30 |
| 0.02 225 260 35 35 -8 |
| 215 250 35 |
| 0.01 90 210 235 25 22.5 -13 |
| 200 220 20 |
| 0.02 235 275 40 37.5 -15 |
| 205 240 35 |
| 0.01 120 225 245 20 22.5 -13 |
| 200 225 25 |
| 0.02 225 250 25 35 -8 |
| 200 245 45 |
| 0.01 180 210 235 25 20 0 |
| 195 210 15 |
| 0.02 225 255 30 36 -11 |
| 195 237 42 |
| 0.01 240 225 240 15 22.5 -13 |
| 180 210 30 |
| 0.02 225 250 25 35 -8 |
| 190 235 45 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 420, 420 grams |
| CONTROL 0.01 0 135 152 17 22.5 |
| 132 160 28 |
| 0.02 149 216 67 52 |
| 137 174 37 |
| Ex. No. 14 |
| 10 i.v. |
| 0.01 30 131 142 11 11 51 |
| 143 154 11 |
| 0.02 151 173 22 23 56 |
| 136 160 24 |
| 0.01 60 151 163 12 12 47 |
| 139 151 12 |
| 0.02 153 171 18 22 58 |
| 140 166 26 |
| 0.01 90 135 142 7 13.5 40 |
| 140 160 20 |
| 0.02 135 173 38 29.5 43 |
| 145 166 21 |
| 0.01 120 137 155 18 16 29 |
| 137 151 14 |
| 0.02 143 168 25 23.5 55 |
| 139 161 22 |
| 0.01 180 131 153 22 21.5 4 |
| 134 155 21 |
| 0.02 140 170 30 30.5 41 |
| 131 162 31 |
| 0.01 240 146 170 24 26.5 -18 |
| 141 166 29 |
| 0.02 140 171 30 32 38 |
| 141 175 34 |
| 0.01 300 138 160 22 24.5 -9 |
| 142 169 27 |
| 0.02 152 186 34 33 37 |
| 144 176 32 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 480, 420 grams |
| CONTROL 0.01 0 139 182 43 29.5 |
| 128 144 16 |
| 0.02 142 182 40 33 |
| 162 188 26 |
| Ex. No. 16 |
| 30 p.o. |
| 0.01 30 133 166 33 25 15 |
| 148 165 17 |
| 0.02 136 163 27 27.5 17 |
| 154 182 28 |
| 0.01 60 131 168 37 30.5 -3 |
| 154 178 24 |
| 0.02 134 163 29 29 12 |
| 162 191 29 |
| 0.01 90 137 166 29 28.5 3 |
| 149 177 28 |
| 0.02 137 172 35 40.5 -23 |
| 149 195 46 |
| 0.01 120 146 163 17 23.5 20 |
| 148 178 30 |
| 0.02 142 166 24 29.5 11 |
| 161 196 35 |
| 0.01 180 142 169 27 30. 5 |
| -3 |
| 140 174 34 |
| 0.02 138 169 31 26 21 |
| 153 174 21 |
| 0.01 240 140 163 23 30 -2 |
| 140 177 37 |
| 0.02 143 160 17 30 9 |
| 157 200 43 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 420, 460 grams |
| CONTROL 0.01 0 147 169 22 21.5 |
| 138 159 21 |
| 0.02 151 198 47 42 |
| 145 182 37 |
| Ex. No. 22 |
| 10 i.v. |
| 0.01 30 152 164 12 8.5 60 |
| 146 151 5 |
| 0.02 152 177 25 18.5 56 |
| 148 160 12 |
| 0.01 60 151 166 15 11 49 |
| 144 151 7 |
| 0.02 121 144 23 25 40 |
| 141 168 27 |
| 0.01 90 149 166 17 14 35 |
| 140 151 11 |
| 0.02 151 166 15 13 69 |
| 140 151 11 |
| 0.01 120 138 155 17 17.5 19 |
| 129 147 18 |
| 0.02 148 183 35 30 29 |
| 141 166 25 |
| 0.01 180 142 168 26 20 7 |
| 141 155 14 |
| 0.02 150 185 35 36 14 |
| 132 169 37 |
| 0.01 240 134 169 35 28 -30 |
| 139 160 21 |
| 0.02 139 200 61 48 -14 |
| 150 185 35 |
| 0.01 300 139 171 32 30 -40 |
| 121 149 28 |
| 0.02 140 210 70 52.5 -25 |
| 150 185 35 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 450, 495 grams |
| CONTROL 0.01 3 0 161 191 30 28.5 |
| 143 170 27 |
| 0.02 157 217 60 51.5 |
| 145 188 43 |
| Ex. No. 61 |
| 10 i.v. |
| 0.01 30 158 182 24 21 26 |
| 146 164 18 |
| 0.02 158 196 38 32.5 37 |
| 146 173 27 |
| Ex. No. 61 |
| 20 i.v. |
| 0.01 60 163 180 17 13 54 |
| 147 156 9 |
| 0.02 159 179 20 14.5 72 |
| 146 155 9 |
| 0.01 90 153 165 12 8 72 |
| 150 154 4 |
| 0.02 154 173 19 16.5 68 |
| 144 158 14 |
| 0.01 120 151 165 14 12 58 |
| 145 155 10 |
| 0.02 151 176 25 20 61 |
| 143 158 15 |
| 0.01 180 142 165 23 15.5 46 |
| E |
| 143 151 8 |
| 0.02 148 172 24 17.5 66 |
| 145 156 11 |
| 0.01 240 144 156 12 12 58 |
| 144 156 12 |
| 0.02 150 179 29 23 55 |
| 147 164 17 |
| 0.01 300 142 161 19 15.5 46 |
| 144 156 12 |
| 0.02 140 167 27 24 53 |
| 153 174 21 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 570, 460 grams |
| CONTROL 0.01 0 139 179 40 39.5 |
| 150 189 39 |
| 0.02 143 204 61 60.5 |
| 156 216 60 |
| Ex. No. 62 |
| 10 i.v. |
| 0.01 30 144 196 52 57 -44 |
| 141 203 62 |
| 0.02 158 217 59 61.5 -2 |
| 163 227 64 |
| Ex. No. 62 |
| 20 i.v. |
| 0.01 60 131 148 17 13 67 |
| 135 144 9 |
| 0.02 135 173 38 29. 5 |
| 51 |
| 134 155 21 |
| 0.01 90 132 156 24 23 42 |
| 127 149 22 |
| 0.02 140 195 55 54 11 |
| 136 189 53 |
| 0.01 120 137 160 23 23.5 41 |
| 138 162 24 |
| 0.02 143 189 46 50 17 |
| 139 193 54 |
| 0.01 180 131 155 24 24.5 38 |
| 133 158 25 |
| 0.02 139 155 16 15 75 |
| 144 158 14 |
| 0.01 240 126 153 27 30 24 |
| 133 166 33 |
| 0.02 134 167 33 40.5 33 |
| 142 190 48 |
| 0.01 300 128 152 24 25 37 |
| 138 164 26 |
| 0.02 140 167 27 38 37 |
| 146 195 49 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 500, 450 grams |
| CONTROL 0.01 0 144 177 33 30 |
| 128 155 27 |
| 0.02 145 197 52 46.5 |
| 127 168 41 |
| Ex. No. 92 |
| 10 i.v. |
| 0.01 30 155 165 10 10.5 65 |
| 153 164 11 |
| 0.02 160 177 17 18.5 60 |
| 155 175 20 |
| 0.01 60 143 159 16 18 40 |
| 150 170 20 |
| 0.02 152 168 16 22 53 |
| 149 177 28 |
| 0.01 90 145 162 17 17.5 42 |
| 127 145 18 |
| 0.02 141 168 27 27 42 |
| 144 171 27 |
| 0.01 120 135 156 21 19 37 |
| 113 130 17 |
| 0.02 134 154 20 20.5 56 |
| 126 147 21 |
| 0.01 1B0 137 159 22 18 40 |
| 144 158 14 |
| 0.02 144 180 36 29.5 37 |
| 142 165 23 |
| 0.01 240 149 169 20 19 37 |
| 137 155 18 |
| 0.02 149 169 20 18 61 |
| 146 162 16 |
| 0.01 300 151 171 20 17.5 42 |
| 144 159 15 |
| 0.02 158 182 24 24 48 |
| 139 163 24 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 440, 490 grams |
| CONTROL 0.01 0 149 177 28 28 |
| 149 177 28 |
| 0.02 155 196 41 41 |
| 155 196 41 |
| Ex. No. 121 |
| 10 i.v. |
| 0.01 30 154 175 21 21 25 |
| 154 175 21 |
| 0.02 154 190 36 36 12 |
| 154 190 36 |
| Ex. No. 121 |
| 20 i.v. |
| 0.0i 60 155 168 13 13 54 |
| 155 168 13 |
| 0.02 156 175 19 19 54 |
| 156 175 19 |
| 0.01 90 159 172 13 13 54 |
| 159 172 13 |
| 0.02 157 179 22 22 46 |
| 157 179 22 |
| 0.01 120 159 164 5 5 82 |
| 159 164 5 |
| 0.02 154 173 19 19 54 |
| 154 173 19 |
| 0.01 180 151 169 18 18 36 |
| 151 169 18 |
| 0.02 159 173 14 14 66 |
| 159 173 14 |
| 0.01 240 149 169 20 20 29 |
| 149 169 20 |
| 0.02 151 172 21 21 49. |
| 151 172 21 |
| 0.01 300 151 168 17 17 39 |
| 151 168 17 |
| 0.02 152 177 25 25 39 |
| 152 177 25 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 585, 410 grams |
| CONTROL 0.01 0 140 162 22 17.5 |
| 14:7 160 13 |
| 0.02 138 169 31 33 |
| 141 176 35 |
| Ex. No. 123 |
| 10 i.v. |
| 0.0i 30 139 151 12 17.5 0 |
| 154 177 23 |
| 0.02 139 177 38 20 39 |
| 154 156 2 |
| Ex. No. 123 |
| 20 i.v. |
| 0.01 60 137 i40 3 6 66 |
| 147 156 9 |
| 0.02 132 139 7 6 82 |
| 146 151 5 |
| 0.01 90 137 141 4 6 66 |
| 149 157 |
| 0.02 135 138 3 7 79 |
| 149 160 11 |
| 0.01 120 136 139 3 5 71 |
| 147 154 7 |
| 0.02 138 141 3 4.5 86 |
| 150 156 6 |
| 0.01 180 138 141 3 4 77 |
| 150 155 5 |
| 0.02 138 142 4 6.5 80 |
| 148 157 9 |
| 0.01 240 137 145 8 8 54 |
| 146 154 8 |
| 0.02 140 146 6 6.5 80 |
| 148 155 7 |
| 0.01 300 138 144 6 8 54 |
| 144 154 10 |
| 0.02 140 148 8 8 76 |
| 146 154 8 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 500, 480 grams |
| CONTROL 0.01 0 150 172 22 32.5 |
| 140 183 43 |
| 0.02 147 187 40 35.5 |
| 162 193 31 |
| Ex. No.124 |
| 10 i.v. |
| 0.01 30 150 187 37 38.5 -1B |
| 153 193 40 |
| 0.02 148 187 39 43.5 -23 |
| 146 194 48 |
| Ex. No. 124 |
| 20 i.v. |
| 0.01 60 151 155 4 4 88 |
| 148 152 4 |
| 0.02 150 173 23 17.5 51 |
| 147 159 12 |
| 0.01 90 143 159 16 12.5 62 |
| 147 156 9 |
| 0.02 148 163 15 18 49 |
| 144 165 21 |
| 0.01 120 144 157 13 12.5 62 |
| 147 159 12 |
| 0.02 151 167 16 9.5 45 |
| 149 172 23 |
| 0.01 180 131 152 21 21 35 |
| 125 146 21 |
| 0.02 143 165 22 21.5 39 |
| 143 164 21 |
| 0.01 240 144 163 19 18 45 |
| 141 158 17 |
| 0.02 147 168 21 24.5 31 |
| 148 176 28 |
| 0.01 300 141 160 19 20 38 |
| 138 159 21 |
| 0.02 139 176 37 33.5 6 |
| 142 172 30 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 600, 550 grams |
| CONTROL 0.01 0 230 265 35 25 |
| 235 250 15 |
| 0.02 240 280 40 35 |
| 235 265 30 |
| Ex. No. 5 |
| 50 p∅ |
| 0.01 30 250 275 25 22.5 10 |
| 240 260 20 |
| 0.02 260 295 35 35 0 |
| 235 270 35 |
| 0.01 60 240 275 35 30 -20 |
| 235 260 25 |
| 0.02 260 290 30 35.5 7 |
| 245 280 35 |
| 0.01 90 255 270 15 17.5 30 |
| 235 255 20 |
| 0.02 260 290 30 32.5 7 |
| 245 280 35 |
| 0.01 120 245 285 40 30 -20 |
| 220 240 20 |
| 0.02 260 295 35 40 -14 |
| 255 270 45 |
| 0.01 180 240 255 15 20 20 |
| 220 245 25 |
| 0.02 240 270 30 37.5 -7 |
| 250 295 45 |
| 0.01 240 230 255 25 22.5 10 |
| 255 245 20 |
| 0.02 260 290 30 38.5 -10 |
| 233 280 47 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 410, 430 grams |
| CONTROL 0.05 0 240 270 30 27.5 |
| 230 255 25 |
| 0.1 230 285 55 45 |
| 235 270 35 |
| Ex. No. 5 |
| 10 i.v. |
| 0.05 30 225 260 35 30 -9 |
| 235 260 25 |
| 0.01 230 265 35 35 22 |
| 225 260 35 |
| 20 i.v. |
| 0.05 60 245 250 5 10 64 |
| 225 240 15 |
| 0.01 245 260 15 17.5 61 |
| 235 255 20 |
| 0.05 90 225 240 15 10 64 |
| 220 225 5 |
| 0.1 230 250 20 20 56 |
| 225 245 20 |
| 0.05 120 225 245 20 15 45 |
| 225 235 10 |
| 0.1 230 245 15 17.5 61 |
| 225 245 20 |
| 0.05 180 225 245 20 12. 5 |
| 55 |
| 230 235 5 |
| 0.1 225 255 30 20 56 |
| 240 250 10 |
| 0.05 240 215 235 20 17.5 36 |
| 220 235 15 |
| 0.1 215 250 35 25 44 |
| 230 245 15 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 400, 400 grams |
| 0.05 300 210 245 35 22.5 18 |
| 245 255 10 |
| 0.01 200 235 35 30 33 |
| 235 260 25 |
| CONTROL 0.01 0 130 170 40 30 |
| 159 179 20 |
| 0.02 136 192 56 51 |
| 164 210 46 |
| Ex. No.10 |
| 10 i.v. |
| 0.01 30 136 184 48 37.5 -25 |
| 150 177 27 |
| 0.02 136 197 61 64.5 -26 |
| 150 218 68 |
| 20 i.v. |
| 0.01 60 141 156 15 14.5 52 |
| 150 164 14 |
| 0.02 138. 161 23 22.5 56 |
| 152 174 22 |
| 0.01 90 137 154 17 16 47 |
| 151 166 15 |
| 0.02 135 161 26 23 55 |
| 153 173 20 |
| 0.01 120 138 155 17 13.5 55 |
| 150 160 10 |
| 0.02 138 161 23 22 57 |
| 157 178 21 |
| 0.01 180 132 154 22 25.5 15 |
| 145 174 29 |
| 0.02 140 174 34 29.5 42 |
| 157 182 25 |
| 0.01 240 131 156 25 22.5 25 |
| 149 169 20 |
| 0.02 140 174 34 34.5 32 |
| 157 192 35. |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 520, 470 grams |
| 0.01 300 144 166 22 24.5 18 |
| 151 178 27 |
| 0.02 135 170 35 34 33 |
| 145 178 33 |
| CONTROL 0.01 0 135 152 17 22.5 |
| 132 160 28 |
| 0.02 149 216 67 52 |
| 137 174 37 |
| Ex. No.14 |
| 10 i.v. |
| 0.01 30 131 142 11 51 |
| 143 154 |
| 0.02 151 173 22 23 56 |
| 136 160 24 |
| 0.01 60 151 163 12 12 47 |
| 139 151 12 |
| 0.02 153 171 18 22 58 |
| 140 166 26 |
| 0.01 90 135 142 7 13.5 40 |
| 140 160 20 |
| 0.02 135 173 38 29.5 43 |
| 145 166 21 |
| 0.01 120 137 155 18 16 29 |
| 137 151 14 |
| 0.02 143 168 25 23.5 55 |
| 139 161 22 |
| 0.01 180 131 153 22 21.5 4 |
| 134 155 21 |
| 0.02 140 170 30 30.5 41 |
| 131 162 31 |
| 0.01 240 146 170 24 26.5 -18 |
| 141 166 29 |
| 0.02 140 171 30 32 38 |
| 141 175 .34 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 480, 420 grams |
| 0.01 300 138 160 22 24.5 -9 |
| 142 169 27 |
| 0.02 152 186 34 33 37 |
| 144 176 32 |
| CONTROL 0.01 0 139 182 43 29.5 |
| 128 144 16 |
| 0.02 142 182 40 33 |
| 162 188 26 |
| Ex. No.16 |
| 30 p.o. |
| 0.01 30 133 166 33 25 15 |
| 148 165 17 |
| 0.02 136 163 27 27.5 17 |
| 154 182 28 |
| 0.01 60 131 168 37 30.5 -3 |
| 154 178 24 |
| 0.02 134 163 29 29 12 |
| 162 191 29 |
| 0.01 90 137 166 29 28.5 3 |
| 149 177 28 |
| 0.02 137 172 35 40.5 -23 |
| 149 195 46 |
| 0.01 120 146 163 17 23.5 20 |
| 148 178 30 |
| 0.02 142 166 24 29.5 11 |
| 161 196 35 |
| 0.01 180 142 169 27 30.5 -3 |
| 140 174 34 |
| 0.02 138 169 31 26 21 |
| 153 174 21 |
| 0.01 240 140 163 23 30 -2 |
| 140 177 37 |
| 0.02 143 160 17 30 9 |
| 157 200 43 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 420, 460 grams |
| CONTROL 0.01 0 147 169 22 21.5 |
| 138 159 21 |
| 0.02 151 198 47 42 |
| 145 182 37 |
| Ex. No. 22 |
| 10 i.v. |
| 0.0i 30 152 164 12 8.5 60 |
| 146 151 5 |
| 0.02 152 177 25 18.5 56 |
| 148 160 12 |
| 0.01 60 151 166 15 11 49 |
| 144 151 7 |
| 0.02 121 144 23 25 40 |
| 141 168 27 |
| 0.01 90 149 166 17 14 35 |
| 140 151 11 |
| 0.02 151 166 15 13 69 |
| 140 151 11 |
| 0.01 120 138 155 17 17.5 19 |
| 129 147 18 |
| 0.02 148 183 35 30 29 |
| 141 166 25 |
| 0.01 180 142 168 26 20 7 |
| 141 155 14 |
| 0.02 150 185 35 36 14 |
| 132 169 37 |
| 0.01 240 134 169 35 28 -30 |
| 139 160 21 |
| 0.02 139 200 61 48 -14 |
| 150 185 35 |
| 0.01 300 139 *171 32 30 -40 |
| 121 149 28 |
| 0.02 140 210 70 *52.5 |
| -25 |
| 150 185 35 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 450, 495 grams |
| CONTROL 0.01 0 161 191 30 28.5 |
| 143 170 27 |
| 0.02 157 217 60 51.5 |
| 145 . 188 43 |
| Ex. No. 61 |
| 10 i.v. |
| 0.0i 30 158 162 24 21 26 |
| 146 164 18 |
| 0.02 158 196 38 32.5 37 |
| 146 173 27 |
| 20 i.v. |
| 0.01 60 163 180 17 13 54 |
| 147 156 9 |
| 0.02 159 179 20 14.5 72 |
| 146 155 9 |
| 0.01 90 153 165 12 8 72 |
| 150 154 4 |
| 0.02 154 173 19 16.5 68 |
| 144 158 14 |
| 0.01 120 151 165 14 12 58 |
| 145 155 10 |
| 0.02 151 176 25 20 61 |
| 143 158 15 |
| 0.01 180 142 165 23 15.5 46 |
| 143 151 8 |
| 0.02 148 172 24 17.5 66 |
| 145 156 11 |
| 0.01 240 144 156 12 12 58 |
| 144 156 12 |
| 0.02 150 179 29 23 55 |
| 147 164 17 |
| 0.01 300 142 161 19 15.5 46 |
| 144 156 12 |
| 0.02 140 167 27 24 |
| 153 174 21 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 570, 460 grams |
| CONTROL 0.01 0 139 179 40 39.5 |
| 150 189 39 |
| 0.02 143 204 61 60.5 |
| 156 216 60 |
| Ex. No. 62 |
| 10 i.v. |
| 0.01 30 144 196 52 57 -44 |
| 141 203 62 |
| 0.02 158 217 59 61.5 -2 |
| 163 227 64 |
| Ex. No. 62 |
| 20 i.v. |
| 0.01 60 131 148 17 13 67 |
| 135 144 9 |
| 0.02 135 173 38 29. 5 |
| 51 |
| 134 155 21 |
| 0.01 90 132 156 24 23 42 |
| 127 149 22 |
| 0.02 140 195 55 54 11 |
| 136 189 53 |
| 0.01 120 137 160 23 23.5 41 |
| 138 162 24 |
| 0.02 143 189 46 50 17 |
| 139 193 54 |
| 0.01 180 131 155 24 24.5 38 |
| 133 158 25 |
| 0.02 139 155 16 15 75 |
| 144 158 14 |
| 0.01 240 126 153 27 30 24 |
| 133 166 33 |
| 0.02 134 167 33 40.5 33 |
| 142 190 48 |
| 0.01 300 128 152 24 25 37 |
| 138 164 26 |
| 0.02 140 167 27 38 37 |
| 146 195 49 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 500, 450 grams |
| CONTROL 0.01 0 144 177 33 30 |
| 128 155 27 |
| 0.02 145 197 52 46.5 |
| 127 168 41 |
| Ex. No. 92 |
| 10 i.v. |
| 0.01 30 155 165 10 10.5 65 |
| 153 164 11 |
| 0.02 160 177 17 18.5 60 |
| 155 175 20 |
| 0.01 60 143 159 16 18 40 |
| 150 170 20 |
| 0.02 152 168 16 22 53 |
| 149 177 28 |
| 0.01 90 145 162 17 17.5 42 |
| 127 145 18 |
| 0.02 141 168 27 27 42 |
| 144 171 27 |
| 0.01 120 135 156 21 19 37 |
| 113 130 17 |
| 0.02 134 154 20 20.5 56 |
| 126 147 21 |
| 0.01 1B0 137 159 22 18 40 |
| 144 158 14 |
| 0.02 144 180 36 29.5 37 |
| 142 165 23 |
| 0.01 240 149 169 20 19 37 |
| 137 155 18 |
| 0.02 149 169 20 18 61 |
| 146 162 16 |
| 0.01 300 151 171 20 17.5 42 |
| 144 159 15 |
| 0.02 158 182 24 24 48 |
| 139 163 24 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 440, 490 grams |
| CONTROL 0.01 0 149 177 28 28 |
| 149 177 28 |
| 0.02 155 196 41 41 |
| 155 196 41 |
| Ex. No. 121 |
| 10 i.v. |
| 0.01 30 154 175 21 21 25 |
| 154 175 21 |
| 0.02 154 190 36 36 12 |
| 154 190 36 |
| Ex. No. 121 |
| 20 i.v. |
| 0.0i 60 155 168 13 13 54 |
| 155 168 13 |
| 0.02 156 175 19 19 54 |
| 156 175 19 |
| 0.01 90 159 172 13 13 54 |
| 159 172 13 |
| 0.02 157 179 22 22 46 |
| 157 179 22 |
| 0.01 120 159 164 5 5 82 |
| 159 164 5 |
| 0.02 154 173 19 19 54 |
| 154 173 19 |
| 0.01 180 151 169 18 18 36 |
| 151 169 18 |
| 0.02 159 173 14 14 66 |
| 159 173 14 |
| 0.01 240 149 169 20 20 29 |
| 149 169 20 |
| 0.02 151 172 21 21 49. |
| 151 172 21 |
| 0.01 300 151 168 17 17 39 |
| 151 168 17 |
| 0.02 152 177 25 25 39 |
| 152 177 25 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 585, 410 grams |
| CONTROL 0.01 0 140 162 22 17.5 |
| 14:7 160 13 |
| 0.02 138 169 31 33 |
| 141 176 35 |
| Ex. No. 123 |
| 10 i.v. |
| 0.0i 30 139 151 12 17.5 0 |
| 154 177 23 |
| 0.02 139 177 38 20 39 |
| 154 156 2 |
| 20 i.v. |
| 0.01 60 137 i40 3 6 66 |
| 147 156 9 |
| 0.02 132 139 7 6 82 |
| 146 151 5 |
| 0.01 90 137 141 4 6 66 |
| 149 157 |
| 0.02 135 138 3 7 79 |
| 149 160 11 |
| 0.01 120 136 139 3 5 71 |
| 147 154 7 |
| 0.02 138 141 3 4.5 86 |
| 150 156 6 |
| 0.01 180 138 141 3 4 77 |
| 150 155 5 |
| 0.02 138 142 4 6.5 80 |
| 148 157 9 |
| 0.01 240 137 145 8 8 54 |
| 146 154 8 |
| 0.02 140 146 6 6.5 80 |
| 148 155 7 |
| 0.01 300 138 144 6 8 54 |
| 144 154 10 |
| 0.02 140 148 8 8 76 |
| 146 154 8 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 500, 480 grams |
| CONTROL 0.01 0 150 172 22 32.5 |
| 140 183 43 |
| 0.02 147 187 40 35.5 |
| 162 193 31 |
| Ex. No.124 |
| 10 i.v. |
| 0.01 30 150 187 37 38.5 -1B |
| 153 193 40 |
| 0.02 148 187 39 43.5 -23 |
| 146 194 48 |
| 20 i.v. |
| 0.01 60 151 155 4 4 88 |
| 148 152 4 |
| 0.02 150 173 23 17.5 51 |
| 147 159 12 |
| 0.01 90 143 159 16 12.5 62 |
| 147 156 9 |
| 0.02 148 163 15 18 49 |
| 144 165 21 |
| 0.01 120 144 157 13 12.5 62 |
| 147 159 12 |
| 0.02 151 167 16 9.5 45 |
| 149 172 23 |
| 0.01 180 131 152 21 21 35 |
| 125 146 21 |
| 0.02 143 165 22 21.5 39 |
| 143 164 21 |
| 0.01 240 144 163 19 18 45 |
| 141 158 17 |
| 0.02 147 168 21 24.5 31 |
| 148 176 28 |
| 0.01 300 141 160 19 20 38 |
| 138 159 21 |
| 0.02 139 176 37 33.5 6 |
| 142 172 30 |
| SPONTANEOUSLY HYPERTENSIVE RATS n = 2 Body weight(s): 600, 550 |
| __________________________________________________________________________ |
| grams |
| TABLE IV |
| ______________________________________ |
| VASOPRESSIN (VAS) VASOPRESSOR RESPONSE |
| Ex. No. Dose mg/kg MAX % Inhibition |
| Time (Min) |
| ______________________________________ |
| 2 10 p.o. 65 180 |
| 136 10 i.v. 81 30 |
| 137 10 i.v. 52 30 |
| 138 10 i.v. 88 60 |
| 167 10 i.v. 70 180 |
| 251 30 i.v. 41 60 |
| 252 30 i.v. 75 120 |
| 253 30 i.v. 57 180 |
| 254 30 i.v. 74 60 |
| 255 10 i.v. 65 60 |
| 257 10 i.v. 71 60 |
| 258 30 i.v. 61 60 |
| 259 30 i.v. 29 90 |
| 260 30 i.v. 79 120 |
| 261 10 i.v. 74 60 |
| 263 10 i.v. 55 90 |
| 266 30 i.v. 21 120 |
| 267 10 i.v. 74 120 |
| 268 10 i.v. 85 90 |
| 379 10 i.v. 75 300 |
| 416 30 i.v. 44 90 |
| 448 30 i.v. 60 90 |
| 449 10 i.v. 45 90 |
| 455 30 i.v. 46 120 |
| 457 10 i.v. 61 300 |
| 458 10 i.v. 77 180 |
| ______________________________________ |
(a) Membrane Preparation
Female Sprague-Dawley rats weighing approximately 200-250 g are injected intramuscularly (i.m.) with 0.3 mg/kg of body weight of diethylstilbestrol (DES). The rats are sacrificed 18 hours later under pentobarbital anesthesia. The uteri are dissected out, cleaned of fat and connective tissues and rinsed in 50 ml of normal saline. The tissue pooled from six rats is homogenized in 50 ml of 0.01 mM Tris.HCl, containing 0.5 mM dithiothreitol and 1.0 mM EDTA, adjusted to pH 7.4, using a polytron at setting 6 with three passes of 10 sec each. The homogenate is passed through two (2) layers of cheesecloth and the filtrate centrifuged at 1000×g for 10 min. The clear supernatant is removed and recentrifuged at 165,000×g for 30 min. The resulting pellet containing the oxytocin receptors is resuspended in 50.0 mM Tris.HCl containing 5.0 mM MgCl2 at pH 7.4, to give a protein concentration of 2.5 mg/ml of tissue suspension. This preparation is used in subsequent binding assays with [3 H]Oxytocin.
(b) Radioligand Binding
Binding of 3,5-[3 H]Oxytocin ([3 H]OT) to its receptors is done in microtiter plates using [3 H]OT, at various concentrations, in an assay buffer of 50.0 mM Tris.HCl, pH 7.4 and containing 5.0 mM MgCl2, and a mixture of protease inhibitors: BSA, 0.1 mg; aprotinin, 1.0 mg; 1,10-phenanthroline, 2.0 mg; trypsin, 10.0 mg; and PMSF, 0.3 mg per 100 ml of buffer solution. Non-specific binding is determined in the presence of 1.0 uM unlabeled OT. The binding reaction is terminated after 60 min., at 22°C, by rapid filtration through glass fiber filters using a Brandel® cell harvester (Biomedical Research and Development Laboratories, Inc., Gaithersburg, Md.). Competition experiments are conducted at equilibrium using 1.0 nM [3 H]OT and varying the concentration of the displacing agents. The concentrations of agent displacing 50% of [3 H]OT at its sites (IC50) are calculated by a computer assisted LUNDON-2 program (LUNDON SOFTWARE INC., Ohio, USA).
The results of this assay on representative examples are shown in Table V.
| TABLE V |
| ______________________________________ |
| Oxytocin |
| Ex. No. Dose (μM) |
| % Inhibition at 10 μM |
| IC50 |
| ______________________________________ |
| 2 10 73 2.9 |
| 5 10 90 2 |
| 11 10 71 4.4 |
| 249 10 38 |
| 250 10 15 |
| 251 10 77 |
| 253 10 77 3.1 |
| 254 10 95 1.9 |
| 255 10 99 0.46 |
| 256 10 47 6.5 |
| 257 10 97 0.189 |
| 258 10 18 |
| 259 10 7 |
| 260 10 44 |
| 261 10 97 0.21 |
| 262 10 55 6.2 |
| 263 10 94 0.26 |
| 264 1 34 |
| 266 10 15 |
| 267 10 89 1.1 |
| 268 10 89 2.4 |
| 345 10 2 |
| 379 10 96 0.81 |
| 416 10 19 |
| 448 10 39 |
| 450 10 33 |
| 451 1 0 |
| ______________________________________ |
| Oxytocin |
| Ex. No. Dose (μM) |
| % Inhibition at 10 μM |
| IC50 μM |
| ______________________________________ |
| 452 1 22 |
| 453 5 53 3.3 |
| 454 10 78 2.5 |
| 455 10 88 1.1 |
| 534 10 86 0.94 |
| 581 10 95 0.475 |
| 582 0.275 |
| 583 10 95 0.33 |
| 584 10 100 0.17 |
| 585 10 99 0.25 |
| ______________________________________ |
The compounds of the present invention can be used in the form of salts derived from pharmaceutically or physiologically acceptable acids or bases. These salts include, but are not limited to, the following: salts with inorganic acids such as hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid and, as the case may be, such organic acids as acetic acid, oxalic acid, succinic acid, and maleic acid. Other salts include salts with alkali metals or alkaline earth metals, such as sodium, potassium, calcium or magnesium or with organic bases. The compounds can also be used in the form of esters, carbamates and other conventional "pro-drug" forms, which, when administered in such form, convert to the active moiety in vivo. When the compounds are employed for the above utility, they may be combined with one or more pharmaceutically acceptable carriers, for example, solvents, diluents and the like, and may be administered orally in such forms as tablets, capsules, dispersible powders, granules, or suspensions containing, for example, from about 0.05 to 5% of suspending agent, syrups containing, for example, from about 10 to 50% of sugar, and elixirs containing, for example, from about 20 to 50% ethanol, and the like, or parenterally in the form of sterile injectable solution or suspension containing from about 0.05 to 5% suspending agent in an isotonic medium. Such pharmaceutical preparations may contain, for example, from about 0.05 up to about 90% of the active ingredient in combination with the carrier, more usually between about 5% and 60% by weight.
The effective dosage of active ingredient employed may vary depending on the particular compound employed, the mode of administration and the severity of the condition being treated. However, in general, satisfactory results are obtained when the compounds of the invention are administered at a daily dosage of from about 0.5 to about 500 mg/kg of animal body weight, preferably given in divided doses two to four times a day, or in a sustained release form. For most large mammals the total daily dosage is from about 1 to 100 mg, preferably from about 2 to 80 mg. Dosage forms suitable for internal use comprise from about 0.5 to 500 mg of the active compound in intimate admixture with a solid or liquid pharmaceutically acceptable carrier. This dosage regimen may be adjusted to provide the optimal therapeutic response. For example, several divided doses may be administered daily or the dose may be proportionally reduced as indicated by the exigencies of the therapeutic situation.
These active compounds may be administered orally as well as by intravenous, intramuscular, or subcutaneous routes. Solid carriers include starch, lactose, dicalcium phosphate, microcrystalline cellulose, sucrose and kaolin, while liquid carriers include sterile water, polyethylene glycols, non-ionic surfactants and edible oils such as corn, peanut and sesame oils, as are appropriate to the nature of the active ingredient and the particular form of administration desired. Adjuvants customarily employed in the preparation of pharmaceutical compositions may be advantageously included, such as flavoring agents, coloring agents, preserving agents, and antioxidants, for example, vitamin E, ascorbic acid, BHT and BHA.
The preferred pharmaceutical compositions from the standpoint of ease of preparation and administration are solid compositions, particularly tablets and hard-filled or liquid-filled capsules. Oral administration of the compounds is preferred.
These active compounds may also be administered parenterally or intraperitoneally. Solutions or suspensions of these active compounds as a free base or pharmacologically acceptable salt can be prepared in water suitably mixed with a surfactant such as hydroxypropylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols and mixtures thereof in oils. Under ordinary conditions of storage and use, these preparation contain a preservative to prevent the growth of microorganisms.
The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases, the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol(e.g., glycerol, propylene glycol and liquid polyethylene glycol), suitable mixtures thereof, and vegetable oils.
PAC 6,11-Dihydro-5H-dibenz[b,e]azepineA mixture of 48.52 g (0.20 mol) of 2-amino-benzophenone-2'-carboxylic acid and 500 ml of xylene is refluxed for 67 hours, cooled to room temperature and filtered. The solid is washed with xylene to give 43.3 g (97.8%) of 5H-dibenz[b,e]azepine-6,11-dione as light tan crystals, m.p. 245°-248°C To 4.46 g (0.020 mol) of the preceding compound in 25 ml of tetrahydrofuran is added 12 ml (0.12 mol) of a 10 molar solution of boron-dimethylsulfide in tetrahydrofuran. An additional 10 ml of tetrahydrofuran is added and the mixture is stirred overnight and then is refluxed (solids dissolve) for 4 hours. The solution is cooled and 15 ml of methanol added dropwise. The mixture is concentrated under vacuum, 50 ml of 2N sodium hydroxide is added and the mixture refluxed for 2 hours. The solid is filtered, washed with water, air dried and extracted with dichloromethane. The extract is dried (Na2 SO4) and the solvent removed to give 3.25 g (83%) of crystals, m.p. 117°-122°C
PAC 4-[(2-Methylbenzoyl)amino]benzoic acidA mixture of 43.42 g (0.26 mol) of ethyl 4-aminobenzoate and 40.8 g (0.26 mol) of 2-methylbenzoyl chloride in 150 ml of dichloromethane is cooled in an ice bath and 26.56 g (0.26 mol) of triethylamine is added dropwise. After the addition, the solution is stirred at room temperature overnight. The mixture is poured into water and the organic layer separated. The organic layer is washed with water, 1N HCl, 1M NaHCO3 and dried (Na2 SO4). The solvent is removed and the solid slurried with ethyl acetate and filtered to give 57 g of ethyl 4-[(2-methylbenzoyl)amino]benzoate as crystals, m.p. 110°-115°C
A mixture of 50.7 g (0.20 mol) of the preceding compound, 280 ml of ethanol and 55 ml of 10N NaOH is refluxed for 5 minutes. The mixture is cooled to room temperature, diluted with 200 ml of water and acidified with concentrated hydrochloric acid (pH 1-2). The mixture is filtered and the solid washed with water and dried to give 51 g of product as white crystals, m.p. 270°-275°C
PAC 4-[(2-Methylbenzoyl)amino]benzoyl chlorideA mixture of 10.3 g of 4-[(2-methylbenzoyl)amino]benzoic acid and 32 ml of thionyl chloride is refluxed for 1.5 hours. The solution is concentrated under vacuum. Toluene is added and the solvent removed under vacuum. Toluene is added and the mixture chilled and filtered to give a yellow solid, m.p. 135°-141°C
PAC 4[(2,6-Dimethoxybenzoyl)amino]benzoic acidA mixture of 2 g (10 mmol) of 2,6-dimethoxybenzoyl chloride, 1.65 g (10 mmol) of ethyl 4-aminobenzoate, 1.11 g of triethylamine and 61 mg of 4-dimethylaminopyridine in 10 ml of dichloromethane is refluxed for 20 hours. The mixture is diluted with water and the organic layer separate. The organic layer is washed with water, 1N HCl, 1N Na2 CO3, brine and dried (Na2 SO4). The solvent is removed to give a solid which is crystallized from ethyl acetate to give 1.22 g of ethyl 4-[(2,6-dimethoxybenzoyl)amino]benzoate as crystals, m.p. 183°-185°C
A mixture of 3.88 g (11.79 mmol) of the preceding compound, 17.3 ml of 2N NaOH and 20 ml of methanol is stirred at room temperature overnight. Methanol (30 ml) and water (10 ml) are added and the solution refluxed for 1/2 hour. The solvents are removed under vacuum and the residual solid triturated with ether and the ether decanted. The solid is dissolved in 30 ml of water and acidified with 2N HCl (pH 3). The mixture is filtered, the solid washed with water and dried at 60°C under vacuum to give 3.0 g of solid, m.p. 236°-240°C
PAC 4-[(4-Pyridinylcarbonyl)amino]benzoic acidTo a cooled mixture of 1.78 g (0.01 mol) of isoniconinoyl chloride hydrochloride in 5 ml of dichloromethane is added 2.52 g (0.025 mol) of triethylamine. To the solution is added a solution of 1.65 g of ethyl 4-aminobenzoate in 5 ml of dichloromethane. After stirring at room temperature overnight, 50 mg of 4-dimethylaminopyridine is added and the mixture is refluxed for 24 hours. The mixture is poured into water and filtered to give 3.4 g of brown solid. A 0.50 g sample is triturated with ethyl acetate to give 0.37 g of ethyl 4-[(4-pyridinylcarbonyl)amino]benzoate as yellow crystals, m.p. 143°-145°C
Anal. Calc'd for C15 H14 N2 O3 : C,66.7; H,5.2; N,10.4; Found: C,66.4; H,5.1; N,10.3.
A solution of 8.15 g (30 mmol) of the preceding compound and 22 ml of 2N NaOH in 60 ml of methanol is heated on a steam bath for 1 hour. The mixture is cooled and filtered to the solid in water is added 2N citric acid. Stirring and filtering gives 4.24 g of crystals, m.p. 362°-365°C
Anal. Calc'd for C13 H10 N2 O3 1/2 H2 O C,62.1; H,4.4; N,11.1; Found: 62.6; H,4.3; N,11∅
PAC 4-[(3-Pyridinylcarbonyl)amino]benzoic acidA mixture of 1.83 g (0.01 mol) of nicotinoyl chloride hydrochloride (97%), 1.65 g of (0.01 mol) of ethyl 4-aminobenzoate, 2.22 g (0.022 mol) of triethylamine and 61 mg of 4-dimethylaminopyridine in 33 ml of dichloromethane is refluxed 24 hours. The solution is washed with water, 2N citric acid and NaHCO3 solution. The solvent is removed and the residue triturated with methanol to give 2.3 g of ethyl 4-[(3-pyridinylcarbonyl)amino]benzoate as yellow crystals, m.p. 125°-127 °C
A mixture of 12.0 g (0.044 mol) of ethyl 4-[(3-pyridinylcarbonyl)amino]benzoic acid, 65 ml of 2N sodium hydroxide and 120 ml of methanol is refluxed for 0.75 hour. The solvent is removed and the residue extracted with diethyl ether. The residue is diluted with water and solid citric acid is added until the pH is 4-5. The mixture is filtered and the solid washed with water and air dried to give crystals, m.p. 307°-310°C
PAC 5,6-Dihydro-5-(4-nitrobenzoyl)phenanthridineTo a suspension of 7.5 g of 5,6-dihydrophenanthridine in 40 ml of warm pyridine under nitrogen is added 3.6 g of 4-nitrobenzoyl chloride. The mixture is stirred overnight, filtered and the solid washed twice with 5 ml of pyridine. To the filtrate is added 250 ml of 2N HCl and the mixture stirred and then filtered to give 6.6 g of solid. This solid is heated with 25 ml of ethyl acetate and filtered. The filtrate is diluted with 25 ml of hexane and filtered. The filtrate is chromatographed HPLC on a Waters-Prep 500 instrument with two silica gel columns and hexane-ethyl acetate (4:1) as solvent. Cuts containing product are combined to give 2.3 g of yellow crystals, m.p. 153° to 154°C
Anal. Calc'd for C20 H14 N2 O3 : C,72.7; H,4.3; N,8.5; Found: 72.0; H,4.3; N,8.3.
PAC 5-(4-Aminobenzoyl)-5.6-dihydrophenanthridineA solution of 2.15 g of 5,6-dihydro-5-(4-nitrobenzoyl)phenanthridine in 50 ml of ethyl acetate and 0.5 g of 10% palladium-on-carbon is hydrogenated in a Parr apparatus under an atmosphere of hydrogen for three hours. The mixture is filtered through diatomaceous earth and the solvent removed to give 1.7 g of the product as a yellow foam.
PAC 6,11-Dihydro-5-(4-nitrobenzoyl)-5H-dibenz [b,e]-azepineA mixture of 2.34 g (12 mmol) of 6,11-dihydro-5H-dibenz[b,e]azepine, 2.23 g (12 mmol) of 4-nitrobenzoyl chloride, 1.94 g (15 mmol) of diisopropylethylamine and 70.5 mg of 4-(dimethylamino)pyridine in 25 ml of dichloromethane is stirred at room temperature for 2 hours, refluxed for 3 hours and allowed to stand at room temperature for 2 days. The mixture is washed with water, 1N sodium bicarbonate, water, 1N HCl, brine and dried (Na2 SO4). The solvent is removed to give 4.0 g of solid. Trituration with ethyl acetate and filtering gives 2.85 g of off-white crystals, m.p. 185°-188°C
Anal. Calc'd for C21 H16 N2 O3 0.5 H2 O: C,71.4; H,4.7; N,7.9. Found: C,71.5; H,4.5; N,7.9.
PAC 5-(4-Aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepineTo a solution of 4.5 g of 6,11-dihydro-5-(4-nitrobenzoyl)-5H-dibenz[b,e]azepine in 230 ml of glacial acetic acid is added 0.58 g of 10% palladium-on-carbon and the mixture under hydrogen (38 psi) shaken in a Parr hydrogenator for 6.5 hours. The mixture is filtered through diatomaceous earth and the filtrate concentrated to give 4.0 g of solid. The solid is extracted with 100 ml of dichloromethane and the extract washed with water, dried (Na2 SO4). The solvent is removed to give 4.0 g of yellow crystals, m.p. 168°-175°C A sample chromatographed on a thick layer silica gel plate with hexane-ethyl acetate (8:7) as solvent gives yellow crystals, m.p. 173°-175°C
Anal. Calc'd for C21 H18 N2 O: C,80.2; H,5.8; N,8.9. Found: C,79.2; H,6.0; N,8.8.
PAC 2-Chloro-5H-dibenz [b,e]azepine-6,11-dioneChlorine gas is bubbled into a mixture (partial suspension) of 1.0 g (450 mmol) of 5H-dibenz[b,e]azepine-6,11-dione in 50 ml of glacial acetic acid. The temperature of the mixture rises to 38°C On standing, as the temperature of the solutions decreases, a white solid precipitates. The mixture is filtered to give 0.40 g of solid (mixture of starting material and product in ratio of 1:8). The filtrate on standing gives 0.10 g of product as crystals, m.p. 289°-293°C
PAC 10,11-Dihydro-N,N-dimethyldibenz[b,f][1,4]oxazepine-2-sulfonamideTo 5.88 g of 10,11-dihydro-N,N-dimethyl-11-oxodibenz[b,f][1,4]oxazepine-2-sulfonamide in 5 ml of tetrahydrofuran is added 20 ml of a molar solution of borane-dimethylsulfide in tetrahydrofuran. The mixture is stirred overnight and then refluxed for 2 hours. The mixture is chilled, diluted with 10 ml of methanol and then concentrated, methanol added again and the mixture concentrated. To the mixture is added 20 ml of 2N NaOH and the mixture refluxed for 2 hours. The mixture is extracted with dichloromethane, the extract dried (MgSO4) and filtered. The filtrate is passed through a thin pad of hydrous magnesium silicate and the pad washed with dichloromethane. The filtrate is concentrated to give 4.8 g of crystals, m.p. 99°-102°C Recrystallization from diisopropylether-dichloromethane gives 3.96 g of crystals, m.p. 109°-110°C Mass Spectrum (FAB) 305(M+H). Anal. Calc'd for C15 H16 N2 O3 S: C,59.2; H,5.3; N,9.2; S,10.6. Found: C,57.6; H,5.2; N,8.9; S,10.1.
PAC 10,11-Dihydro-N,N-dimethyl-10-(4-nitrobenzoyl)-dibenz[b,f][1,4]oxazepine-2- sulfonamideA mixture of 0.9 g of 10,11-dihydro-N,N-di-methyldibenz[b,f][1.4]oxazepine-2-sulfonamide and 0.55 g of 4-nitrobenzoyl chloride in 3 ml of pyridine is stirred for 7 hours. To the mixture is added 20 ml of 2N hydrochloric acid and the mixture is extracted with ethyl acetate. The extract is washed with 5 ml of 2N HCl and three times with 5 ml of 2N Na2 Co3. The extract is dried (MgSO4) and filtered through a thin pad of hydrous magnesium silicate. The pad is washed with ethyl acetate and the filtrate concentrated to give 1.1 g of a yellow solid. Crystallization from dichloromethane-diisopropylether gives 0.62 g of crystals, m.p. 177°-178°C
PAC 2-Chloro-5,6-dihydrophenanthridineTo a hot (70°C) solution of 2.62 g (17 mmol) of 6(5H)-phenanthridinone in 120 ml of acetic acid is added chlorine gas for 10 minutes. The solution is allowed to cool to room temperature and the mixture filtered. The crystals are filtered to give 1.35 g of crystals, m.p. 310°-318°C
To the preceding compound (1.57 g) in 25 ml of tetrahydrofuran is added 12 ml of a 10 molar solution of boron-dimethylsulfide in tetrahydrofuran. The mixture is refluxed for 18 hours, cooled and 15 ml of methanol is added. The mixture is concentrated under vacuum and 50 ml of 2N sodium hydroxide added. The mixture is refluxed for 2 hours and the solid filtered off and washed with water and air dried to give the product as a solid.
PAC 9-Chloro-5H-dibenz[b,e]azepin-6,11-dioneA mixture of 11.15 g of 5H-dibenz[b,e]azepin-6,11-dione and 600 ml of glacial acetic acid is heated on a steam bath until the solid dissolves. To the solution (70°C) is added chlorine gas. Chlorine is bubbled through the solution until a precipitate begins to form. The mixture is allowed to cool to room temperature and is filtered to give 7.3 g of product, m.p. 290°C to 295°C
PAC 9-Chloro-6,11-dihydro-5H-dibenz[b,e]azepineTo a mixture of 7.28 g of 9-chloro-5H-dibenz-[b,e]azepin-6,11-dione in 25 ml of tetrahydrofuran under argon is added 8.5 ml of 10 molar boron-dimethylsulfide in tetrahydrofuran. The mixture is stirred 18 hours at room temperature, 30 ml of tetrahydrofuran added and the mixture refluxed for 3 hours (solids dissolved). The solution is cooled to room temperature and 25 ml of methanol added dropwise. The volatiles are removed under vacuum. To the residue is added 100 ml of 2N NaOH. The mixture is refluxed overnight and filtered. The solid is extracted with dichloromethane and the extract is washed with 2N citric acid, water and dried (Na2 SO4). The solvent is removed to give 4.2 g of solid which is triturated with ethyl acetate-hexane (1:2) to give crystals, m.p. 137°C to 141°C
PAC 10,11-Dihydrodibenz[b,f][1,4]thiazepineTo a mixture of 3.3 g of 10,11-dihydro-11-oxodibenz[b,f][1,4]thiazepine in 25 ml of tetrahydrofuran is added 4.0 ml of 10 molar borane-dimethylsulfide in tetrahydrofuran. The mixture is stirred at room temperature for 18 hours, 50 ml of anhydrous methanol added and the solvent removed. An additional 30 ml of methanol is added and the solvent removed to give white crystals. A sample is purified by chromatography on silica gel with hexane-chloroform-ethyl acetate (2:1:1) as solvent to give white crystals, m.p. 145°-148°C
The following compounds are prepared as described in Reference Example 17.
PAC 4-Methyl-10,11-dihydrodibenz[b,f][1,4]thiazepine PAC 4-Chloro-10,11-dihydrodibenz[b,f][1,4]thiazepine PAC 2-Methyl-10,11-dihydrodibenz[b,f][1,4thiazepine PAC 2-Chloro-10,11-dihydrodibenz[b,f][1,4thiazepine PAC 2-Methoxy-10,11-dihydrodibenz[b,f][1,4thiazepine PAC 8-Chloro-10,11-dihydrodibenz[b,f][1,4thiazepine PAC 4,8-Dichloro-10,11-dihydrodibenz[b,f][1,4thiazepine PAC 8-Chloro-4-methyl-10,11-dihydrodibenz[b,f][1,4]-thiazepine PAC 8-Methoxy-10,11-dihydrodibenz[b,f][1,4]thiazepine PAC 7-Chloro-4-methyl-10,11-dihydrodibenz[b,f][1,4]-thiazepineThe following compounds are prepared as described in Reference Example 12.
PAC 2-Chloro-10,11-dihydrodibenz[b,f][1,4]-oxazepine PAC 2-Methyl-10,11-dihydrodibenz[b,f][1,4]-oxazepine PAC 4-chloro-10,11-dihydrodibenz[b,f][1,4]-oxazepine PAC 3-Methyl-10,11-dihydrodibenz[b,f][1,4]-oxazepine PAC 7-Chloro-10,11-dihydrodibenz[b,f][1,4]-oxazepine PAC 8-Chloro-10,11-dihydrodibenz[b,f][1,4]-oxazepine PAC 2,4-Dichloro-10,11-dihydrodibenz[b,f][1,4]-oxazepine PAC 4,8-Dichloro-10,11-dihydrodibenz[b,f][1,4]-oxazepine PAC 4-Chloro-8-methyl-10,11-dihydrodibenz[b,f][1,4]-oxazepine PAC 4-Methyl-7-chloro-10,11-dihydrodibenz[b,f][1,4]-oxazepine PAC 1-Chloro-4-methyl-10,11-dihydrodibenz[b,f][1,4]-oxazepine PAC 2-Fluoro-10,11-dihydrodibenz[b,f][1,4-oxazepine PAC N-(2-Iodophenyl)-2-iodophenylacetamideA solution of 13.32 g (0.05 mol) of 2-iodophenylacetic acid in 75 ml thionyl chloride is refluxed for 2 hours, and the volatiles removed under vacuum. Toluene is added (3 times) and the solvent removed under vacuum after each addition to give 2-iodophenylacetyl chloride as a gum. To the preceding compound (0.05 mol) in a mixture of 100 ml of toluene-dichloromethane (1:1) is added 11 g (0.05 mol) of 2-iodoaniline and (0.10 mol) of diisopropylethylamine. The mixture is stirred at room temperature overnight and the solvent removed. The residue is dissolved in dichloromethane and the solution washed with 1N HCl, saturated sodium bicarbonate, brine and dried (Na2 SO4). The solvent is removed and the residue recrystallized from methanol-ether to give 16.0 g of light brown crystals, m.p. 160°-163°C
PAC 2-Iodo-N-(2-iodophenyl)benzeneethanamineTo a suspension of 1.39 g (3 mmol) of 2-iodo-N-(2-iodophenyl)benzeneacetamide in 30 ml of tetrahydrofuran-dichloromethane (1:1) is added 3.75 ml of 2.0 molar borane-dimethylsulfide in tetrahydrofuran. The solution is stirred 1 hr at room temperature and then refluxed for 16 hours. The mixture is cooled and water slowly added dropwise until gas evolution ceases. The volatiles are removed under vacuum and the aqueous residue made alkaline with 2N sodium hydroxide. The mixture is extracted with ether (50 ml) and the extract is washed with brine and dried (Na2 SO4). The solution is filtered through a thin pad hydrous magnesium silicate and the filter pad is washed with ether and the filtrate evaporated. The residual solid is washed with isooctane to give 1.20 g of white solid. Recrystallization from diethylether/hexane gives white crystals.
PAC N-(4-Nitrobenzoyl-N-(2-iodophenyl)-2-iodobenzeneethyl-amineTo a solution of 0.90 g of 2-iodo-N-(2-iodo-phenyl)benzeneethanamine in 4 ml of tetrahydrofuran is added 0.41 g of triethylamine, and 0.57 g of 4-nitro-benzoyl chloride. The mixture is stirred at room temperature for 2 hours and the solvent removed under vacuum. The residue is dissolved in ethyl acetate-di-chloromethane (5:1) and the solution washed with 1N HCl, saturated NaHCO3, brine and dried (Na2 SO4). The solution is filtered through a thin pad of hydrous magnesium silicate. The filtrate is evaporated and the residual solid triturated with diethyl ether and hexane to give 1.10 g of product as a white solid.
PAC 5-(4-Nitrobenzoyl)-6,7-dihydro-5H-dibenz[b,d]azepineTo a solution of 0.90 g of N-(4-nitrobenzoyl)-N-(2-iodophenyl)-2-iodobenzeneethylamine in 10 ml of N,N-dimethylpropyleneurea (DMPU) is added 1.91 g of "activated" copper bronze. The mixture is stirred and heated at 195° for 2 days, cooled and slowly dropped into 100 ml of 0.5N HCl with stirring. The precipitate is filtered, washed with H2 O and air dried (1.0 g of solid). The solid is extracted with ethyl acetate to give 0.50 g of solid. Chromatography on thick layer silica gel plates with ethyl acetate-hexane (1:2) as solvent gives 0.15 g of yellow solid.
PAC 5-(4-Aminobenzoyl)-6,7-dihydro-5H-dibenz[b,d]azepineA solution of 0.15 g of 5-(4-nitrobenzoyl)-6,7-dihydro-5H-dibenz[b,d]azepine in 20 μl of ethanolethyl acetate and 10 mg of 10% palladium-on-carbon is hydrogenated under 35 pounds per square inch of hydrogen for 8 hr. The mixture is filtered through diatomaceous earth and the filtrate evaporated to give 0.13 g of product as a light yellow solid.
PAC 5-(4-Nitro-3-methylbenzoyl)-6,11-dihydro-5H-dibenz-[b,e]azepineA mixture of 1.17 g of 6,11-dihydro-5H-di-benz[b,e]azepine, 1.20 g of 3-methyl-4-nitrobenzoyl chloride, 0.80 ml of diisopropylethylamine in 25 ml of dichloromethane is stirred 18 hours at room temperature. The mixture is washed with water and dried (Na2 SO4). The solution is filtered through a thin pad of hydrous magnesium silicate. The filtrate is concentrated and diluted while hot with hexane to give 1.40 g of crystals. Recrystallization from dichloromethane-hexane gives 1.26 g of crystals, m.p. 179°-180°C
PAC 5-(4-Amino-3-methylbenzoyl)-6,11-dihydro-5H-dibenz-[b,e]azepineA mixture of 1.11 g of 5-(4-nitro-3-methyl-benzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 50 ml of ethanol, 0.30 g of anhydrous hydrazine and 0.27 g palladium-on-carbon is refluxed 1 hour, and then filtered through diatomaceous earth. The filtrate is evaporated and the residue recrystallized from dichloromethane-hexane to give 0.80 g of crystals, m.p. 203°-204°C
PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-benzoic acidA mixture of 0.975 g of 6,11-dihydro-5H-dibenz[b,e]azepine and 0.20 g of NaH (60% in oil) in 20 ml of tetrahydrofuran is stirred at room temperature for 0.5 hr. Then 1.1 g of mono-methyl terephthalyl chloride (prepared from mono-methyl terephthalate and thionyl chloride) is added and the mixture refluxed 18 hours. The mixture is cooled, poured into ice water and filtered. The solid is triturated with dichloromethane-hexane to give 1.0 g of crystals, m.p. 182°-185°C The preceding compound, methyl 4-[(6,11-dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]benzoate (2.11 g), 20 ml of 2N NaOH and 20 ml of methanol is stirred overnight and then heated on a steam bath for 1 hr. The solvent is removed to give a solid. The solid is extracted with ether (discarded). The solid is dissolved in water and the solution acidified with citric acid to give a solid. The solid is filtered and washed with water to give 1.84 g of crystals, m.p. 220°-225°C
PAC 2-Methylfurane-3-carbonyl chlorideA mixture of 4.0 g of methyl-2-methylfurane-3-carboxylate, 30 ml of 2N NaOH and 15 ml methanol is refluxed for 1.5 hours. The solvent is removed under vacuum to give a solid. The solid is extracted with dichloromethane (discarded). The solid is dissolved in water and the solution acidified with 2N citric acid to give a solid. The solid is washed with water and dried to give crystals 1.05 g of crystals of 2-methylfuran-3-carboxylic acid. The preceding compound (0.95 g) and 3 ml of thionyl chloride is refluxed for 1 hr. The solvent is removed, toluene added (20 ml, three times) and the solvent removed to give the product as an oil.
PAC 2-Chloro-5-(4-nitrobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepineA mixture of 2.05 g of 2-chloro-6,11-dihydro-5H-dibenz[b,e]azepine, 2.15 g of 4-nitrobenzoyl chloride, 1.50 g of N,N-diisopropylethylamine, 54 mg of 4-(dimethylamino)pyridine in 15 ml of dichloromethane is refluxed for 18 hours. The mixture is cooled and washed with H2 O, 1N HCl, 1M NaHCO3, brine and dried (Na2 SO4). The solution is filtered through a thin pad of hydrous magnesium silicate and the pad washed with dichloromethane. The filtrate is concentrated and the residue recrystallized from dichloromethane-hexane to give 2.33 g of crystals, m.p. 198°-201°C
PAC 2-Chloro-5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepineA solution of 2.1 g of 2-chloro-5-(4-nitro-benzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine in 400 ml of ethyl acetate-ethanol (1:1) and 0.25 g of palladium-on-carbon is hydrogenated in a Parr hydrogenator under 38 pounds per square inch of hydrogen. The mixture is filtered through diatomaceous earth and the filtrate concentrated to dryness. The solid (1.94 g) is dissolved in dichloromethane and the solution filtered through a thin pad of hydrous magnesium silicate. The filter pad is washed with dichloromethane and the filtrate concentrated. The residue is crystallized from dichloromethane-hexane to give 1.43 g of crystals, m.p. 211°-214°C
PAC 1,2,3,4-Tetrahydro-1-(4-nitrobenzoyl)-5H-1-benzazepineTo a solution of 5.0 g of 1,2,3,4-tetrahydro-5H-1-benzazepine and 5.91 ml of triethylamine in 50 ml of dichloromethane, chilled in an ice bath, is added, dropwise, 6.32 g of 4-nitrobenzoyl chloride in 75 ml of dichloromethane. The mixture is stirred at 0°C for 1.5 hr and then at room temperature for 18 hours. The mixture is washed with H2 O, 1M HCl, 1M NaOH and dried (Na2 SO4). The solvent is removed and the residue re-crystallized from ethanol with the aid of activated carbon to give 7.75 g of light yellow crystals, m.p. 148.5°-150.5°C
PAC 1,2,3,4-Tetrahydro-1-(4-nitrobenzoyl)-5H-1-benzapin-5-oneA sample (3.0 g) of 1,2,3,4-tetrahydro-1-(4-nitrobenzoyl)-5H-1-benzazepine is dissolved in 200 ml of tert-butanol (with heat). To the solution is added 8 ml of H2 O and 6.72 g of anhydrous MgSO4 and then a solution of 3.36 g of KMnO4 in 100 ml of H2 O is added and the mixture heated at 65°C for 20 hours. The mixture is filtered through diatomaceous earth and the filter pad washed with tert-butanol. The combined filtrate is concentrated (t-butanol removed) under vacuum. The solid is filtered and washed well with water to give (after drying) 2.7 g of crystals. Chromatography on silica gel with hexane-ethyl acetate (2:1) gives 1.72 g of recovered starting material and 0.81 g of product as crystals, m.p. 135°-137°C
PAC 3,4-Dihydro-1H-1-benzazepine-2,5-dioneTo a solution of 225 ml of glacial acid and 8.5 ml of concentrated sulfuric acid is added 49.54 g (0.30 mol) of 2'-nitroacetophenone and 47.02 g (0.50 mol) of glyoxylic acid (hydrated). The mixture is heated at 100°C 16 hours. The mixture is cooled and poured over crushed ice. After the ice melts, the mixture is filtered and the solid washed with cold water. The solid is dried and recrystallized from dichloromethane-hexane to give 20.1 g of 3-(2-nitrobenzoyl)acrylic acid as white crystals, m.p. 153°-158°C A solution of the preceding compound (9.0 g) in 80 ml of ethanol and 1.6 g of palladium-on-carbon is hydrogenated in a Parr hydrogenator under 30 pounds per square inch of hydrogen for 20 hours. The mixture is filtered through diatomaceous earth and the solvent is removed. The residue (7.0 g) is chromatographed on silica gel with hexane-ethyl acetate (1:1) as solvent to give 4.0 g of 3-(2-aminobenzoyl)acrylic acid as an orange solid, m.p. 103°-107°C A 0.50 g sample of the preceding compound, 0.36 ml of triethylamine and 0.43 ml of diethoxyphosphinyl cyanide in 20 ml of dichloromethane is stirred at room temperature for 5 days. The solvent is removed, ethyl acetate is added and the mixture washed with water, 2N citric acid, 1M NaHCO3, brine and dried (Na2 SO4). The solvent is removed and the residue purified by chromatography over silica gel with ethyl acetate-hexane (1:1) as solvent to give 0.190 g of light brown crystals, m.p. 168°-170°C
PAC 4-[(Dimethylamino)methylene]-1,2,3,4-tetrahydro-1-(4-nitrobenzoyl)-5H-1-ben zazepin-5-oneA mixture of 0.780 g of 1,2,3,4-tetrahydro-1-(4-nitrobenzoyl)-5H-1-benzazepin-5-one and 10 ml of tert-butoxy bis(dimethylamino)methane (Bredereck's Reagent) is heated on a steam bath while stirring for 1.5 hours (solid dissolved). Cooling gives a solid and the mixture is diluted with ethyl acetate and filtered. The solid is dissolved in dichloromethane-ethyl acetate (7:3) and the solution filtered through a thin pad of hydrous magnesium silicate. The filter pad is washed with dichloromethane-ethyl acetate (7:3) and the combined filtrate evaporated to give 0.43 g of yellow crystals, Mass Spec (CI) MH+ =366, m.p. 180°-183°C
PAC 6,7-Dihydro-7-(4-nitrobenzoyl)-5H-pyrimido-[5,4-d][1]benzazepineTo a solution of 0.152 g (1.89 mmol) of formamidine hydrochloride in 10 ml of methanol under argon is added 0.102 g (1.89 mmol) of sodium methoxide. After stirring 5 min., a solution of 0.46 g (1.26 mmol) of 4-[(dimethylamino)methylene]-1,2,3,4-tetrahydro-1-(4-nitrobenzoyl)-5H-1-be nzazepine-5-one in 5 ml of methanol is added and the mixture stirred 18 hours. The solvent is removed, dichloromethane added, and the mixture filtered. The solid is washed with dichloromethane. The combined filtrate is concentrated to dryness to give 0.47 g of tan foam. The preceding solid is purified by chromatography on silica gel. The compound is dissolved in ethyl acetate-dichloromethane and applied to the column. The column is then eluted with ethyl acetate to give 0.25 g of product as pale yellow crystals.
PAC 4-[(Dimethylamino)methylene]-3,4-dihydro-1H-1-benzazepine-2,5-dioneA mixture of 0.250 g (1.43 mmol) of 3,4-dihydro-1H-1-benzazepine-2,5-dione and 5.5 ml (4.93 g, 41.5 mmol) of N,N-dimethylformamide, dimethylacetal is heated at 90°C for 1.5 hour. The mixture is cooled, diluted with diethylether and filtered. The solid is washed well with diethylether and dried to give 0.26 g of tan crystals, m.p. 203°-205°C
PAC 2-Methyl-6,7-dihydro-5H-pyrimido[5,4-d][1]benzazepineTo a solution of 0.308 g (3.26 mmol) of acetamidine hydrochloride in 15 ml of methanol under argon is added 0.176 g of (3.26 mmol) of sodium methoxide and the mixture stirred for 5 min. To the mixture is added 0.50 g (2.17 mmol) of 4-[(dimethylamino)methylene]-1,2,3,4-tetrahydro-5H-1-benzazepine-2,5-dione and the mixture stirred at room temperature overnight. The mixture (containing thick precipitate) is diluted with 3 ml of methanol, chilled and filtered. The filtrate is concentrated to dryness. The residue and original solid are combined and chloroform added. The mixture is washed with water, the organic layer is treated with activated carbon and then filtered through a thin pad of hydrous magnesium silicate. The filtrate is evaporated to give 0.41 g of crystals, m.p. 257°-258°C
The preceding compound is heated with 5 equivalents of lithium hydride in dioxane for 24 hours to give the product as a solid.
PAC 10,11-Dihydro-10-(4-nitrobenzoyl)dibenz[b,f][1,4]thiazepineTo a solution of 1.6 g of 10,11-dihydrodibenz[b,f][1,4]thiazepine in 30 ml of dichloromethane is added 0.1 g of 4-(N,N-dimethylamino)pyridine, 4 ml of triethylamine and 1.0 g of 4-nitrobenzoyl chloride and the mixture is stirred for 16 hours. The mixture is poured into ice-water and extracted with 3×150 ml of dichloromethane. The combined organic extract is washed with water, 2N HCl, 2N Na2 CO3 water and dried (MgSO4). The solvent is removed in vacuo and the product (2.6 g) purified by chromatography on silica gel with hexane-ethyl acetate (4:1) as eluent to give 2.2 g of crystals, m.p. 147°-149°C
PAC 10,11-Dihydro-10-(4-aminobenzoyl)dibenz[b,f][1,4]-thiazepineA mixture of 2.2 g of 10,11-dihydro-10-(4-nitrobenzoyl)dibenz[b,f][1,4]thiazepine in 150 ml of methanol is shaken in a Parr hydrogenator under 50 pounds per square inch of hydrogen for 72 hours. The mixture is filtered through diatomaceous earth and the filtrate evaporated in vacuo. The residue is re-crystallized from chloroform-hexane to give 1.8 g of crystals, m.p. 52°-55°C
PAC 4-[N-Methyl-N-(2-methylbenzoyl)amino]benzoic acidA sample of 1.51 g of sodium hydride (60% in oil) is washed with hexane under argon to remove the oil. To the washed sodium hydride is added 5 ml of N,N-dimethylformamide. To this mixture is added dropwise a solution of 8.69 g of ethyl 4-[(2-methylbenzoyl)amino]benzoate in 20 ml of N,N-dimethylformamide. The mixture is stirred at room temperature for 0.5 hour and then 5.23 g of methyl iodide is added. The mixture is stirred at room temperature for 16 hours. The mixture is diluted with water and extracted with dichloromethane. The extract is dried (Na2 SO4), concentrated to reduce the volume and the solution filtered through a thin pad of hydrous magnesium silicate. The filtrate is concentrated in vacuo to give 11 g of an oil (1:1 mixture of product and N,N-dimethylformamide). The preceding product, ethyl 4-[N-methyl-N-(2-methylbenzoyl)amino]benzoate, (11 g) is dissolved in 30 ml of methanol and 25 ml of 2N NaOH added. The mixture is refluxed for 2 hours and the solvent removed. The residue is extracted with ether (discard) and the remaining residue dissolved in 50 ml of water. The basic solution is acidified with 2N citric acid and the solid filtered off and washed with water. The product is air dried to give 6.72 g of crystals, m.p. 187°-190°C
PAC 4-[N-Methyl-N-(2-methylbenzoylamino]benzoyl chlorideA solution of 6.72 g of 4-[N-methyl-N-(2-methylbenzoyl)amino]benzoic acid in 20 ml of thionyl chloride is refluxed for one hour. The volatiles are removed in vacuo. Toluene is added to the residue and then the toluene removed in vacuo (repeated several times) to give the 7.3 g of product as a brown oil.
As described for Reference Example 60, but substituting the appropriate ethyl 4-[(N-aroyl)amino]benzoate, the following compounds are prepared.
PAC 4-[N-Methyl-N-(2-chlorobenzoyl)amino]benzoic acid PAC N-[N-Methyl-N-(2,5-dichlorobenzoyl)amino]benzoic acid PAC N-[N-Methyl-N-(2,4-dichlorobenzoyl)amino]benzoic acid PAC 4-[N-methyl-N-(2-chloro-4-methylbenzoyl amino]benzoic acid PAC 4-[N-methyl-N-(2-methyl-4-chlorobenzoyl)amino]-benzoic acid PAC 4-[N-Methyl-N-(2,4-dimethylbenzoyl)amino]benzoic acid PAC 4-[N-Methyl-N-(2,3-dimethylbenzoyl)amino]benzoic acid PAC 4-[N-Methyl-N-(2-methoxybenzoyl)amino]benzoic acid PAC 4-[N-Methyl-N-(2-trifluoromethoxybenzoyl)amino]benzoic acid PAC 4-[N-Methyl-N-(2,4-dimethoxybenzoyl)amino]benzoic acid PAC b 4-[N-Methyl-N-(2-methoxy-4-chlorobenzoyl)amino]benzoic acid PAC 4-[N-Methyl-N-(2-methylthiobenzoyl)amino]benzoic acid PAC 4-[N-Methyl-N-(2-methylthiophen-3-ylcarbonyl)amino]benzoic acid PAC 4-[N-Methyl-N-(3-methylthiophene-2-ylcarbonyl)amino]benzoic acid PAC 4-[N-Methyl-N-(2-methylfuran-3-ylcarbonyl)amino]benzoic acid PAC 4-[N-Methyl-N-(3-methylfuran-2-ylcarbonyl]amino]benzoic acid PAC 4-[N-Methyl-N-(phenylacetyl)amino]benzoic acid PAC 4-[N-Methyl-N-(2-chlorophenylacetyl)amino]benzoic acid PAC 4-[N-Methyl-N-(2-methoxyphenylacetyl)amino]benzoic acid PAC 4-[N-Methyl-N-(2-methylphenylacetyl)amino]benzoic acid PAC 4-[N-Methyl-N-(cyclohexylcarbonyl)amino]benzoic acid PAC 4-[N-Methyl-N-(3-cyclohexenecarbonyl)amino]benzoic acid PAC 4-[N-Methyl-N-(cyclohexylacetyl)amino]benzoic acid PAC 5,6-Dihydropyrido[2,3-b][1,4]benzothiazepineTo a suspension of 11.67 g of 2-thiobenzoic acid in a mixture of 32 ml of ethanol and 11 ml of water is added portionwise 12.72 g of solid sodium bicarbonate. After the complete addition, the mixture is stirred for 15 minutes and 10.0 g of 2-chloro-3-nitropyridine added portionwise. The mixture is refluxed for 2 hours, cooled and then concentrated in vacuo. The residual aqueous solution is diluted with 15 ml of water, acidified with 2N HCl and extracted twice with 250 ml of ethyl acetate. The extract is concentrated under vacuum to give a yellow solid residue. The residue is dissolved in a minimum of ethyl acetate by heating on a steam bath. The solution is cooled overnight and filtered. (2.5 g of starting material). The filtrate is concentrated, chilled and filtered to give 12.5 g of 2-(3-nitro-2-pyridinylthio)benzoic acid as a yellow solid. The preceding compound (5.0 g) and 0.75 g of Pd/C in 60 ml of ethanol is shaken in a Parr hydrogenator under 45 psi of hydrogen for 18 hours. The mixture is filtered through diatomaceous earth and the filter cake washed with 200 ml of dichloromethane. The combined filtrate is evaporated in vacuo to give a solid. The solid is triturated with ethanol and filtered to give 3.6 g of yellow solid. This solid (3.0 g) is again hydrogenated with Pd/c (0.50 g) in 50 ml of ethanol and 30 ml of acetic acid under 45 psi of hydrogen for 18 hours. The mixture is filtered through diatomaceous earth and the filter cake washed with methanol. The combined filtrate is concentrated in vacuo to give 1.6 g of solid. This solid in 25 ml of N,N-dimethylformamide is again reduced with 0.80 g of Pd/C under 45 psi of hydrogen to give 0.57 g of solid. Recrystallization from ethyl acetate gives 0.28 g of 2-(3-amino-2-pyridinylthio)benzoic acid. The preceding compound (0.20 g) is heated in 2-hydroxypyridine at 170°C to give 5,6-dihydropyrido[2,3-b][1,4]benzothiazepine as a yellow solid. The preceding compound is reacted with borane-dimethylsulfide as described for Reference Example 17 to give the product as a solid.
PAC 5,6-Dihydro-5-(4-aminobenzoyl)pyrido[2,3-b][1,4]benzothiazepineTo a mixture of 10 mmol of 5,6-dihydropyrido[2,3-b][1,4]benzothiazepine and 11 mmol of 4-nitrobenzoyl in 25 ml of dichloromethane chilled to 0° C. is added 15 mmol of triethylamine. The mixture is stirred at room temperature for 5 hours and then diluted with 75 ml of dichloromethane. The mixture is washed with H2 O, 2N citric acid, NaHCO3, brine and dried (Na2 SO4). The solvent is removed to give 5,6-dihydro-5-(4-nitrobenzoyl)pyrido[2,3-b][1,4]benzothiazepine as a solid. A mixture of the preceding compound (5 mmol), 0.3 g of Pd/C and 3 mmol of hydrazine in 25 ml of ethanol is refluxed for 3 hours. The mixture is filtered through diatomaceous earth and the filtrate evaporated in vacuo to give a solid. The solid is purified by chromatography on silica gel with ethyl acetate-hexane as solvent to give the product as a solid.
PAC 5,11-Dihydro-6-(4-aminobenzoyl)-6H-pyrido[2,3-e][1]benzazepineTo a mixture of 10 mmol of 5,11-dihydro-6H-pyrido[2,3-e][1]benzazepine, and 11 mmol of 4-nitrobenzoyl chloride in 25 ml of dichloromethane, chilled to 0°C, is added 15 mmol of triethylamine. The mixture is stirred at room temperature for 5 hours and diluted with 75 ml of dichloromethane. The mixture is washed with H2 O, 2N citric acid, NaHCO3, brine and dried (Na2 SO4). The solvent is removed in vacuo to give 5,11-dihydro-6-(4-nitrobenzoyl)-6H-pyrido[3,2-e][1]benzazepine as a solid. A mixture of the preceding compound (5 mmol) 0.3 g of Pd/C and 3 mmol of hydrazine in 25 ml of ethanol is refluxed 3 hours. The mixture is filtered through diatomaceous earth and the filtrate evaporated in vacuo to a solid. The solid is purified by chromatography on silica gel to give the product as a solid.
PAC 2-Nitro-2'-carboxy-diphenylamineA stirred solid mixture of 13.7 g of anthranilic acid, 20.2 g of o-bromonitrobenzene, 13.8 g of anhydrous potassium carbonate and 0.1 g of copper metal is heated 200°C oil bath. The reaction mixture is heated for 2 hours, cooled and the solid washed with ether (3×100 ml). The solid is dissolved in hot water and filtered. The filtrate is acidified with 40 ml of HCl and the resulting solid is collected and dried to give 20.5 g of the desired product as a solid, m.p. 262°-265°C
PAC 2-Amino-2'-carboxy-diphenylamineA solution of 7.3 g of 2-nitro-2'-carboxydiphenylamine in 50 ml of methanol containing 10% palladium-on-carbon is hydrogenated under 42 pounds of pressure for 24 hours. The reaction mixture is filtered through diatomaceous earth. The filtrate is evaporated to dryness in vacuo to give 6.6 g of the desired product as a solid, m.p. 72°-75°C
PAC 5,11-Dihydro-10H-dibenz[b,e][1,4]diazepine-11-oneA mixture of 6.6 g of 2-amino-2'-carboxydiphenylamine in 300 ml of xylene is heated at reflux for 20 hours. The xylene is evaporated in vacuo to a residue which is evaporated from 210 ml of toluene in vacuo to a residue which is evaporated from 50 ml of chloroform to give a residue. The residue is dissolved in 10 ml of tetrahydrofuran and added to 400 ml of ice-cold hexane. The resulting solid is collected, to give 4.3 g of the desired product as a solid, m.p. 121°-123°C
PAC 5,11-Dihydro-10H-dibenz[b,e][1,4]diazepineTo a stirred solution of 4.3 g of 5,11-dihydro-10H-dibenz[b,e][1,4]diazepin-11-one in 50 ml of tetrahydrofuran, under nitrogen and cooled to 0°C is added 4.0 ml of 10N methyl sulfide-borane complex. The ice bath is removed after 30 minutes and the reaction mixture stirred at room temperature for 18 hours. The reaction mixture is cooled in an ice bath and 30 ml of anhydrous methanol added dropwise and evaporated to dryness in vacuo. Another 30 ml of methanol is added and evaporated to a residue. The residue is quenched with 30 ml of 40% sodium hydroxide followed by heating at 110°C for 45 minutes and cooling to room temperature. The reaction mixture is diluted with 200 ml of water and extracted with methylene chloride (3×100 ml). The combined extracts are washed with 1N HCl, water and 0.5N NaOH. The organic layer is dried and evaporated in vacuo to give 3.2 g of the desired product, m.p. 114°-116°C
PAC 5H-Dibenz[b,e]azepine-6,11-dioneA mixture of 2.50 g of 2-aminobenzophenone2'-carboxylic acid in 50 ml of xylene is stirred at reflux for 23 hours. The mixture is filtered to give 1.82 g of the desired product as a solid.
PAC 2-Chloro-5H-dibenz[b,e]azepine-6,11-dioneA mixture of 1.0 g of 5H-dibenz[b,e]azepine-6,11-dione in 50 ml of acetic acid is stirred while chlorine is bubbled into the reaction mixture until saturated. The temperature increases to 38°C After standing, a precipitate forms and is filtered, washed with hexane and air dried to give 0.62 g of solid which is purified by chromatography to give the desired product as a solid, m.p. 289°-293°C
PAC 2-Chloro-6,11-Dihydro-5H-dibenz[b,e]azepineTo a mixture of 7.28 g of 2-chloro-5H-dibenz[b,e]azepine-6,11-dione in 25 ml of anhydrous tetrahydrofuran, under argon, is added dropwise 8.5 ml of (10M) boron-dimethyl sulfide. The reaction mixture is stirred at room temperature for 18 hours. The reaction mixture is heated at reflux for 3 hours and cooled to room temperature. While stirring, 25 ml of methyl alcohol is carefully added, followed by 100 ml of 2N NaOH. The reaction mixture is heated at reflux for 24 hours and the solid collected. The solid is dissolved in methylene chloride and washed with 2N citric acid, water and dried (Na2 SO4). The volatiles are evaporated in vacuo to give 4.16 g of a residue which is crystallized from ethyl acetate-hexane to give 2.05 g of the desired product as a crystalline solid, m.p. 137°-141°C
PAC 5,6-Dihydro-6-(4-nitrobenzoyl)-4H-isoxazolo[4,5-d][1]benzazepineA solution of 0.250 g of 1,2,3,4-tetrahydro-4-[(dimethylamino)methylene-1-(4-nitrobenzoyl)-5H-1-ben zazepin-5-one and 95.2 mg of hydroxylamine hydrochloride in 8 ml of methyl alcohol is heated at reflux under argon for 4 hours. The methanol is evaporated in vacuo to a residue which is dissolved in 10% ethyl acetate in methylene chloride and the solution passed through a pad of silica gel. The filtrate is evaporated in vacuo to give the desired product as a solid. CIMS:MH+ =336.
PAC 5,6-Dihydro-6-(4-aminobenzoyl)-4H-isoxazolo[4,5-d][1]benzazepineA mixture of 0.050 g of 5,6-dihydro-6-(4-nitrobenzoyl)-4H-isoxazolo[4,5-d][1]benzazepine and 0.169 g of SnCl2 2H2 O in 2 ml of ethyl alcohol is heated at reflux under argon for 1 hour. Water and 10% NaHCO3 is added until basic. The volatiles are evaporated in vacuo to a residue which is stirred with 1:1 chloroform-methanol and filtered. The filtrate is evaporated in vacuo to a residue which is dissolved in methyl alcohol, treated with activated carbon, filtered through diatomaceous earth and concentrated in vacuo to give 100 mg of the desired product as a white crystalline solid. CIMS(CH4):MH+ =306.
PAC 6,7-Dihydro-2-methyl-7-(4-nitrobenzoyl)-5H-pyrimido[5,4-d][1]benzazepineTo a stirred solution of 0.233 g of acetamidine hydrochloride in 36 ml of methyl alcohol under argon is added 0.133 g of NaOCH3. After 5 minutes, 0.600 g of 4-[(dimethylamino)methylene]-1,2,3,4-tetrahydro-1-(4-nitrobenzoyl)-5H-1-be nzazepin-5-one is added and stirring continued for 18 hours. The volatiles are evaporated to a residue which is dissolved in ethyl acetate and passed through a pad of silica gel. The filtrate is evaporated in vacuo to is give 590 mg of the desired product as tan crystals, m.p. 211°-212°C HR FABMS: Exact mass (M+H):361.1295.
PAC 6,7-Dihydro-2-methyl-7-(4-aminobenzoyl)-5H-pyrimido-[5,4-d][1]benzazepineA mixture of 400 mg of 6,7-dihydro-2-methyl-7-(4-nitrobenzoyl)-5H-pyrimido[5,4-d][l]benzazepine, 87 μl of anhydrous hydrazine and 40 mg of 10% Pd/C in 22 ml of ethyl alcohol is heated at reflux for 1.25 hours, filtered through diatomaceous earth and the pad washed well with methyl alcohol. The combined filtrates are evaporated in vacuo to a residue which is dissolved in ethyl acetate and filtered through a pad of hydrous magnesium silicate and the filtrate concentrated in vacuo to a residue which is dissolved in methyl alcohol and evaporated again to give 330 mg of the desired product as a yellow foam. HR FABMS: Exact mass (M+H): 331.1555.
PAC 4-[(Dimethylamino)methylene]-1,2,3,4-tetrahydro-1-(4-nitrobenzoyl)-5H-1-ben zazepin-5-oneA mixture of 1.35 g of 1,2,3,4-tetrahydro-1-(4-nitrobenzoyl)-5H-1-benzazepin-5-one and 15 ml of tert-butoxy-bis(dimethylamino)methane is heated for on a steam bath for 2 hours. The volatiles are evaporated in vacuo to a residue which is stirred with ether and filtered. The cake is washed with ether and the combined filtrates evaporated in vacuo to a residue which is dissolved in 30% ethyl acetate in methylene chloride and passed through a pad of hydrous magnesium silicate. The filtrate is evaporated in vacuo to a residue which is dissolved in 30% ethyl acetate in methylene chloride and passed through a pad of hydrous magnesium silicate. The filtrate is evaporated in vacuo to a residue to give 1.60 g of yellow crystalline product, m.p. 180°-183°C
PAC 2,4,5,6-Tetrahydro-2-methyl-6-(4-nitrobenzoyl)pyrazolo[4,3-d][1]benzazepineA solution of 0.150 g of 4-[(dimethylamino)methylene]-1,2,3,4-tetrahydro-1-(4-nitrobenzoyl)-5H-1-be nzazepin-5-one and 44 μl of methylhydrazine in 5 ml of methyl alcohol is heated at reflux for 18 hours. A precipitate forms on standing. The volatiles are evaporated to a residue which is purified by column chromatography on silica gel by elution with 5% ethyl acetate-methylene chloride. The product fractions are combined and the volatiles evaporated to a residue which is dissolved in chloroform-methanol, filtered through glass wool and the filtrate evaporated in vacuo to give 0.110 g of the desired product as pale yellow crystals.
PAC 2,4,5,6-Tetrahydro-2-methyl-6-(4-nitrobenzoyl)pyrazolo[4,3-d][1]benzazepineA mixture of 0.520 g of 2,4,5,6-tetrahydro-2-methyl-6-(4-nitrobenzoyl)pyrazolo[4,3-d][1]benzazepin e, 118 μl of anhydrous hydrazine and 52 mg of 10% palladium-on-carbon in 30 ml of absolute ethyl alcohol is heated at reflux for 1 hour. The reaction mixture is filtered through diatomaceous earth and the cake washed with 100 ml of methyl alcohol and 1:1 chloroform-methyl alcohol to give 430 mg of the desired product as off-white crystals. CIMS(CH4)MH+ =319.
PAC 5-(2-Chloro-4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepineA mixture of 1.40 g of 5-(2-chloro-4-nitrobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.20 g of 10% palladium-on-carbon, 0.25 g of anhydrous hydrazine in 25 ml of absolute ethanol is heated at reflux for 1 hour. The mixture is filtered through diatomaceous earth and the filtrate evaporated in vacuo to a residue which is dissolved in methylene chloride and hexane added at the boil to give 0.60 g of the desired product as a crystalline solid, m.p. 158°-161°C
PAC 2-[2-(Tributylstannyl)-3-thienyl]-1,3-dioxolaneTo a stirred solution of 15.6 g (0.10 mol) of 2-(3-thienyl)-1,3-dioxolane in 100 ml of anhydrous ether, n-butyl-lithium (1.48N, in hexane, 74.3 ml) is added dropwise under nitrogen at room temperature. After being refluxed for 15 minutes, the reaction mixture is cooled to -78°C and tri-n-butyltin chloride (34.18 g, 0.105 mol) in 100 ml of dry tetrahydrofuran is added dropwise. After the addition is complete, the mixture is warmed to room temperature and the solvent evaporated. To the oily residue 100 ml of hexane is added, and the resulting precipitate (LiCl) is filtered off. The filtrate is evaporated and the residue distilled at reduced pressure, giving 34.16 g (77%) of the desired product.
PAC 2-[2-[(2-Nitrophenyl)methyl]-3-thienyl1-1,3-dioxolaneA mixture of 2-[2-(tributylstannyl)-3-thienyl]-1,3-dioxolane (8.8 gms, 20 mmols), 2-nitrobenzyl bromide (4.5 gms, 22 mmol) and tetrakis (triphenylphosphine)-palladium (0) (200 mg) is refluxed in degassed toluene for 16 hours under nitrogen atmosphere. At the end, the reaction mixture is cooled to room temperature and filtered through diatomaceous earth. The toluene is removed by concentrating at reduced pressure and the product isolated by silica gel column chromatography by elution with 30% ethyl acetate:hexane to give 4.5 gms. of the desired product as a viscous liquid. Mass Spectrum; M+ 292.
PAC 4,10-Dihydro-5H-thieno[3,2-c][1]benzazepineA stirred solution of 4 gms of 2-[2-[(2-nitrophenyl)methyl]-3-thienyl]-1,3-dioxolane in acetone (50 ml) and acetic acid (90% 50 ml) is heated to 60°C Zinc dust (10 gms) is slowly added and after the addition, reaction mixture is stirred for 6 hours. At the end, reaction mixture is filtered and the residue washed with acetone and concentrated. The brown residue is extracted with chloroform and washed well with water. The organic layer is dried (Na2 SO4) and filtered and concentrated. The product is isolated by silica gel column chromatography by eluting with 20% ethyl acetate:hexane to give 2.0 g of the desired product as a pale yellow crystalline solid, m.p. 86°C Mass Spectrum; M+ 202.
PAC 4,5-Dihydro-4,4-dimethyl-2-[3-[(2-nitrophenyl)methyl]-2-thienyl]oxazoleTo a solution of 4,5-dihydro-4,4-dimethyl-2-(2-thienyl)-oxazole (4.5 gms 25 mmol) in anhydrous ether at -70°C, n-butyl-lithium (2.5 molar solution in hexane, 11 ml) is added drop by drop under N2 atmosphere. The reaction mixture is stirred at --78°C for 45 minutes and tri-n-butyltin chloride (8.3 gms 25 mmol) in dry ether is added drop by drop. The reaction mixture is stirred at room temperature for 1 hour and quenched with water. The reaction mixture is extracted with ether, washed well with water, dried and concentrated. The product obtain is pure enough for further transformation. The oil product, 4,5-dihydro-4,4-dimethyl-2-[3-(tributylstannyl)-2-thienyl]-oxazole is mixed with 2-nitrobenzyl bromide (5.5 g 25 mmol) in toluene and refluxed in the presence of tetrakis (triphenylphosphine)-palladium (0) (200 mg) for 16 hours. At the end, reaction mixture is cooled to room temperature and filtered. Toluene is removed under reduced pressure and the product is isolated as brown oil by silica gel column chromatography by eluting it with 30% ethyl acetate:hexane to give 5.7 g of the desired product. Mass Spectrum; M+ 316.
PAC 9,10-Dihydro-4H-thieno[3,2-c][1]benzazepin-10-oneA solution of 4,5-dihydro-4,4-dimethyl-2-[3-[(2-nitrophenyl)methyl]-2-thienyl]oxazole 5 gms is refluxed in acetone/water (3:1 100 ml) containing 1N HCl (30 ml) for 24 hours. The reaction mixture is concentrated and the residue is dissolved in glacial acetic acid (100 ml). The acetic acid is stirred at 70°C and zinc dust (10 gm) is slowly added. Stirring is continued at 70°C for 6 hours. At the end, the reaction mixture is cooled to room temperature and filtered. Acetic acid is removed under reduced pressure and the residue is extracted with chloroform. The chloroform layer is dried and concentrated to give 2.9 gms of the desired product as a brown solid. Mass Spectrum; M+ 215.
PAC 9,10-Dihydro-4H-thieno[3,2-c][1]benzazepineA stirred solution of 2.0 g of 9,10-dihydro-4H-thieno[2,3-c][1]benzazepin-10-one and lithium aluminum hydride (500 mg) in tetrahydrofuran is refluxed for 4 hours. At the end, reaction mixture is carefully quenched with ice cold water and extracted with chloroform. The organic layer is washed well with water and dried over anhydrous Na2 SO4, filtered and concentrated. The product is purified by silica gel column chromatography by eluting it with 30% ethyl acetate:hexane to give 1.2 g of the desired product as a bright yellow solid. Mass Spectrum; M+ 202.
PAC 4-Bromo-1,2,3,4-tetrahydro-1-(4-nitrobenzoyl)-5H-1-benzazepin-5-oneA mixture of 0.200 g of 1,2,3,4-tetrahydro-1-(4-nitrobenzoyl)-5H-1-benzazepin-5-one in 2.5 ml of acetic acid is warmed until solution then allowed to cool to room temperature. While stirring, a solution of 0.103 g of bromine in 0.5 ml of acetic acid is added dropwise. After rapid decolorization, the reaction mixture is stirred for 1.5 hours and poured into water. The solid is collected, washed with water and air dried to give 220 mg of the desired product as a crystalline solid, Mass Spectrum; MH+ =389,391.
PAC 5,6-Dihydro-2-methyl-6-(4-nitrobenzoyl)-4H-thiazolo[5,4-d][1]benzazepineA mixture of 1.19 g of 4-bromo-1,2,3,4-tetrahydro-1-(4-nitrobenzoyl)-5H-1-benzazepin-5-one and 0.230 g of thioacetamide in 4 ml of ethyl alcohol is refluxed under argon for 18 hours. The volatiles are evaporated in vacuo to a residue which is partitioned between CHCl3 and 10% NaHCO3. The organic layer is separated and washed twice with water. The organic layer is separated, dried (MgSO4) and evaporated in vacuo to give 1.08 g of yellow foam which is purified by flash chromatography on silica by elution with 4% ethyl acetate in methylene chloride to give 560 mg of the desired product as pale yellow foam.
PAC 5,6-Dihydro-2-methyl-6-(4-aminobenzoyl)-4H-thiazolo[5,4-d][1]benzazepineA mixture of 0.080 g of 5,6-dihydro-2-methyl-6-(4-aminobenzoyl)-4H-thiazolo[5,4-d][1]benzazepine and 0.248 g of SnCl2 dihydrate in 3.5 ml of ethyl alcohol is refluxed under argon for 1 hour. The reaction mixture is diluted with ice water and the pH adjusted to 8 with 10% NaHCO After stirring for 3 hours, the mixture is extracted with CHCl3 (3×). The combined organic layers are treated with activated carbon, filtered through a pad of MgSO4 and the filtrate evaporated in vacuo to a residue. The residue is chromatographed on silica gel by elution with 30% ethyl acetate in methylene chloride to give 60 mg of the desired product as a tan solid, CIMS(CH4):MH+ =336.
PAC Methyl 4-[2-(2-chlorophenyl)-2-cyano-2-(4-morpholinyl)ethyl]benzoateA 0.876 g sample of 60% sodium hydride in oil is washed with hexane followed by the addition of 60 ml of dry N,N-dimethylformamide. The reaction mixture is stirred for 1 hour under argon at room temperature after the addition of 4.73 g of α-(2-chlorophenyl)-4-morpholineacetonitrile. To the reaction mixture is added 4.58 g of methyl 4-(bromomethyl)benzoate and stirring continued for 3 hours. Several drops of acetic acid is added to ice water and the reaction quenched. The pH is 3-4 and saturated NaHCO added to adjust the pH to 6-7. Upon cooling a solid forms which is filtered, washed with water and dried to give 5.92 g of yellow solid. Crystallization from methylene chloride-hexane gives 2.10 g of the desired product as a crystalline solid, m.p. 116°-118°C
PAC Methyl 4-[2-(2-chlorophenyl)-2-oxoethyl]benzoateA mixture of 1.0 g of methyl [4-(2-chlorophenyl)-2-cyano-2-(4-morpholinyl)ethyl]benzoate and 14 ml of acetic acid and 6 ml of water is heated at reflux for 20 minutes then poured over crushed ice. After stirring for 15 minutes the resulting solid is collected, washed with water and air dried to give 0.63 g of tan solid, m.p. 40°-42°C
PAC 4-[2-(2-chlorophenyl)-2-oxoethyl]benzoic acidA mixture of 18.78 g of methyl 4-[2-(2-chlorophenyl)-2-oxoethyl]benzoate in 288.8 ml of CH3 OH, 72.2 ml of water and 5.2 g of NaOH is refluxed for 3 hours then acidified with 2N citric acid. The reaction mixture is evaporated in vacuo to remove the CH3 OH. The aqueous phase is extracted with CH2 Cl2 and acidified with 1N HCl. The resulting solid is collected and dried under vacuum to give 17.27 g of the desired product, m.p. 168°-172°C
PAC N-[4-[(10,11-dihydro-5H-dibenz[b,f]azepin-5-yl)carbonyl]phenyl]-2-methylben zamideTo a mixture of 1.37 g (5 mmol) of 4-[(2-methylbenzoyl)amino]benzoyl chloride and 0.061 g of 4-(dimethylamino)pyridine in 4 ml of pyridine is added 0.975 g (5 mmol) of 10,11-dihydro-5H-dibenz[b,f]azepine. The mixture is heated at 80°C for 18 hours and then 0.2 g of sodium hydride (60% in oil) (5 mmol) is added. The mixture is refluxed for 2 hours, diluted with dichloromethane and water and then filtered. To the filtrate is added 1N HCl and the mixture filtered. The filtrate is dried (Na2 SO4) and the solvent removed to give a solid. The solid (1.1 g) is chromatographed on thick layer silica gel plates to give 70 mg of yellow solid, m.p. 112°-118°C
Anal. Calc'd for C29 H24 N2 O: C,80.5; H,5.6; N,6.5. Found: C,78.7; H,5.8; N,6.7.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-2-methylbenz amideTo a solution of 0.27 g (1 mmol) of 4-[(2-methylbenzoyl)amino]benzoyl chloride in 2 ml of tetrahydrofuran is added 0.20 g (1 mmol) of 6,11-dihydro-5H-dibenz[b,e]azepine and 0.20 g of triethylamine. The mixture is stirred at room temperature for 3 hours and the solvent removed under vacuum. To the residue is added 1N HCl and the mixture extracted with ethyl acetate (20 ml) and the extract washed with saturated NaHCO3, brine and dried (Na2 SO4). The solution is filtered through a thin pad of hydrous magnesium silicate and the filtrate evaporated. The residue is triturated with ether-hexane and filtered to give 0.47 g of a white solid: Mass Spectrum; EI 433 (M+1); EI-high resolution 432.1842.
Anal. Calc'd for C29 H24 N2 O2 : C,80.5; H,5.6; N,6.5. Found: C,79.0; H,6.0; N,6.1. A sample crystallized from ethyl acetate-hexane gives crystals, m.p. 198°-203 °C
PAC 3-Methyl-N-[4-[(5(6H)-phenanthridinyl)carbonyl]-phenyl]-2-thiophenecarboxam ideTo 0.193 g (1.2 mmol) of 3-methylthiophene-2-carbonyl chloride in 3 ml of dichloromethane, cooled to 0°C, is added 209 μl of triethylamine. The mixture is stirred and 0.30 g (1 mmol) of 5-(4-aminobenzoyl)-5,6-dihydrophenanthridine is added. The mixture is stirred at room temperature overnight and then concentrated under vacuum. To the residue is added 30 ml of ethyl acetate and the mixture washed with 2 ml each of water, 2N citric acid, IM sodium bicarbonate and brine. The organic layer is dried (Na2 SO4) and the solvent removed to give 0.30 g of solid. The solid is chromatographed on thick layer silica gel plates with ethyl acetate-hexane (1:1) as solvent to give 150 mg of product as a yellow foam.
Anal. Calc'd for C26 H20 N2 O2 S: C,71.3; H,6.0; N,6.9; S,7.9. Found: C,71.0; H,5.8; N,6.8; S,7.8.
PAC N-[4-[(2-Chlorodibenz[b,f][1,4oxazepin-10(11H)-yl)carbonyl1phenyl]-2-methyl benzamideTo a mixture of 0.229 g (1.0 mmol) of 2-chloro-10,11-dihydrodibenz[b,f][1,4]oxazepine in 1.0 ml of pyridine under nitrogen is added 0.30 g (1.1 mmol) of 4-[(2-methylbenzoyl)amino]benzoyl chloride. The mixture is stirred at room temperature for 1 hour, heated on a steam bath for 5 minutes and 8 ml of 2N HCl added. The mixture is extracted with ethyl acetate and the extract washed three times with 1 ml of 1N sodium bicarbonate. The organic layer is dried (MgSO4) and the solvent removed. The residue is crystallized from ethyl acetate-hexane to give 0.24 g of crystals, m.p. 207°-208°C Anal. Calc'd for C28 H2 N2 O3 Cl: C,71.9; H,4.5; N,6.0; Cl,7.6. Found: C,71.6; H,4.6; N,5.9; Cl,7.4.
PAC 2-Methyl-N-[4-[(5(6H)-phenanthridinyl)carbonyl]phenyl]benzamideTo a solution of 0.181 g (1.0 mmol) of 5,6-dihydrophenanthridine in 2 ml of warm pyridine is added 0.273 g (1.0 mmol) of 4-[(2-methylbenzoyl)amino]benzoyl chloride. The mixture is stirred overnight at room temperature, 1.2 ml of 2N HCl added. The solid which separates is filtered and washed with water. The solid is dissolved in dichloromethane and the solution washed with 2M sodium carbonate. The organic layer is concentrated and the residue chromatographed twice on silica gel with ethyl acetate-hexane as solvent to give 79 mg of crystals, m.p. 190°-194°C Mass Spec-FAB 419 (M+H).
Anal. Calcd for C28 H20 N2 O2 H2 O: C,77.4; H,5.l; N,6.4. Found: C,77.5; H,5.1; N,6.4.
PAC N-[4-[(11,12-Dihydrodibenz[b,f]azocin-5-(6H)-yl)-carbonyl]phenyl]-2-methylb enzamideTo a mixture of 0.245 g (1 mmol) of 5,6,11,12-tetrahydrodibenz[b,f]azocine hydrochloride and 30 μl (2.2 mmol) of triethylamine in 2 ml of dichloromethane is added a solution of 0.281 g (1.1 mmol) of 4-[(2-methylbenzoyl)amino]benzoyl chloride in 4 ml of dichloromethane. The mixture is stirred overnight at room temperature, washed with water 2N HCl (3×2 ml) and 1N sodium bicarbonate (3×2 ml). The organic layer is dried (MgSO4) and filtered through a thin pad of hydrous magnesium silicate (pad washed with 3 volumes of CH2 Cl2). The filtrate is concentrated to give 200 mg of a foam; Mass Spec.-FAB 447 (M+H).
Anal. Calc'd for C30 H26 N2 O2 : C,80.7; H,5.9; N,6.3. Found: C,79.1; H,5.7; N,6.1.
PAC 2,6-Dichloro-N-[4-[(5(6H)-phenanthridinylcarbonyl]phenyl1benzamideA mixture of 300 mg (0.5 mmol) of 5-(4-aminobenzoyl)-5,6-dihydrophenanthridine and 230 mg of (0.55 mmol) of 2,6-dichlorobenzoyl chloride in 1.2 ml of pyridine is heated (100° C.) for 3 hr and then stirred at room temperature for 6 days. To the mixture is added 10 mg of 4-(dimethylamino)pyridine and the mixture stirred for 22 days. To the mixture is added 6 ml of 2N HCl and the solid filtered and washed with 2N HCl, 1N NaOH, H2 O to give 0.57 g of solid. The solid is chromatographed on thick layer silica gel plates with hexane ethyl-acetate (1:1) to give 110 mg of solid. Recrystallization from CH2 Cl2 -diisopropyl ether to give 73 mg of white crystals, m.p. 230°-235°C
Anal. Calc'd for C27 H18 Cl2 N2 O2 H2 O: C,66.0; H,4.1; N,5.7; Cl,14.4. Found: C,65.5; H,4.1; N,5.6; Cl,14.6.
PAC 3.4-Dichloro-N-[4-[(5(6H)-phenanthridinyl)carbonyl]phenyl]benzamideA mixture of 150 mg (0.5 mmol) of 5-(4-aminobenzoyl)-5,6-dihydrophenanthridine and 115 mg (0.55 mmol) of 3,4-dichlorobenzoyl chloride in 1 ml of pyridine is stirred at room temperature for 6 hours. To the mixture is added 6 ml of 2N HCl and the mixture stirred and filtered to give a solid. The solid is washed with water, 2N sodium carbonate and water to give 254 mg of crystals, m.p. 94°-95°C The solid is chromatographed on thick layer silica gel plates with hexane ethyl-acetate (1:1) as solvent to give 107 mg of solid. Mass Spec (FAB) 473 (M+H).
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-2,5-dichloro benzamideA mixture of 0.625 g (2 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.629 g (3 mmol) of 2,5-dichlorobenzoyl chloride, 0.303 g (3 mmol) of triethylamine and 15 mg of 4-(dimethylamino)pyridine in 10 ml of dichloromethane is stirred at room temperature for 3 hours. The mixture is washed with water, 1N HCl, H2 O, 1M NaHCO3, brine and dried (Na2 SO4). The solvent is removed to give crystals. Recrystallization from hexane-CH2 Cl2 gives 0.16 g of white crystals, m.p. 203°-231°C
Anal. Calc'd for C28 H20 Cl2 N2 O2 : C,69.0; H,4.1; N,5.8; Cl,14.6. Found: C,69.0; H,3.8; N,5.6; Cl,14.8.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]2,4-dichlorob enzamideAs described for Example 9, 0.111 g (1.1 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine in 8 ml of dichloromethane is reacted with 0.230 g of (1.1 mmol) of 2,4-dichlorobenzoyl chloride. The product is recrystallized from hexane-dichloromethane to give 0.24 g of crystals, m.p. 212°-215°C
Anal. Calc'd for C18 H20 Cl2 N2 O2 H2 O: C,66.5; H,4.4; N,5.5. Found: C,66.8; H,4.0; N,5.5.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-2,3-dimethyl benzamideAs described for Example 9, 0.628 g (2 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine is reacted with 0.506 g (3.0 mmol) of 2,3-dimethylbenzoyl chloride in dichloromethane. The product is recrystallized from hexane-dichloromethane to give 0.12 g of crystals, m.p. 138°-142°C
Anal. Calc'd for C30 H26 N2 O2 : C,80.7; H,5.9; N,6.3. Found: C,80.0; H,5.9; N,6.1.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-2,5-dimethyl benzamideAs described for Example 9, 0.471 g (1.5 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine is reacted with 0.303 g (1.8 mmol) of 2,5-dimethylbenzoyl chloride in 10 ml of dichloromethane. The product is recrystallized from dichloromethane-hexane to give 0.43 g of crystals, m.p. 213°-216°C Anal. Calc'd for C30 H26 N2 O2 : C,80.7; H,5.9; N,6.3. Found: C,80.0; H,5.9; N,6.1.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]2,4-dimethylb enzamideAs described in Example 9, 0.471 g (1.5 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine is reacted with 0.303 g (1.8 mmol) of 2,4-dimethylbenzoyl chloride in 10 ml of dichloromethane. The product is recrystallized from hexane-dichloromethane to give 0.38 g of crystals, m.p. 197°-199°C
Anal. Calc'd for C30 H26 N2 O2 1/2 H2 O: C,79.1; H,6.0; N,6.2. Found: C,79.0; H,5.8; N,6.2.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-2-chloroben zamideAs described in Example 9, 0.471 g (1.5 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz b,e]azepine is reacted with 0.315 g (1.8 mmol) of 2-chlorobenzoyl chloride in dichloromethane. The product is chromatographed on thick layer silica gel plates with hexane-ethyl acetate (1:1) as solvent to give a solid. Recrystallization from hexane-dichloromethane gives 100 mg of crystals, m.p. 110°-115°C Anal. Calc'd for C28 H21 ClN2 O2 1/2 H2 O: C,72.8; H,4.8; N,6.1; Cl,7.7. Found: C,72.6; H,4.5; N,5.8; Cl,8.7.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-2-methylbenz amideAs described for Example 9, 0.942 g (3 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz b,e]azepine is reacted with 0.52 g (3.3 mmol) of 2-methylbenzoyl chloride in 20 ml of dichloromethane. The product is triturated with hexane-ethyl acetate to give 1.0 g of yellow crystals, m.p. 198°-203°C
PAC 2-Chloro-N-[4-[(6,11-dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]benz eneacetamideA mixture of 0.471 g (1.5 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.340 g (1.8 mmol) of 2-chlorophenylacetyl chloride, 0.20 g of triethylamine and 9 mg of 4-(dimethylamino)pyridine in 10 ml of dichloromethane is stirred at room temperature for 48 hours. An additional 0.27 g of 2-chlorophenylacetyl chloride is added and the mixture stirred at room temperature for 2.5 hr. The mixture is washed with 1N HCl, H2 O, 1M NaHCO3, brine and dried (Na2 SO4). The solvent is removed and the solid recrystallized from dichloromethane to give 0.27 g of crystals, m.p. 191°-194°C Anal. Calc'd for C29 H23 ClN2 O2 : C,74.6; H,5.0; N,6.0; Cl,7.6. Found: C,74.4; H,4.9; N,5.9; Cl,7.8.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-3-pyridinec arboxamideA mixture of 0.628 g (2 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.551 g (3 mmol) of nicotinoyl chloride, hydrochloride, 0.606 g (6 mmol) of triethylamine and 15 mg of 4-(dimethylamino)pyridine in 17 ml of dichloromethane is refluxed for 7 hours. The mixture is washed with H2 O, 2N citric acid, H2 O, 1N NaHCO3, brine and dried (Na2 SO4). The solvent is removed and the solid recrystallized from hexane-dichloromethane to give 0.12 g of crystals,. m.p. 217°-220°C Anal Calc'd for C27 H21 N3 O2 H2 O: C,74.1; H,5.3; N,9.6. Found: 73.6; H,4.7; N,9.8.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-3-methyl-2-t hiophenecarboxamideAs described for Example 9, 0.314 g (1 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine is reacted with 0.177 g (1.1 mmol) of 3-methyl-2-thiophenecarbonyl chloride in 5 ml of dichloromethane and 0.111 g of triethylamine for 2 hours at room temperature to give crystals. Recrystallization from dichloromethane-hexane gives 0.235 g of crystals, m.p. 201°-204°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepine-5-yl)carbonyl]phenyl]-3-(trifluor omethyl)benzamideAs described for Example 9, 0.314 g (1 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz b,e]azepine is reacted with 0.302 g (1.4 mmol) of 3-(trifluoromethyl)benzoyl chloride in 9 ml and 0.145 g (1.4 mmol) of triethylamine for 1.5 hour at room temperature. The product is recrystallized from ethyl acetate-hexane to give 0.14 g of crystals, m.p. 190°-191°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-4-(trifluoro methyl)benzamideAs described for Example 9, 0.314 g of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine is reacted with 0.269 g (1.29 mmol) of 4-trifluoromethylbenzoyl chloride and 0.130 g (1.29 mmol) of triethylamine in 9 ml of dichloromethane for 1.5 hours at room temperature. The product is triturated with ethyl acetate-hexane to give 0.43 g of crystals, m.p. 205°-207°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-2,4-difluoro benzamideAs described for Example 9, 0.314 g (1.0 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine is reacted with 0.194 g (1.1 mmol) of 2,4-difluorobenzoyl chloride and 0.111 g (1.1 mmol) of triethylamine in 10 ml of dichloromethane for 1.5 hours at room temperature. The product is recrystallized from ethyl acetate-hexane to give 0.37 g of crystals, m.p. 215°-217°C
PAC N-[4-[6,11-Dihydro-11-methyl-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-2-m ethylbenzamideA sample of 6,11-dihydro-11-methyl-5H-dibenz[b,e]azepine is synthesized as described in J. Chem. Soc. Perkin I, 1279 (1976). A 0.210 g (1 mmol) sample is added to a stirred and cooled mixture of 0.328 g (1.2 mmol) of 4-[(2-methylbenzoyl)amino]benzoyl chloride, 279 mL (2.0 mmol) of triethylamine and 26 mg of 4-(dimethylamino)pyridine in 4 ml of dichloromethane. The solution is stirred at room temperature overnight. An additional 0.328 g of 4-[(2-methylbenzoyl)amino]benzoyl chloride and 150 μl of triethylamine is added and the mixture stirred at room temperature for 6 hours. The volatiles are removed and 30 ml of ethyl acetate is added. The mixture is washed with 12 ml each of 2N citric acid, H2 O, 1M NaHCO3, brine and dried (Na2 SO4). The solvent is removed and the residue chromatographed on thick layer silica gel plates with hexane-ethyl acetate (2:1) as solvent to give 0.13 g of product as a white solid. Anal. Calc'd for C30 H26 N2 O2 1/4 H2 O: C,79.9; H,5.9; N,6.2. Found: C,79.4; H,5.5; N,5.9.
PAC N-[4-[[2-[(Dimethylamino)sulfonyl]dibenz[b,f][1,4]oxazepin-10(11H)-yl]carbo nyl]phenyl]-2-methylbenzamideA solution of 0.22 g of 10,11-dihydro-N,N-dimethyl-10-(4-nitrobenzoyl)dibenz[b,f]1,4]oxazepine-2-s ulfonamide, 50 mg of 10% Pd/C under an atmosphere of H2 is shaken in a Parr hydrogenator for 5 hours. The mixture is filtered through diatomaceous earth and the filter cake washed with ethyl acetate. The filtrate is concentrated to 5a, 6 ml and 0.83 μl of triethylamine added followed by the addition of 0.773 g of o-tolyl chloride. The mixture is stirred overnight and then washed with H2 O, 2N HCl, 1M Na2 CO3 and brine. The filtrate is filtered through a thin pad of hydrous magnesium silicate and the pad washed with three volumes of ethyl acetate. The filtrate is concentrated under vacuum and the residual oil chromatographed on thick layer silica gel plates with hexane-ethyl acetate (1:1) to give 83 mg of a foam. Mass Spectrum (FAB) 540(M+H).
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]benzamideAs described for Example 9, 0.314 g (1.0 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine is reacted with 0.155 g (1.1 mmol) of benzoyl chloride and 0.111 g (1.1 mmol) of triethylamine in 10 ml of dichloromethane for 1.5 hours at room temperature. The product is recrystallized from dichloromethane-hexane to give 0.19 g of crystals, m.p. 219°-221°C Anal. Calc'd for C28 H22 N2 O2 : C,80.4; H,5.3; N,6.7. Found: C,79.6; H,5.5; N,6.7.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-2-(trifluoro methoxy)benzamideAs described for Example 9, 0.314 g (1.0 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine is reacted with 0.247 g (1.1 mmol) of 2-(trifluoromethoxy)benzoyl chloride and 0.111 g (1.1 mmol) of triethylamine in 10 ml of dichloromethane at room temperature for 1.5 hours. The product is triturated with dichloromethane-hexane to give 0.35 g of crystals, m.p. 232°-235°C
The following compounds are prepared as described for in Example 6.
PAC N-[4-[(11,12-Dihydrodibenz[b,f]azocin-5(6H)-yl)carbonyl]phenyl]-2-chloroben zamide PAC N-[4-[(11,12-Dihydrodibenz[b,f]azocin-5(6H)-yl)carbonyl]phenyl]-2-fluoroben zamide PAC N-[4-[(11,12-Dihydrodibenz[b,flazocin-5(6H)-yl)-carbonyl]phenyl]-2-methoxyb enzamide PAC N-[4-[(11,12-Dihydrodibenz[b,flazocin-5(6H)-yl)-carbonyl]phenyl1-2,3-dimeth ylbenzamide PAC N-[4-[(11,12-Dihydrodibenz[b,flazocin-5(6H)-yl)-carbonyl]phenyl]-1-2,5-dime thylbenzamide PAC N-[4-[(11,12-Dihydrodibenz[b,f]azocin-5(6H)-yl)-carbonyl]phenyl]-2,4-dichlo robenzamide PAC N-[4-[(11,12-Dihydrodibenz[b,f]azocin-5(6H)-yl)-carbonyl]phenyl]-2,3-dichlo robenzamide PAC N-[4-[(11,12-Dihydrodibenz[b,f]azocin-5(6H)-yl)-carbonyl]phenyl]-2-methyl-5 -fluorobenzamide PAC N-[4-[(11,12-Dihydrodibenz[b,f]azocin-5(6H)-yl)-carbonyl]phenyl]-2-chloroph enylacetamideThe following compounds are prepared as described in
PAC EXAMPLE 35 PAC EXAMPLE 36 PAC EXAMPLE 37 PAC EXAMPLE 38 PAC EXAMPLE 39 PAC EXAMPLE 40 PAC EXAMPLE 41 PAC EXAMPLE 42 PAC EXAMPLE 43 PAC EXAMPLE 44 PAC EXAMPLE 45 PAC EXAMPLE 46 PAC EXAMPLE 47 PAC EXAMPLE 48 PAC EXAMPLE 49The following compounds are prepared as described in Example 4.
PAC N-[4-[(2-Chlorodibenz[b,f][1,4]oxazepin-10(11H)-yl)-carbonyl]phenl]-2-chlor obenzamide PAC N-[4-[(2-Chlorodibenz[b,f][1,4]oxazepin-10(11H)-yl)-carbonyl]phenyl]-2-fluo robenzamide PAC N-[4-[(2-Chlorodibenz[b,f][1,4]oxazepin-10(11H)-yl)-carbonyl]phenyl]-2-meth oxybenzamide PAC N-[4-[(2-chlorodibenz[b,f][1,4]oxazepin-10(11H)-yl)-carbonyl]phenyl1-2,3-di methylbenzamide PAC N-[4-[(2-Chlorodibenz[b,f][1,4]oxazepin-10(11H)-yl)-carbonyl]phenyl]-2,5-di methylbenzamide PAC N-[4-[(2-Chlorodibenz[b,f][1,4]oxazenin-10(11H)-yl)-carbonyl]phenyl]-2,4-di chlorobenzamide PAC N-[4-[(Dibenz[b,f][1,4]oxazepin-10(11H)-yl)-carbonyl]phenyl]-2,3-dichlorobe nzamide PAC N-[4-[(Dibenz[b,f][1,4]oxazeyin-10(11H)-yl)-carbonyl]phenyl]-2-chlorophenyl acetamide PAC N-[4-[(Dibenz[b,f][1,4]oxazeyin-10(11H)-yl)-carbonyl]phenyl1-2-methyl-3-thi ophenecarboxamide PAC N-[4-[(Dibenz[b,f][1,4]oxazepin-10(11H)-yl)-carbonyl]phenyl]-2-methylphenyl acetamide PAC N-[4-[(Dibenz[b,f][1,4]oxazepin-10(11H)-yl)-carbonyl]phenyl]-2-methyl-4-chl orobenzamide PAC N-[4-[(6.11-Dihydro-5H-dibenz[b.e]azepin-5-yl)-carbonyl]phenyl]-2-(methylth io)benzamideAs described for Example 9, a mixture of 0.242 g of 2-(methylthio)benzoyl chloride (m.p. 61°-640°C), 0.134 g of triethylamine and 0.314 g of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine in 10 ml of dichloromethane is stirred for 2.5 hours and worked up to give a solid. Trituration with ethyl acetate-hexane gives 0.150 g of crystals, m.p. 222°-225°C
PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl) carbonyl]-phenyl]-2-methylbenzamideAs described for in Reference Example 12, a mixture of 3.3 g of 10,11-dihydro-11-oxodibenz[b,f] [1,4]thiazepine, 25 ml of tetrahydrofuran, 4.0 ml of 10 molar borane-dimethylsulfide (2.67 equiyalents) in tetrahydrofuran is stirred at room temperature 18 hours to give, after work-up, 10,11-dihydrodibenz[b,f][1,4] thiazepine as white crystals, m.p. 145°-148°C The preceding compound (3.5 g) is suspended in 25 ml of dichloromethane and a solution of 1.8 g of 4-[(2-methylbenzoyl) amino]benzoyl chloride in 50 ml of di-chloromethane added. To the stirred mixture is added 4 ml of triethylamine and 0.2 g of 4-(dimethylamino) pyridine. The mixture is stirred at room temperature for 20 hours. The mixture is filtered and the filtrate concentrated. The residue is purified by chromatography on silica gel with hexane-chloroform-ethyl acetate (2:1:1) as solyent to give 2.2 g of yel]ow crystals. A sample (0.80 g) is further purified by thick layer chromatography on silica gel with hexane-chloroform-ethyl acetate (2:1:1) as solvent to give 0.50 g of crystals, 76°-78°C
The following compounds are prepared as described in Example 62.
PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl-phenyl]-2-chlorobenzami de, m.p. 116°-119°C PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)y1)carbonyl]-phenyl]-2,5-dichlorobe nzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl-phenyl]-2,4-dichloroben zamide, m.p. 112°-115°C PAC N-[4-[-(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-fluorobenza mide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-chloro-4-met hylbenzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-methyl-4-chl orobenzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazenin-10(11H)yl)carbonyl]-phenyl]-2,4-dimethylbe nzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2,3-dimethylbe nzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-methoxybenza mide PAC N-[4-[(Dibenz[b.f][1,41]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-(trifluorom ethoxy)benzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2,4-dimethoxyb enzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2,6-dimethoxyb enzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyyl-phenyl]-benzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2,6-dichlorobe nzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2,6-dimethylbe nzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-methylthiobe nzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-methyl-3-thi ophenecarboxamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-3-methyl-2-thi onhenecarboxamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-methyl-3-fur anecarboxamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-3-methyl-2-fur anecarboxamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-henylacetamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H yl)carbonyl]-phenyl]-2-chlorophenylacetamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-methylphenyl acetamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-thiopheneace tamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-furaneacetam ide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-phenyl]-2-methyl-3-thi opheneacetamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-3-chlorophenyl]-2-meth ylbenzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)carbonyl]-2-methylphenyl]-2-meth ylbenzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2-methylbenz amideAs described for Example 2, 1 mmol of 4-[(2-methylbenzoyl)amino]benzoyl chloride, 1 mmol of 5H-dibenz[b,d]azepine and 2 mmol of triethylamine are stirred at room temperature for 5 hours to give the product as a pale yellow solid.
PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2-methylbenz amideA mixture of 0.10 g of 5-(4-aminobenzoyl)-6,7-dihydro-5H-dibenz[b,d]azepine, 0.10 g of triethylamine in 1 ml of dichloromethane is stirred at room temperature for 6 hours. The mixture is diluted with 6 ml of ethyl acetate and the solution washed with 1N HCl, 1N NaOH, brine and dried (Na2 SO4). The solvent is removed and the solid purified by chromatography on thick layer silica gel plates with the solvent ethyl acetate-hexane (1:1) to give 90 mg of a pale yellow solid.
The following compounds are prepared as described in Example 92.
PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azenin-5-yl)-carbonyl]phenyl]-2-chlorobenz amide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyyl-2,5-dichloro benzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b.d]azeoin-5-yl)-carbonyl]phenyl]-2,4-dichloro benzamide PAC N-[4-[(6.7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2-fluorobenz amide PAC N-[4-[(6.7-Dihydro-5H-dibenz[b,d]azeyin-5-yl)-carbonyl]phenyl]-2-chloro-4-m ethylbenzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azenin-5-yl)-carbonyl]phenyl]-2-methyl-4-c hlorobenzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azerin-5-yl)-carbonyl]phenyl]-2,4-dimethyl benzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b.d]azepin-5-yl)-carbonyl]phenyl]-2,3-dimethyl benzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2-methoxyben zamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2-(trifluoro methoxy)benzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2,4-dimethox ybenzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2,6-dimethox ybenzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]benzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azenin-5-yl)-carbonyl]phenyl]-2,6-dichloro benzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2,6-dimethyl benzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2-methylthio benzamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2-methyl-3-t hiophenecarboxamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-3-methyl-2-t hiophenecarboxamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2-methyl-3-f uranecarboxamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-3-methyl-2-f uranecarboxamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]phenylacetami de PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2-chlorophen ylacetamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azenin-5-yl)-carbonyl]phenyl]-2-methylphen ylacetamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]thiophene-2-c arboxamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2-methyl-3-t hiopheneacetamide PAC N-[4-[(6,7-Dihydro-5H-dibenz[b,d]azepin-5-yl)-carbonyl]phenyl]-2-methyl-3-f uraneacetamide PAC N-[4-f(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-2-methyl-3- furanecarboxamideAs described for Example 9, 2-methyl-3-furanecarbonyl chloride is reacted with 5-(4-amino-benzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine to give the product. Recrystallization from dichloromethane-hexane gives crystals, m.p. 202°-204°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-3-chloroben zo [b]thiophene-2-carboxamideAs described for Example 9, 3-chlorobenzo[b]-thiophene-2-carbonyl chloride is reacted with 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine to give the product. Recrystallization from dichloro-methane-hexane gives crystals, m.p. 252°-254°C
As described for
A mixture of 0.314 g of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine and 0.173 g of o-tolylisocyanate in 15 ml of tetrahydrofuran is refluxed overnight. An additional 84 mg of o-tolylisocyanate is added and the mixture refluxed for three hours. The solyent is removed, water added to the residue and the residue extracted with dichloromethane. The extract is washed with 1N HCl, H2 O, 1M NaHCO3, brine and dried (Na2 SO4). The solution is filtered through a thin pad of hydrous magnesium silicate. The pad is washed with dichloromethane. The filtrate (140 ml) is discarded. The filter pad is washed with acetone to give 0.370 g of product. Trituration with dichloromethane-hexane gives 0.186 g of crystals, m.p. 188°-190°C
PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]-N-(2-methylphenyl)ben zamideTo a mixture of 0.362 g of 4-[(6,11-dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]benzoyl chloride and 0.101 g of triethylamine in 5 ml of dichloromethane is added a solution of 0.129 g of 2-methylaniline in 3 ml of dichloromethane. The mixture is stirred 1.5 hr at room temperature and then washed with H2 O 1N HCl 1M NAHCO3, brine and dried (Na2 SO4). The solvent is removed to give a solid. The solid is dissolved in dichloromethane and filtered through a thin pad of hydrous magnesium silicate with dichloromethane as eluent to give 0.025 g of crystals, m.p. 214°-216°C
PAC 4-[(6.11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]-N-(2,3-dimethylphenyl )benzamideAs described for Example 123, a mixture of 0.361 g of 4-[(6,11-dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]benzoyl chloride, 0.101 g of triethylamine and 0.145 g of 2,3-dimethylaniline is stirred for 1.5 hr. and worked up to give 0.44 g of crystals, m.p. 248°-251°C
The following compounds are prepared as described in Example 123.
PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-N-(2-chloropheny1) benzamide PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-N-(2,4-dichlorophenyl) benzamide PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-N-(2,5-dichlorophenyl) benzamide PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-N-(2,4-dimethylphenyl] benzamide PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-N-(2,5-dimethylphenyl) benzamide PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-N-(2-methoxyphenyl)ben zamide PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-N-(2,4-dimethoxyphenyl )benzamide PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-N-(3-chloro-4-methoxyp henyl)benzamide PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-N-(5-chloro-2-methoxyD henyl)benzamide PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-N-(3-chlorophenyl)benz amide PAC 4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]-N-(2-chloro-5-methoxyp henyl)benzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl-2-methylbenzamideA mixture of 0.349 g of 2-chloro-5-(4-amino-benzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.131 g of triethylamine and 0.201 g of 2-methylbenzoyl chloride in 13 ml of dichloromethane is stirred at room temperature for 3 hours. The mixture is poured into water and the organic layer separated. The organic layer is washed with 1N HCl, H2 O, 1N NaHCO3, brine and dried (Na2 SO4). The solution is filtered through a thin pad of hydrous magnesium silicate and the pad washed with dichloromethane. The filtrate is concentrated and the solid crystallized from dichloromethane-hexane to give 0.32 g of crystals, m.p. 187°-189°C
PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]2,4-dichlorobenzamideAs described for Example 136, a mixture of 0.349 g of 2-chloro-5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.131 g of triethylamine and 0.272 g of 2,4-dichlorobenzoyl chloride in 13 ml of dichloromethane is stirred at room temperature for 3 hours. Work-up gives a solid which is crystallized from dichloromethane-hexane to give 0.43 g of crystals, m.p. 199°-201°C
PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]2,3-dimethylbenzamideAs described for Example 136, a mixture of 0.349 g of 2-chloro-5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.131 g of triethylamine and 0.219 g of 2,3-dimethylbenzoyl chloride in 13 ml of dichloromethane is stirred at room temperature for 18 hours. Work-up gives a solid which is recrystallized from dichloromethane-hexane to give 0.38 g of crystals 191°-193°C
PAC 2-Chloro-6.11-dihydro-5-[4- [[[(2-methylphenyl)aminol-carbonyl]amino]benzoyl]-5H-dibenz[b,e]azepineAs described for Example 122, a mixture of 0.348 g of 2-chloro-5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine and 0.175 g of o-tolylisocyanate in 15 ml of tetrahydrofuran is refluxed overnight and worked-up to give the product as a solid.
The following compounds are prepared as described in Example 136.
PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-chlorobenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2,5-dichlorobenzamide PAC N-[4-f(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-chloro-4-methylbenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-methyl-4-chlorobenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2,4-dimethylbenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2,5-dimethylbenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-methoxybenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5- 5 yl)carbonyyl1henyl]-2-trifluoromethoxybenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]yhenyl]-2,4-dimethoxybenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2,6-dimethoxybenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbony]phenyl]-2,6-dichlorobenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyliphenyl]-2,6-dimethylbenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-methylthiobenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-methylthiophene-3-carboxamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-3-methylthioDhene-2-carboxamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-methylfurane-3-carboxamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-3-methylfurane-2-carboxamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-chlorophenylacetamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-methylphenylacetamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-methoxy-4-chlorobenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-trifluorobenzamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-methoxyphenylacetamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]cyclohexylcarboxamide PAC N-(4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-3-cyclohexenecarboxamide PAC N-[4-[(2-Chloro-6,11-dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]cyclohexylacetamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-2-methoxy-4-chlorobenzamideAs described for Example 9, a mixture of 0.377 g (1 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.295 g of 2-methoxy-4-chloro-benzoyl chloride, and 0.15 g of triethylamine in 10 ml of dichloromethane is stirred at room temperature for 3 hours. An additional 0.148 g of 2-methoxy-4-chloro-benzoyl chloride and 75 mg of triethylamine is added and the mixture stirred oyernight. The solution is worked-up as for Example 9 to give 0.38 g of product (crystallized from dichloromethane-hexane) m.p. 237°-239°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz(b,e]azepin-5-yl) carbonyl]phenyl]-2-trifluoromethylbenzamideAs described for Example 9, a mixture of 0.377 g (1.44 mmol) of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.15 g of triethylamine and 0.300 g (1.44 mmol) of 2-trifluoromethylbenzoyl chloride is stirred at room temperature for 2 hours and then washed with 1N HCl 1M NaHCO3, brine and dried (Na2 SO4). The solution is passed through a thin pad of hydrous magnesium silicate and the filtrate evaporated to give a solid. Crystallization from dichloromethane-hexane gives 0.41 g of crystals, m.p. 191°-193°C
PAC N-[4-[ (6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-methylbenzamideA mixture of 0.291 g of 6,11-dihydro-5H-di-benz[b,e]azepine, 0.518 g of 4-[N-methyl-N-(2-methyl-benzoyl)amino]benzoyl chloride and 0.182 g of triethylamine in 10 ml of tetrahydrofuran is stirred at room temperature for 2 hours. The solyent is removed, the residue diluted with water and extracted with dichloromethane. The extract is washed with 1 N HCl water, 1M NaHCO3, brine and dried (Na2 SO4). The solution is passed through a thin pad of hydrous magnesium silicate. The filtrate is concentrated and chilled to give 0.52 g of crystals, m.p. 160°-170°C
As described for Example 167, but substituting the appropriate aroyl chloride, the following compounds are prepared.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]- N-methyl-2-chlorobenzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2,5-dichlorobenzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2,4-dichlorobenzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-fluorobenzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-chloro-4-methylbenzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-methyl-4-chlorobenzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2,4-dimethylbenzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2,3-dimethylbenzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-methoxybenzamide PAC N-(4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-trifluoromethoxy-benzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2,4-dimethoxybenzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-methoxy-4-chloro-benzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-methylthiobenzamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-methylthionhene-3-carboxamide PAC N-[4-[(6,11-Dihydro-5B-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-3-methyl-2-thiophene-carboxamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl carbonyl]phenyl]-N-methyl-2-methyl-3-furane-carboxamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-3-methyl-2-furane-carboxamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methylphenylacetamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-chlorophenylacetamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-methoxyphenylacetamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-methyylhenylacetamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-methyl-3-thiophene-acetamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methyl-2-trifluoromethylacetamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methylcyclohexanecarboxamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl) carbonyl]phenyl]-N-methYl-3-cyclohexanecarboxamide PAC N-[4-[(5,6-Dihydro-7H-pyrimido[5,4-d][1]benzazepin-7-yl) carbonyl]phenyl]-2-methylbenzamideAs described for Example 1, 5 mmol of 4-[(2-methylbenzoyl)amino]benzoyl chloride is reacted with 5 mmol of 5,6-dihydro-7H-pyrimido[5,4-d][1]benz-azepine in pyridine to give the product as a solid.
PAC N-[4-[(5,6-Dihydro-7H-pyrimido[5,4-d][1]benzazepin-7-yl) carbonyl]phenyl]-2,4-dichlorobenzamideAs described for Example 1, 5 mmol of 4-[(2,4-dichlorobenzoyl)amino]benzoyl chloride is reacted with 5 mmol of 5,6-dihydro-7H-pyrimido[5,4-d]-[1]benzazepine in pyridine to give the product as a solid.
PAC N-[4-[(5,6-Dihydro-7H-pyrimido[5,4-d][1]benzazepin-7-yl) carbonyl]phenyl]-2,5-dichlorobenzamideAs described for Example 1, 5 mmol of 4-[(2,5-dichlorobenzoyl)amino]benzoyl chloride is reacted with 5 mmol of 5,6-dihydro-7H-pyrimido-[5,4-d][1]benzazepine in pyridine to give the product as a solid.
PAC N-[4-[(5,6-Dihydro-7H-pyrimido[5,4-d][1]benzazepin-7-yl) carbonyl]phenyl]-2-chlorobenzamideAs described for Example 1, 5 mmol of 4-[(2-chlorobenzoyl)amino]benzoyl chloride is reacted with 5 mmol of 5,6-dihydro-7H-pyrimido[5,4-d][1]benzazepine in pyridine to give the product as a solid.
PAC N-[4-[(5,6-Dihydro-7H-pyrimido[5,4-d][1]benzazepin-7-yl) carbonyl]phenyl]-2-chlorophenylacetamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-methylbenzamideA mixture of 5 mmol of 5,11-dihydro-6-(4-aminobenzoyl)-6H-pyrido[3,2-e][1]benzazepine, 5.5 mmol of 2-methylbenzoyl chloride and 10 mmol of triethylamine in 15 ml of dichloromethane is stirred at room temperature for 16 hours. The mixture is diluted with 50 ml of dichloromethane and solution washed with 20 ml each of H2 O, 1M citric acid, NaHCO3, brine and dried (Na2 SO4). The solvent is evaporated in vacuo to give a solid. The solid is purified by chromatography on silica gel to give the product as a solid.
The following compounds are prepared as described for in Example 198.
PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-chlorobenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2,5-dichlorobenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2,4-dichlorobenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-chloro-4-methylbenzamide PAC N-[4-[(5,11-Dihydro-6H--pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-methyl-4-chlorobenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e-][1]benzazepin-6-yl) carbonyl]phenyl]-2,4-dimethylbenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2,3-dimethylbenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-methoxybenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-trifluoromethoxybenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2,4-dimethoxybenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-methoxy-4-chlorobenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-trifluoromethylbenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-methylthiobenzamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-methyl-3-thiophenecarboxamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-3-methyl-2-thiophenecarboxamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-methyl-3-furanecarboxamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-3-methyl-2-furanecarboxamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-methoxyphenylacetamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-chlorophenylacetamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-methylphenylacetamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-2-methyl-3-thionheneacetamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]cyclohexanecarboxamide PAC N-[4-[(5,11-Dihydro-6H-pyrido[3,2-e][1]benzazepin-6-yl) carbonyl]phenyl]-3-cyclohexanecarboxamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-methylbenzamideA mixture of 5 mmol of 5,6-dihydro-5-(4-aminobenzoyl) pyrido[2,3-b][1,4]benzothiazepine, 5.5 mmol of 2-methylbenzoyl chloride and 10 mmol of triethylamine in 15 ml of dichloromethane is stirred at room temperature for 16 hours. The mixture is diluted with 50 ml of dichloromethane and the solution washed with 20 ml each of H2 O, 1M citric acid, NaHCO3, brine and dried (Na2 SO4). The solvent is removed under vacuum to give a solid. The solid is purified by chromatography on silica gel with ethyl acetate-hexane as solvent to give the product as a solid.
The following compounds are prepared as described for in Example 222.
PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-chlorobenzamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2,5-dichlorobenzamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2,4-dichlorobenzamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-chloro-4-methylbenzamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-methyl-4-chlorobenzamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2,4-dimethylbenzamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2,3-dimethylbenzamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-methoxybenzamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-trifluoromethoxybenzamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2,4-dimethoxybenzamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-methoxy-4-chlorobenzamide PAC N-[4-(Pyrido[2,3-b-][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-trifluoromethylbenzamide PAC N-[4-(Pyrido[2,3-b-][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-methylthiobenzamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-methyl-3-thiophenecarboxamide PAC N-[4-(Pyrido[2,3-b]l[1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-3-methyl-2-thiophenecarboxamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-methyl-3-furanecarboxamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-3-methyl-2-furanecarboxamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]phenylacetamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-chlorophenylacetamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-methoxyphenylacetamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-methylphenylacetamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-2-methyl-3-thiopheneacetamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]cyclohexanecarboxamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]cyclopentanecarboxamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]cyclohexaneacetamide PAC N-[4-(Pyrido[2,3-b][1,4]benzothiazepin-5(6H)-ylcarbonyl) phenyl]-3-cyclohexenecarboxamide PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-2,3 5-trichlorobenzamideA mixture of 366 mg of 2,3,5-trichlorobenzoyl chloride and 151 mg of triethylamine in 2 ml of methylene chloride is stirred while 314 mg of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine in 5 ml of methylene chloride is added. Stirring is continued for 1.5 hours. The reaction mixture is partitioned with water and the organic layer is washed with 1N HCl, water, 0.5 N NaOH and brine. The organic layer is dried with Na2 SO4 and passed through a pad of hydrous magnesium silicate. Hexane is added to the filtrate at the boil to give 0.52 g of white solid residue which is purified by column chromatography on silica gel by elution with 2:1 hexane-ethyl acetate to give 120 mg of the desired product as a crystalline solid, m.p. 225°-230°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-3,4-dichlor obenzamideA mixture of 314 mg of 3,4-dichlorobenzoyl chloride and 151 mg of triethylamine in 2 ml of methylene chloride is stirred while 314 mg of 5-(4-aninobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine in 5 ml of methylene chloride is added. Stirring is continued for 2 hours. The reaction mixture is partitioned with water and the organic layer is washed 1N HCl, water, 0.5N NaOH and brine. The organic layer is dried with Na2 SO4 and passed through a short pad of hydrous magnesium silicate. Hexane is added to the filtrate at the boil to give 0.35 g of off-white crystalline solid, m.p. 238°-241°C
PAC N-(4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-3,5-dichlor obenzamideA mixture of 301.6 mg of 3,5-dichlorobenzoyl chloride and 145 mg of triethylamine is stirred in 2 ml of methylene chloride while 314 mg of 5-(4-amino-benzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine in 5 ml of methylene chloride is added. The reaction mixture is stirred at room temperature for 2 hours and 100 mg of 3,5-dichlorobenzoyl chloride added. The reactants are heated at reflux for 36 hours. The cooled reaction mixture layer washed with 1N HCl, water, 1N NaOH, water and brine. The organic layer is dried with Na2 SO4 and passed through a short pad of hydrous magnesium silicate and hexane added to the filtrate at the boil to give 242 mg of the desired product as a crystalline solid, m.p. 263°-265°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-2,6-dichlor obenzamideA mixture of 335 mg of 2,6-dichlorobenzoyl chloride and 258 mg of N,N-diisopropylethylamine is stirred in 2 ml of xylene while 314 mg of 5-(4-amino-benzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine is added. The reactants are refluxed in an oil bath at 110°C for 18 hours. The xylene is eyaporated in vacuo to a residue which is partitioned between methylene chloride and water. The organic layer is separated, washed with 1N HCl, 1M NaHCO3, and brine. The organic layer is dried with Na2 SO4 and passed through a pad of hydrous magnesium silicate. Hexane is added to the filtrate at the boil to give 370 mg of the desired product as a crystalline solid, m.p. 257°-259°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-2,3-dichlor obenzamideA mixture of 301.6 mg of 2,3-dichlorobenzoyl chloride and 145 mg of triethylamine is stirred while 314 mg of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz-[b,e]azepine is added. Stirring is continued for 2 hours. Water is added and the organic layer washed with 1N HCl, water, 1M NaHCO3 and water then dried oyer Na2 SO4. The organic layer is passed through a pad of hydrous magnesium silicate and hexane added to the filtrate at the boil to give 0.32 g of the desired product as a crystalline solid, m.p. 225°-230°C
PAC N-(4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-2-methyl-3- fluorobenzamideA mixture of 248.5 mg of 1-methyl-2-fluoro-benzoyl chloride and 145 mg of triethylamine is stirred while 376.8 mg of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine is added. Stirring is continued for 3 hours. Water is added and the organic layer washed with 1N HCl, water, 1M NaHCO3 and water then dried oyer Na2 SO4. The organic layer is passed through a pad of hydrous magnesium silicate. The desired product crystallizes from methylene chloride to give 0.417 g of the desired product, m.p. 167°-170°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-3-cyclohexe ne-1-carboxamideA mixture of 208 mg of 3-cyclohexene-1-carbonyl chloride (prepared from 3-cyclohexene-1-carboxylic acid and thionyl chloride) and 145 mg of triethylamine in 3 ml of methylene chloride is added to a solution of 377 mg of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine in 7 ml of methylene chloride. The mixture is stirred for 18 hours, washed with water, 1N HCl, water, 1M NaHCO3 and brine then dried with Na2 SO4. The organic layer is passed through a short column of hydrous magnesium silicate and hexane added to the filtrate at the boil to give 340 mg of the desired product as a crystalline solid, m.p. 234°-236°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]-3-chlorophenyl]-2, 4-dichlorobenzamideA mixture of 0.50 g of 5-(2-chloro-4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.28 g of N,N-diisopropylethylamine and 0.45 g of 2,4-dichlorobenzoyl chloride in 25 ml of methylene chloride is stirred at room temperature for 18 hours. The reaction mixture is washed with water, saturated NaHCO3, dried (Na2 SO4) and passed through a short pad of hydrous magnesium silicate. The filtrate is evaporated in vacuo to give the desired product as a solid residue, m.p., 150°-165°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]-2-methoxyphenyl]-2 -methylbenzamideA mixture of 1.0 g of 5-(3-methoxy-4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.47 g of N,N-diisopropylethylamine and 0.56 g of 2-methylbenzoyl chloride in 25 ml of methylene chloride is stirred at room temperature for 18 hours. The reaction mixture is washed with water and saturated NaHCO3, dried (Na2 SO4) and passed through a short pad of hydrous magnesium silicate. Hexane is added at the boil to give 1.27 g of the desired product as a crystalline solid, m.p. 209°-210 °C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]-2,6-dimethylphenyl ]-2-methylbenzamideA mixture of 1.42 g of 4-[(benzoyl)amino]-3,5-dimethylbenzoic acid in 20 ml of thionyl chloride is heated on a steam bath under argon for 1 hour. The volatiles are evaporated and the residue evaporated in vacuo from toluene to give 1.40 g of the desired product as a residue which is dissolved in 50 ml of methylene chloride and treated with 0.75 g of N,N-diisopropylethylamine and 0.88 g of 10,11-dihydro-5H-dibenz[b,e]azepine. The reactants are stirred at room temperature for 18 hours and washed with water, saturated NaHCO3, dried (Na2 SO4), passed through a pad of hydrous magnesium silicate and hexane added to the filtrate at the boil to give 0.90 g of the desired product as a glass.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]-2,6-dimethylphenyl ]-2 4-dichlorobenzamideA mixture of 1.69 g of 4-[(2,4-dichlorobenzoyl) amino]-3,5-dimethylbenzoic acid in 2.0 ml of thionyl chloride is heated on a steam bath under argon for 1 hour. The volatiles are evaporated and the residue evaporated in vacuo from toluene to give a residue. A solution of the residue in 25 ml of methylene chloride is treated with 0.75 g of N,N-diisopropylethylamine and 0.98 g of 10,11-dihydro-5H-dibenz[b,e]azepine is stirred at room temperature for 18 hours. The reaction mixture is washed with saturated NaHCO3, dried (Na2 SO4) and passed through a pad of hydrous magnesium silicate. The filtrate is evaporated to a glass which is dissolved in methylene chloride dried (Na2 So4) and passed through a short pad of hydrous magnesium silicate. The filtrate is evaporated to give 1.89 g of the desired product as an amorphorous solid.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]-3-chlorophenyl]-2- methylbenzamideA mixture of 0.31 g of 5-(2-chloro-4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine and 0.15 g of N,N-diisopropylethylamine in 10 ml of methylene chloride is cooled in an ice bath while 0.18 g of 2-methylbenzoyl chloride is added. The bath is removed and the reactants stirred at room temperature for 18 hours. The mixture is washed with water, 1N HCl, water, 1M NaHCO3, brine and dried (Na2 SO4). The methylene chloride is removed in vacuo to give 0.34 g of the desired product as crystalline solid, m.p. 138°-150°C M+ =467.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)- carbonyl]-2-methoxyphenyl]-2-methyl-5-fluorobenzamideA mixture of 0.79 g of 5-(3-methoxy-4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.40 g of N,N-diisopropylethylamine and 0.55 g of 2-methyl-5-fluorobenzoyl chloride in 25 ml of methylene chloride is stirred at room temperature for 18 hours. The reaction mixture is washed with water and saturated NaHCO3, dried (Na2 SO4) and passed through a short pad of hydrous magnesium silicate. Hexane is added at the boil to the filtrate to give 1.05 g of the desired product as a solid.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]-2-chlorophenyl]-2- methyl-3-fluorobenzamideA mixture of 1.0 g of 6,11-dihydro-5H-dibenz-[b,e]azepine, 0.80 g of N,N-diisopropylethylamine and 2.01 g of 4-[(2-methyl-3-fluorobenzoyl)amino]-3-chlorobenzoyl chloride in 100 ml of methylene chloride is stirred at room temperature for 18 hours. The reaction mixture is washed with water, saturated NaHCO3, dried (Na2 SO4) and passed through a pad of hydrous magnesium silicate. Hexane is added to the filtrate at the boil to give 1.79 g of the desired product as a crystalline solid, m.p. 254°-256° C.
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]-2-methoxyphenyl]-2 ,4-dichlorobenzamideA mixture of 1.0 g of 5-(3-methoxy-4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.47 g of N,N-diisopropylethylamine and 0.76 g of 2,4-dichlorobenzoyl chloride in 25 ml of methylene chloride is stirred at room temperature for 18 hours. The reaction mixture is washed with water and saturated NaHCO3, dried (Na2 SO4) and passed through a short pad of hydrous magnesium silicate. Hexane is added at the boil to give 1.39 g of the desired product as a crystalline solid, m.p. 212°-214°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]-3-chlorophenyl]-2- methyl-3-fluorobenzamideA mixture of 1.85 g of 4-[(2-methyl-3-fluorobenzoyl)amino]-2-chlorobenzoic acid in 30 ml of thionyl chloride under argon is heated at reflux for 1 hour. The thionyl chloride is evaporated in vacuo to a residue which is stirred with hexane and collected to give 1.94 g of 4-[(2-methyl-3-fluorobenzoyl)amino]-2-chlorobenzoyl chloride which is dissolved in 25 ml of methylene chloride and 0.49 g of N,N-diisopropylethylamine added, followed by 0.65 g of 6,11-dihydro-5H-dibenz[b,e]azepine. The reactants are stirred at room temperature for 18 hours and washed with water, saturated NaHCO3, dried (Na2 SO4) and passed through a short pad of hydrous magnesium silicate to give 1.19 g of the desired product as a glass.
PAC N-[4-[(6,11-Dihydro-11-oxo-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-2-me thylbenzamideA mixture of 1.08 g of N-[4-[(6,11-dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-2-methylben zamide in 50 ml of t-butanol is heated, 2 ml of water is added followed by 1.18 g of MgSO4, and 2.6 g of KMnO4. An additional 0.84 g of KMnO4 in 25 ml of water is added followed by heating at 65° C for 18 hours. The reaction mixture is filtered and the filtrate evaporated in vacuo to give 1.2 g of a residue which is purified by column chromatography on silica gel by elution with ethyl acetate and 9:1 ethyl acetate-methanol to give 160 mg of the desired product as a solid, m.p. 115°-119°C
PAC (2-Methylphenyl)methyl 4-[(6,11-dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl)benzoateTo a mixture of 248 mg of 2-methylbenzyl alcohol in 4 ml of anhydrous tetrahydrofuran is added 80 mg of sodium hydride (60% in mineral oil) and the mixture stirred for 1 hour. To the mixture is added 430 mg of 4-[(6,11-dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]benzoyl chloride and the mixture is stirred for 18 hours. The tetrahydrofuran is evaporated in vacuo to a residue which is partitioned between methylene chloride and water. The organic layer is separated and washed with 1N HCl, water, 1M NaHCO3, and brine. The organic layer is dried (Na2 SO4) and passed through a short pad of hydrous magnesium silicate and hexane added at the boil to give 170 mg of the desired product, m.p. 140°-142°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-N-[(dimethy lamino)methyl]-2-methylbenzamideTo a suspension of 14 mg of 60% sodium hydride in oil, in 2 ml of tetrahydrofuran is added 0.13 g of N-[4-[(6,11-dihydro-5H-dibenz[b,e]azepin-5-yl)carbonyl]phenyl]-2-methylben zamide. The reactants are stirred for 1 hour and 62 mg of N,N-dimethylmethylene ammonium iodide added followed by stirring for 2 hours. The mixture is diluted with 10 ml of ether and filtered. The filtrate is evaporated in vacuo to a residue which is stirred with hexanes to give 0.13 g of the desired product as a white solid, m.p. 131°-133°C
PAC N-[4-[(5,11-dihydro-10H-dibenz[b,e](1,4]diazepin-5-yl)carbonyl]phenyl]-2-me thylbenzamideA mixture of 1.0 g of 5,11-dihydro-10-dibenz [b,e][1,4]diazepine 1.9 g of 4-[(2-methylbenzoyl) amino]benzoyl chloride, 4 ml of triethylamine and 0.2 g of 4-(dimethylamino)pyridine in 60 ml of methylene chloride is stirred at room temperature for 18 hours. The reaction mixture is poured into ice water and the separated organic layer washed with 50 ml each of water, 2N HC1, water, saturated NaHCO3 and water. The organic layer is dried (Na2 SO4) and the solvent removed to give a residue. The residue is chromatographed on a silica gel column using 1:4 ethyl acetatehexane to give 1.8 g of the desired product as a solid, m.p. 68°-71° C.
The following compounds are prepared as described for Example 268.
PAC N-[4-[(5,11- Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-chlorobenzami de PAC N-[4-[(5,11- Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2,4-dichloroben zamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2,5- dichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-3, 5-dichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-fl uorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-3-fl uorobenzamide PAC N-[4-[(5,11-Dihydro-10H--dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]1-2- methyl-4-chlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]1-2,3 -dimethylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-me thoxybenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-(t rifluoromethoxy)benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2,4- dimethoxybenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2,6- dimethoxybenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-me thoxy-4-chlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-(t rifluoromethyl)benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-3-(t rifluoromethyl)benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]l-2,6 -dichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-(m ethylthio)benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-4-fl uoro-2-(trifluoromethyl) benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-4-fl uoro-3-(trifluoromethyl) benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-fl uoro-3-(trifluoromethyl) benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-3,5- dimethylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2,3, 5-trichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2,5- difluorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-3-fl uoro-2-methylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]- 2,3-dichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-4-fl uoro-2-methylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-5-fl uoro-2-methylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-fl uoro-5-(trifluoromethyl) benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-fl uoro-6-(trifluoromethyl) benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]- 3-fluoro-5-(trifluoromethyl) benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-me thyl-3-thiophenecarboxamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-3-me thyl-2-thiophenecarboxamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-me thyl-3-furanecarboxamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-3-me thyl-2-furanecarboxamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]- 2-chlorobenzeneacetamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-me thylbenzeneacetamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-me thoxybenzeneacetamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-me thyl-3-thiopheneacetamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-3-me thyl-2-thiopheneacetamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]phenyl]-2-me thyl-3-furaneacetamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-2-methoxyph enyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-2-methoxyph enyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-2-methoxyph enyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl) carbonyl]-2-methoxyphenyl]-2,6-dichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-2-methoxyph enyl]-2-methylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-2-methophen yl]-2,5-dichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-2-methylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-2-chlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-2,3-dimethylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-2,3-dichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-2,4-dichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-4-fluoro-2-(trifluoromethyl)benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-2-(methylthio)benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-2-(trifluoromethoxy) benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-2,6-dichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-chlorophe nyl]-2-fluoro-6-(trifluoromethyl)benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl) carbonyl]-3-methylphenyl]-2,6-dichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-methylphe nyl]-2-fluoro-6-(trifluoromethyl)benzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-methylphe nyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-methylphe nyl]-2,4-dichlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-methylphe nyl]-4-fluoro-2-chlorobenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-methylphe nyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-methylphe nyl]-2,3-dimethylbenzamide PAC N-[4-[(5,11-Dihydro-10H-dibenz[b,e][1,4]diazepin-5-yl)carbonyl]-3-methylphe nyl]-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-2 -methylbenzamideA partial solution of 0.250 g of 5,6-dihydro-6-(4-aminobenzoyl)-4H-isoxazolo[4,5-d][1]benzazepine in 4 ml of tetrahydrofuran-dioxane (1:3) under argon is treated with 160 μl of 2-methylbenzoyl chloride in 1.5 ml of dioxane followed by 114 μl of triethylamine and stirring continued for 4.5 hours at room temperature. The volatiles are evaporated in vacuo to a residue which is dissolved in methylene chloride containing methanol, washed with 10H% NaHCO3 and brine, then treated with activated carbon. The mixture is filtered through MgSO4 and the filtrate filtered through silica gel with 15% ethylacetate in methylene chloride. The filtrate is evaporated in vacuo to a residue which is dissolved in methylene chloride, filtered through glass wool, evaporated to a residue which is dissolved in ethyl acetate by warming to give 0.25 g of the desired product as tan crystals, m.p. 257°-260°C HR FABMS: (M+H)=424.1657.
The following compounds are prepared as described in EXAMPLE 345.
PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-2 -methyl-4-chlorobenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]- 2,3-dimethylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-2 -methoxybenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-2 -(trifluoromethoxy)benzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-2 -(trifluoromethyl)benzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]l- 2-(methylthio)benzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-2 ,3-dichlorobenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-3 -fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-5 -fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-2 -chloro-4-fluorobenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-4 -fluoro-2-(trifluoromethyl) benzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]- 4-fluoro-3-(trifluoromethyl) benzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-4 -fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yI)carbonyl]phenyl]-2 -fluoro-5-(trifluoromethyl) benzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]phenyl]-2 -fluoro-6-(trifluoromethyl) benzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-chloro phenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-chloro phenyl]-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-chloro phenyl]-2,3-dimethylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-chloro phenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-chloro phenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-chloro phenyl]-2,3-dichlorobenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methyl phenyl]-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methyl phenyl]-2,3-dimethylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methyl phenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methyl phenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methyl phenyl]-4-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methyl phenyl]-2-methylthiobenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methyl phenyl]-2-(trifluoromethoxy)benzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methyl phenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methox yphenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methox yphenyl]-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methox yphenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(4,5-Dihydro-6H-isoxazolo[4,5-d][1]benzazepin-6-yl)carbonyl]-3-methox yphenyl]-2,3-dichlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-methylbenzamideTo a mixture of 0.420 g of 2,4,5,6-tetrahydro-2-methyl-6-(4-aminobenzoyl)pyrazolo[4,3-d][1]-benzazepi ne and 275 μl of triethylamine in 6 ml of methylene chloride and 2 ml of dioxane is added a solution of 215 μl of 2-methylbenzoyl chloride in 1.5 ml of dioxane. The reactants are stirred under argon for 4.5 hours. The volatiles are evaporated in vacuo to a residue which is dissolved in methylene chloride and washed with 10% NaHCO3 and brine. The organic layer is treated with activated carbon, dried with MgSO4 and evaporated an vacuo to give 660 mg of a tan foam residue. The residue is purified by column chromatography on silica gel by elution with 30% ethyl acetate-methylene chloride to give 590 mg of the desired product as white crystalline solid, m.p. 246°-248°C; HR FABMS: (M+H)=437.1972.
The following examples are prepared as described for Example 379.
PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-chlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2,4-dichlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2,3-dichlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-methyl-4-chlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2,3-dimethylbenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-methoxybenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-(trifluoromethoxy)benzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2,4-dimethoxybenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-methoxy-4-chlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-(trifluoromethyl)benzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-3-(trifluoromethyl)benzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-(methylthio)-benzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-4-fluoro-2-(trifluoromethyl)benzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-fluoro-3-(trifluoromethyl)benzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-3-fluoro-5-(trifluoromethyl)benzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-chloro-5-(methylthio)benzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-methyl-3-thiophenecarboxamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-methyl-3-furanecarboxamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-chlorobenzeneacetamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] phenyl]-2-methylbenzeneacetamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-chlorophenyl]-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-chlorophenyl]-2,3-dimethylbenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-chlorophenyl]-2,3-dichlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-chlorophenyl]-2,4-dichlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-chlorophenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-chlorophenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-chlorophenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-methylphenyl]-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-methylphenyl]-2,3-dimethylbenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-methylphenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-methylphenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-methylphenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methylpyrazolo[4,3-d][1]-benzazepin-6(2H)-yl)carbonyl] -3-methylphenyl]-2,4-dichlorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-2-methylbenzamideTo a solution of 0.216 mg of 6,7-dihydro-2-methyl-7-(4-aminobenzoyl)-5H-pyrimido[5,4-d][1]benzazepine in 6 ml of methylene chloride under argon is added 100 μl of triethylamine followed by a solution of 94 μl of 2-methylbenzoyl chloride in 1.5 ml of methylene chloride. The reaction mixture is stirred at room temperature for 18 hours, washed with water, saturated 5 NaHCO3 and the separated organic layer treated with activated carbon and filtered through MgSO4. The filtrate is evaporated to a residue which is dissolved in ethyl acetate and evaporated in vacuo to give 300 mg of the desired product as a pale yellow foam. HR FABMS: Exact Mass (M+H):449.1974.
The following compounds are prepared as described in Example 416.
PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-2,3-dimethylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-2,4-dichlorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-2,3-dichlorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-2-methoxybenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-2-(trifluoromethoxy)benzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-2-methoxy-4-chlorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-2-chloro-5-fluorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]- 2-(trifluoromethyl)-benzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-2-(methylthio)-benzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] phenyl]-fluoro-2-methylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-chlorophenyl]-2-methylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-chlorophenyl]-2,3-dimethylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-chlorophenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-chlorophenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-chlorophenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-chlorophenyl]-2-chloro-5-fluorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-chlorophenyl]-2,3-dichlorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-chlorophenyl]-2-(trifluoromethoxy)benzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-methoxyphenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-methoxyphenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-methylphenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-methylphenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-methylphenyl]-2,3-dichlorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-methylphenyl]-2-chloro-5-fluorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-methylphenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-methylphenyl]-2,3-dimethylbenzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-methylphenyl]-2-(trifluoromethyl)benzamide PAC N-[4-[(6,7-Dihydro-2-methyl-5H-pyrimido[5,4-d][1]-benzazepin-7-yl)carbonyl] -3-methylphenyl]-2-(trifluoromethoxy)benzamide PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)yl)-carbonyl]phenyl]-2-chlorobenzam ideTo a solution of 385 mg of 2-chloro-benzoyl chloride in 6 ml of methylene chloride at 0°C is added 0.6 g of 10-(4-aminobenzoyl)-10,11-dihydrodibenz[b,f]-[1,4]thiazepine followed by 0.375 ml of triethylamine. The reaction mixture is stirred at room temperature for 18 hours. The reaction mixture is washed with 20 ml of water, 1N HCl, 1N Na2 CO3, water and dried over MgSO4. The filtrate is evaporated in vacuo to give 0.7 g of yellowish solid which is purified by silica gel chromatography on thick layer plates by elution with 1:1 ethyl acetate-hexane to give 0.3 g of the desired product, m.p. 116°-119°C
PAC N-[4-[(Dibenz[b,f][1,4]thiazepin-10(11H)-yl)-carbonyl]phenyl]-2 4-dichlorobenzamideTo a solution of 452 mg of 2,4-dichlorobenzoyl chloride in 6 ml of methylene chloride is added 0.6 g of 10-(4-aminobenzoyl)-10,11-dihydrodibenz[b,f]-[1,4]thiazepine followed by 0.375 ml of triethylamine. The reaction mixture is stirred at room temperature for 18 hours. The reaction mixture is washed with 20 ml of water, 1N HCl, 1N Na2 CO3, water and dried over MgSO4. The filtrate is evaporated in vacuo to give 0.72 g of yellowish solid which is purified by silica gel chromatography on thick layer plates by elution with 1:1 ethyl acetate-hexane to give 0.254 g of the desired product as ivory crystals, m.p. 112°-115°C
PAC 5-[4-[[(2-Chlorophenyl)sulfonyl]amino]benzoyl]-6,11-dihydro-5H-dibenz [b,e]azepineA mixture of 0.13 g of 5-(4-aminobenzoyl)-6,11-dihydro-5H-dibenz[b,e]azepine, 0.21 g of 2-chlorobenzenesulfonyl chloride, 0.12 g of triethylamine and 5 mg of N,N-dimethylaminopyridine in 2 ml of methylene chloride is stirred at room temperature for 18 hours. The mixture is partitioned between 1N NaOH and ethyl acetate. The organic layer is washed with 50% NH4 Cl and brine, dried over Na2 SO4 and evaporated to give 0.26 g of the desired product as a yellow solid. MS(CI):663(M+H; C35).
A solution of 0.22 g of the preceding compound in 5 ml of tetrahydrofuran is treated with 2 ml of 1N NaOH and 2 ml of methanol and stirred at room temperature for 1 hour. The organic solvents are removed in vacuo and the residue diluted with 15 ml of ethyl acetate and 5 ml of water. The organic extract is washed with 5 ml of 1N NaOH, brine and dried (Na2 SO4) and evaporated in vacuo to give 0.19 g of yellow solid which is washed with diethyletherisopropyl ether to give 0.17 g of beige solid. MS(CI):489(M+H, Cl35).
PAC 6-[4-[(2,4-Dichlorobenzoyl)amino]benzoyl]-5,6-dihydropyrazolo[4,3-d][1]benz azepine-2(4H)-acetic acidTo a stirred slurry of 0.477 g of 6-[4-[(2,4-dichlorobenzoyl)amino]benzoyl]-5,6-dihydropyrazolo[4,3-d][1]ben zazepine-2(4lj)-acetic acid, ethyl ester in 15 ml of ethyl alcohol is added 5 ml of tetrahydrofuran followed by 1.30 ml of 1M NaOH. The reaction mixture is stirred at room temperature for 6 hours followed by the addition of 0.750 ml of 2M HCl. The acidic reaction mixture is evaporated in vacuo, triturated with CHCl3, combined, treated with activated carbon, filtered through MgSO4 and evaporated in vacuo to give 0.380 mg of the desired product as a clear foam.
PAC Ethyl 6-[4-[(2,4-dichlorobenzoyl)amino]benzoyl]-5,6-dihydropyrazolo[4,3-d][1]ben zazepine-2(4H)-acetateTo a stirred solution of 0.940 g of ethyl 6-(4-aminobenzoyl)-5,6-dihydropyrazolo[4,3-d][1]benzazepine-2(4H)-acetate in 25 ml of methylene chloride is added 369 μl of triethylamine followed by the dropwise addition of 373 μl of 2,4-dichlorobenzoyl chloride in 3.5 ml of methylene chloride. The reaction mixture is stirred at room temperature for 18 hours, washed with water, saturated NaHCO3, brine and the organic layer treated with activated carbon, and filtered through is MgSO4. The filtrate is concentrated in vacuo to a residue which is purified by flash chromatography on silica gel using 25% ethyl acetate in methylene chloride to give 380 mg of the desired product as white crystals, m.p. 164°-167°C The mother liquors are combined, evaporated in vacuo to a residue which is dissolved in 25% ethyl acetate in chloroform to give 535 mg of off-white solid, m.p. 160°-163.5°C
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]-2-(2-methyl propoxy)benzamideTo a mixture of 0.62 g of 2-chloro-5-nitrobenzoic acid and 0.61 g of triethylamine in 5 ml of methylene chloride is added 0.42 g of isobutylchloroformate at 0°C The mixture is warmed to room temperature for 30 minutes. A solution of 0.31 g of 5-(4-aminobenzoyl)-6,7-dihydro-5H-dibenz[b,d]azepine in 1 ml of methylene chloride is added followed by 10 mg of N,N-dimethylaminopyridine and 1.0 ml of toluene is added. The mixture is heated at 100°C for 48 hours. The room temperature reaction mixture is diluted with 15 ml of ethyl acetate and washed with 1N HCl, 1N NaOH, brine and dried (Na2 SO4) and evaporated in vacuo to a residue. The residue is purified by column chromatography on silica gel by elution with 1:2 ethyl acetate-hexane to give 0.28 g of the desired product as a yellow solid. MS(CI):536(M+H).
PAC N-[4-[(6,11-Dihydro-5H-dibenz[b,e]azepin-5-yl)-carbonyl]phenyl]- 2-(dimethylamino)acetamideTo a solution of 0.31 g of 5-(4-aminobenzoyl)-6,7-dihydro-5H-dibenz[b,e]azepine in 5 ml of methylene chloride is added 0.53 g of Na2 CO3 followed by 0.31 g of N,N-dimethylglycyl chloride. The mixture is stirred at room temperature for 20 hours. The mixture is quenched with water, extracted with ethyl acetate and the organic layer dried (Na2 SO4) and concentrated in vacuo to give 0.39 g of yellow foam. The yellow foam is treated with 15 ml of hydrochloric acid and the suspension washed with ethyl acetate. The aqueous suspension is made alkaline with 5N NaOH and extracted with 30 ml of ethyl acetate. The organic layer is washed with brine, dried (Na2 SO4) and evaporated to give 0.32 g of the desired product as an off-white solid. MS(CI): 400(M+H). A sample is treated with anhydrous HCl to give the hydrochloride salt. MS(CI): 400(M+H--HCl).
PAC N-[4-(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl)carbonyl1p henyl]-2,4-dichlorobenzamideTo a stirred solution of 0.235 g of 5,6-dihydro-2-methyl-6-(4-aminobenzoyl)-4H-thiazolo[5,4-d]-[1]benzazepine in 6 ml of methylene chloride under argon is added 107 μl of triethylamine followed by the dropwise addition of 109 μl of 2,4-dichlorobenzoyl chloride in 1 ml of methylene chloride. Stirring is continued at room temperature for 18 hours. The reaction mixture is washed with H2 O, saturated NaHCO3 and brine. The organic layer is treated with activated carbon, filtered through MgSo4 and the filtrate evaporated in vacuo to a residue which is chromatographed on silica gel by elution with 15% ethyl acetate in methylene chloride to give 330 mg of the desired product as a tan glass.
PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl)carbonyl]phenyl]-2-m ethylbenzamideA mixture of 400 mg of 4,10-dihydro-5H-thieno[3,2-c][1]benzazepine and 700 mg of 4-[(2-methylbenzoyl)amino]benzoyl chloride is stirred in 30 ml of methylene chloride in the presence of 3 ml of triethylamine for 8 hours. The volatiles are removed in vacuo to give a residue which is partitioned between chloroform and water. The organic layer is dried (Na2SO4) and the filtrate evaporated in vacuo to give a residue which is purified by column chromatography on silica gel by elution with 30% ethyl acetate-hexane to give 543 mg of the desired product. M+1=439.
PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl)carbonyl]phenyl]-2,3 -dichlorobenzamideA mixture of 200 mg of 9,10-dihydro-4H-thieno[2,3-c][1] and 350 mg of 4-[(2-methylbenzoyl)-amino]benzoyl chloride is stirred in 30 ml of methylene chloride in the presence of 2 ml of triethylamine for 8 hours. The volatiles are removed in vacuo to give a residue which is partitioned between water and chloroform. The organic layer is dried (Na2 So4) and the filtrate evaporated in vacuo to give a residue which is purified by column chromatography on silica gel by elution with 30% ethyl acetate-hexane to give 266 mg of the desired product. M+1=494.
PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonylvphenyl1-2-methylbenzamideA mixture of 400 mg of 9,10-dihydro-4H-thieno [2,3-c][1]benzazepine and 600 mg of 4-[(2-methylbenzoyl) amino]benzoyl chloride is stirred in 30 ml of methylene chloride in the presence of 3 ml of triethylamine for 8 hours. The volatiles are removed in vacuo to give a residue which is partitioned between water and chloroform. The organic layer is dried (Na2 So4) and the filtrate evaporated in vacuo to give a residue which is purified by column chromatography on silica gel by elution with 30% ethyl acetate-hexane to give 518 mg of the desired product. M+1=439.
The following examples are prepared using conditions of Example 456.
PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2,3-dimethylbenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2,5-dimethylbenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2-methoxybenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2-(trifluoromethoxy)-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2-methoxy-4-chlorobenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2-(methylthio)-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2,3-dichlorobenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2-methyl-3-thiophene-carboxamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-3-methyl-2-thiophene-carboxamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2-chlorobenzeneacetamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2,3-difluorobenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-4-fluoro-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2,3,5-trichlorobenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2-fluoro-5-(trifluoro-methyl)benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2-fluoro-6-(trifluoro-methyl)benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-3-fluoro-5-(trifluoro-methyl)benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2,6-dichlorobenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2-(trifluoromethyl)-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2-chloro-4-fluoro-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-chlorophenyl]-2-chloro-5-fluoro-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-fluorophenyl]-3-fluoro-2-methyl-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-fluorophenyl]-5-fluoro-2-methyl-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-fluorophenyl]-2-chloro-4-fluoro-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-methylphenyl]-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-methylphenyl]-3-fluoro-2-methyl-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-methylphenyl]-5-fluoro-2-methyl-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-methylphenyl]-2-chloro-4-fluoro-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-methylphenyl]-2-chloro-5-fluoro-benzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-methylphenyl]-2,3-dichlorobenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) 5 yl)carbonyl]-3-methylphenyl1-2-chloro-4-fluorobenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-methylphenyl]-2,3-dimethylbenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-methylphenyl]-2,4-dichlorobenzamide PAC N-[4-[(4,10-Dihydro-5H-thieno[3,2-c][1]benzazepin-5-yl) carbonyl]-3-methylphenyl]-2-(methylthio)-benzamideThe following examples are prepared using conditions of Example 458.
PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-chlorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2,3-dichlorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2,3-dimethylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2,4-dichlorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-methoxybenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-(trifluoromethoxy)-benzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-methoxy)-4-chlorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2,6-dichlorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-(trifluoromethyl)-benzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-(methylthio)-benzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-3-methyl-2-thiophene-carboxamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chloronhenyl]-2-methyl-3-thiophene-carboxamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-methylbenzeneacetamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-(trifluoromethyl)-4-fluorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2,5-dimethylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-chloro-5-fluorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-2-fluoro-5-(trifluoro-methyl)benzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-chlorophenyl]-3-fluoro-5-(trifluoro-methyl)benzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-fluorophenyl]-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-fluorophenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-fluorophenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-2,3-dimethylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-2,5-dimethylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-2,3-dichlorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-2,4-dichlorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-2-chloro-5-fluorobenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-2-fluoro-5-(trifluoro-methyl) benzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][libenzazepin-9-yl) carbonyl]-3-methylphenyl]-2-methyl-4-chloro-benzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-2-methoxybenzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-2-(trifluoromethoxv)-benzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyl]-2-(trifluoromethyl)-benzamide PAC N-[4-[(4,10-Dihydro-9H-thieno[2,3-c][1]benzazepin-9-yl) carbonyl]-3-methylphenyll-2-(thiomethyl)-benzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1-benzazeyin-6-yl) carbonyl]phenyl]-5-fluoro-2-methylbenzamideTo a stirred solution of 0.235 g of 5,6-di- hydro-2-methyl-6-(4-aminobenzoyl)4H-thiazolo[5,4-d][1]-benzazepine in 6 ml of methylene chloride under argon is added 107 μl of triethylamine followed by the drop wise addition of 2-methyl-5-fluorobenzoyl chloride in 1 ml of methylene chloride. Stirring is continued at room temperature for 18 hours. The reaction mixture is washed with H2 O, saturated NaHCO3 and brine. The organic layer is treated with activated carbon, filtered through MgSO4 and the filtrate evaporated in vacuo to a residue which is chromatographed on silica gel by elution with 15% ethyl acetate in methylene chloride to give 300 mg of the desired product as a white solid; Anal. Calc'd for C27 H22 FN3 O2 S: C,68.8; H,4.7; N,8.9; F,4.0; S,6.8 Found: C,67.7; H,4.6; N,8.5; F,3.7; S,6.4.
The following examples are prepared using the conditions of Example 534 with the appropriately substituted aroyl chloride.
PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-2,3-dichlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-2-methyl-4-chlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-2-methoxybenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-2-methoxy-4-chlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-2-(trifluoromethoxy)benzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d](l]-benzazepin-6-yl) carbonyl]-3-chloroohenyll-2-(trifluoromethyl)benzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][l1-benzazepin-6-yl) carbonyl]-3-chlorophenyl1-2,6-dichlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H--thiazolo(5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl1-2,3-dimethylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl1-2,5-dimethylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chloropheny11-3-(trifluoromethyl)benzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chloropheny1l-2-(methylthio)benzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-2-methyl-3-thiophenecarboxamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d]C1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-3-methyl-2-thiophenecarboxamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]- benzazenin-6-yl) carbonyl]-3-chlorophenyl]-3-methyl-2-furanecarboxamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d-]Cl]-benzazepin-6-yl) carbonyl]-3-chlorophenyl1-2-methylbenzeneacetamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chloroDhenyll-3-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl1-4-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-2-fluoro-4-(trifluoromethyl)benzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-3-fluoro-5-(trifluoromethyl benzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo(5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-chlorophenyl]-2-chloro-5-fluorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazenin-6-yl) carbonyl]-3-chlorophenyl]-4-fluoro-2-(trifluoromethyl)benzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][l1]-benzazepin-6-yl) carbonyl]-3-fluorophenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-fluorophenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-fluorophenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-fluorophenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-v1) carbonyl]-3-methylphenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methylphenyl]-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methylphenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methylphenyl]-2-chloro-5-fluorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methylphenyl]-2,3-dimethylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazeDin-6-yl) carbonyl]-3-methylphenyl]-2,5-dimethylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methylphenyl]-2,4-dichlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methylthenyl]-2-methoxy-4-chlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methylphenyl]-2-(trifluoromethoxvybenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methylphenyl]-2-(methylthio)benzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methoxyphenyl]-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methoxyphenyl]-2,4-dichlorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methoxyphenyl]-2-chloro-4-fluorobenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methoxyphenyl]-3-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methoxyphenyl]-5-fluoro-2-methylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methoxyphenyl]-2,3-dimethylbenzamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methoxyphenyl]-2-methylbenzeneacetamide PAC N-[4-[(4,5-Dihydro-2-methyl-6H-thiazolo[5,4-d][1]-benzazepin-6-yl) carbonyl]-3-methoxyphenyl]-2-chlorobenzeneacetamide PAC 6,11-Dihydro-5-[4-[(3-methylbutanoyl)amino]benzoyl]-5H-dibenz [b,e]azepineTo a stirred solution of 0.16 g of 6,11-di-hydro-5-(4-aminobenzoyl)-5H-dibenz [b,e]azepine in 2 ml of methylene chloride is added 0.10 g of triethylamine followed by 0.09 g of iso-valeryl chloride. After stirring at room temperature for 2 hours, the reaction mixture is evaporated in vacuo to a residue. The residue is extracted with ethyl acetate-methylene chloride, washed with brine, dried (Na2 SO4), filtered through hydrous magnesium silicate and evaporated in vacuo to a residue which is stirred with ether-hexanes to give 0.21 g of light yellow solid.
MS(CI): 399(M+H).
PAC N-[4-r(4,5-Dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl) carbonyl]-3-chlorophenyl]-5-fluoro-2-methylbenzamide(a) A solution of 42.6 g (0.211 mol) of 2-chloro-4-nitrobenzoic acid is reacted with 420 ml of thionyl chloride (refluxed for 3 hours and the excess thionyl chloride removed). Toluene is added several times and the solvent removed. The residue is dissolved in 400 ml of CH2 Cl2 and added dropwise to a cold (ice bath) solution of 28.2 g (0.192 mol) of 1,2,3,4-tetra-hydro-5H-1-benzazepine and 29.4 ml of triethylamine in 320 ml of dichloromethane. The mixture is stirred at room temperature overnight and is washed with H2 O, 10% HCl and 10% NaHCO3. Activated carbon is added to the organic layer and the mixture is filtered through a layer of MgSO4. The solvent is removed under vacuum and the residue chromatographed on silica gel (in two batches) with ethyl acetate-CH2 Cl2 (5:95) as eluent. The fractions containing product are combined and the solvent removed. The residue is dissolved in ethyl acetate and the solvent removed (foams). The residue is heated with hexane to give a solid (lumps broken up) and the mixture is filtered. The solid is washed with hexane and dried to give 59.9 g of 1,2,3,4-tetrahydro-1-(2-chloro-4-nitrobenzoyl)-5H-l-benzazepine as off-white crystals, m.p. 112°-114°C, mass spectrum (CI) 321 (MH+)
(b) To a hot (50°C) stirred solution of the preceding compound (15.0 g; 45.5 mmol) in 1 liter of tert-butanol is added 40 ml of water, 30.2 g (0.252 mol) of anhydrous MgSO4 and a solution of 15.0 g (95.6 mmol) of KMnO4 in 450 ml of water. The mixture is stirred and heated at 70°C overnight. The hot mixture is filtered through diatomaceous earth and the filter cake washed with hot tert-butanol. The filtrate is concentrated to dryness. To the residue is added water and the mixture extracted with CHCl13. The extracts are combined, dried (MgSO4) and the solvent removed to give a brown foam. The foam is chromatographed on silica gel with hexane-ethyl acetate (3:1) on a Waters HPLC apparatus to give crystals which are recrystallized from ethyl acetate-hexane to give 3.6 g of 1,2,3,4-tetrahydro-1-(2-chloro-4-nitrobenzoyl)-5H-1-benzazepin-5-one as crystals, m.p. 140°-143°C; mass spectrum (CI) 345 (MH+).
(c) A mixture of the preceding compound (3.50 g; 10.2 mmol) and 35 ml of tert-butoxybis(dimethyl-amino)methane (Bredereck's Reagent) is heated on a steam bath for 10 minutes. The mixture is concentrated to dryness under high vacuum. The residue is dissolved in ethyl acetate --CH2 Cl2 (1:1) and the solution filtered through a pad of silica gel. The filtrate is concentrated to dryness under vacuum to give 3.88 g of orange crystals; mass spectrum (CI) 400 (MH+).
(d) A solution of 3.64 g (9.12 mmol) of the preceding compound and 0.887 ml (18.2 mmol) of hydrazine hydrate in 115 ml of methanol is refluxed for 2 hours under argon. The solvent is removed and the residue dissolved in ethyl acetate --CH2 Cl2 (1:4) and filtered through a short column of silica gel. The fractions containing the product are combined and the solvent removed to give 3.10 g of 1,4,5,6-tetrahydro-6-(2-chloro-4-nitrobenzoyl) pyrazolo[4,3-d][1]benzazepine as a yellow foam; mass spectrum (CI) 369 (MH+).
(e) A solution of 1.20 g (3.26 mmol) of the preceding compound and 3.68 g (16.3 mmol) of SnCl2. H2 O in 60 ml of ethanol is refluxed under argon for 1 hour. To the cooled mixture is added ice water and 10% NaHCO3 until the pH is 8. The mixture is stirred for 4.5 hours and extracted with CHCl3. The combined extracts are dried (MgSO4) and the solvent removed. The residue is dissolved in 1.2 liters of ethyl acetate --CH2 Cl2 (6:4) and the solution filtered through a short column of silica gel. The fractions containing product are combined and the solvent removed to give 1.1 g of 1,4,5,6-tetrahydro-6-(2-chloro-4-aminobenzoyl)pyrazolo-[4,3-d][1]benzazepi ne as a yellow solid; mass spectrum (CI) 339 (MH+).
(f) To a solution of 0.40 g (1.18 mmol) of the preceding compound and 0.298 g (2.95 mmol) of triethylamine in 12 ml of tetrahydrofuran is added dropwise a solution of 0.507 g (2.95 mmol) of 5-fluoro-2-methylbenzoyl chloride in 4 ml of CH2 Cl2 -tetrahydrofuran (1:1). The mixture is stirred at room temperature overnight and the solvent removed under vacuum. To the residue is added CHCl3 and the mixture washed with H2 O, 10% NaHCO3, brine and dried (MgSO4). The solvent is removed and the residue dissolved in 7 ml of CH3 OH and 3.5 ml of tetrahydrofuran and 1.8 ml of 1M NaOH added. The solution is stirred for 3 hours, acidified with 2M HCl and concentrated under vacuum. The residue is diluted with H2 O and extracted with CHCl3. The combined extracts are washed with H2 O, 10% NaHCO3 and dried (Na2 SO4). The solvent is removed and the residue dissolved in ethyl acetate --CH2 Cl2 (4:6) and the solution filtered through silica gel. The filtrate is concentrated under vacuum and the solid triturated with ethyl acetate to give 0.47 g of the product of the the Example as white crystals, m.p. 279°-280°C; mass spectrum (high resolution-FAB) 475.1326 (M+H).
PAC N-[4-[(4,5-Dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl]-3-chlorophenyl]-3- fluoro-2-methylbenzamideTo a solution of 0.40 g (1.18 mmol) of 1,4,5,6-tetrahydro-6-(2-chloro-4-aminobenzoyl)pyrazolo- [4,3-d][llbenzazepine and 0.298 g (2.95 mmol) of triethylamine in 12 ml of tetrahydrofuran --CH2 Cl2 (1:1) is added dropwise a solution of 0.507 g (2.95 mmol) of 2-methyl-5-fluorobenzoyl chloride in 4 ml of tetrahydrofuran --CH2 Cl2 (1:1). The mixture is stirred overnight and the solvent removed. To the residue is added CHCl3 and the mixture washed with H2 O, 10% NaHCO3, brine and dried (MgSO4). The solvent is removed and the residue dissolved in a mixture of 7 ml of CH3 OH, 3.5 ml of tetrahydrofuran and 1.8 ml of 1M NaOH. The solution is stirred at room temperature for 3 hours, acidified with 2M HCl and concentrated under vacuum. To the residue is added CHCl3 and the mixture washed with H2 O, 10% NaHCO3 and dried (MgSO4). The solvent is removed and the residue dissolved in ethyl acetate --CH2 Cl2 (4:6) and the solution filtered through a pad of silica gel.
The filtrate is concentrated under vacuum to give 0.55 g of the product of the Example as an off-white foam; mass spectrum (high resolution-FAB) 475.1339 (M+H).
PAC N-[4-[(4,5-Dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl)carbonyl ]phenyl]-2,4-dichlorobenzamideTo a mixture of (0.535 g) of 1,4,5,6-tetrahydro-6-(4-aminobenzoyl) pyrazolo[4,3-d][1]benzazepine, 0.373 g of triethylamine in 15 ml of tetrahydrofuran --CH2 Cl2 (1:1) is added dropwise 0.777 g of 2,4-dichlorobenzoyl chloride in 4 ml of tetrahydrofuran --CH2 Cl2 (1:1). The mixture is stirred at room temperature overnight and the solvent removed. To the residue is added CHCl3 and the mixture washed with H2 O, 10% NaHCO3, brine and dried (MgSo4). The solvent is removed under vacuum to give 1.37 g of a tan foam. This foam is dissolved in 15 ml of tetrahydrofuran --CH3 OH (5:10) and 2.64 ml of 1M NaOH is added. The solution is stirred at room temperature for 2 hours, acidified with 2M HCl and the solvent removed. The residue is partitioned between H2 O and CHCl3 and the organic layer separated and washed with H2 O, 10% NaHCO3 and dried (MgSo4). The solvent is removed and the residue dissolved in ethyl acetate --CH2 Cl2 (4:6). The solution is filtered through silica gel and the filtrate concentrated under vacuum to give 0.75 g of product as a cream solid; mass spectrum (high resolution-FAB) 477.0891 (M+H).
PAC N-[4-[(4.5-Dihydropyrazolo[4,3-d][1]benzazepin-6(2H)-yl) carbonyl]phenyl]-5-fluoro-2-methylbenzamideTo a mixture of 0.535 g of 1,4,5,6-tetrahydro-6-(4-aminobenzoyl) pyrazolo[4,3-d][1]benzazepine, 0.373 g of triethylamine in 15 ml of tetrahydrofuran --CH2 Cl2 (1:1) is added dropwise 0.636 g of 5-fluoro-2-methylbenzoyl chloride in 4 ml of tetrahydrofuran --CH2 Cl2 (1:1). The mixture is stirred at room temperature overnight and the solvent removed. The residue is dissolved in CHCl3 and the solution washed with H2 O, 10% NaHCO3, brine and dried (MgSO4). The solvent is removed to give 1.17 g of a tan foam. This foam is dissolved in a mixture of 10 ml of methanol, 5 ml of tetrahydrofuran and 2.65 ml of 1M NaOH. The solution is stirred for 2.75 hours, acidified with 2M HCl and the solvent removed. The residue is partitioned between H2 O and CHCl3, the organic layer separated and washed with 10% NaHCO3 and dried (MgSO4). The filtrate is concentrated under vacuum to give 0.76 g of product as a tan foam; mass spectrum (high resilution-FAB) 441.1721 (M+H).
PAC N-[4-[(4.5-Dihydropyrazolo[4.3-d][1]benzaze-in-6(2H)-yl) carbonyl]phenyl]-2-methylbenzamideTo a mixture of 0.535 g of 1,4,5,6-tetrahydro-6-(4-aminobenzoyl) pyrazolo[4,3-d][1]benzazepine, 0.373 g of triethylamine in 15 ml of tetrahydrofuran --CH2 Cl2 is added dropwise 0.574 g of 2-methylbenzoyl chloride in 4 ml of tetrahydrofuran --CH2 C2 (1:1). The mixture is stirred at room temperature overnight, washed with brine and dried (MgSO4). The solvent is removed and the residue dissolved in ethyl acetate --CH2 Cl2 (1:4). The solution is filtered through silica gel and the filtrate concentrated to dryness. The residue is dissolved in a mixture of 5 ml of dioxane, 5 ml of methanol and 2.7 ml of 1M NaOH added. The solution is stirred at room temperature for 2.75 hours and acidified with 2M HCl The solvent is removed, water added and the mixture extracted with CH2 C2 --CH3 OH (9:1). The combined extracts are washed with H2 O and the aqueous layer extracted with CHCl3. The organic layers are combined, dried (MgSO4) and the solvent removed. The residue is dissolved in ethyl acetate --CH2 Cl2 (1:1) and the solution filtered through silica gel. The filtrate is concentrated under vacuum to give 0.59 g of the product of the
Albright, Jay Donald, Reich, Marvin Fred
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