‘Mandarin Queen’ is a new and distinctive mandarin hybrid cultivar, which distinguishes itself by having mid to late season maturity, a large size, distinguished flat shape, easily peeled, a smooth rind texture, white albedo, red color, rich sweet flavor and pleasant aroma. It further distinguishes itself by being seedless or virtually seedless.
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1. A new and distinct cultivar of mandarin tree having the characteristics substantially as described and illustrated herein.
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Latin name of the genus and species: The mandarin cultivar of this invention is botanically identified as Citrus reticulata.
Variety denomination: The variety denomination is ‘Mandarin Queen’.
This invention relates to a new and distinctive mandarin hybrid cultivar designated ‘Queen’ which was developed in Spain. It is distinguished from other cultivars by producing fruit that combines mid to late season maturity, large size, distinguished flat shape, easily peeled, smooth rind texture, white albedo, red color, rich sweet flavor and pleasant aroma. It further distinguishes itself by being seedless or virtually seedless.
‘Mandarin Queen’ mandarin appeared in Pilar de la Horadada, Alicante, Spain in 1987 as a natural hybrid of a ‘Satsuma’ mandarin seed parent and an unknown pollen parent. First, seeds of the ‘Satsuma’ fruit were planted and one of the planted seeds produced a tree having different fruit. The tree from this planted seed was then grafted over ‘Macrophylla’, ‘Volkameriana’, ‘Carrizo’ and ‘Cleopatra’ rootstocks. Fruit production and evaluation began in 1995. Trials with cross pollination have been made with ‘Orogrande’, ‘Oronules’, ‘Ortanique’, ‘Clemenpons,’ ‘Fortune’ and ‘Valencia-Late’. Only the trials with ‘Fortune’ have occasionally produced some seed in the fruit of the ‘Mandarin Queen’ mandarin.
In 2006, budwood was sent to a center in Valencia, Spain, to clean the variety of the tristeza virus. The budwood was grafted to rootstock in Alicante, Spain, thereby asexually reproducing the new variety. In 2007, four trees certified as tristeza-free were kept in a breeding block of the center for further propagation and four trees were sent to a breeding block in Pilar de la Horadada, Alicante, Spain. Fruit production of those trees commenced in 2009.
The figures depict various characteristics of ‘Mandarin Queen’.
The following is a detailed description of the new Citrus reticulata, ‘Mandarin Queen’, based upon observation of four year old plants grown in Alicante, Spain. The color designations being according to The R.H.S. Colour Chart published by The Royal Horticultural Society of London, England. Further, additional character descriptions listed herein are in accordance with the IPGRI Descriptors for Citrus.
Fruit of ‘Queen’ mandarins are virtually seedless and do not cross-pollinate with other mandarins as it is auto incompatible. ‘Queen’ mandarin is a mid to late season variety, harvested between the beginning of January to the end of April in Pilar de la Horadada, Alicante, Spain. The fruit is very easy to peel, with a flat shape and having a diameter between 58-68 mm. The fruit has around 49% juice content, is very homogeneous, and has a red fruit skin color (see
The flavor is sweet and lightly acidic. The pulp has a smooth texture and the segments have an easy-eating texture. Some ‘Queen’ fruit have navels that differ from 1 to 5 mm in diameter and develop inside as a typical navel orange does (See
The ‘Mandarin Queen’ mandarin variety differs from its parents in harvesting period. ‘Satsuma’ is harvested in October through December and ‘Mandarin Queen’ is harvested in January through April, in Pilar de la Horadada, Alicante, Spain. ‘Satsuma’ has a puffy skin, while ‘Mandarin Queen’ has a skin that is easy to peel, but not puffy. ‘Satsuma’ has an orange color, while ‘Mandarin Queen’ has a vivid reddish orange color. ‘Satsuma’ has low level acidity during harvesting period, between 0.90 and 0.60, while ‘Mandarin Queen’ has an acid level of 1.25 and 1.00 during harvesting.
‘Mandarin Queen’ mandarin differs from main mid to late season cultivars because trees of ‘Mandarin Queen’ do not show alternate bearing and do not need to be pruned as, for example, ‘Orri’ or ‘Nadorcott’. ‘Mandarin Queen’ is virtually seedless, larger sized and a deeper red color than ‘Orri’ and ‘Nadorcott’. ‘Mandarin Queen’ mandarin's resistance to heat and cold is similar to ‘Clementine’.
The ‘Mandarin Queen’ mandarin tree has a drooping growth habit, obloid shape and is without spines. The bark of the trunk has a green and brown color (RHS color: 199 A) and the branches tend to curve down. The surface texture of the trunk is even. The tree is vigorous with dark green leaves (See
Flowering occurs at the end of March to April in Pilar de la Horadada, Alicante, Spain (See,
Leaf length on average is 10.2 cm, with a medium leaf width of 4.0 cm on average, and a large leaf ratio (2.55). There is no leaf blade or spine. The leaf has a straight shape in the cross section and an acute shape of apex. The leaf shape is elliptic (see descriptor 1 in section 7.2.9 of the IPGRI Descriptors for Citrus) and the margin type is wavy (see descriptor 4 in section 7.2.10 of the IPGRI Descriptors for Citrus). The base shape is absent (see descriptor 0 in section 7.2.12 of the IPGRI Descriptors for Citrus). The length of the petiole is medium, typically 10 mm, and wings are absent. Upper leaf surface color is typically dark green, RHS 139 A, while lower leave surface color is typically a strong yellowish green, RHS 144 C.
‘Mandarin Queen’ mandarin fruit is typically harvested middle of January to end of April in Pilar de la Horadada, Alicante, Spain. Fruit production is similar to its parent, ‘Satsuma’. Fruit production of a five year old ‘Mandarin Queen’ mandarin tree grafted in a plantation over Cleopatra rootstock is on average 75 kg per tree.
The ‘Mandarin Queen’ mandarin fruit has a short height, on average 45.2 mm, and a medium to large diameter, on average 65.9 mm. The color of the fruit surface is typically a vivid reddish orange, RHS 30A. It has a flattened general shape, absence of neck and presence of depression at stalk end. There are an intermediate number of radial grooves at the stock end, absent presence of collar, presence of depression at distal end, and complete grooved presence of the areola. There is no persistence of style. Occasionally there is the presence of a navel and, when present, the size of the navel is medium, while the bulging of the navel is intermediate to strong. The fruit rind has medium thickness, medium strength and medium oiliness, with a medium adherence to flesh, white color and loose density of albedo. The color of the albedo is typically white, RHS 155B and the presence of the albedo strand is medium present. The fruit surface texture is even (see descriptor 1 in section 7.4.12 of the IPGRI Descriptors for Citrus) and the pulp vesicles size is average. The date of maturity in Alicante, Spain is from the middle of January until the end of April.
The areola is grooved and the average diameter is from 5 mm to 15 mm. The average diameter of the stylar scar is from 5 mm to 15 mm and the protruding stylar point is large. The fruit has a strong glossy surface and the fruit's average weight is between 100 gr. to 150 gr. The size of the oil glands is typically 1 mm.
The fruit flesh has a medium orange color, large core diameter, and an average of 16 well developed segments. Specifically, the typical color of the mature internal fruit flesh is RHS 30D. The segment walls have a strong strength and there is an intermediate presence of a navel internally. There is no presence of rudimentary segments. The fruit, while virtually seedless, will occasionally have some seeds and the variety does not require pollination. Specifically, the number of seeds is absent or very few, the surface texture of the seeds is smooth and the average size of the seeds is 7 mm by 3 mm. The fruit flavor is sweet and lightly acidic, having a mandarin aroma, with soft pulp texture and thicker segment texture than a ‘Clementine’. The fruit is also high in juiciness.
Results of storage trials have indicated that the first significant signs of fruit deterioration has been found at day 37, with no post harvesting treatments (as imazalil) at +5 Celsius. Fruit of the trial had a Brix of 13 and acid of 1.05%.
No susceptibilities to plant or fruit diseases or to pests beyond those normally associated with citrus species have been observed. Specifically, ‘Mandarin Queen’ mandarin is tolerant to Citrus Tristeza Virus (CTV) and Alternaria species. Further, aphids, mites and scales affect this variety similar to ‘Clementines’. Like all varieties, only summer treatments for pests are needed.
DNA of Queen mandarin leaves was extracted using the Qiagen DNeasy Plant kit. Based on their level of polymorphism the following 32 simple sequence repeat (SSR) suitable markers were selected (see table). Extracted DNA was amplified by PCR and separated by capillary electrophoresis using a CEQ 8000 Beckman DNA analyser (Beckman). The table indicates the size of each of the two Mandarin Queen alleles of each marker. Therefore, the following primers can be used separately, or in combination, to identify ‘Mandarin Queen’ and to distinguish ‘Mandarin Queen’ from other varieties.
Sequence forward
Sequence reverse
Allele
Allele
(SEQ ID NO:)
(SEQ ID NO:)
1
2
ATCACAATTACTAGC
TTGCATTGTAGCATGT
248
248
AGCGCC (1)
TGG (2)
GGACCACAACAAAGA
TGGAGACACAAAGAAG
132
146
CAG (3)
AA (4)
GCAAAACAAGCAGAC
AGGACAGATGACCCAG
203
205
TACAAAT (5)
ATGACA (6)
AAGCATTTAGGGAGG
TGCTGCTGCTGTTGTT
216
226
GTCACT (7)
GTTCT (8)
CAGCTCAACATGAAA
TTGGAGAACAGGATGG
162
164
GG (9)
(10)
AATGATGAGGGTAAA
ACCCATCACAAAACAG
230
230
GATG (11)
A (12)
CAATAAGAAAACGCA
TGGTAGAGAAACAGAG
122
124
GG (13)
GTG (14)
CACCTTTCCCTTCCA
TGAGGGACTAAACAGC
261
265
(15)
A (16)
CAGAGACAGCCAAGA
GCTTCTTACATTCCTC
207
220
GA (17)
AAA (18)
CCACACAGGCAGACA
CCTTGGAGGAGCTTTA
198
227
(19)
C (20)
CCCAACAAACTCAAA
TTTTTATTTCGGTCTC
96
96
CTTC (21)
CTT (22)
CGGAACAACTAAAAC
TGGGCTTGTAGACAGT
104
107
AAT (23)
TA (24)
GACCCTGCCTCCAAA
GTGGCTGTTGAGGGGT
241
249
GTATC (25)
TG (26)
CCTTTTCACAGTTTG
TCAATTCCTCTAGTGT
188
188
CTAT (27)
GTGT (28)
TGTAGTCAAAAGCAT
TCTATGATTCCTGACT
118
118
CAC (29)
TTA (30)
TCCACAGATTGCCCA
CCCTAAAAACCAAGTG
151
157
TTA (31)
ACA (32)
GTTGCTGATGCTACA
CCTCTCTCTCTTCTTT
240
242
GATG (33)
ACCG(34)
CCTTATCTTCATCAC
TAAAAAGATGGGGCCT
237
239
CTCCGTC (35)
TGTG (36)
GCTGAGATGGGGATG
CCCCATCCTTTCAACT
176
176
AAAGA (37)
TGTG (38)
TCCCTATCATCGGCA
CAATAATGTTAGGCTG
181
184
ACTTC (39)
GATGGA (40)
TACCTCCACGTGTCA
GCTGTCACGTTGGGTG
141
147
AACCA (41)
TATG (42)
CGCGGATCATCTAGCA
CTTGGCACCATCAACA
215
217
TACA (43)
CATC (44)
CATTAAAATATCCGTG
GAGCAAGTGCGTTGTT
226
226
CCGC (45)
GTGT (46)
GAGCTCAAAACAATAG
CATACCTCCCCGTCCA
332
343
CCGC (47)
TCTA (48)
GAACCAGAATCAGAAC
GGTGAGCATCTGGACG
215
215
CCGA (49)
ACTT (50)
CACGCTCTTGACTTTC
CTTTGCGTGTTTGTGC
156
165
TCCC (51)
TGTT (52)
AGTCCGCCTTTGCTTT
GGTGCAAAAGAGAGCG
135
145
TTCT (53)
AGAG (54)
GAAAGGGTTACTTGAC
CTTCCCAGCTGCACAA
185
204
CAGGC (55)
GC (56)
Patent | Priority | Assignee | Title |
PP27249, | Sep 21 2012 | AGRICULTURAL RESEARCH COUNCIL | Satsuma hybrid named ‘Sonet’ |
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