The present invention discloses materials and methods useful to treat sensitivity of endothelially-compromised vascular smooth muscle. In one embodiment, CLC3 blockers, particularly compounds of the Formula I are used to treat sensitivity.
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1. A method to normalize the contractile response of vasculature in response to a vasoconstrictor agonist in a patient in need of such normalization, the vasculature comprising a vascular smooth muscle cell layer and a compromised endothelial cell layer, wherein the method comprises administering a pharmaceutically effective amount of a CLC3 blocker, or a pharmaceutically acceptable salt thereof, and wherein the vasoconstrictor agonist is norepinephrine.
2. A method of
##STR00009##
wherein
either R4 is H or a lower alkyl radical and R5 is a lower alkyl radical, or R4 and R5 are joined together with the adjacent nitrogen atom to form a heterocyclic radical;
R6 is H or a lower alkyl radical;
R7 is H, halo, OH, a lower alkyl radical, or is a buta-1,3-dienyl radical which together with the adjacent benzene ring forms a naphthyl radical;
R8 is H or OH; and
n is 2;
or a pharmaceutically acceptable salt thereof.
3. A method of
8. A method of
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This application claims priority to Provisional Patent Application 60/121,727 filed Feb. 26, 1999.
The present invention relates generally to the field of chemical agents affecting the vasculature. In particular, this invention contributes to the study of the affects of chemical agents in diseased, mechanically disrupted or genetically abnormal vasculature.
Vascular smooth muscle (herein referred to as “VSM”) in the normal state is covered by an endothelial layer of cells as indicated in
Chloride ion channels are present in VSM (Klockner 424 Pflugers Arch 231 (1991); Lamb et al., 75 Circ Res 742 (1994)) and have been shown to be activated by vasoconstrictor agonists (Klockner and Isenberg, 418 Pflugers Arch 168 1991); Pacaud et al., 97 Br J Pharmacol 139 (1989)). Chloride ion currents have also been shown to contribute functionally to norepinephrine-induced contraction of normal vasculature. Lamb and Barna, 275 Am J Physiol H151 (1998). In that study, tamoxifen was shown to have no effect on the norepinephrine-induced contraction of normal vasculature (vasculature with intact endothelium). In Lamb and Barna, 275 Am J Physiol H161 (1998), the endothelium was shown to modulate the contribution of the chloride currents to norepinephrine-induced VSM constriction. The effects of tamoxifen on endothelium-compromised tissue was not studied, since no effect was seen in normal tissues. In other studies, a particular chloride ion channel, “CLC3”, was shown to be responsible for swelling-induced chloride conductance. Duan et al., 390 Nature 417 (1997). Tamoxifen was shown to block the ion channel responsible for swelling-induced chloride conductance, a result which had previously been demonstrated. Nilius et al., 428 Pflugers Arch 364 (1994).
The effects of tamoxifen on estrogen levels, and concomitant effects on other systems, including NE and dopamine expression, have also been studied. Kocsis et al., Vol. 69, Br J Exp Path 157 (1988); Etgen and Petitti, 49(6) J Neurochem 1732 (1987); Baksi et al., 20 Neuropharm 1163 (1981). Moreover, a method for treating peripheral vasoconstriction with tamoxifen citrate has been disclosed in U.S. Pat. No. 5,470,883. In that patent, the anti-estrogen effects of tamoxifen were said to be responsible for reducing the peripheral vasoconstriction of exogenously-administered adrenergic compound.
Vascular smooth muscle can be damaged by mechanical or physiological means. Medical procedures, such as balloon angioplasty, disease-induced or genetically-influenced pathogies, such as diabetes and hypertension, create risk or predisposal for endothelial damage. In addition, endothelial damage in itself may exacerbate these pathologic processes and contribute to symptoms which are associated with them. For instance, coronary artery disease results in localized endothelial damage, and sudden surges in natural vasoconstrictors (such as NE) can cause heart failure. Previous treatments for these endothelially-compromised patients have been limited to chemicals agents which cause system-wide VSM relaxation, and consequently, frequently cause side effects such as orthostasis (dizziness) due to transient low blood pressure during certain activities.
In general, the present invention provides methods to reduce the sensitivity of endothelially-compromised vascular smooth muscle. In certain embodiments, methods are herein provided to influence blood pressure, in a tissue-selective manner, by administering a ClC3 blocker. In contrast to previous agents which cause patients to have system-wide vascular smooth muscle relaxation, it is now possible to affect only the pathologic vascular smooth muscle. In other words, use of CLC3 blockers allows for tissue-targeting in a highly specific and reliable manner, which results in avoidance of the unpleasant side effects of system-wide vasodilation associated with previously-known treatments.
The present invention provides methods to reduce the sensitivity of endothelially-compromised vascular smooth muscle in a patient in need of such reduction, comprising administering a pharmaceutically effective amount of a CLC3 blocker. Preferred are methods to reduce the sensitivity of endothelially-compromised vascular smooth muscle, comprising administering a compound of Formula I
##STR00001##
wherein
or a pharmaceutically acceptable salt thereof.
A most preferred embodiment of these methods are those wherein the compound administered is 1-p-β-dimethylaminoethoxyphenyl-trans-1,2-diphenylbut-1-ene (tamoxifen), or a pharmaceutically acceptable salt thereof.
Tamoxifen has the chemical structure:
##STR00002##
Also provided are methods to ameliorate the negative effects associated with vascular smooth muscle endothelium damage in a patient is need of such treatment, comprising administering a pharmaceutically-effective amount of a CLC3 blocker, or a pharmaceutically acceptable salt thereof. Preferred are methods as described, comprising administering a pharmaceutically effective amount of a compound of Formula I
##STR00003##
wherein
or a pharmaceutically acceptable salt thereof.
Methods as described, wherein said endothelium damage is the result of diabetes, surgical procedure, coronary artery disease or hypertension are preferred. Methods which further comprise administering a pharmaceutically-effective compound selected from the group consisting of: an anti-hypertension agent; an anti-diabetes agent; and anti-coronary artery disease agent; and an anti-restenosis agent are also preferred.
In another embodiment, there are provided methods to affect chloride ion channel 3 receptors comprising administering a compound of Formula I
##STR00004##
wherein
or a pharmaceutically acceptable salt thereof.
Preferred are methods as described, wherein the compound administered is 1-p-β-dimethylaminoethoxyphenyl-trans-1,2-diphenylbut-1-ene (tamoxifen), or a pharmaceutically acceptable salt thereof.
In yet another embodiment, there are provided methods to reduce contraction of endothelially-compromised vascular smooth muscle in response to a vasoconstrictive agent, comprising administering a chloride ion channel 3 blocker, or a salt thereof. A method as described, wherein the chloride ion channel 3 blocker is a compound of Formula I
##STR00005##
wherein
or a pharmaceutically acceptable salt thereof, is preferred.
Most preferred are methods as described, wherein the compound administered is 1-p-β-dimethylaminoethoxyphenyl-trans-1,2-diphenylbut-1-ene (tamoxifen), or a pharmaceutically acceptable salt thereof. Methods as described, wherein the agonist is NE, are preferred.
Also provided are methods to decrease the effects of vasoconstrictors in pathologic tissues and not in non-pathologic tissues in a patient with pathologic tissues, and who is in need of such decrease, comprising administering a pharmaceutically-effective amount of a CLC3 blocker, or a pharmaceutically acceptable salt thereof. Methods as described, wherein the CLC3 blocker is a compound of Formula I, wherein the CLC3 blocker is a compound of Formula I
##STR00006##
wherein
or a pharmaceutically acceptable salt thereof, are preferred.
Moreover, methods as described, wherein the compound administered is 1-p-β-dimethylaminoethoxyphenyl-trans-1,2-diphenylbut-1-ene (tamoxifen), or a pharmaceutically acceptable salt thereof, is more preferred.
In another embodiment of the present invention, there are provided methods to stabilize blood pressure in patients with endothelium-compromised vascular smooth muscle, and who are in need of such stabilization, comprising administering a pharmaceutically-effective amount of a CLC3 blocker. Methods wherein the CLC3 blocker is a compound of Formula I
##STR00007##
wherein
or a pharmaceutically acceptable salt thereof, are preferred.
Methods as described, wherein the CLC3 blocker is a compound of Formula I, or a pharmaceutically acceptable salt thereof, are preferred. More preferred are methods wherein the compound administered is 1-p-β-dimethylaminoethoxyphenyl-trans-1,2-diphenylbut-1-ene (tamoxifen), or a pharmaceutically acceptable salt thereof.
Definitions:
“a” or “an”, when describing a noun, refers to one or more of that noun.
“agent” means any compound or composition made by any means. “Agent” includes synthetic or naturally-occurring compounds or compositions, whether purified or not, and can include: herbal extract(s); precursor(s); metabolite(s); and ingredient(s), including enantiomer(s) of a racemic mixture. The definition of “agent” includes any compound or composition as described in this paragraph, which has been shown to be active for the desired medical purpose, including any agent which works to affect a desired medical result, and/or is approved by the US Food & Drug Administration, or foreign equivalent.
“anti-coronary artery disease agent” means any agent which causes reduction in the effects of coronary artery disease, and/or which is considered by the medical or scientific community, or the general public, to reduce coronary artery disease or the symptoms associated with coronary artery disease.
“anti-diabetes agent” means any agent which causes reduction in diabetes, and/or which is considered by the medical or scientific community, or the general public, to reduce diabetes or the symptoms associated with diabetes.
“anti-hypertension agent” means any agent which causes reduction in hypertension, and/or which is considered by the medical or scientific community, or the general public, to reduce hypertension or the symptoms associated with hypertension.
“anti-restenosis” means any agent which causes reduction in restenosis, and/or which is considered by the medical or scientific community, or the general public, to reduce restenosis or the symptoms associated with restenosis.
“CLC3” means chloride ion channel 3, and has the same meaning as CLC-3.
“damage” means any reduction in physiological or structural function, whether caused by mechanical, chemical or other means. The standard for determining whether function is “reduced” is determined by comparing the state of being in question to either population normals or individual normals. Moreover, if CLC3 blockers, in particular, tamoxifen or tamoxifen analogues are able to decrease vascular sensitivity to agonists in the patient, “damage” is assumed.
“effective amount” means that dosage of active compound(s) sufficient to provide therapeutic treatment of the specified medical indication.
“patient” means any living organism with vascular smooth muscle.
“surgical procedure” means any medical procedure requiring mechanical or mechanical/chemical manipulation of a patient's body, wherein said procedure results in damage to the endothelium layer adjoining vascular smooth muscle.
The present invention provides methods to reduce the sensitivity of endothelially-compromised vascular smooth muscle in a patient in need of such reduction, comprising administering a pharmaceutically effective amount of a CLC3 blocker, or a pharmaceutically-acceptable salt thereof. Methods as described, wherein the CLC3 blocker is a compound of Formula I
##STR00008##
wherein
or a pharmaceutically acceptable salt thereof, are preferred.
A more preferred embodiment of these methods are those wherein the compound administered is 1-p-β-dimethylaminoethoxyphenyl-trans-1,2-diphenylbut-1-ene(tamoxifen), or a pharmaceutically acceptable salt thereof.
Also provided are methods to ameliorate the negative effects associated with vascular smooth muscle endothelium damage in a patient is need of such amelioration, comprising administering a pharmaceutically effective amount of a CLC3 blocker, or a pharmaceutically acceptable salt thereof. Methods as described, wherein the CLC3 blocker is a compound of Formula I, are preferred. More preferred are methods as described, wherein the compound administered is tamoxifen, or a pharmaceutically-acceptable salt thereof. Methods as described, wherein said endothelium damage is the result of diabetes, surgical procedure, coronary artery disease, or hypertension are also preferred. Methods which further comprises administering a pharmaceutically-effective compound selected from the group consisting of: an anti-hypertension agent; an anti-diabetes agent; an anti-coronary artery disease agent; and an anti-restenosis agent are also preferred.
In another embodiment, there are provided methods to affect CLC3 receptors comprising administering a compound of Formula I, or a pharmaceutically acceptable salt thereof. Preferred are methods as described, wherein the compound administered is 1-p-β-dimethylaminoethoxyphenyl-trans-1,2-diphenylbut-1-ene (tamoxifen), or a pharmaceutically acceptable salt thereof.
In yet another embodiment, there are provided methods to reduce contraction of endothelially-compromised vascular smooth muscle in response to a vasoconstrictive agent, comprising administering a CLC3 blocker, or a salt thereof. Methods as described, wherein the CLC3 blocker is a compound of Formula I, or a pharmaceutically acceptable salt thereof are preferred. More preferred are methods as described, wherein the compound administered is 1-p-β-dimethylaminoethoxyphenyl-trans-1,2-diphenylbut-1-ene (tamoxifen), or a pharmaceutically acceptable salt thereof. Methods as described, wherein the agonist is NE, are preferred.
Also provided are methods to decrease the effects of vasoconstrictors in pathologic tissues and not in non-pathologic tissues, in a patient with pathologic tissues and who is in need of such decrease, comprising administering a pharmaceutically-effective amount of a CLC3 blocker, or a pharmaceutically acceptable salt thereof. Methods as described, wherein the CLC3 blocker is a compound of Formula I, or a pharmaceutically acceptable salt thereof, are preferred.
Moreover, methods as described, wherein the compound administered is 1-p-β-dimethylaminoethoxyphenyl-trans-1,2-diphenylbut-1-ene (tamoxifen), or a pharmaceutically acceptable salt thereof, is more preferred.
In another embodiment of the present invention, there are provided methods to stabilize blood pressure in patients with endothelium-compromised vascular smooth muscle, and who are in need of such stabilization, comprising administering a pharmaceutically-effective amount of a CLC3 blocker, or a pharmaceutically acceptable salt thereof. Methods as described, wherein the CLC3 blocker is a compound of Formula I, or a pharmaceutically acceptable salt thereof, are preferred. More preferred are methods wherein the compound administered is 1-p-β-dimethylaminoethoxyphenyl-trans-1,2-diphenylbut-1-ene (tamoxifen), or a pharmaceutically acceptable salt thereof.
The descriptive chemical terms used with Formula I have their usual meaning. For example, the term “halo” includes bromo, chloro, fluoro, and iodo. The term “lower alkyl” or “C1–C4 alkyl” refers to the straight and branched aliphatic radicals of 1–4 carbon atoms including methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, sec-butyl, and tert-butyl. In addition, the term “substituted phenyl” refers to a phenyl molecule having one or two substituents selected from the group consisting of C1–C4 alkyl, C1–C5 alkoxy, hydroxy, nitro, chloro, fluoro, or tri(chloro or fluoro)methyl. Finally, the term “C1–C4 alkoxy” includes the straight and branched aliphatic ether radicals of 1–4 carbon atoms such as methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, isobutoxy, sec-butoxy, and tert-butoxy.
A preferred Formula I compound, in which R4 and R5 each are methyl, R6 is ethyl, R7 and R8 each are H, and n is 2, is known in the art as tamoxifen. Tamoxifen and its analogs are most well known as antiestrogen compounds and tamoxifen primarily is used for the treatment of breast carcinoma in women. See, The Merk Index, 11th Ed., 1430 (1989). Tamoxifen citrate (Nolvadex.®., Zeneca Pharmaceuticals, Wilmington, Del. 19897) is a trans-isomer of a triphenylethylene derivative. Tamoxifen citrate has a molecular weight of 563.62, the pKa is 8.85, the equilibrium solubility in water at 37 degrees C. is 0.5 mg/mL, and in 0.002N. HCl at 37 degrees C., it is 0.2 mg/mL.
For therapeutic treatment of the specified indications, compounds of the present composition, particularly a Formula I compound, can be compounded and formulated into pharmaceutical compositions in unit dosage form for parenteral, transdermal, rectal, nasal, intravenous administration or, preferably, oral administration. Such pharmaceutical compositions are prepared in a manner well known in the art and comprise a Formula I compound, optionally including an additional compound. In making the compositions of the present invention, the active ingredients will usually be mixed with a carrier, or diluted by a carrier, or enclosed within a carrier which may be in the form of a capsule, sachet, paper or other container. When the carrier serves as a diluent, it may be a solid, semisolid, or liquid material which acts as a vehicle, excipient or medium for the active ingredient. Thus, the composition can be in the form of tablets, pills, powders, lozenges, sachets, cachets, elixirs, emulsions, solutions, syrups, suspensions, soft and hard gelatin capsules, sterile injectable solutions, and sterile packaged powders.
Additionally, compounds of the present composition, particularly Formula I compounds, are well suited to formulation as sustained release dosage forms and the like. The formulations can be so construed that they release the active ingredient only or preferably in a particular physiological location, possibly over a period of time. The coatings, envelopes, and protective matrices may be made, for example, from polymeric substances or waxes.
Some examples of suitable carriers, excipients, and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, starches, gum acacia, calcium phosphate alginates, calcium salicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, tragacanth, gelatin, syrup, methyl cellulose, methyl- and propylhydroxybenzoates, talc, magnesium stearate, water, and mineral oil. The compositions can additionally include lubricating agents, wetting agents, emulsifying and suspending agents, preserving agents, sweetening agents or flavoring agents. The compositions may be formulated so as to provide quick, sustained, or delayed release of the active ingredient(s) after administration to the patient by employing procedures well known in the art. For oral administration, a compound optionally including a second component compound, can be admixed with carriers and diluents molded into tablets or enclosed in gelatin capsules. The mixtures can alternatively be dissolved in liquids such as 10% aqueous glucose solution, isotonic saline, sterile water, or the like, and administered intravenously or by injection.
The compositions are preferably formulated in a unit dosage form, each dosage containing from about 1 to about 500 mg and, more frequently, from about 5 to about 300 mg of the active ingredient(s). The term “unit dosage form” refers to physically discreet units suitable as unitary dosages for human subjects, each unit containing a predetermined quantity of active ingredients calculated to produce the desired therapeutic effect, in association with the required pharmaceutically acceptable carrier. By “pharmaceutically acceptable”, it is meant the carrier, diluent, or excipient must be acceptable with the other ingredients of the formulation and not deleterious to the recipient thereof.
Compounds of the present invention, can be formulated alone or in combination with another pharmaceutical agent, and can generally will be administered in a convenient formulation. The following formulation examples only are illustrative and are not intended to limit the scope of the present invention.
Formulations
Ingredient
Quantity (mg/capsule)
Formulation 1: Gelatin Capsules
Hard gelatin capsules are prepared using the
following:
Formula I compound
0.1–1000
Starch, NF
0–650
Starch flowable powder
0–650
Silicone fluid 350 centistokes
0–15
Formulation 2: Formula I capsule
Formula I compound
1
Starch, NF
112
Starch flowable powder
225.3
Silicone fluid 350 centistokes
1.7
Formulation 3: Formula I capsule
Formula I compound
5
Starch, NF
108
Starch flowable powder
225.3
Silicone fluid 350 centistokes
1.7
Formulation 4: Formula I capsule
Formula I compound
10
Starch, NF
103
Starch flowable powder
225.3
Silicone fluid 350 centistokes
1.7
Formulation 5: Formula I capsule
Formula I compound
50
Starch, NF
150
Starch flowable powder
397
Silicone fluid 350 centistokes
3.0
The specific formulations above may be changed in compliance with the reasonable variations provided, and can include other pharmaceutically-active agents, such as those used to treat hypertension, diabetes, coronary artery disease and restenosis.
A tablet formulation is prepared using the ingredients below:
Formulation 6: Tablets
Ingredient
Quantity (mg/tablet)
Formula I compound
2.5–1000
Cellulose, microcrystalline
200–650
Silicon dioxide, fumed
10–650
Stearate acid
5–15
The components are blended and compressed to form tablets.
Alternatively, tablets each containing 25–1000 mg of a formula I compound are made up as follows:
Formulation 7: Tablets
Ingredient
Quantity (mg/tablet)
Formula I compound
25–1000
Starch
45
Cellulose, microcrystalline
35
Polyvinylpyrrolidone
4
(as 10% solution in water)
Sodium carboxymethyl cellulose
4.5
Magnesium stearate
0.5
Talc
1
The Formula I compound, starch, and cellulose are passed through a No. 45 mesh U.S. sieve and mixed thoroughly. The solution of polyvinylpyrrolidone is mixed with the resultant powders which are then passed through a No. 14 mesh U.S. sieve. The granules so produced are dried at 500.degree.-60.degree. C. and passed through a No. 18 mesh U.S. sieve. The sodium carboxymethyl starch, magnesium stearate, and talc, previously passed through a No. 60 U.S. sieve, are then added to the granules which, after mixing, are compressed on a tablet machine to yield tablets.
Suspensions each containing 25–1000 mg of medicament per 5 ml dose are made as follows:
Formulation 8: Suspensions
Quantity
Ingredient
(mg/5 ml)
Formula I compound
25–1000
mg
Sodium carboxymethyl cellulose
50
mg
Syrup
1.25
mg
Benzoic acid solution
0.10
mL
Flavor
q.v.
Color
q.v.
Purified water to
5
mL
The medicament is passed through a No. 45 mesh U.S. sieve and mixed with the sodium carboxymethyl cellulose and syrup to form a smooth paste. The benzoic acid solution, flavor, and color are diluted with some of the water and added, with stirring. Sufficient water is then added to produce the required volume.
Diagnosis of hypertension, diabetes, restenosis, coronary artery disease or other endothelium-compromised vascular smooth muscle states is within skill of the art, and is evidenced by the internal medicine textbook, such as Harrison's.
Determination whether a compound is a CLC3 blocker can be accomplished according to the Examples herein described.
The compounds can be purchased from commercial sources, such as Sigma Chemical, St. Louis, Mo. However, one skilled in the art is aware how to synthesize these compounds de novo using conventional methods. In particular, compounds of Formula I can be prepared according to the procedures described in U.S. Pat. No. 4,623,600, which patent is hereby incorporated by reference. Pharmaceutically acid addition salts can be prepared according to the disclosure in U.S. Pat. No. 5,691,355, which patent is hereby incorporated by reference.
In studies by the present inventors, it was discovered that DIDS (4,4′-diisothiocyanato stilbene-2,2′disulphonic acid) and niflumic acid significantly inhibited the contractile response to ED80 concentrations of norepinephrine (NE) or potassium chloride (KCl) in isolated rings of rat aorta with an intact endothelium. Significantly, tamoxifen did not alter these responses. These vasodilator effects of DIDS and niflumic acid (in the presence of NE or KCl) were endothelium-independent, because they persisted in epithelium-denuded blood vessel segments. The effect of tamoxifen on denuded blood vessel segments was not studied, since no effect was apparent in intact vessels. The above experiments were reported in Lamb and Barna, 275 Am J Physiol H151 (1998) and Lamb and Barna, 275 Am J Physiol H161 (1998), and are incorporated herein by reference.
Adult male Sprague-Dawley rats (250–300 g) were obtained from Harlan Sprague Dawley. The animals were killed by exposure to 100% CO2 for 5 minutes, followed by cervical dislocation. Thoracic aortas were removed, cleaned of adherent connective tissue, and cut into 6 mm rings. The endothelium was left intact, and the rings were mounted in individual 10-ml isolated organ chambers using standard methods for recording of isometric tension. Contractile responses were recorded with an eight-channel MacLab8E and stored on a Power Macintosh 7200 computer. passive stretch was set at 2.5 g, and the rings were allowed to equilibrate in physiological salt solution (PSS) at 37° C. for 120 minutes before the start of experimentation. PSS was aerated with a mixture of 95% O2-5% CO2; the composition was as follows (in mM): 130 NaCl, 4.7 KCl, 1.18 KH2PO4, 1.17 MgSO4.7H2O, 14.9 NaHCO3, 1.6 CaCl2H2O, 5.5 dextrose, and 0.03 CaNa2-EDTA 0.03 (pH 7.30).
The blood vessel segments were pretreated, for 30 minutes, either with a 1:1000 dilution of ethanol, or with 10 μM tamoxifen (in ethanol, from Sigma Chemical, St. Louis, Mo.).
Sensitivity was quantified by measuring the 50% effective dose of agonist (ED50) by linear regression following log transformation of the agonist concentration and logit transformation of the response data. Data were expressed as a percentage of the maximal response of each ring to the agonist.
Electrophysiology. Chloride ion currents were measured from cultured human aortic and coronary vascular smooth muscle cells at room temperature (22–24 C) using standard whole-cell voltage-clamp techniques (Hamill et al., 1981; Lamb et al., 1994) and an Axopatch 200B patch-clamp amplifier driven by pClamp software (Axon Instruments). The hypotonic (250 mOsm/kg by freezing point depression) bath solution contained (mM): NaCl 125, MgCl2 2.5, CaCl2 2.5, HEPES 10, Ph 7.2. Isotonic bath solution had the same ionic composition as the hypotonic solution except that the osmolality was adjusted to 300 mOsm/kg by adding mannitol. Pipette solution contained (mM): N′-methyl-D-glucamine chloride (NMDG-Cl) 135, EGTA 2, Mg-ATP 5, HEPES 10, pH 7.2 with osmolality adjusted to 300 mOsm/kg by adding mannitol. Thus, the chloride equilibrium potential remained 0 mV under both isotonic and hypotonic conditions. All components of the buffer solutions were obtained from Sigma. Pipette resistances were 3–5 MOhms.
Patch-clamp recording of the swelling-induced Cl ion current from cultured vascular smooth muscle cells revealed that this current is inhibited by tamoxifen, using methods described above. In contrast, β-estradiol (E, 10−7 M) did not inhibit but rather increased the swelling-induced Cl current (ICI, pA) seen under hypotonic conditions at +120 mV (hypotonic only=1451±495 pA, hypotonic plus estradiol=3513±856, n=5). This result suggests that the ability of Tamoxifen to inhibit this current cannot be accounted for by tamoxifen-induced activation of estrogen receptors.
The methods as described in Example 4 were used in this example as well. The protein kinase C (PKC) inhibitor H-7 (10−5 M) increased the swelling-induced chloride current above the level induced by hypotonic buffer alone. However, tamoxifen still inhibited the swelling-induced current in the presence of H-7 (hypotonic=1204±313 pA, hypotonic+H7+tamoxifen=878±493 pA, n=3). This result suggests that activation of PKC is not the mechanism by which tamoxifen inhibits the swelling-induced chloride current.
| Patent | Priority | Assignee | Title |
| Patent | Priority | Assignee | Title |
| 5077601, | Sep 09 1988 | Hitachi, LTD | Cooling system for cooling an electronic device and heat radiation fin for use in the cooling system |
| 5224538, | Nov 01 1991 | Dimpled heat transfer surface and method of making same | |
| 5470883, | May 23 1994 | Method of treating peripheral vasoconstriction with tamoxifen citrate | |
| 5472985, | May 13 1993 | UAB Research Foundation, The; Boston Scientific Scimed, Inc | Prevention and treatment of pathologies associated with abnormally proliferative smooth muscle cells |
| 5658936, | Sep 18 1995 | BRIGHAM & WOMEN S HOSPITAL, INC | Enhancement of erectile function with renin-angiotensin system inhibitors |
| 5691355, | May 05 1994 | Eli Lilly and Company | Method for minimizing the uterotrophic effect of tamoxifen and tamoxifen analogs |
| 5760066, | Apr 21 1995 | Sugen, Inc. | Compounds and methods for inhibiting hyper-proliferative cell growth |
| 5770609, | Jan 28 1993 | UAB Research Foundation, The; Boston Scientific Scimed, Inc | Prevention and treatment of cardiovascular pathologies |
| 5795898, | Jan 14 1994 | Cell Therapeutics, Inc. | Method for treating diseases mediated by cellular proliferation in response to PDGF,EGF,FGF and VEGF |
| 5811447, | Jan 28 1993 | UAB Research Foundation, The; Boston Scientific Scimed, Inc | Therapeutic inhibitor of vascular smooth muscle cells |
| 5912805, | Nov 04 1998 | Henkel Loctite Corporation | Thermal interface with adhesive |
| 5940269, | Feb 10 1998 | D-Link Corporation | Heat sink assembly for an electronic device |
| 5957194, | Jun 27 1996 | ADVANCED THERMAL SOLUTIONS, INC | Plate fin heat exchanger having fluid control means |
| 6015008, | Jul 14 1997 | Mitsubishi Electric Home Appliance Co., Ltd.; Mitsubishi Denki Kabushiki Kaisha | Heat radiating plate |
| 6054198, | Apr 29 1996 | Parker Intangibles LLC | Conformal thermal interface material for electronic components |
| 6197789, | Jun 07 1995 | UAB Research Foundation, The; Boston Scientific Scimed, Inc | Prevention and treatment of cardiovascular pathologies with tamoxifen analogues |
| WO9640098, |
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| Mar 09 2000 | LAMB, FRED S | UNIVERSITY OF IOWA RESEARCH FOUNDATION, INC | ASSIGNMENT OF ASSIGNORS INTEREST SEE DOCUMENT FOR DETAILS | 010960 | /0803 |
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